Article
Pharmacology & Pharmacy
Go Anan, Takuo Hirose, Daisuke Kikuchi, Chika Takahashi, Akari Endo, Hiroki Ito, Shigemitsu Sato, Shingo Nakayama, Hideaki Hashimoto, Katsuya Ishiyama, Tomoyoshi Kimura, Kazuhiro Takahashi, Makoto Sato, Takefumi Mori
Summary: Research findings suggest that SGLT2 inhibitors can effectively prevent the formation of renal stones, providing a new direction for the treatment of nephrolithiasis through large-scale epidemiological data, animal models, and cell culture experiments.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Chemistry, Physical
Ricardo D. Sosa, Xi Geng, Jacinta C. Conrad, Michael A. Reynolds, Jeffrey D. Rimer
Summary: Through the study, it was found that hydroxycitrate (a naturally derived small molecule) can effectively inhibit barite nucleation and fully suppress growth by forming a disordered layer on barite surfaces. The rate of barite growth could not be recovered over 12 hours after removal of the modifier, indicating that growth was sustainably (and irreversibly) altered by exposure to hydroxycitrate. Thus, hydroxycitrate is a barite crystallization modifier capable of suppressing nucleation and irreversibly inhibiting surface growth.
CHEMISTRY OF MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Daniel Turudic, Anja Tea Golubic, Mila Lovric, Marko Bilic, Danko Milosevic
Summary: The research found different risks and important variables in urinary stone development between prepubertal and pubertal/postpubertal children, with citrate and glycosaminoglycans increasingly important in children. J48 classifier confirmed the importance of certain variables for distinguishing between children with urolithiasis and healthy children of the same age and gender.
Article
Cell Biology
Yu Lan, Wei Zhu, Xiaolu Duan, Tuo Deng, Shujue Li, Yang Liu, Zhou Yang, Yaoan Wen, Lianming Luo, Shankun Zhao, Jiamin Wang, Zhijian Zhao, Wenqi Wu, Guohua Zeng
Summary: The study identified a novel role of glycine in regulating kidney calcium oxalate crystal formation, showing that glycine can attenuate CaOx crystal deposition by decreasing urine oxalate and increasing urine citrate levels. The mechanism involves downregulating Slc26a6 expression to decrease urine oxalate and inhibiting Nadc1 expression to increase urine citrate, with additional regulation of protein expression by miRNA-411-3p. These findings provide a potential target for the treatment of kidney calcium oxalate stones.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Urology & Nephrology
Nancy S. Krieger, John Asplin, Ignacio Granja, Luojing Chen, Daiana Spataru, Tong Tong Wu, Marc Grynpas, David A. Bushinsky
Summary: In this study, the effects of potassium citrate and chlorthalidone on calcium oxalate stone formation and bone quality were investigated using a genetic hypercalciuric stone-forming rat model. The results showed that potassium citrate plus chlorthalidone combination was more effective in reducing stone formation, while chlorthalidone alone improved bone quality.
KIDNEY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Silvia Berto, Martino Marangella, Concetta De Stefano, Demetrio Milea, Pier Giuseppe Daniele
Summary: New complexes such as [Cacit](4-) and [Ca2H2(PO4)(2)] significantly lower beta values in patients with nephrolithiasis. Investigation of the Ca-phosphate-citrate aqueous system through potentiometric titrations revealed that the [Ca(PO4)cit](4-) species is only noticeable at pH > 8.5.
Review
Health Care Sciences & Services
Kyriaki Stamatelou, David S. Goldfarb
Summary: In the past two decades, there have been no major breakthroughs in the understanding and therapy of kidney stones, which remains a challenging disease for patients, physicians, and healthcare systems. Epidemiological studies play a crucial role in identifying global changes in the prevalence and burden of kidney stones and modifiable risk factors. The expanding knowledge of kidney stone epidemiology significantly enhances the modern management of the disease.
Article
Chemistry, Multidisciplinary
Michael Emmanuel, Paszkal Papp, Gabor Schuszter, Agota Deak, Laszlo Janovak, Agota Toth, Dezso Horvath
Summary: This article provides insights into the kinetics of lithium phosphate precipitation in a supersaturated solution through experimental data analysis and reaction kinetic model construction. It explains the mechanism of nucleation in detail and compares it with classical nucleation theory.
Article
Chemistry, Multidisciplinary
Hao Lu, David Yuen Wah Ng, Ingo Lieberwirth, Tobias Weidner, Mischa Bonn
Summary: This study elucidates the mechanism of the acidic osteopontin (OPN) in inhibiting the formation of kidney stones by interacting with calcium ions and calcium oxalate. The findings provide important insights into understanding the development of kidney stones.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Urology & Nephrology
Polycronis P. Akouris, John A. Chmiel, Gerrit A. Stuivenberg, Wongsakorn Kiattiburut, Jennifer Bjazevic, Hassan Razvi, Bernd Grohe, Harvey A. Goldberg, Jeremy P. Burton, Kait F. Al
Summary: Kidney stone disease affects a large number of individuals and imposes a significant economic burden on healthcare systems globally. This study demonstrates for the first time that a synthetic OPN phosphopeptide can effectively reduce stone burden in vivo, suggesting that OPN supplementation may be a promising therapeutic approach for the management of urolithiasis.
Article
Multidisciplinary Sciences
Shyam Sundar Choudhary, Padma Nibash Panigrahi, Sujoy K. Dhara, Monalisa Sahoo, Ananya Dan, Neeraj Thakur, Aron Jacob, Sahadeb Dey
Summary: Cucumis callosus fruit is traditionally used in north-west India to treat urinary ailments. This study validates the ethnobotanical use of Cucumis fruit in urolithiasis by evaluating its anti-urolithiatic potential in rats with induced hyperoxaluria. The results demonstrate the curative efficacy of Cucumis fruit extract in urolithiasis and suggest beneficial effects on renal function.
Article
Biochemistry & Molecular Biology
Chiharuko Ushimoto, Shigeru Sugiki, Kenshirou Kunii, Shinya Inoue, Eriko Kuroda, Ryoko Akai, Takao Iwawaki, Katsuhito Miyazawa
Summary: Oxidative stress is a significant risk factor for calcium oxalate nephrolithiasis. This study investigates the association between oxidative stress response and stone formation through analyzing the response during different phases and observing the effect on crystal formation in mouse models. The findings suggest that the oxidative stress response via Keap1-Nrf2 pathway may contribute to crystal formation and could be a potential target for preventing and treating nephrolithiasis.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Engineering, Chemical
Sevgi Polat, Huseyin Burak Eral
Summary: Recent studies have shown that hyaluronic acid (HA) can influence the morphology and phase of calcium oxalate (CaOx) crystals, promoting the transformation of CaOx crystals in aqueous solution. Particularly, in artificial urine media, the addition of HA resulted in the formation of calcium oxalate dihydrate (COD) crystals with tetragonal bipyramidal morphology.
ADVANCED POWDER TECHNOLOGY
(2021)
Article
Urology & Nephrology
Vahid Solak, Mehmet Ilker Gokce, Onder Yaman
Summary: This study found that potassium citrate significantly reduced calcium excretion and increased citrate excretion in patients with calcium oxalate stones and hypercalciuria. At the 12-month follow-up, the K-CIT group had a lower rate of stone recurrence compared to the HCT group.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2021)
Article
Urology & Nephrology
Raymond Khargi, Ryan M. Blake, Alan J. Yaghoubian, Caroline Canning, Alexander Fang, Christopher Connors, Blair Gallante, Anna Ricapito, Johnathan A. Khusid, William M. Atallah, Mantu Gupta
Summary: This study aims to identify unique urinary metabolic abnormality profiles in octogenarians with calcium oxalate kidney stones, in order to guide empiric stone prevention therapy more precisely in this population.
WORLD JOURNAL OF UROLOGY
(2023)
Article
Nanoscience & Nanotechnology
Julie Schmokel, Anders Voldum, Georgia Tsakiridou, Matthias Kuhlmann, Jason Cameron, Esben S. Sorensen, Jesper Wengel, Kenneth A. Howard
Article
Gastroenterology & Hepatology
Signe Bruun, Lotte Neergaard Jacobsen, Xiaolei Ze, Steffen Husby, Hiroshi M. Ueno, Keisuke Nojiri, Shunjiro Kobayashi, Jungil Kwon, Xihong Liu, Shuyuan Yan, Jiyeon Yang, Gitte Zachariassen, Liang Chen, Wei Zhou, Brian Christensen, Esben S. Sorensen
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2018)
Article
Biochemistry & Molecular Biology
Gitte N. Schytte, Brian Christensen, Ida Bregenov, Katarzyna Kjoge, Carsten Scavenius, Steen V. Petersen, Jan J. Enghild, Esben S. Sorensen
JOURNAL OF CELLULAR BIOCHEMISTRY
(2020)
Article
Agriculture, Dairy & Animal Science
B. Christensen, N. J. Karlsen, S. D. S. Jorgensen, L. N. Jacobsen, M. S. Ostenfeld, S. Petersen, A. Mullertz, E. S. Sorensen
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Gitte N. Schytte, Brian Christensen, Ida Bregenov, Esben S. Sorensen
BIOSCIENCE REPORTS
(2020)
Article
Nutrition & Dietetics
Brian Christensen, Andrea E. Toth, Simone S. E. Nielsen, Carsten Scavenius, Steen V. Petersen, Jan J. Enghild, Jan T. Rasmussen, Morten S. Nielsen, Esben S. Sorensen
Article
Biology
Stacyann Bailey, Grazyna E. Sroga, Betty Hoac, Orestis L. Katsamenis, Zehai Wang, Nikolaos Bouropoulos, Marc D. McKee, Esben S. Sorensen, Philipp J. Thurner, Deepak Vashishth
Article
Agriculture, Dairy & Animal Science
Brian Christensen, Elias D. Zachariae, Nina A. Poulsen, Albert J. Buitenhuis, Lotte B. Larsen, Esben S. Sorensen
Summary: This study aimed to determine the content of bioactive protein osteopontin (OPN) in bovine milk and developed an ELISA method to measure OPN concentration in milk from Danish Holstein cows. The study found that OPN levels in milk have a low heritability and significant individual variation, with levels increasing with days in milk and decreasing with parity.
JOURNAL OF DAIRY RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Andreas B. Bertelsen, Celeste Menuet Hackney, Carolyn N. Bayer, Lau D. Kjelgaard, Maja Rennig, Brian Christensen, Esben Skipper Sorensen, Helena Safavi-Hemami, Tune Wulff, Lars Ellgaard, Morten H. H. Norholm
Summary: Secreted proteins and peptides have great potential as therapeutics and enzyme catalysts due to their high stability, which is maintained by disulphide bonds. However, limited availability of synthetic biology tools and heterologous production systems hampers the discovery and applications of these proteins. In this study, the design of DisCoTune plasmids in the Escherichia coli T7 protein production system successfully enhanced the yield and activity of an industrial protease and a conotoxin, demonstrating the promising potential of this technology for biotechnological applications.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mathilde Frost Kristensen, Esben Skipper Sorensen, Yumi Chokyu Del Rey, Sebastian Schlafer
Summary: This study investigated the ability of osteopontin (OPN) to prevent the adhesion of dental biofilm-forming bacteria. OPN was found to be the most effective protein in reducing bacterial adhesion. Bioactive milk proteins show potential to delay harmful biofilm formation on teeth and hence the onset of biofilm-related oral disease.
Article
Psychology, Biological
Muhammed Alparslan Gokhan, Esben Skipper Sorensoen, Lene Baad-Hansen
Summary: This study aimed to assess the role of milk proteins in reducing capsaicin-induced oral burning sensation. Participants dipped their tongues in capsaicin gel and rinsed their mouth with different solutions including milk proteins. The results showed that short-term rinsing with milk proteins did not significantly alter capsaicin-induced oral burning sensation, unpleasantness, somatosensory changes, or tongue temperature compared with control.
PHYSIOLOGY & BEHAVIOR
(2023)
Review
Nutrition & Dietetics
Esben S. Sorensen, Brian Christensen
Summary: Osteopontin (OPN) is a multifunctional protein that is expressed in various cell types and found in most tissues and physiological secretions. It plays a role in immune system regulation, biomineralization, tissue development, bacterial interactions, and more. OPN is highly concentrated in milk and is believed to have developmental and physiological effects on infants. This article reviews and compares existing knowledge on the structure, function, and health implications of milk OPN.
Article
Nutrition & Dietetics
Brian Christensen, Albert J. Buitenhuis, Lotte N. Jacobsen, Marie S. Ostenfeld, Esben S. Sorensen
Summary: Osteopontin (OPN) is abundant in human milk and has a lower concentration in bovine milk. Both human and bovine milk OPN resist digestion and have beneficial effects on intestinal development. This study compared the effect of digested human and bovine milk OPN on gene expression in Caco-2 cells and found that both OPNs regulated the expression of genes related to biological processes such as the ubiquitin system, DNA binding, transcription, and transcription control pathways.
Article
Biochemistry & Molecular Biology
Brian Christensen, Nanna R. Nielsen, Marie R. Sorensen, Lotte N. Jacobsen, Marie S. Ostenfeld, Esben S. Sorensen
Summary: The aim of this study was to investigate the interaction between Osteopontin (OPN) and intestinal cells, as well as its transport across models of the intestinal barrier. The results showed that OPN binds to intestinal cells but is not internalized. It was also found that OPN crosses the barrier models through the transcytosis pathway, and this process is time-, temperature-, and energy-dependent.
Article
Endocrinology & Metabolism
Brian Christensen, Gitte N. Schytte, Carsten Scavenius, Jan J. Enghild, Marc D. Mckee, Esben S. Sorensen