Article
Immunology
Carine Ysebaert, Cindy Castado, Marie-Cecile Mortier, Stephane Rioux, Christiane Feron, Emmanuel Di Paolo, Vincent Weynants, Normand Blais, Nathalie Devos, Philippe Hermand
Summary: Mcat is a key pathogen in exacerbations of COPD in adults and otitis media in children. Vaccines targeting UspA2 as a potential antigen showed promising results in animal models and bactericidal assays, indicating its potential as a vaccine candidate. Further studies are needed to explore the efficacy of UspA2 vaccination in preventing Mcat infections.
Article
Immunology
Mohamed A. Soltan, Nada Elbassiouny, Helmy Gamal, Eslam B. Elkaeed, Refaat A. Eid, Muhammad Alaa Eldeen, Ahmed A. Al-Karmalawy
Summary: A novel multitope vaccine was designed based on epitopes filtered from the entire proteome of M. catarrhalis, demonstrating potential effectiveness in triggering a specific immune response. This vaccine includes essential, outer membrane proteins with high antigenicity scores and covers conserved regions, along with adjuvants for improved immune stimulation. Further translational research is necessary to validate the efficacy of this designed vaccine.
Article
Immunology
Uwa Iyamu, Daniel Ferrer Vinals, Bernard Tornyigah, Eliana Arango, Rakesh Bhat, Trixie Rae Adra, Simranjit Grewal, Kimberly Martin, Amanda Maestre, Michael Overduin, Bart Hazes, Stephanie K. K. Yanow
Summary: During Plasmodium falciparum infection in pregnancy, VAR2CSA expressed on infected erythrocytes (IEs) mediates their sequestration in the placenta. However, we discovered that antibodies to VAR2CSA can also be elicited by P. vivax Duffy binding protein (PvDBP). These findings suggest that PvDBP-derived antibodies may cross-react with VAR2CSA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Dustin M. McCraw, Mallory L. Myers, Neetu M. Gulati, Madhu Prabhakaran, Joshua Brand, Sarah Andrews, John R. Gallagher, Samantha Maldonado-Puga, Alexander J. Kim, Udana Torian, Hubza Syeda, Seyhan Boyoglu-Barnum, Masaru Kanekiyo, Adrian B. McDermott, Audray K. Harris
Summary: Structure-guided design of nanoparticles displaying conserved regions of influenza virus has the potential to develop more efficacious and universal vaccines. This design concept provides a new approach to universal influenza vaccine development strategies and opens opportunities for the development of nanoparticles with broad coverage over many antigenically diverse influenza HA subtypes.
Article
Immunology
Sian E. Jossi, Melissa Arcuri, Areej Alshayea, Ruby R. Persaud, Edith Marcial-Juarez, Elena Palmieri, Roberta Di Benedetto, Marisol Perez-Toledo, Jamie Pillaye, Will M. Channell, Anna E. Schager, Rachel E. Lamerton, Charlotte N. Cook, Margaret Goodall, Takeshi Haneda, Andreas J. Baumler, Lucy H. Jackson-Jones, Kai-Michael Toellner, Calman A. MacLennan, Ian R. Henderson, Francesca Micoli, Adam F. Cunningham
Summary: Vi-specific IgM or IgG can independently protect against infection, and superior protection from vaccination with TCV in adults may be related to the ability of the response to persist better.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Bing Li, Jing Zhang, Yang Huang, Xinrui Li, Jing Feng, Yan Li, Rongxin Zhang
Summary: This study developed a SARS-CoV-2 N-Ferritin nanoparticle vaccine by fusing the SARS-CoV-2 N protein with Helicobacter pylori nonheme ferritin. The N-Ferritin nanoparticles induced a stronger immune response in mice compared to the monomer N protein, resulting in more antibodies and better cross-reactivity.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Medicinal
Logan R. Wilks, Gaurav Joshi, Megan R. Grisham, Harvinder Singh Gill
Summary: This study describes a method of creating nanoclusters (NCs) from soluble peptide molecules using a tyrosine-tyrosine cross-linking reaction. The introduction of a reactive tag comprising histidine and tyrosine residues at the termini of the peptide molecules led to the formation of peptide NCs. The study demonstrates the importance of reaction conditions on final cluster diameter and the ability to generate NCs of various sizes. Furthermore, the created peptide clusters show promising immunogenic potential against a lethal influenza A challenge in BALB/c mice.
ACS INFECTIOUS DISEASES
(2021)
Article
Virology
Minchao Li, Jinfeng Zeng, Ruiting Li, Ziyu Wen, Yanhui Cai, Jeffrey Wallin, Yuelong Shu, Xiangjun Du, Caijun Sun
Summary: Developing a global HCoVs vaccine is crucial in the field of public health with the rapid spread of COVID-19. The novel antigen design with broad coverage has the potential to induce strong CTL responses.
Review
Immunology
Javid Aceil, Fikri Y. Avci
Summary: This review provides an overview of multiple classes of conserved surface proteins as vaccine targets for Streptococcus pneumoniae, discussing their contribution to the virulence of pneumococcal disease and their potential as vaccine targets.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Song Bai, Hang Gao, Lin-Tong-Qing Tan, Lulu Yao, Xin Meng, Yongzhong Zhang
Summary: In this study, a novel conjugate vaccine Tn-BSA-CTS with chitotriose as built-in adjuvant was synthesized, and the effect of adjuvant chitotriose was evaluated. The immunological evaluations showed that Tn-BSA-CTS could stimulate the highest titers of IgG antibodies (102,400) and significantly enhance both humoral and cellular immunity. These results demonstrate the potential of CTS as a novel vaccine adjuvant and suggest that the covalent linkage of tumor vaccine to CTS could be an effective strategy to enhance the efficacy against cancer.
CARBOHYDRATE RESEARCH
(2023)
Article
Engineering, Chemical
Yanan Sheng, Jiangxue Wei, Zhengjun Li, Zhiguo Su, Guanghui Ma, Songping Zhang
Summary: In this study, ferritin nanoparticles were used as a carrier for influenza vaccine by chemically conjugating M2e and HA antigens on the outer surface. The dual-antigen vaccine candidate showed high levels of antigen-specific antibodies in immunized mice and provided effective protection against both homologous and heterologous influenza virus strains.
Article
Multidisciplinary Sciences
Flavia Chiuppesi, John A. Zaia, Katelyn Faircloth, Daisy Johnson, Minh Ly, Veronica Karpinski, Corinna La Rosa, Jennifer Drake, Joan Marcia, Ann Marie Acosta, Shannon Dempsey, Randy A. Taplitz, Qiao Zhou, Yoonsuh Park, Sandra Ortega Francisco, Teodora Kaltcheva, Paul H. Frankel, Steven Rosen, Felix Wussow, Sanjeet Dadwal, Don J. Diamond
Summary: Individuals vaccinated with COH04S1 or mRNA vaccine BNT162b2 maintain robust cross-reactive cellular immunity for six or more months post-vaccination, providing long-term protection against both ancestral and variant strains of SARS-CoV-2.
Article
Immunology
Shahira Shahid, Amala Khan, Muhammad Imran Nisar, Farah Khalid, Muhammad Farrukh Qazi, Sheraz Ahmed, Furqan Kabir, Aneeta Hotwani, Sahrish Muneer, Syed Asad Ali, Cynthia G. Whitney, Anita K. M. Zaidi, Fyezah Jehan
Summary: The 10-valent pneumococcal vaccine was introduced in Pakistan's Expanded Program on Immunization (EPI) in 2013. Three annual cross-sectional surveys were conducted from 2014-2016, showing a significant decrease in vaccine-type carriage in infants.
Article
Biochemistry & Molecular Biology
Daria Mezhenskaya, Irina Isakova-Sivak, Tatiana Gupalova, Elena Bormotova, Eugenia Kuleshevich, Tatiana Kramskaya, Galina Leontieva, Larisa Rudenko, Alexander Suvorov
Summary: This study investigates the feasibility of using Enterococcus faecium L3 as a bacterial vector for oral immunization against influenza virus. The results demonstrate that the L3-LAH+4M2e vaccine shows superior immunogenicity and protective potential in mice.
Article
Immunology
Raul Isturiz, Lindsay Grant, Sharon Gray, Ronika Alexander-Parrish, Qin Jiang, Luis Jodar, Paula Peyrani, Kimbal D. Ford, Michael W. Pride, Wesley H. Self, Francis Counselman, Gregory Volturo, Luis Ostrosky-Zeichner, Richard G. Wunderink, Robert Sherwin, J. Scott Overcash, Thomas File, Julio Ramirez
Summary: This study identified a significant proportion of hospitalized adult CAP cases associated with PCV20-unique serotypes, showing the potential of PCV20 in addressing the burden of CAP caused by additional serotypes not covered by PCV13. UAD2 detection technology played a crucial role in identifying and differentiating these serotypes.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Artur Muszynski, Christian Heiss, Christian T. Hjuler, John T. Sullivan, Simon J. Kelly, Mikkel B. Thygesen, Jens Stougaard, Parastoo Azadi, Russell W. Carlson, Clive W. Ronson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Multidisciplinary Sciences
Jeannette N. Rapicavoli, Barbara Blanco-Ulate, Artur Muszynski, Rosa Figueroa-Balderas, Abraham Morales-Cruz, Parastoo Azadi, Justyna M. Dobruchowska, Claudia Castro, Dario Cantu, M. Caroline Roper
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Xue Kang, Alex Kirui, Artur Muszynski, Malitha C. Dickwella Widanage, Adrian Chen, Parastoo Azadi, Ping Wang, Frederic Mentink-Vigier, Tuo Wang
NATURE COMMUNICATIONS
(2018)
Article
Microbiology
Michael R. Davis, Artur Muszynski, Ivonne V. Lollett, Christopher L. Pritchett, Russell W. Carlson, Joanna B. Goldberg
JOURNAL OF BACTERIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Dusty B. Brown, Artur Muszynski, Omar Salas, Kacie Speed, Russell W. Carlson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Microbiology
Song Gao, Dabin Ren, Daxin Peng, Wenhong Zhang, Artur Muszynski, Russell W. Carlson, Xin-Xing Gu
JOURNAL OF MEDICAL MICROBIOLOGY
(2013)
Article
Microbiology
Artur Muszynski, Kamila Rabsztyn, Katarzyna Knapska, Katarzyna A. Duda, Katarzyna Duda-Grychtol, Katarzyna Kasperkiewicz, Joanna Radziejewska-Lebrecht, Otto Holst, Mikael Skurnik
Article
Multidisciplinary Sciences
Jaslyn E. M. M. Wong, Kira Gysel, Thea G. Birkefeldt, Maria Vinther, Artur Muszynski, Parastoo Azadi, Nick S. Laursen, John T. Sullivan, Clive W. Ronson, Jens Stougaard, Kasper R. Andersen
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Artur Muszynski, Kol A. Zarember, Christian Heiss, Joseph Shiloach, Lars J. Berg, John Audley, Arina Kozyr, David E. Greenberg, Steven M. Holland, Harry L. Malech, Parastoo Azadi, Russell W. Carlson, John Gallin
Summary: Granulibacter bethesdensis can infect patients with chronic granulomatous disease by its unique lipopolysaccharide structure characterized by strong acid resistance and low proinflammatory potency, potentially contributing to immune evasion and resistance to antimicrobial peptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Clay S. Crippen, Bibi Zhou, Silke Andresen, Robert T. Patry, Artur Muszynski, Craig T. Parker, Kerry K. Cooper, Christine M. Szymanski
Summary: This study isolated new Acinetobacter-infecting phages and determined their cell receptors. Additionally, a new genus of Cystoviruses was described and the membrane-associated glycoconjugates were characterized.
Article
Biochemistry & Molecular Biology
Ian M. Black, Christian Heiss, Mukesh Jain, Artur Muszynski, Russell W. Carlson, Dean W. Gabriel, Parastoo Azadi
Summary: Huanglongbing disease, caused by the unculturable bacterium Candidatus Liberibacter asiaticus, has devastated the citrus industry in Florida. Studying a related cultivable bacterium, Liberibacter crescens, provides insights into how the Liberibacter genus attacks citrus trees and offers potential strategies for managing the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Alexandre Poulhazan, Malitha C. Dickwella Widanage, Artur Muszynprimeski, Alexandre A. Arnold, Dror E. Warschawski, Parastoo Azadi, Isabelle Marcotte, Tuo Wang
Summary: The article presents a widely applicable protocol for identifying and quantifying the carbohydrate content of microalgae using magic-angle-spinning solid-state NMR spectroscopy, with validation from mass-spectrometry analysis; it is found that starch is the most abundant polysaccharide in a cellulose-deficient microalga, adopting an organized and rigid structure in the cell; the research suggests that most carbohydrates in the cell wall of the microalga are largely mobile.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Nicholas Spellmon, Artur Muszynski, Ireneusz Gorniak, Jiri Vlach, David Hahn, Parastoo Azadi, Jochen Zimmer
Summary: In this study, the authors identified the structure of O antigen with methylated mannose or rhamnose as its cap and revealed how WzmWzt recognizes and interacts with the O antigen. The findings have important implications for understanding the critical steps in the recognition and translocation of polysaccharides by ABC transporters.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jinge Liu, Ting Wang, Qiulin Qin, Xiaocheng Yu, Shengming Yang, Randy D. Dinkins, Anett Kuczmog, Peter Putnoky, Artur Muszynski, Joel S. Griffitts, Attila Kereszt, Hongyan Zhu
Summary: Plants have evolved the ability to distinguish between symbiotic and pathogenic microbial signals. The NS1 locus in Medicago truncatula can block the invasion and nodule induction by Sinorhizobium meliloti. The NS1 locus is controlled by two genes, NS1a and NS1b, which can regulate the nodulation blockade of specific bacterial strains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Yanjun Yang, Beibei Xu, James Haverstick, Nabil Ibtehaz, Artur Muszynski, Xianyan Chen, Muhammad E. H. Chowdhury, Susu M. Zughaier, Yiping Zhao
Summary: The study successfully differentiated and classified bacterial endotoxins using surface enhanced Raman scattering spectra, achieving 100% accuracy with the RamanNet algorithm. This approach has the potential for rapid medical diagnosis and therapeutic decision-making.