Journal
VACCINE
Volume 27, Issue 21, Pages 2741-2745Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.03.013
Keywords
Toxoplasma gondii; TgSAG1; Pseudorabies virus; DNA vaccine; Prime-boost regime; Mice
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Funding
- National Special Research Programs for Non-Profit Trades (Agriculture) [200803017]
- Program for Chargjiang Scholars and Innovative Research Team in University [IRT0723]
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An effective vaccine of animals call block transmission of Toxoplasma gondii to humans. In this study, mice have been protected against lethal T gondii challenge by a prime-boost vaccination strategy using DNA vaccine pVAX/TgSAG1 and recombinant pseudorabies Virus rPRV/TgSAG1, both expressing the major immunodominant surface antigen of T. gondii (TgSAG1). High levels of splenocyte proliferative responses and significant levels of IFN-gamma resulted, with strong cytotoxic T lymphocyte (CTL) responses in vitro. After lethal challenge, prime-boost vaccinated mice showed all increased survival time (15.4 +/- 5.0 days) and a 40% survival rate compared with controls who all died within 11 days of challenge. Results of the present Study indicated that this novel immunization strategy is useful ill enhancing immune protection in mice against lethal 7: gondii infection, which would provide foundation for the development of effective vaccines against T. gondii. (C) 2009 Elsevier Ltd. All rights reserved.
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