Journal
ULTRASOUND IN MEDICINE AND BIOLOGY
Volume 36, Issue 7, Pages 1098-1108Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2010.04.011
Keywords
Cyclooxygenase-2 (COX-2); Prostaglandin E-2 (PGE(2)); Fracture healing; Low-intensity pulsed ultrasound (LIPUS)
Funding
- Ministry of Science, Education, and Culture of Japan
- Ministry of Health, Labor, and Welfare for Research on the Human Genome, Tissue Engineering, and Food Biotechnology
- Kanagawa Odontological Society
- Grants-in-Aid for Scientific Research [22616009] Funding Source: KAKEN
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To test whether mechanical loading produces faster healing in aged mice, fractured femurs of aged 1-year-old mice were subjected to low-intensity pulsed ultrasound (LIPUS), a treatment that is routinely used to help heal fractures in humans. Cyclooxygenase-2 knockout mice (COX-2(-/-)), which lack an immediate early mediator of mechanical stimulation, were also studied by histochemistry, microcomputed tomography and quantitative polymerase chain reaction to determine the role of COX-2. The healing in the aged COX-2(-/-) mice is slow during the endochondral bone remodeling (>30 d), a period generally prolonged in senescence. For aged wild-type mice, LIPUS halved the endochondral phase to about 10 d, whereas that was not the case for aged COX-2(-/-) mice, which showed no apparent shortening of the prolonged endochondral-phase healing time. Injecting prostaglandin E-2 receptor agonists, however, rescued the COX-2(-/-) callus from insensitivity to LIPUS. In conclusion, COX-2 is a limiting factor in the delayed endochondral bone healing and is induced by LIPUS, which normalizes healing rate to the wild-type level. (E-mail: yukomtak@kdcnet.ac.jp) (C) 2010 World Federation for Ultrasound in Medicine & Biology.
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