Journal
VACCINE
Volume 29, Issue 15, Pages 2742-2748Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.01.089
Keywords
Toxoplasma gondii; Nucleoside triphosphate hydrolase; Th1/Th2
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Funding
- Science and Technology Department of Zhejiang Province of China [2009C33035]
- Natural Scientific Foundation of China [31030066]
- GDUPS
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The Toxoplasma gondii nucleoside triphosphate hydrolase (TgNTPase) has apyrase activity, degrading ATP to the di- and mono-phosphate forms and may be used by the parasite to salvage purines from the host cell for survival and replication. To study the immune-protective value of TgNTPase-II, BALB/c mice were immunized with a recombinant form of the antigen rTgNTPase-II combined with alum. All immunized mice produced specific anti-rTgNTPase-II immunoglobulins, with high IgG antibody titers and a mixed IgG1/IgG2a response, with predominance of IgG2a production. The cellular immune response was associated with the production of IFN-gamma and IL-2 cytokines and the increase of the percentage of CD8+ T cells. Vaccinated mice displayed significant protection against acute infection with the virulent RH strain (P < 0.05 in survival rate) and also chronic infection with PRU cyst (62.9% and 57.6% reduction in brain parasite load for rTgNTPase-II + alum and rTgNTPase-II alone vaccinated groups) compared to the non-vaccinated control group. In conclusion, rTgNTPase-II elicits a strong specific Th1 immune response providing partial protection against both T. gondii acute and chronic infection. (C) 2011 Elsevier Ltd. All rights reserved.
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