Journal
VACCINE
Volume 27, Issue 43, Pages 5982-5988Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.07.071
Keywords
Rice-based vaccine; MucoRice; Oral vaccine; Cholera; Cold-chain-free; Mutant cholera toxin
Categories
Funding
- Research and Development Program for New Bio-industry Initiatives of the Bio-oriented Technology Research Advancement Institution
- Japan Society for the Promotion of Science
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Ministry and Health and Labour of Japan
- Ministry of Economy, Trade and Industry
- Center of Education and Research for the Advanced Genome-based Medicine for Personalized Medicine and the Control of Worldwide Infectious Diseases
- Academic Frontier
- Ministry of Agriculture, Forestry and Fisheries
- NIH [DE12242, AG025873]
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Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system. (C) 2009 Elsevier Ltd. All rights reserved.
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