Article
Immunology
Samuel Cibulski, Thais Fumaco Teixeira, Ana Paula Muterle Varela, Matheus Fabiao de Lima, Gabriela Casanova, Yuri Mangueira Nascimento, Josean Fechine Tavares, Marcelo Sobral da Silva, Patricia Sesterheim, Diogo Onofre Souza, Paulo Michel Roehe, Fernando Silveira
Summary: This study characterized the saponin-rich fraction QB-80 and a nanoadjuvant IMXQB-80 prepared with QB-80 and lipids, examining their performance in experimental inactivated vaccines against ZIKV. QB-80 and IMXQB-80 significantly enhanced serum levels of anti-Zika virus antibodies and subtypes, with IMXQB-80 being as effective as QB-80 but requiring less saponins to induce immune responses.
Article
Immunology
Fernando Silveira, Mariana Rivera-Patron, Nikita Deshpande, Soledad Sienra, Jackeline Checa, Maria Moreno, Jose A. Chabalgoity, Samuel P. Cibulski, Mariana Baz
Summary: We demonstrate that adjuvanting a seasonal trivalent influenza vaccine with Quillaja brasiliensis saponin-based nanoparticles significantly improves immune responses and provides full protection against influenza virus challenge. The adjuvanted vaccine elicited high levels of neutralizing antibodies, a mixed Th1/Th2 cytokine response, and effector T cell-mediated immune responses. The viral titers in the lungs of animals receiving the adjuvanted vaccine were significantly lower, and there was no mortality observed in mice vaccinated with the adjuvanted vaccine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Yue Zhang, Mengwei Zhang, Xilan Liao, Yunsong Yu, Qing Liu, Yongwen Luo, Jun Luo, Xiaofeng Guo
Summary: Rabies is an important zoonotic disease caused by the rabies virus (RABV). Researchers have found that inserting the interleukin-25 (IL-25) gene into the genome of RABV can enhance the immune responses induced by the rabies vaccine, leading to higher levels of virus neutralizing antibodies (VNA). Mice immunized with the recombinant RABV showed a higher survival rate and milder clinical signs after challenge with CVS-11, suggesting that IL-25 can be used as a viral vaccine adjuvant.
Article
Immunology
Mariana Rivera-Patron, Maria Moreno, Mariana Baz, Paulo M. Roehe, Samuel P. Cibulski, Fernando Silveira
Summary: Vaccination is the most effective public health intervention to prevent influenza infections. The IQB90 adjuvanted influenza vaccine can trigger a strong immune response and dose-sparing effect, which is crucial for pandemic preparedness.
Article
Biochemistry & Molecular Biology
Federico Wallace, Carolina Fontana, Fernando Ferreira, Cristina Olivaro
Summary: In this study, two compounds were isolated from the aqueous extract of Quillaja brasiliensis leaves, one of which is a previously undescribed triterpene saponin (Qb1) with potential vaccine adjuvant application. The structure of Qb1 and another known triterpene saponin (S13) were elucidated.
Article
Immunology
Samuel Cibulski, Ana Paula Muterle Varela, Thais Fumaco Teixeira, Martin Pablo Cancela, Patricia Sesterheim, Diogo Onofre Souza, Paulo Michel Roehe, Fernando Silveira
Summary: The nanoadjuvant based on saponins from Quillaja brasiliensis significantly enhanced immune responses against infectious pathogens in a study evaluating an experimental Zika virus vaccine. Compared to the standard adjuvant alum, the saponin-based nanoadjuvant vaccine showed increased levels of anti-Zika virus antibodies in mice, making it a promising candidate for Zika virus vaccination.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Selvaraj Pavulraj, Rhett W. Stout, Elise D. Barras, Daniel B. Paulsen, Shafiqul I. Chowdhury
Summary: Rift Valley fever virus (RVFV) is a threat to livestock and humans. Vaccination of livestock can protect animals and eliminate the risk of human infection. Researchers have developed a new vaccine that combines RVFV proteins with a bovine immune factor, providing protection against RVFV in calves.
Article
Microbiology
Ningning Yang, Jiangwei Zhang, Mingguo Xu, Jihai Yi, Zhen Wang, Yong Wang, Chuangfu Chen
Summary: This study successfully developed BVDV VLPs using an insect baculovirus expression vector system, obtaining VLPs of high purity. Mice immunized with VLPs showed higher levels of BVDV-neutralizing antibodies compared to the control group, and had significantly lower viral loads in various organs compared to the control group in a subsequent challenge. These findings suggest that VLPs are a promising candidate vaccine for BVDV.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Wenlong Lu, Yilin Bai, Shuai Zhang, Xuyang Zhao, Jiaxin Jin, Xiaojing Zhu, Rui Wang, Yanan Wu, Angke Zhang, Gaiping Zhang, Guoqing Zhuang, Aijun Sun
Summary: African swine fever virus (ASFV) causes a severe disease in domestic pigs. In this study, a new B cell epitope in the intracellular region of a viral protein was identified and shown to induce both humoral and cellular immune responses in a mouse model. This finding provides valuable insights for the development of ASFV vaccines.
Article
Immunology
Maria G. Byazrova, Ekaterina A. Astakhova, Aygul R. Minnegalieva, Maria M. Sukhova, Artem A. Mikhailov, Alexey G. Prilipov, Andrey A. Gorchakov, Alexander Filatov
Summary: In this study, blood samples from patients who received an adenovirus-based Gam-COVID-Vac vaccine were used to investigate the influence of anti-vector antibodies on the magnitude of SARS-CoV-2-specific humoral response after booster vaccination. The results showed that high pre-boost Ad26 neutralizing antibody titers did not affect the humoral immunogenicity of the Gam-COVID-Vac boost, supporting the development of repeated immunization schedule with adenovirus-based COVID-19 vaccines.
Article
Medicine, Research & Experimental
Kristen W. Cohen, Andrew Fiore-Gartland, Stephen R. Walsh, Karina Yusim, Nicole Frahm, Marnie L. Elizaga, Janine Maenza, Hyman Scott, Kenneth H. Mayer, Paul A. Goepfert, Srilatha Edupuganti, Giuseppe Pantaleo, Julia Hutter, Daryl E. Morris, Stephen C. De Rosa, Daniel E. Geraghty, Merlin L. Robb, Nelson L. Michael, Will Fischer, Elena E. Giorgi, Harman Malhi, Michael N. Pensiero, Guido Ferrari, Georgia D. Tomaras, David C. Montefiori, Peter B. Gilbert, M. Juliana McElrath, Barton F. Haynes, Bette T. Korber, Lindsey R. Baden
Summary: This study found that vaccination with mosaic immunogens could induce more specific T cell responses and increase recognition of heterologous variants, suggesting that mosaic and consensus immunogens are promising approaches to address the global diversity of HIV-1.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Immunology
Meng-Hsuan Sung, W. Zane Billings, Michael A. Carlock, Hannah B. Hanley, Justin Bahl, Andreas Handel, Ted M. Ross, Ye Shen
Summary: The long-term effects of host factors on vaccine-induced immune responses and their interactions with annual influenza vaccinations have been explored in a cohort study of 386 individuals who received standard-dose influenza vaccine for at least 2 seasons from 2016 to 2020. The study revealed disparate immune responses between males and females in adults who were repeatedly vaccinated, as well as interactive effects between age and BMI, and between sex at birth and BMI in adults.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Virology
Pankaj Ahluwalia, Ashutosh Vashisht, Harmanpreet Singh, Nikhil Shri Sahajpal, Ashis K. Mondal, Kimya Jones, Jaspreet Farmaha, Ryan Bloomquist, Caroline Marie Carlock, Drew Fransoso, Christina Sun, Tyler Day, Comfort Prah, Trinh Vuong, Patty Ray, Danielle Bradshaw, Marisol Miranda Galvis, Sadanand Fulzele, Girindra Raval, Justin Xavier Moore, Jorge Cortes, Jeffrey N. James, Vamsi Kota, Ravindra Kolhe
Summary: This study aimed to investigate the temporal changes in the humoral immune response among healthcare workers in Augusta, GA, USA, and explore any associations with ethno-demographic features. The findings showed a significant decline in neutralizing antibody (NAb) and IgG levels at 8-12 months post-vaccination, with a more pronounced decline in White HCWs. Booster doses were found to increase antibody levels significantly, while participants without booster doses experienced a decline in antibody levels at 12 months post-vaccination.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cristina Mendes Peter, Lariane da Silva Barcelos, Marcos Roberto Alves Ferreira, Stefanie Bressan Waller, Matheus Iuri Fruhauf, Nadalin Yandra Botton, Fabricio Rochedo Conceicao, Marcelo de Lima, Silvia de Oliveira Hubner, Jose Mario Barichello, Geferson Fischer
Summary: The present study evaluated the potential of an intravaginal vaccine against bovine alphaherpesvirus type 5 (BoHV-5). Results showed that the group inoculated with BoHV-5 and rLTB via intramuscular injection (G5) demonstrated the highest levels of IgG in the vaginal mucosa. The groups inoculated with polymers (G1 and G3) showed higher levels of IgG in the vaginal mucosa compared to the positive control group.
MOLECULAR IMMUNOLOGY
(2023)
Article
Microbiology
Irina Matveeva, Olga Karpova, Nikolai Nikitin, Oleg Akilin, Vasiliy Yelnikov, Irina Litenkova, Roman Melnik, Nikolai Melnik, Karim Asimov, Aleksey Zaberezhny, Yriy Fyodorov, Evgeniya Markova
Summary: The core of reindeer rabies eradication strategy is regular vaccination to achieve and maintain sufficient coverage for stopping rabies virus transmission. The study found that a single dose of 3 ml inactivated rabies vaccine induced stable production of specific neutralizing antibodies in reindeer, and two doses with a 30-day interval established a strong immunity for at least 2 years. The data did not support the benefit of an adjuvanted vaccine for preventing rabies in reindeer.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Willian P. Paim, Fabricio S. Campos, Samuel P. Cibulski, Camila M. Scheffer, Caroline Tochetto, Ana P. M. Varela, Dennis M. Junqueira, Fabiana Q. Mayer, Phyllis C. Romijn, Edviges M. Pituco, Ana C. Franco, Fernando R. Spilki, Paulo M. Roehe
Summary: In this study, we report the complete genome sequences of two subtype c strains of bovine alphaherpesvirus 5 isolated from cattle in southeast Brazil. Limited information is currently available on subtype c.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Genetics & Heredity
Andre Alberto Witt, Raquel Silva Alves, Juliana do Canto Olegario, Laura Junqueira de Camargo, Matheus Nunes Weber, Mariana Soares da Silva, Raissa Canova, Ana Cristina Sbaraini Mosena, Samuel Paulo Cibulski, Ana Paula Muterle Varela, Fabiana Quoos Mayer, Claudio Wageck Canal, Renata da Fontoura Budaszewski
Summary: This study aimed to expand the knowledge about the viral diversity associated with white-winged vampire bats by sampling and sequencing different organs of the bats. The results revealed various viruses closely related to different virus families. The findings are important for understanding the viral diversity of this bat species and for future comparisons of virus outbreaks.
Article
Infectious Diseases
Camila Pereira Silva, Celso Jose Bruno de Oliveira, Elma Lima Leite, Samuel Paulo Cibulski, Magda Fernandes, Priscylla Carvalho Vasconcelos, Larissa Maranhao Dias, Nubia Michelle Vieira da Silva, Felicio Garino Junior, Artur Cesar de Carvalho Fernandes
Summary: This study investigated the genetic context of expanded-spectrum 13-lactam resistance in a Klebsiella pneumoniae strain causing a hard-to-treat nasal infection in a domestic cat. The strain was found to carry multiple genes conferring resistance to various antimicrobial drugs. The results highlight the importance of horizontal gene transfer in the acquisition of antimicrobial resistance genes and emphasize the need for a One Health approach to understand the emergence and dissemination of antimicrobial resistance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Materials Science, Biomaterials
Samuel Cibulski, Thalisson Amorim de Souza, Joanda Paola Raimundo, Yuri Mangueira Nascimento, Lucas Silva Abreu, Norma Suarez, Iris Miraballes, Paulo Michael Roehe, Demetrius Antonio Machado de Araujo, Josean Fechine Tavares, Marcelo Sobral da Silva, Fernando Silveira
Summary: This study presents a protocol for formulating ISCOM-matrices using raw aqueous extract of Quillaja lancifolia leaves. The method is easier, more robust, cheaper, and environmentally friendly than traditional purification methods.
Article
Virology
Ueric Jose Borges de Souza, Raissa Nunes dos Santos, Marta Giovanetti, Luiz Carlos Junior Alcantara, Jucimaria Dantas Galvao, Franciano Dias Pereira Cardoso, Feliph Cassio Sobrinho Brito, Ana Claudia Franco, Paulo Michel Roehe, Bergmann Morais Ribeiro, Fernando Rosado Spilki, Fabricio Souza Campos
Summary: This study investigates the genomic epidemiology of the CHIKV virus in Tocantins state, Brazil, and finds that the virus belongs to the ECSA lineage and is closely related to outbreaks in Northeast and Southeast Brazil. The study identifies several frequent non-synonymous mutations in the virus genome, indicating local transmission in the state. The findings highlight the importance of continued genomic surveillance for predicting new infections and improving public health services.
Article
Microbiology
Filipe Vieira Santos de Abreu, Kamila Lorene Soares Rocha, Ramon Silva-Oliveira, Mariana Viana Macedo, Thamires Gabriele Macedo Silva, Maria Eduarda Goncalves-Dos-Santos, Cirilo Henrique de Oliveira, Sandy Micaele Aquino-Teixeira, Vinicius de Oliveira Ottone, Alex Junio Jardim da Silva, Ronaldo Medeiros Dos Santos, Aline Tatila-Ferreira, Marco Antonio Barreto de Almeida, Edmilson Dos Santos, Jader da Cruz Cardoso, Aline Alves Scarpellini Campos, George Rego Albuquerque, Anaia da Paixao Seva, Bergmann Morais Ribeiro, Danilo Simonini Teixeira, Fabricio Souza Campos, Ana Claudia Franco, Paulo Michel Roehe, Giliane de Souza Trindade, Danilo Bretas de Oliveira
Summary: Evidence of Orthopoxvirus (OPXV) infection has been detected for the first time in neotropical free-living primates in southeast Brazil, indicating their previous exposure to the virus. This finding is significant for understanding the transmission and infection risks of OPXV in primates.
Article
Microbiology
Lina Violet-Lozano, Vanessa Haach, Camila Mosca Barboza, Juliana dos Santos, Bianca Ferreira Gomes, Tatiane de Cassia Pardo de Souza, Pedro Carnieli Junior, Clarice Weis Arns, Helena Beatriz de Carvalho Ruthner Batista, Paulo Michel Roehe, Ana Claudia Franco
Summary: This study aimed to evaluate the presence of influenza A virus and coronavirus in non-hematophagous bats in Brazil. However, all samples analyzed were negative, suggesting that virus circulation in bats in the wild often occurs at low viral loads.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Camila Mengue Scheffer, Ana Paula Muterle Varela, Thais Fumaco Teixeira, Candice Schmidt, Samuel Paulo Cibulski, Helton Fernandes dos Santos, Phelipe Magalhaes Duarte, Fabricio Souza Campos, Ana Claudia Franco, Paulo Michel Roehe
Summary: Water buffaloes have been introduced as a source of animal protein, but little is known about infectious diseases of them. This study investigated the neutralizing antibodies to alphaherpesviruses in bubaline sera, and found that 46.9% of the samples neutralized at least one of the viruses tested.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Virology
Cirilo H. de Oliveira, Miguel S. Andrade, Fabricio S. Campos, Jader da C. Cardoso, Maria Eduarda Goncalves-dos-Santos, Ramon Silva Oliveira, Sandy Micaele Aquino-Teixeira, Aline A. S. Campos, Marco A. B. Almeida, Danilo Simonini-Teixeira, Anaia da P. Seva, Andrea Oliveira Dias Temponi, Fernando Maria Magalhaes, Agna Soares da Silva Menezes, Bartolomeu Teixeira Lopes, Hermes P. Almeida, Ana Lucia Pedroso, Giovani Pontel Goncalves, Danielle Costa Capistrano Chaves, Givaldo Gomes de Menezes, Sofia Bernal-Valle, Nicolas F. D. Mueller, Luis Janssen, Edmilson dos Santos, Maria A. Mares-Guia, George R. Albuquerque, Alessandro P. M. Romano, Ana C. Franco, Bergmann M. Ribeiro, Paulo M. Roehe, Ricardo Lourenco-de-Oliveira, Filipe Vieira Santos de Abreu
Summary: In a survey conducted in the Cerrado areas of Minas Gerais, Brazil, it was found that mosquitoes of the Sabethes genus, particularly Sabethes chloropterus, were the primary vectors involved in maintaining the yellow fever virus (YFV) in semi-arid environments. The high number of YFV RNA copies and relative abundance in Sa. chloropterus indicate its importance as a vector, while Sa. albiprivus, despite being naturally infected with YFV, had fewer viral RNA copies and a lower infection rate.
Article
Immunology
Fernando Silveira, Mariana Rivera-Patron, Nikita Deshpande, Soledad Sienra, Jackeline Checa, Maria Moreno, Jose A. Chabalgoity, Samuel P. Cibulski, Mariana Baz
Summary: We demonstrate that adjuvanting a seasonal trivalent influenza vaccine with Quillaja brasiliensis saponin-based nanoparticles significantly improves immune responses and provides full protection against influenza virus challenge. The adjuvanted vaccine elicited high levels of neutralizing antibodies, a mixed Th1/Th2 cytokine response, and effector T cell-mediated immune responses. The viral titers in the lungs of animals receiving the adjuvanted vaccine were significantly lower, and there was no mortality observed in mice vaccinated with the adjuvanted vaccine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Veterinary Sciences
Camila Mengue Scheffer, Sylio Alfredo Petzhold, Ana Paula Muterle Varela, Willian Pinto Paim, Phelipe Magalhaes Duarte, Marcia Regina Loiko, Cristine Cerva, Candice Schmidt, Adrieli Wendlant, Samuel Paulo Cibulski, Diane Alves de Lima, Caroline Tochetto, Anne Caroline Ramos dos Santos, Juliana Ines Herpich, Thais Fumaco Teixeira, Helton Fernandes dos Santos, Fabricio Souza Campos, Ana Claudia Franco, Paulo Michel Roehe
Summary: A highly sensitive and specific multi-antigen ELISA method has been developed for detecting antibodies to Bovine alphaherpesviruses type 1, type 5, and bubaline herpesvirus type 1. The sensitivity and specificity of this method are comparable to serum neutralization tests.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Tatiana R. Vieira, Esther F. Cavinatto de Oliveira, Samuel P. Cibulski, Nubia M. V. Silva, Mauro R. Borba, Celso J. B. Oliveira, Marisa Cardoso
Summary: This study investigated the microbial composition, abundance of antimicrobial resistance genes (ARGs), and mobile genetic elements (MGEs) in retail chicken carcasses from different production systems. Although there were no differences in microbial diversity, certain taxa, including spoilage-associated organisms, showed significant differences across the groups. The co-resistome comprised 29 shared ARGs, and their abundance was lower in chicken carcasses from certified antimicrobial-free intensive production systems (AF) and certified organic production systems with restricted antimicrobial use (OR) compared to conventional intensive production systems (CO). Transposases were the most abundant MGEs, with a higher abundance in CO compared to OR.
Article
Agriculture, Multidisciplinary
Tatiana Regina Vieira, Esther Cavinatto de Oliveira, Samuel Paulo Cibulski, Mauro Riegert Borba, Marisa Cardoso
Summary: This study assessed the antimicrobial resistance profiles of Escherichia coli isolated from whole chickens originating from farming systems with and without restrictions on the use of antimicrobials. The results showed that the chicken meat from farming systems with restrictions on antimicrobial use had lower resistance to antibiotics, indicating lower selection pressure for antimicrobial resistance.
SEMINA-CIENCIAS AGRARIAS
(2022)
