Article
Immunology
Youliang Ren, Thomas Xue, Joshua Rainbolt, Karen L. de Mesy Bentley, Chad A. Galloway, Yuting Liu, Philip Cherian, Jeffrey Neighbors, Marloes I. Hofstee, Frank H. Ebetino, Thomas Fintan Moriarty, Shuting Sun, Edward M. Schwarz, Chao Xie
Summary: This study demonstrates the in vivo efficacy of bone-targeted BCA in treating S. aureus infection of bone, showing potential superiority over standard-of-care antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Werasak Sutipornpalangkul, Kohei Nishitani, Edward M. Schwarz
Summary: This study investigated the natural history of S. aureus in in vitro biofilm formation in human plasma media using a flow chamber system, revealing strain-specific phenotypes and the potential use to evaluate anti-microbial surfaces. Bioluminescent S. aureus strains were used to understand bacterial growth and biofilm formation on orthopaedic materials, demonstrating the differences in biofilm formation between bacterial strains and implant materials.
Article
Food Science & Technology
Amal S. Albedwawi, Reem Al Sakkaf, Tareq M. Osaili, Ahmed Yusuf, Amin Olaimat, Shao-Quan Liu, Giovanni Palmisano, Nagendra P. Shah, Mutamed M. Ayyash
Summary: This paper investigated the ability of lactic acid bacteria (LAB) and Bifidobacterium to remove acrylamide (AA) and examined the mechanism(s) of the AA removal using various microscopic techniques. The results showed that Bifidobacterium breve and Lactiplantibacillus plantarum exhibited higher acrylamide reduction rates. The study also found that both strains could tolerate low pH levels and remove acrylamide under in vitro gastrointestinal conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Cell Biology
Xavier Heiligenstein, Miriam S. Lucas
Summary: Sample preparation is a critical step for high throughput CLEM and requires balancing the requirements of fluorescence and electron microscopy. Resin embedding is a commonly used method for electron microscopy, but contrasting agents can impair fluorescence. The discovery of preserving fluorescence in resin-embedded specimens after mild heavy metal staining has greatly advanced CLEM. This article critically reflects upon resin embedding and discusses existing workflows utilizing in-resin fluorescence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Komal Tilwani, Abhishek Patel, Hirak Parikh, Janki Thakker, Gayatri Dave
Summary: This study explores the health benefits of Yarrow tea and evaluates its antiviral and anti-inflammatory properties. The results suggest that the constituent Luteolin in Yarrow tea may exhibit anti-inflammatory actions by inhibiting Cyclooxygenase II, and Yarrow exhibits virucidal activity against SARS-nCoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Cell Biology
Pieter Baatsen, Sergio Gabarre, Katlijn Vints, Rosanne Wouters, Dorien Vandael, Rose Goodchild, Sebastian Munck, Natalia V. Gounko
Summary: In life science research, it is important to determine the location of molecules within cells or tissues, typically using genetically encoded fluorescent proteins and fluorescence affinity-labeling. To add an ultrastructural context, correlative light and electron microscopy (CLEM) imaging can be used, which preserves both fluorescent signals and provides high-resolution electron microscopy imaging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Anirudha Karati, Simanta Lahkar, Yixuan Hu, Ramkrishna Sahoo, Harish Kumar Adigilli, Neha Y. Hebalkar, Kolan Madhav Reddy, Joydip Joardar
Summary: In this study, a Cu-Mo-S mixed chalcogenide was synthesized using wet chemical synthesis. The compound exhibited nanocube-like morphology with a size range of 30-50 nm. Investigation on its phase formation and pseudocapacitive properties revealed a ternary phase that does not match with any reported structure. Extensive analysis using high-resolution transmission electron microscopy and energy-dispersive X-ray spectroscopy confirmed the physical structure and chemical composition of the compound.
ACS APPLIED NANO MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Andjelika Bjelajac, Ileana Florea, Mihai Zamfir, Sandrine Tusseau Nenez, Costel Sorin Cojocaru
Summary: In this study, vertically aligned multiwall carbon nanotubes (CNTs) films were used as support for ZnO/ZnS photocatalytic active nanostructures. The CNTs were synthesized using a hot-filament chemical vapor deposition (HfCVD) method. Point defects in the CNTs outer walls were created using a low pressure nonthermal water vapors diffusive plasma, and Zn nanoparticles were subsequently electrodeposited onto the CNTs. The samples were annealed to obtain ZnO, ZnS, and a mix phase of ZnO/ZnS. The absorption of ZnO was enhanced by S doping and involvement of CNTs, and the ZnO1-xSx@CNTs heteronanostructure showed the highest photocatalytic activity for methyl blue dye degradation among the studied samples.
Article
Multidisciplinary Sciences
Wei Ping Teoh, Xi Chen, Irina Laczkovich, Francis Alonzo
Summary: During infection, pathogenic microbes such as Staphylococcus aureus adapt to the host's nutritional environment by scavenging necessary nutrients, including lipoic acid, and enzymes required for metabolism. The ability to acquire lipoic acid and unsaturated fatty acids from the host environment is crucial for the pathogen's virulence in different infection sites. The findings emphasize the importance of host lipid assimilation for bacterial survival and suggest challenges in targeting bacterial lipogenesis as an antibacterial strategy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Materials Science, Multidisciplinary
S. Y. Li, S. Y. Duan, W. Q. Ming, C. L. Wu, J. H. Chen
Summary: The S-phase is an effective strengthening precipitate in aluminum alloys for aerospace applications. Previous studies have well revealed its precipitation mechanism in AlCuMg alloys. However, this study reports a different scenario of S-phase formation in an AlCuLiMg alloy. By using atomic-resolution imaging in transmission electron microscopy, it is discovered that this alloy can form a precipitate microstructure dominated by Li-containing Guinier-Preston-Bagaryatsky zones and previously unknown S-like phase precipitates. The formation of the S-like precipitate undergoes a structural phase evolution rather than a conventional phase transformation. This study shows that AlCuLiMg alloys can develop a distinct microstructure with S-like/S-phase and T-1-phase precipitates, without the commonly observed delta'/theta'/delta' composite precipitates.
Article
Biochemistry & Molecular Biology
Soumyajit Roy, Moumita Halder, Palla Ramprasad, Suman Dasgupta, Yashveer Singh, Durba Pal
Summary: This study discovered that oxidized pullulan (o-pullulan), a biological macromolecule, exhibits strong antibacterial and antibiofilm activities against Staphylococcus aureus, a Gram-positive bacteria implicated in wound infections. O-pullulan targets the cell wall-associated membrane protein SpA, altering membrane potential and inhibiting Ca2+-Mg2+-ATPase pumps. It also scavenges intracellular reactive oxygen species (ROS), is non-toxic to mammalian cells, and shows great promise for chronic wound management.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Tobias C. Kunz, Marcel Ruhling, Adriana Moldovan, Kerstin Paprotka, Vera Kozjak-Pavlovic, Thomas Rudel, Martin Fraunholz
Summary: Expansion Microscopy (ExM) is a novel tool that improves the resolution of fluorescence microscopy by physically expanding the sample in a hydrogel, allowing visualization of various pathogens, including Gram-positive bacteria. A series of enzymatic treatments were developed to achieve isotropic 4x expansion of the Gram-positive pathogen Staphylococcus aureus, demonstrating the technique's suitability for imaging bacteria at a spatial resolution of approximately 60 nm with conventional confocal laser scanning microscopy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Article
Infectious Diseases
Emmanuela Adjei-Sowah, Yue Peng, Jason Weeks, Jennifer H. Jonason, Karen L. de Mesy Bentley, Elysia Masters, Yugo Morita, Gowrishankar Muthukrishnan, Philip Cherian, X. Eric Hu, Charles E. McKenna, Frank H. Ebetino, Shuting Sun, Edward M. Schwarz, Chao Xie
Summary: This study proposes a new approach using bisphosphonate-conjugated antibiotics to treat bone infections and successfully demonstrates the eradication of Staphylococcus aureus biofilm in a mouse model. The effectiveness of Sitafloxacin BCA in eliminating bacterial biofilm was confirmed, supporting further in vivo drug development.
Article
Multidisciplinary Sciences
Renee Kapteijn, Shraddha Shitut, Dennis Aschmann, Le Zhang, Marit de Beer, Deniz Daviran, Rona Roverts, Anat Akiva, Gilles P. van Wezel, Alexander Kros, Dennis Claessen
Summary: A new endocytosis-like process has been discovered in cell wall-deficient bacteria, allowing them to engulf DNA and other extracellular materials. This process differs from the traditional mechanisms of horizontal gene transfer and requires energy. The findings suggest a possible mechanism for early life forms to acquire essential resources before the development of the bacterial cell wall.
NATURE COMMUNICATIONS
(2022)
Article
Pathology
Rainer Krebs, Jussi M. Tikkanen, Jussi O. Ropponen, Michael Jeltsch, Janne J. Jokinen, Seppo Yla-Herttuala, Antti I. Nykanen, Karl B. Lemstrom
AMERICAN JOURNAL OF PATHOLOGY
(2012)
Article
Cardiac & Cardiovascular Systems
Johanna Valo, Janne J. Jokinen, Markku Kaarne, Leo Ihlberg
ANNALS OF THORACIC SURGERY
(2013)
Article
Cardiac & Cardiovascular Systems
Janne P. Karhunen, Janne J. Jokinen, Peter M. Raivio, Ulla-Stina Salminen
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2011)
Article
Cardiac & Cardiovascular Systems
Janne J. Jokinen, Kalervo Werkkala, Tiina Vainikka, Tarja Perakyla, Jarmo Simpanen, Leo Ihlberg
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2011)
Article
Infectious Diseases
M. Rautio, A. Sipponen, J. Lohi, K. Lounatmaa, P. Koukila-Kahkola, K. Laitinen
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2012)
Article
Dermatology
Arno Sipponen, Opri Kuokkanen, Raine Tiihonen, Harri Kauppinen, Janne J. Jokinen
INTERNATIONAL JOURNAL OF DERMATOLOGY
(2012)
Article
Dermatology
Pentti Sipponen, Arno Sipponen, Jouni Lohi, Marjo Soini, Riikka Tapanainen, Janne J. Jokinen
Article
Surgery
Alireza Raissadati, Janne J. Jokinen, Simo O. Syrjala, Mikko A. I. Keranen, Rainer Krebs, Raimo Tuuminen, Ralica Arnaudova, Eeva Rouvinen, Andrey Anisimov, Jarkko Soronen, Katri Pajusola, Kari Alitalo, Antti I. Nykanen, Karl Lemstrom
TRANSPLANT INTERNATIONAL
(2013)
Article
Cardiac & Cardiovascular Systems
Risto Kesavuori, Antti Vento, Nina Lundbom, Alexey Schramko, Janne J. Jokinen, Peter Raivio
Article
Public, Environmental & Occupational Health
Saija Hyvonen, Jouni Lohi, Tamara Tuuminen
SAFETY AND HEALTH AT WORK
(2020)
Letter
Public, Environmental & Occupational Health
Tamara Tuuminen, Maria Andersson, Saija Hyvonen, Jouni Lohi, Kirsi Vaali
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2020)
Article
Environmental Sciences
Saija Hyvonen, Tuija Poussa, Jouni Lohi, Tamara Tuuminen
Summary: The study found that individuals exposed to indoor air dampness microbiota were at higher risk of developing symptoms related to the central nervous system, fatigue, Multiple Chemical Sensitivity (MCS), cardiac arrhythmia, and respiratory symptoms compared to those unexposed. This suggests that toxic indoor air may lead to a variety of neurological symptoms and broader health issues, emphasizing the importance of addressing indoor air quality.
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH
(2021)
Letter
Public, Environmental & Occupational Health
Saija Hyvonen, Jouni Lohi, Tamara Tuuminen
SAFETY AND HEALTH AT WORK
(2020)
Article
Immunology
Saija M. Hyvonen, Jouni J. Lohi, Leena A. Rasanen, Tuula Heinonen, Marika Mannerstrom, Kirsi Vaali, Tamara Tuuminen
AMERICAN JOURNAL OF CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2020)
