Journal
VACCINE
Volume 26, Issue 27-28, Pages 3377-3382Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.04.062
Keywords
monoclonal antibodies; foot-and-mouth disease virus; double antibody sandwich ELISA
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A double antibody sandwich ELISA for quantification of foot-and-mouth disease (FMD) whole virus particle was developed using two monoclonal antibodies (mAbs) against FIVID virus (FMDV) serotype O and A. The antibody binding epitopes were characterized. The two mAbs reacted with all of the tested FMDV strains. The use of these two mAbs in the sandwich ELISA will allow the quantification of FMD whole virus particles during vaccine manufacture. (c) 2008 Elsevier Ltd. All rights reserved.
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