Journal
VACCINE
Volume 29, Issue 23, Pages 3962-3968Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.03.079
Keywords
E. coli O157:H7; Intimin; EspB; Vaccine
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Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica, Ministerio de Ciencia y Tecnologia [PICT 05 32687]
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Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of gamma-intimin (gamma-intimin C-280) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle. (C) 2011 Elsevier Ltd. All rights reserved.
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