Article
Immunology
Hana M. El Sahly, Robert L. Atmar, Eli Sendra, Ashley Wegel, Wendy A. Keitel
Summary: The study concluded that the combination of topical imiquimod with ID MIV A/H5N1 was safe but did not result in improved serologic responses to the vaccine.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Zhuofan Li, Ki-Hye Kim, Noopur Bhatnagar, Bo Ryoung Park, Subbiah Jeeva, Yu-Jin Jung, Jannatul Raha, Sang-Moo Kang, Xinyuan Chen
Summary: This study presents a physical radiofrequency adjuvant (RFA) that can conveniently and effectively enhance the immunogenicity and efficacy of H5N1 vaccine. The RFA treatment on the skin induces thermal stress to enhance immune responses and does not cause significant adverse reactions. The RFA significantly increases antibody titers, reduces viral titers, body weight loss, and improves survival rates in murine models. It has no gender difference in its effects.
Article
Immunology
Ariel Isaacs, Zheyi Li, Stacey T. M. Cheung, Danushka K. Wijesundara, Christopher L. D. McMillan, Naphak Modhiran, Paul R. Young, Charani Ranasinghe, Daniel Watterson, Keith J. Chappell
Summary: This study tested several adjuvants for their effectiveness in inducing humoral responses to influenza and RSV subunit vaccines in mice. While all adjuvants were able to induce potent neutralizing antibodies, none significantly increased the frequency of CD4(+) and CD8(+) IFN-γ(+) cells compared to unadjuvanted antigen. The varying responses to antigens with each adjuvant suggest that optimal adjuvant selection may depend on the specific antigen and desired immune response.
Article
Immunology
Xinyu Zhuang, Luer Chen, Songhui Yang, Shengnan Xia, Zhiqiang Xu, Tong Zhang, Boyu Zeng, Tong Yu, Ning Yu, Wei Wang, Huijun Lu, Mingyao Tian, Ningyi Jin
Summary: This study investigates the design strategy of mRNA vaccines against the H5N1 influenza virus. It suggests that incorporating ferritin into the vaccine can enhance the expression of target antigens, and the addition of adjuvants can improve the immune response in mice.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Weigang Ren, Shuli Pei, Wenming Jiang, Meixia Zhao, Le Jiang, Honggang Liu, Yongxiang Yi, Mizhou Hui, Junwei Li
Summary: A replication-deficient recombinant influenza virus was developed in this study, which stimulated robust immune response and conferred complete protection against H5N1 or H9N2 influenza virus infections in mice.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Yinyan Yin, Jinyuan Wang, Xing Xu, Bangyue Zhou, Sujuan Chen, Tao Qin, Daxin Peng
Summary: Intranasal immunization with riboflavin as a mucosal adjuvant enhances mucosal immunity and systemic immune response for H1N1 WIV, while also improving the protection level against lethal influenza challenge. Riboflavin has the potential to induce dendritic cell maturation and cytokine secretion, and activates the MAPK signaling pathway, suggesting it as a promising mucosal adjuvant for influenza nasal vaccine.
Article
Biotechnology & Applied Microbiology
Ke Zhang, Xiaoxue Wu, Yu Shi, Xiaoqin Gou, Junqiong Huang
Summary: A meta-analysis was conducted on the immunogenicity of H5N1 influenza vaccines in elderly adults. The results showed that two doses of vaccines resulted in higher antibody responses, and the use of Oil-in-water emulsion (OE)-adjuvanted 7.5 μg vaccine was more effective than 3.75 μg vaccine.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Immunology
Sara H. Mahmoud, Ahmed A. Khalil, Noura M. Abo Shama, Marwa F. El Sayed, Reem A. Soliman, Naglaa M. Hagag, Nahed Yehia, Mahmoud M. Naguib, Abdel-Sattar Arafa, Mohamed A. Ali, Mounir M. El-Safty, Ahmed Mostafa
Summary: Controlling avian influenza viruses (AIVs) is mainly done through culling infected bird flocks or using inactivated vaccines. New avian influenza virus subtypes have been reported in Egypt, calling for an updated vaccine. In this study, three H5Nx vaccines were generated using reverse genetic system, and their immunogenicity and cross-reactivity were assessed. The results showed that rgH5N1_2.3.4.4 vaccine induced high immunogenicity and provided efficient protection against different H5Nx HPAIVs.
Article
Immunology
Xinliang Kang, Yibo Li, Yiwen Zhao, Xinyuan Chen
Summary: The study demonstrates that CpG 1018 adjuvant significantly enhances influenza vaccine efficacy in aged mice and helps induce antibody responses against influenza virus. This provides important insights for boosting immune responses and improving influenza vaccine effectiveness in the elderly population.
Article
Virology
Mathew Abraham, Ashley C. Beavis, Peng Xiao, Francois J. Villinger, Zhuo Li, Cheryl A. Jones, S. Mark Tompkins, Biao He
Summary: H5N1, a highly pathogenic avian influenza virus, circulates in many Asian countries and a safe and effective vaccine is needed to prevent outbreaks in humans. JPV, a nonsegmented negative-strand RNA virus, has been explored as a promising vaccine vector and has shown efficacy in inducing immune responses in animals. NNSVs like JPV can stably express foreign genes and replicate efficiently in the respiratory tract.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Pornthep Chanthavanich, Eve Versage, Esther Van Twuijver, Matthew Hohenboken
Summary: The study showed that administering the MF59-adjuvanted, cell culture-derived A/H5N1 influenza vaccine to children aged 6 months through 17 years resulted in significantly increased immunogenicity against heterologous strains, with the highest responses observed against the A/H5N1 Egypt/2010 strain.
Article
Veterinary Sciences
Jiao Hu, Peipei Peng, Jun Li, Qi Zhang, Rumeng Li, Xiaoquan Wang, Min Gu, Zenglei Hu, Shunlin Hu, Xiaowen Liu, Xinan Jiao, Daxin Peng, Xiufan Liu
Summary: The bivalent H5+H7 VLP vaccine candidate shows promising results in protecting poultry against highly pathogenic H5N1 and H7N9 avian influenza viruses, and may serve as a critical alternative to traditional egg-based inactivated vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Shi-Jian Song, Gyeong-Im Shin, Jinyong Noh, Jiho Lee, Deok-Hwan Kim, Gyeongryul Ryu, Gyeongik Ahn, Hyungmin Jeon, Hai-Ping Diao, Youngmin Park, Min Gab Kim, Woe-Yeon Kim, Young-Jin Kim, Eun-Ju Sohn, Chang Seon Song, Inhwan Hwang
Summary: The study developed a new subunit vaccine platform by expressing HA ectodomains of influenza viruses in plants, achieving potent, adjuvant-free, and multivalent vaccination. The platform successfully demonstrated strong immune responses in mice and chickens and proposed combining plant-based antigen production with BLP-based delivery as a cost-effective approach for multivalent subunit vaccines.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Danping Zhao, Xiuhong Chen, Linyuan Wang, Jianjun Zhang, Zhongpeng Zhao, Na Yue, Yingli Zhu, Wenting Fei, Xinyu Li, Lingyun Tan, Wei He
Summary: Respiratory viral infections, including COVID-19 and influenza, have become a global health concern. Vaccination is crucial for prevention, and this study evaluated the effectiveness of Astragalus polysaccharide (APS) as an immune adjuvant for influenza and COVID-19 vaccines. The results showed that APS facilitated a strong immune response in mice, with increased levels of antibodies and protection against influenza A viruses. APS also demonstrated bidirectional immunomodulation and persistent immunity, making it a promising adjuvant.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Cole J. Batty, Matthew D. Gallovic, Jonathan Williams, Ted M. Ross, Eric M. Bachelder, Kristy M. Ainslie
Summary: This study reports a new method of encapsulating an interferon gene stimulator using electrospray, which significantly improves its biological activity. A multiplexed electrospray apparatus with higher throughput than a single-head apparatus was also developed. In vitro and in vivo experiments demonstrated that microparticles produced using this high throughput process are an effective vaccine adjuvant to induce a balanced immune response.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Kan Kaneko, Eliane N. Miyaji, Viviane M. Goncalves, Daniela M. Ferreira, Carla Solorzano, Ronan MacLoughlin, Imran Saleem
Summary: This study investigated variations in chitosan-coated PLGA nanoparticles and found that PLGA variants with ester-terminal groups and negatively charged chitosans had the greatest impact on vaccine characteristics. The results suggested that NPs made from PLGA with an acid terminated group and chitosan HCl salt may be the most suitable choice for vaccine formulation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Multidisciplinary Sciences
Adriano P. Araujo, Gabriela B. C. Colichio, Maria Leonor S. Oliveira, Esther German, Elissavet Nikolaou, Tao Chen, Hugh Adler, Daniela M. Ferreira, Eliane N. Miyaji
Summary: This study reveals a decrease in serum IgG antibodies against PspA and PspC variants with age in older adults, particularly targeting the most common variants. Younger individuals showed an increase in IgG levels post-challenge for certain more cross-reactive PspA variants, as well as an increase in serum IgG against all PspC variants tested after colonization.
Article
Infectious Diseases
Nivison Nery, Juan P. Aguilar Ticona, Claudia Gambrah, Simon Doss-Gollin, Adeolu Aromolaran, Valmir Rastely-Junior, Millani Lessa, Gielson A. Sacramento, Jaqueline S. Cruz, Daiana de Oliveira, Laiara Lopes dos Santos, Crislaine G. da Silva, Viviane F. Botosso, Camila P. Soares, Danielle Bastos Araujo, Danielle B. Oliveira, Rubens Prince dos Santos Alves, Robert Andreata-Santos, Edison L. Durigon, Luis Carlos de Souza Ferreira, Elsio A. Wunder, Ricardo Khouri, Jamary Oliveira-Filho, Isadora C. de Siqueira, Antonio R. P. Almeida, Mitermayer G. Reis, Albert I. Ko, Federico Costa
Summary: This study aimed to investigate the sociodemographic determinants associated with exposure to Zika Virus (ZIKV) in pregnant women during the 2015-2016 epidemic in Salvador, Brazil. Lower education level, food insecurity, and younger maternal age were found to be risk factors for ZIKV infection among pregnant women. These findings highlight the importance of addressing social determinants in interventions to reduce the impact of ZIKV infection in vulnerable populations.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biology
Felipe Grabarz, Alexandre Paulo Yague Lopes, Flavia Ferreira Barbosa, Giovana Cappio Barazzone, Jademilson Celestino Santos, Viviane Fongaro Botosso, Soraia Attie Calil Jorge, Ana Lucia Tabet Oller Nascimento, Renato Mancini Astray, Viviane Maimoni Goncalves
Summary: Biopharmaceutical production is a multibillion-dollar industry with high growth prospects. Researchers improved soluble cIFN production by using two solubility tags and optimizing culture conditions. The biological activity of cIFN against various viruses was demonstrated, showing its potential for clinical use.
Article
Food Science & Technology
Daniela Luz, Fernando D. Gomez, Raissa L. Ferreira, Bruna S. Melo, Beatriz E. C. Guth, Wagner Quintilio, Ana Maria Moro, Agostina Presta, Flavia Sacerdoti, Cristina Ibarra, Gang Chen, Sachdev S. Sidhu, Maria Marta Amaral, Roxane M. F. Piazza
Summary: A recombinant FabF8:Stx2 antibody was successfully selected from a human Fab antibody library, showing significant neutralizing and protective effects against Stx2 from various STEC strains. This novel anti-Stx2 biomolecule has the potential to be a new therapeutic option for improving outcomes in STEC and HUS patients.
Review
Chemistry, Multidisciplinary
Marcia Carvalho de Abreu Fantini, Cristiano Luis Pinto Oliveira, Jose Luiz de Souza Lopes, Tereza da Silva Martins, Milena Apetito Akamatsu, Aryene Goes Trezena, Milene Tino-De-Franco, Viviane Fongaro Botosso, Osvaldo Augusto Brazil Esteves Sant'Anna, Nikolay Kardjilov, Martin Kjaerulf Rasmussen, Heloisa Nunes Bordallo
Summary: This article summarizes the progress made in oral vaccine formulations using porous silica matrices for antigen encapsulation. These vaccine vehicles effectively protect the antigens from stomach acid and enhance immunity. The research conducted at the Butantan Institute in Brazil is highlighted, with a focus on optimizing antigen encapsulation yield and distribution inside the silica matrix. Structural characterization and modeling were performed using various techniques, revealing the efficacy and stability of the proposed oral immunogenic complex.
Review
Immunology
Giuliana S. Oliveira, Maria Leonor S. Oliveira, Eliane N. Miyaji, Tasson C. Rodrigues
Summary: Streptococcus pneumoniae is an important pathogen, and pneumococcal conjugate vaccines have a significant impact on invasive diseases. Serotype replacement may occur after vaccine introduction, but this issue could possibly be addressed by developing serotype-independent vaccines.
Article
Physics, Condensed Matter
C. L. P. Oliveira, J. L. S. Lopes, O. A. Sant'Anna, V. F. Botosso, H. N. Bordallo, M. C. A. Fantini
Summary: Ordered mesoporous silica is an efficient oral adjuvant for delivering a variety of antigens. It has shown promising results in inducing immune response for different antigens without toxicity. Physics methods have been used to characterize the material and determine the ideal antigen load and release rate for developing proper oral vaccines.
JOURNAL OF PHYSICS-CONDENSED MATTER
(2022)
Article
Multidisciplinary Sciences
Viviane Fongaro Botosso, Soraia Attie Calil Jorge, Renato Mancini Astray, Ana Marcia de Sa Guimaraes, Monica Beatriz Mathor, Patricia dos Santos de Carneiro, Edison Luiz Durigon, Dimas Covas, Danielle Bruna Leal de Oliveira, Ricardo das Neves Oliveira, Durvanei Augusto Maria, Silas Fernandes Eto, Neuza Maria Frazatti Gallina, Giselle Pidde, Carla Cristina Squaiella-Baptistao, Dilza Trevisan Silva, Isadora Maria Villas-Boas, Dayanne Carla Fernandes, Aline Vivian Vatti Auada, Alexandre Campos Banari, Antonio Francisco de Souza Filho, Camila Bianconi, Carla Lilian de Agostini Utescher, Denise Cristina Andre Oliveira, Douglas Oscar Ceolin Mariano, Flavia Ferreira Barbosa, Giuliana Rondon, Josana Kapronezai, Juliana Galvao da Silva, Mauricio Barbugiani Goldfeder, Priscila Comone, Regis Edgar Castilho Junior, Taiana Taina Silva Pereira, Fan Hui Wen, Denise Tambourgi, Ana Marisa Chudzinski-Tavassi
Summary: The new outbreak of COVID-19 has caused millions of deaths worldwide. Efforts are being made to find effective treatments, such as using immunoglobulin from immunized animals or plasma from convalescent patients. Clinical trials using anti-SARS-CoV-2 immunoglobulins from horses immunized with spike protein are underway. Researchers have developed an anti-SARS-CoV-2 equine F(ab ')(2) immunoglobulin that successfully neutralizes the virus, is safe in animal models, and reduces the severity of the disease in a hamster model of SARS-CoV-2 infection.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Douglas Borges de Figueiredo, Kan Kaneko, Tasson da Costa Rodrigues, Ronan MacLoughlin, Eliane Namie Miyaji, Imran Saleem, Viviane Maimoni Goncalves
Summary: Pneumococcal disease is a global burden, and the current vaccines are becoming less effective due to serotype replacement. This study investigated the use of polymeric nanoparticles to deliver PspA, a candidate for new pneumococcal vaccines, and found that PGA-co-PDL/HCl-CS or PLGA/HCl-CS nanoparticles with encapsulated or adsorbed PspA provided serotype-independent protection.
Article
Biochemistry & Molecular Biology
Thatiana Correa de Melo, Dilza Trevisan-Silva, Miryam P. Alvarez-Flores, Renata Nascimento Gomes, Marcelo Medina de Souza, Hellen Paula Valerio, Douglas S. Oliveira, Carlos DeOcesano-Pereira, Viviane Fongaro Botosso, Soraia Attie Calil Jorge, Mirta Schattner, Ricardo M. Gomez, Ana Marisa Chudzinski-Tavassi
Summary: The study aimed to identify proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, in order to unravel the molecular mechanisms of endothelium activation in COVID-19. The results showed that HUVECs exhibited non-productive infection without specific cell receptor expression, and highlighted the involvement of vascular damage and oxidative stress-related protein changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Tania Maria Manieri, Daniela Yumi Takata, Roselaine Campos Targino, Wagner Quintilio, Joao Victor Batalha-Carvalho, Camila Maria Lucia da Silva, Ana Maria Moro
Summary: This study identified a panel of neutralizing monoclonal antibodies (mAbs) against tetanus toxin (TeNT), some of which can neutralize the toxin and prevent symptoms and death in mice. Two mAbs were found to impair the binding of TeNT to the GT1b ganglioside receptor. Stable cell lines were generated to produce large quantities of mAbs, and their characteristics were analyzed.
Article
Chemistry, Analytical
Karla R. Castro, Grazielle O. Setti, Tassia R. de Oliveira, Monica J. Rodrigues-Jesus, Viviane F. Botosso, Ana P. Perini de Araujo, Edson L. Durigon, Luis C. S. Ferreira, Ronaldo C. Faria
Summary: An electrochemical immunoassay using magnetic beads and enzyme-labeled antibodies was developed for the detection of anti-ZIKV antibodies. The method showed high efficiency in detecting anti-ZIKV antibodies in serum samples, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV.
Article
Immunology
Giovanna Brito Carneiro, Julia Tavares Castro, Marilyne Davi, Eliane Namie Miyaji, Daniel Ladant, Maria Leonor Sarno Oliveira
Summary: Streptococcus pneumoniae is a common pathogen causing important human diseases. Current vaccines have limited efficacy and can only protect against specific serotypes. Pneumococcal Surface Protein A (PspA) is an important vaccine candidate due to its role in evading the host immune system. Researchers have tested a delivery system based on the Bordetella pertussis adenylate cyclase toxin to induce immune responses against PspA in mice.
Meeting Abstract
Chemistry, Multidisciplinary
Marcia Carvalho de Abreu Fantini, Cristiano Luis Pinto Oliveira, Jose Luiz de Souza Lopes, Tereza da Silva Martins, Milena Apetito Akamatsu, Aryene Goes Trezena, Milene Tino de Franco, Viviane Fongaro Botosso, Osvaldo Augusto Brazil Esteves Sant Anna, Nikolay Kardjilov, Martin Kjaerulf Rasmussen, Heloisa Nunes Bordallo
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2021)
