Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Chemistry, Analytical
Iene Rutten, Devin Daems, Karen Leirs, Jeroen Lammertyn
Summary: Evalution(TM) is a novel microfluidic platform that combines encoded microparticles and channel-based microfluidics to enable fast, sensitive, and simultaneous detection of multiple disease-related biomarkers.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Chemistry, Analytical
Linlin Zhuang, Jianbo Yang, Chunlei Song, Li Sun, Bin Zhao, Qiuping Shen, Xiyan Ren, Hongjing Shi, Yu Zhang, Mengling Zhu
Summary: An accurate and practical graphene oxide-based method, called GO-ASEA, was developed for rapid and highly sensitive detection of African swine fever virus (ASFV). This novel assay showed excellent specificity and selectivity, with a low detection limit, and demonstrated good clinical applicability. The GO-ASEA method has the potential to serve as a robust tool in epidemic prevention and point-of-care diagnosis.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Dalton J. Nelson, Mindy Leelawong, Megan E. Pask, C. William Wester, Muktar H. Aliyu, Frederick R. Haselton
Summary: This report presents the first step towards a highly specific SNP detection method using magnetic bead processing and PCR detection, which is suitable for resource-limited clinics and allows for more effective clinical management of drug resistance mutations.
ANALYTICAL CHEMISTRY
(2022)
Article
Pediatrics
Hyun-Woo Lee, Seung-Beom Han, Jung-Woo Rhim
Summary: This study aimed to determine the subjects for bacterial multiplex polymerase chain reaction (mPCR) testing and to interpret the mPCR test results based on patients' clinical symptoms and diagnoses. The study found that positive mPCR test results were significantly associated with older age, diagnosis of acute gastroenteritis, presence of hematochezia, and absence of cough. Additionally, the identification of invasive pathogens in patients with positive mPCR test results was significantly associated with the diagnosis of acute gastroenteritis, presence of fever and diarrhea, and higher C-reactive protein levels. Therefore, selective bacterial mPCR testing should be performed based on the patients' clinical symptoms and diagnoses.
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Agriculture, Dairy & Animal Science
Qianying Li, Guoyang Xie, Yutong Wang, Zoraida P. Aguilar, Hengyi Xu
Summary: A new technique using vancomycin-modified poly-L-lysine magnetic beads was developed for the isolation of gram-positive bacteria, with efficient genotyping and detection achieved through mPCR assay. The results showed excellent capture and isolation efficiency for Bacillus cereus, indicating the potential for universal application in enrichment and genotyping of gram-positive bacteria.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sunao Sugita, Ayumi Hono, Shoko Fujino, Yoko Futatsugi, Yuta Yunomae, Norio Shimizu, Masayo Takahashi
Summary: This study tested a PCR method for detecting mycoplasma contamination in laboratory cultures, showing high specificity and ability to detect contamination at higher concentrations with 100% probability. Commercially available cell lines may carry infection risks.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Nuli Xie, Huimin Wang, Jing Li, Kemin Wang, Jin Huang
Summary: A dual amplification strategy combining bio-barcode technique and auto-cycling primer extension is proposed for the sensitive detection of miRNA targets in complex biological samples. The strategy shows good sensitivity, with a detection limit of 50 fM, and can effectively distinguish different miRNAs, providing a new approach for the sensitive detection of nucleic acid targets.
CHEMICAL COMMUNICATIONS
(2022)
Article
Food Science & Technology
Sharmin Sultana, Azrina Azlan, Mohd Nasir Mohd Desa, Nor Ainy Mahyudin
Summary: The genetic information in DNA is crucial, and the identification of selective DNA sequences is essential in biological studies. Various authentication techniques, including PCR assays, nanotechnology-based biosensors, and microarray devices, have evolved to trace and detect different DNA targets. While detecting single DNA is relatively straightforward, the innovation of multiple platforms is required to identify hundreds or even thousands of DNA targets in a cost-effective and time-saving manner. This review provides an up-to-date documentary of DNA techniques for species identification in the food chain, discussing current knowledge, limitations, opportunities, and hurdles.
Article
Biotechnology & Applied Microbiology
Chunyun Zhang, Yuanyuan Wang, Yihan Wang, Fuguo Liu, Guofu Chen
Summary: This study developed a MPCR assay for the detection of harmful microalgae in the marine aquaculture environment, which showed high specificity and stability. The MPCR assay can simultaneously detect multiple common harmful microalgae and has a low detection limit even at low genomic DNA concentrations.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Multidisciplinary Sciences
Jackson Alves da Silva Queiroz, Tarcio Peixoto Roca, Rutilene Barbosa Souza, Luiz Fellype Alves de Souza, Ana Maisa Passos-Silva, Andre Luiz Ferreira da Silva, Eugenia de Castro e Silva, Lourdes Maria Pinheiro Borzacov, Rita de Cassia Pontello Rampazzo, Soraya dos Santos Pereira, Thor Oliveira Dantas, Janaina Mazaro, Livia Melo Villar, Juan Miguel Villalobos Salcedo, Daniel Archimedes da Matta, Deusilene Vieira
Summary: This study developed a one-step RT-qPCR method for detecting hepatitis Delta virus, which was proven to be highly efficient and reproducible. The method can be used to monitor the disease progression and assess the effectiveness of treatment in hepatitis Delta patients.
SCIENTIFIC REPORTS
(2023)
Article
Medical Laboratory Technology
Zhikang Yu, Heming Wu, Qingyan Huang, Zhixiong Zhong
Summary: The development of a rapid, specific, and sensitive quantitative PCR method to detect Marburg virus and Ebola virus simultaneously is of great importance for epidemic monitoring and prevention and control of infectious diseases. The TaqMan probe-based multiplex fluorescence quantitative PCR assays showed high sensitivity and specificity in detecting both viruses simultaneously.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Immunology
Se Chang Oh, Soo Min Park, Jian Hur, Eun Young Choi, Hyun Jung Jin, Yu Kyung Kim, Jong Ho Lee, Ji Young Ahn, Jae Min Lee
Summary: The study investigated the effectiveness of mPCR for early diagnosis of pertussis, revealing that most patients were diagnosed within two weeks from symptom onset and required hospitalization and antibiotic treatment.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Oncology
Tamy Taianne Suehiro, Gabrielle Marconi Zago Ferreira Damke, Edilson Damke, Paloma Luana Rodrigues de Azevedo Ramos, Marcela de Andrade Pereira Silva, Sandra Marisa Pelloso, Warner K. Huh, Ricardo Argemiro Fonseca Franco, Vania Ramos Sela da Silva, Isabel Cristina Scarinci, Marcia Edilaine Lopes Consolaro
INFECTIOUS AGENTS AND CANCER
(2020)
Article
Medicine, Research & Experimental
Gabrielle Marconi Zago Ferreira Damke, Edilson Damke, Patricia de Souza Bonfim-Mendonca, Bianca Altrao Ratti, Lyvia Eloiza de Freitas Meirelles, Vania Ramos Sela da Silva, Renato Sonchini Goncalves, Gabriel Batista Cesar, Sueli de Oliveira Silva, Wilker Caetan, Noboru Hioka, Raquel Pantarotto Souza, Marcia Edilaine Lopes Consolaro
Article
Pharmacology & Pharmacy
Silvana Sandri, Luis R. M. Watanabe, Erica Aparecida de Oliveira, Fernanda Faiao-Flores, Silene Migliorini, Manoela Tiago, Aloisio Felipe-Silva, Vinicius de Lima Vazquez, Paola da Costa Souza, Marcia Edilaine Lopes Consolaro, Ana Campa, Silvya Stuchi Maria-Engler
PHARMACOLOGICAL RESEARCH
(2020)
Review
Public, Environmental & Occupational Health
Fabricio Morelli, Lyvia E. de F. Meirelles, Maria V. F. de Souza, Natalia L. Mari, Cristiane S. S. Mesquita, Camila B. Dartibale, Gabrielle M. Z. F. Damke, Edilson Damke, Vania R. S. da Silva, Raquel P. Souza, Marcia E. L. Consolaro
Summary: SARS-CoV-2, the virus responsible for COVID-19, has been detected in multiple human tissues where the receptor ACE2 is expressed, indicating potential risks to the reproductive tract. While the virus has been found in bodily fluids like blood, urine, semen, and vaginal secretions, limited information is available on infection in the male and nonpregnant female reproductive tracts, necessitating further research to understand sexual transmission and fertility issues.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Pharmacology & Pharmacy
Erica Aparecida de Oliveira, Jagat Chauhan, Julia Rezende da Silva, Larissa Anastacio da Costa Carvalho, Diogo Dias, Danielle Goncalves de Carvalho, Luis Roberto Masao Watanabe, Vito W. Rebecca, Gordon Mills, Yiling Lu, Aloisio Souza Felipe da Silva, Marcia Edilaine Lopes Consolaro, Meenhard Herlyn, Patricia A. Possik, Colin R. Goding, Silvya Stuchi Maria-Engler
Summary: TOP1 expression is correlated with invasive gene signature in metastatic melanoma, with highest expression in metastatic cells and lowest expression in BRAFi- and BRAFi/MEKi-resistant cells. These results suggest a potential role of TOP1 in melanoma progression and resistance.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Lucimara R. Carobeli, Lyvia E. de F. Meirelles, Gabrielle M. Z. F. Damke, Edilson Damke, Maria V. F. de Souza, Natalia L. Mari, Kayane H. Mashiba, Cristiane S. Shinobu-Mesquita, Raquel P. Souza, Vania R. S. da Silva, Renato S. Goncalves, Wilker Caetano, Marcia E. L. Consolaro
Summary: The review provides an overview of the pathophysiology of cervical cancer and recent developments in PDT therapy, emphasizing the potential of phthalocyanines as a pharmaceutical agent for anti-cervical cancer therapy.
Article
Pharmacology & Pharmacy
Fernanda Belincanta Borghi-Pangoni, Jessica Bassi da Silva, Rafaela Said dos Santos, Ana Paula Trevisan, Francyelle Carolyne de Castro Hott, Marcelly Chue Goncalves, Renata Katsuko Takayama Kobayashi, Maria Vitoria Felipe de Souza, Marcia Edilaine Lopes Consolaro, Lidiane Vizioli de Castro-Hoshino, Mauro Luciano Baesso, Marcos Luciano Bruschi
Summary: This study describes the development of a bioadhesive thermoresponsive platform for propolis skin delivery. Results showed that the Polox/HPMC/PES system, with the addition of cellulose derivatives and poloxamer 407, exhibited reduced cytotoxicity, modified propolis release, enhanced skin permeation, and activity against P. acnes.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2022)
Article
Obstetrics & Gynecology
Lais de S. Marques, Vanessa G. A. Violin, Lyvia E. de F. Meireles, Maria V. F. de Souza, Natalia L. Mari, Gabrielle M. Z. F. Damke, Edilson Damke, Monalisa W. Pereira, Cristiane S. S. Mesquita, Vania R. S. da Silva, Marcia E. L. Consolaro
Summary: Synopsis kin17 high expression is a novel potential biomarker for high-grade CIN.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2023)
Article
Multidisciplinary Sciences
Fabiani de Morais Batista, Marco Antonio Moreira Puga, Patricia Vieira da Silva, Roberto Oliveira, Paulo Cesar Pereira dos Santos, Bruna Oliveira da Silva, Mariana Bento Tatara, Daniel Henrique Tsuha, Maria Aparecida dos Santos Pires, Crhistinne Cavalheiro Maymone Goncalves, Romulo Pessoa E. Silva, Nathalia Tavares Ferreira, Amanda Pinheiro de Barros Albuquerque, Giselle da Silva Duarte, Marcia Edilaine Lopes Consolaro, Fabio Juliano Negrao, Idalina Cristina Ferrari, Luciano Pamplona de Goes Cavalcanti, Karen Soares Trinta, Guilherme S. Ribeiro, Moacyr Jesus Barreto de Melo Rego, Rosemary J. Boyton, Andre Machado Siqueira, Daniel M. Altmann, Julio Croda
Summary: This study analyzed the immune response of patients with SARS-CoV-2 infection and identified biomarkers related to disease severity. Some angiogenesis markers were more frequently elevated in severe cases compared to other groups. In terms of cardiovascular diseases, D-dimer, GDF-15, and sICAM-1 were higher in the severe cases. Significant differences in cytokines and chemokines levels were also observed according to the clinical course.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Camila B. Dartibale, Gabriela de C. Prado, Lucimara R. Carobeli, Lyvia E. de F. Meirelles, Gabrielle M. Z. F. Damke, Edilson Damke, Fabricio Morelli, Raquel P. Souza, Vania R. S. Grp Prevent Yorself, Vania R. S. da Silva, Marcia E. L. Consolaro
Summary: Cervical cancer is a major health issue in Latin America and Caribbean, and many countries have not been successful in implementing screening programs. Self-sampling, as an alternative approach, has shown promise. This systematic review summarizes recent HPV self-sampling approaches in the region and highlights the potential of this strategy in overcoming barriers to screening and increasing attendance among underscreened women.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lyvia Eloiza de Freitas Meirelles, Maria Vitoria Felipe de Souza, Lucimara Rodrigues Carobeli, Fabricio Morelli, Natalia Lourenco Mari, Edilson Damke, Cristiane Suemi Shinobu Mesquita, Jorge Juarez Vieira Teixeira, Marcia Edilaine Lopes Consolaro, Vania Ramos Sela da Silva
Summary: A systematic review investigated the in vitro effects of combining conventional drugs and bioactive compounds derived from cinnamic acid in breast cancer treatment. The combination improved the overall effectiveness of conventional drugs, increasing cytotoxicity, antimigratory effects, and reversing resistance. Combining conventional drugs with bioactive compounds derived from cinnamic acid could provide new treatment options for different types of breast cancer.
Article
Infectious Diseases
Giselle da Silva Duarte, Alexandra Jones, Luciano Pamplona de Goes Cavalcanti, Moacyr Jesus Barreto de Melo Rego, Guilherme Ribeiro, Rosemary Boyton, Dhelio Batista Pereira, Julio Henrique Rosa Croda, Fabio Trindade Maranhao Costa, Angela Pinto Duarte, Marcia Edilaine Lopes Consolaro, Rodrigo Guerino Stabeli, Fabio Juliano Negrao, Jose Luiz Proenca-Modena, Juan Miguel Villalobos-Salcedo, Geraldo da Rocha Castelar Pinheiro, Amanda Pinheiro de Barros Albuquerque, Francisca Kalline de Almeida Barreto, Jose Moreira, Idalina Cristina Ferrari, Patricia Martinez Evora, Vania Ramos Sela da Silva, Marcus Vinicius Guimaraes Lacerda, Daniel Altmann, REPLICK Network, Andre M. Siqueira
Summary: This study is the largest evaluation of the long-term sequelae of individuals infected with CHIKV in the Brazilian population, focusing on musculoskeletal manifestations, mental health, quality of life, and chronic pain. The aim of this study is to better understand the disease burden and costs associated with CHIKV infection and to inform therapeutic guidelines.
BMC INFECTIOUS DISEASES
(2023)
Article
Pharmacology & Pharmacy
Kayane Harumi Mashiba, Lucimara Rofrigues Carobeli, Maria Vitoria Felipe de Souza, Lyvia Eloiza de Freitas Meirelles, Natalia Lourenco Mari, Gabriel Batista Cesar, Renato Sonchini Goncalves, Wilker Caetano, Edilson Damke, Vania Ramos Sela da Silva, Gabrielle Marconi Zago Ferreira Damke, Marcia Edilaine Lopes Consolaro
Summary: Cervical cancer is a leading cause of death among women, and current treatment options for endocervical adenocarcinoma (ECA) are ineffective. This study evaluated the effectiveness of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) on human cell lines derived from invasive cervical adenocarcinoma (HeLa) compared to a human epithelial cell line (HaCaT). The results showed that F127/HYP had a dose-dependent and selective phototoxic effect on HeLa cells, caused cell death through necrosis and reactive oxygen species (ROS) production, and reduced long-term proliferation and migration capacity of HeLa cells. These findings suggest that F127/HYP micelles may be a promising approach for specifically treating ECA using PDT.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Infectious Diseases
Tamy Taianne Suehiro, Fabricia Gimenes, Raquel Pantarotto Souza, Sergio Ken Iti Taura, Rita Cristina Cardoso Cestari, Mary Mayumi Taguti Irie, Cinthia Gandolfi Boer, Marcia Edilaine Lopes Consolaro, Vania Ramos Sela da Silva
Summary: The study investigated the molecular prevalence of HPV and other common STIs in asymptomatic women at a Brazilian university, finding a high prevalence of HPV and common multiple HPV infections. There was at least one STI detected in most tested women, with Chlamydia trachomatis being the most prevalent, and low rates of cytological abnormalities were observed.
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO
(2021)
Review
Biochemistry & Molecular Biology
David Livingstone Alves Figueiredo, Joao Paulo Bianchi Ximenez, Fabio Rodrigues Ferreira Seiva, Carolina Panis, Rafael dos Santos Bezerra, Adriano Ferrasa, Alessandra Lourenco Cecchini, Alexandra Ivo de Medeiros, Ana Marisa Fusco Almeida, Anelisa Ramao, Angelica Beate Winter Boldt, Carla Fredrichsen Moya, Chung Man Chin, Daniel de Paula, Daniel Rech, Daniela Fiori Gradia, Danielle Malheiros, Danielle Venturini, Eliandro Reis Tavares, Emerson Carraro, Enilze Maria de Souza Fonseca Ribeiro, Evani Marques Pereira, Felipe Francisco Tuon, Franciele Ani Caovilla Follador, Glaura Scantamburlo Alves Fernandes, Helito Volpato, Ilce Mara de Syllos Colus, Jaqueline Carvalho de Oliveira, Jean Henrique da Silva Rodrigues, Jean Leandro dos Santos, Jeane Eliete Laguila Visentainer, Juliana Cristina Brandi, Juliana Mara Serpeloni, Juliana Sartori Bonini, Karen Brajao de Oliveira, Karine Fiorentin, Leia Carolina Lucio, Ligia Carla Faccin-Galhardi, Lirane Elize Defante Ferreto, Lucy Megumi Yamauchi Lioni, Marcia Edilaine Lopes Consolaro, Marcelo Ricardo Vicari, Marcos Abdo Arbex, Marcos Pileggi, Maria Angelica Ehara Watanabe, Maria Antonia Ramos Costa, Maria Jose S. Mendes Giannini, Marla Karine Amarante, Najeh Maissar Khalil, Quirino Alves de Lima Neto, Roberto H. Herai, Roberta Losi Guembarovski, Rogerio N. Shinsato, Rubiana Mara Mainardes, Silvana Giuliatti, Sueli Fumie Yamada-Ogatta, Viviane Knuppel de Quadros Gerber, Wander Rogerio Pavanelli, Weber Claudio da Silva, Maria Luiza Petzl-Erler, Valeria Valente, Christiane Pienna Soares, Luciane Regina Cavalli, Wilson Araujo Silva Jr
Summary: COVID-19, caused by SARS-CoV-2, is the largest pandemic in modern history with high infection rates and mortality. Efforts are being made to develop vaccines, new drugs, and repurpose existing ones to control the pandemic. The therapeutic strategies aim to interfere with viral replication and patient immunopathology.
GENETICS AND MOLECULAR BIOLOGY
(2021)