Article
Environmental Sciences
Zakaria A. Mohamed, Saad Alamri, Mohamed Hashem, Yasser Mostafa
Summary: This study found that a yeast strain isolated from a lake can eliminate cyanobacteria and their toxin. The yeast removes the toxin by adsorption rather than degradation. The study also showed that heat-inactivated yeast has a higher removal capacity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Sabine Panzer, Chong Zhang, Tilen Konte, Celine Braeuer, Anne Diemar, Parathy Yogendran, Jing Yu-Strzelczyk, Georg Nagel, Shiqiang Gao, Ulrich Terpitz
Summary: Aureobasidium pullulans, a black fungus, possesses genes coding for putative opsins ApOps1, ApOps2, and ApOps3. Heterologous expression and site-directed mutagenesis enabled the creation of light-gated proton channels with potential for optogenetic applications.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Plant Sciences
Izabela Podgorska-Kryszczuk
Summary: Aspergillus flavus is a pathogenic fungus that contaminates many crops with aflatoxins. Researchers have found that two yeast strains, Aureobasidium pullulans PP4 and A. pullulans ZD1, have high potential for biological control of A. flavus. These strains inhibit mycelial growth and spore germination, produce lytic enzymes, and exhibit tolerance to various environmental conditions and fungicides. They also effectively reduce lesion diameter on tomato fruit caused by A. flavus.
Article
Biotechnology & Applied Microbiology
Alessandra Di Francesco, Elena Baraldi
Summary: In this study, Aureobasidium pullulans strains L1 and L8 were shown to compete with Monilinia laxa for iron through the secretion of siderophores, preventing postharvest fruit decay of peaches. These strains demonstrated better efficacy in reducing M. laxa's growth and germination at lower iron concentrations, indicating a potential biocontrol solution for agricultural use.
BIOLOGICAL CONTROL
(2021)
Article
Biotechnology & Applied Microbiology
Luiz Claudio Simoes Correa Junior, Gabrielle Victoria Gauterio, Janaina Fernandes de Medeiros Burkert, Susana Juliano Kalil
Summary: This study successfully established a method for the purification of xylanases and investigated their application in the production of xylooligosaccharides (XOS). Fractional precipitation with ammonium sulfate was found to be more efficient for xylanase purification compared to ethanol precipitation. The purified xylanase showed optimal activity at a temperature of 50°C and a pH of 4.5. Addition of salts such as CaCl2, ZnCl2, and FeCl3 increased the xylanase activity. Xylanase exhibited good thermal stability at 45°C and pH 4.5, and the stability was enhanced in the presence of Ca2+ ions. XOS production from xylan was achieved with a conversion rate of 22.3%. The hydrolysates mainly contained xylobiose, xylotriose, and xylose. The addition of Ca2+ ions did not significantly affect the XOS content, but altered the composition of the hydrolysate.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Daylin Rubio-Ribeaux, Rogger Alessandro Mata da Costa, Renan Murbach Pereira, Paulo Ricardo Franco Marcelino, Fernanda Perpetua Casciatori, Julio Cesar dos Santos, Silvio Silverio da Silva
Summary: This study investigated the impact of LED light on bioemulsifier production by Aureobasidium pullulans LB83 in solid-state fermentation. The results showed that blue light significantly enhanced the production of bioemulsifier and cellulolytic enzyme activity. The bioreactor with blue LED light outperformed others in terms of bioemulsifier production.
FERMENTATION-BASEL
(2023)
Article
Energy & Fuels
Wichanee Bankeeree, Takashi Watanabe, Hunsa Punnapayak, Pongtharin Lotrakul, Sehanat Prasongsuk, Ruibo Li, Dede Heri Yuli Yanto
Summary: The immobilized beta-xylosidase showed high catalytic activity and the synthesized hexyl xyloside exhibited properties comparable to a commercial hexyl glucoside.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Agriculture, Multidisciplinary
Alessandra Di Francesco, Luigi Sciubba, Matteo Bencivenni, Claudio Marzadori, Elena Baraldi
Summary: This study demonstrates the ability of Aureobasidium pullulans strain L1 to increase the bioavailability of Fe (II) in soil by producing siderophores, as well as its promotion of tomato growth and biocontrol efficacy against Rhizoctonia solani. The results suggest potential applications of this microorganism as a biocontrol agent in different environments and against various pathogens.
ANNALS OF APPLIED BIOLOGY
(2022)
Article
Environmental Sciences
Taiele Blumberg Machado, Luiz Claudio Simoes Correa Junior, Mariana Vilar Castro da Veiga de Mattos, Gabrielle Victoria Gauterio, Susana Juliano Kalil
Summary: In this study, different pretreatments of oat hulls were evaluated for xylanase production, with sequential alkaline and ultrasound pretreatments showing the highest enzyme yield. The physical modification of the biomass surface by ultrasound waves, as well as lignin removal and hemicellulose maintenance by alkaline pretreatment, were key factors in the increased xylanase production.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Environmental Sciences
Sashika M. Yalage Don, Joanna M. Gambetta, Christopher C. Steel, Leigh M. Schmidtke
Summary: The study investigated the combined biochemical impact of carbon, nitrogen and temperature on the biosynthesis of antifungal volatile organic compounds produced by Aureobasidium pullulans A1 and A3. Results showed that initial carbon content had a significant influence on the biosynthesis of certain alcohols, while nitrogen availability and growth temperature had negative correlations with VOCs biosynthesis in most cases. The findings provide new insights for enhancing the production of key antifungal compounds by antagonists in biological control systems.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2021)
Article
Instruments & Instrumentation
Vladislav Gamaleev, Naohiro Shimizu, Masaru Hori
Summary: This study introduces an improved high-voltage fast impulse generator that can generate nanosecond-scale high-voltage pulses with high stability and potential for continuous operation. The generator can be used for plasma jet at low temperature, showing promise for biomedical and agricultural applications.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Microbiology
Difan Xiao, Lars M. Blank, Till Tiso
Summary: This article discusses the versatility of the black-yeast-like Aureobasidium as a cell factory for various biotechnological applications. It describes the draft genome sequence of Aureobasidium pullulans NRRL 62031, isolated in Thailand, which spans 25.05-Mb. The genome sequence reveals numerous synthesis pathways for valuable secondary metabolites.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Syahriar Nur Maulana Malik Ibrahim, Wichanee Bankeeree, Sehanat Prasongsuk, Hunsa Punnapayak, Pongtharin Lotrakul
Summary: The crude mannanase from Aureobasidium pullulans NRRL 58524 achieved maximum yield in the optimized medium with 3% defatted spent coffee grounds and 0.67% ammonium sulphate. The enzyme showed thermostability and maximum activity at 55 degrees C and pH 4.0. It also had limited catalytic activity on beta-glucan and was not affected by most ions and chelators. The potential of this enzyme in mannan hydrolysis and mannooligosaccharide production at a larger scale was demonstrated.
Article
Cell Biology
Maria Paula Rueda-Mejia, Lukas Nageli, Stefanie Lutz, Richard D. Hayes, Adithi R. Varadarajan, Igor V. Grigoriev, Christian H. Ahrens, Florian M. Freimoser
Summary: This study demonstrates the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses to identify a limited number of potential target genes for detailed functional analyses.
Article
Chemistry, Applied
Linyu Nian, Mengjun Wang, Min Pan, Shujie Cheng, Wen Zhang, Chongjiang Cao
Summary: This study investigated the antibacterial activity of ultrasound plus high voltage electric field (US&HVEF) on spoilage bacteria and the effect of US&HVEF thawing on the quality characteristics of fish fillets. The results showed that US&HVEF had a strong antibacterial effect against spoilage bacteria and minimized thawing loss, preserved fillet texture, and stabilized the conformation of myofibrillar protein.
Article
Chemistry, Applied
Yue Wu, Wu Li, Enrico Colombo, Gregory J. O. Martin, Muthupandian Ashokkumar
Summary: In this study, the quantitative monitoring of ultrasound-induced inactivation of KTI and BBI was achieved, and its consistency with a traditional assay was verified. The effect of TI concentration, ultrasonic frequency, power density, and pH on the inactivation kinetics of KTI and BBI was explored. The results showed that various factors influenced the inactivation process, including TI concentration, ultrasonic conditions, and pH value.
Article
Chemistry, Applied
Charitha J. Gamlath, Kuan Yen Lo, Thomas S. H. Leong, Muthupandian Ashokkumar, Gregory J. O. Martin
Summary: This study demonstrates the potential of ultrasound emulsification to form whey protein-enriched double emulsions with minimum food-grade emulsifiers to fortify the protein content of cheese and other food products. The encapsulation loading rate of whey proteins within the double emulsion droplets was investigated in relation to ultrasound parameters and formulation loading rates. The results showed that high encapsulation loading rates can be achieved using ultrasound with comparable oil droplet diameters to native milk fat globules.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Physical
Aissa Dehane, Slimane Merouani, Oualid Hamdaoui, Kyuichi Yasui, Muthupandian Ashokkumar
Summary: In sonochemistry, acoustic bubbles serve as microreactors for producing hydrogen and oxidants. This study developed a novel semi-empirical method using hydrogen sono-production to determine the number density of micro-bubbles during water sonolysis. The results showed that the number density of bubbles ranged from -108 to -1013 L-1 s-1, depending on the experimental conditions, and the calculation method used. The study also found that the number of active bubbles increased with frequency, but decreased when calculated via the total single-bubble yield, especially at higher frequencies and colder liquid temperatures.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Acoustics
Yang Shen, Rachel Pflieger, Weizhong Chen, Muthupandian Ashokkumar
Summary: In our previous study, we proposed a new model for single bubble dynamics that considers the effect of bulk viscosity. In this study, we conducted experiments to test this model using a 30% (v/v) glycerol-water mixture. The results showed that the calculated bubble dynamics were not significantly affected by bulk viscosity under the experimental conditions. However, there was a noticeable delay in the first rebound when bulk viscosity was taken into account. The sonoluminescence intensities were also compared between the two models, but no significant difference was observed, confirming the negligible effect of bulk viscosity in this condition.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Food Science & Technology
Shunyu Yao, Wu Li, Yue Wu, Gregory J. O. Martin, Muthupandian Ashokkumar
Summary: Hempseed protein isolate (HPI) can be improved in functionality, such as solubility, emulsifying, and foaming properties, through ultrasonic-assisted extraction. The enhanced functionality is attributed to the changes in protein structure, including increased hydrophobicity and decreased particle size of protein aggregates. Prolonged high-power ultrasound exposure may negatively affect the functional properties of HPI.
Article
Biochemistry & Molecular Biology
Haiyan Zhu, Sukhvir Kaur Bhangu, Muthupandian Ashokkumar, Francesca Cavalieri
Summary: Ultrasound-based engineering has allowed for the synthesis of carrier-free nanodrugs with anticancer and antimicrobial activities using supramolecular self-assembly. High-frequency sound waves were applied to transform small chemotherapeutic and antibiotic drugs into stable nanodrugs without the need for organic solvents or surfactants. Imaging techniques provided insights into the behavior of the nanodrugs inside cells. The findings suggest the potential for small antibiotic drugs to be transformed into chemotherapeutic nanodrugs with high selectivity against cancer cells.
Article
Acoustics
Juhyeon Park, Ahreum Min, Jayaraman Theerthagiri, Muthupandian Ashokkumar, Myong Yong Choi
Summary: Acoustic levitation is a unique tool for levitating and processing droplets and particles. In this study, gold and silver nanoparticles were synthesized using acoustic levitation and pulsed laser irradiation. The synthesized nanoparticles showed excellent catalytic behavior.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Acoustics
Yue Wu, Wu Li, Haiyan Zhu, Gregory J. O. Martin, Muthupandian Ashokkumar
Summary: Liquid-liquid interfacial protein adsorption was proposed as a method to inactivate soy trypsin inhibitors. Ultrasound mixing generated the smallest and most uniform emulsion droplets, resulting in the highest adsorption amount and inactivation percentage. The adsorption amount of trypsin inhibitors increased in the presence of vegetable oil and rapidly increased with sonication time. The pathways of interface-induced inactivation differed for different inhibitors. Overall, ultrasound-assisted interfacial adsorption is a rapid and efficient method to inactivate soy trypsin inhibitors.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Shunyu Yao, Wu Li, Gregory J. O. Martin, Muthupandian Ashokkumar
Summary: Hempseed protein isolate (HPI) has high nutritional value but poor functionality, which has hindered its food application. This study provides important new information on hempseed protein extraction, which may give insights into the extraction of other high-thiol-based plant proteins and improve their functional properties.
APPLIED SCIENCES-BASEL
(2023)
Article
Polymer Science
Amrish Kumar Padmakumar, Vianna F. F. Jafari, Nikhil K. K. Singha, Muthupandian Ashokkumar, Greg G. G. Qiao
Summary: This work describes an alternative method (arm-first approach) to efficiently synthesize core-crosslinked star polymers. The star polymers are produced using a two-step, one-pot Sono-RAFT process. By altering the applied power, the reaction time can be decreased by half. A CCS polymer was produced using a triblock arm, and it exhibited a very high arm-to-star conversion.
JOURNAL OF POLYMER SCIENCE
(2023)
Article
Polymer Science
Amrish Kumar Padmakumar, Nikhil K. Singha, Muthupandian Ashokkumar, Frank A. Leibfarth, Greg G. Qiao
Summary: Advances in nanomolecular engineering have led to the development of technology using ultrasonic irradiation to initiate polymerization. In this study, ultrasound-assisted reversible addition chain-transfer (Sono-RAFT) polymerization was used to compare batch and continuous flow polymerization methods on monomer conversion, polymer molar mass, and dispersity using different monomer substrates. Results showed that continuous flow polymerization had higher monomer conversion compared to batch reactions under the same conditions. Continuous flow reactors with stainless-steel microreactors provided higher cavitation intensity and lower oxygen contamination, making Sono-RAFT possible at higher monomer concentrations. These findings have implications for mechanochemistry and the practical and sustainable implementation of sonochemically regulated polymerization.
Article
Acoustics
Sivakumar Manickam, Daria Camilla Boffito, Erico M. M. Flores, Jean-Marc Leveque, Rachel Pflieger, Bruno G. Pollet, Muthupandian Ashokkumar
Summary: Ultrasonic waves can cause physical and chemical changes in liquid media through acoustic cavitation. The effects of ultrasonics and sonochemistry have been applied in various fields, such as material synthesis, emulsification, cleaning, and processing. The Editors of the Ultrasonics Sonochemistry journal share their perspectives on different aspects of ultrasonics and sonochemistry, providing valuable insights for students and early career researchers.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Polymer Science
Arunjunai R. S. Santha Kumar, Stephanie Allison-Logan, John R. Finnegan, Nikhil K. Singha, Muthupandian Ashokkumar, Greg Qiao
Summary: The effect of ionic liquids on RAFT polymerization was investigated, and a considerable increase in the polymerization rate constants in ionic liquids was observed. Block copolymers with varying block ratios were synthesized with precise control over their molecular weight and chain-end fidelity provided by the photoiniferter polymerization in ionic liquids was described using MALDI-ToF MS analysis.
Review
Food Science & Technology
Vitoria Hagemann Cauduro, Jiwei Cui, Erico Marlon Moraes Flores, Muthupandian Ashokkumar
Summary: Ultrasound-assisted encapsulation is an emerging technique that is used to encapsulate phytochemicals into food products. It has the advantages of producing smaller capsules with lower polydispersity index and higher bioavailability. Further studies should focus on incorporating these capsules into model food products to assess their stability and sensory properties.
Article
Chemistry, Multidisciplinary
Devyani Srivastava, Amita Singh, Gabriele Kociok-Koehn, Suresh W. Gosavi, Ratna Chauhan, Muthupandian Ashokkumar, Abhinav Kumar, Mohd. Muddassir
Summary: Three new heteroleptic Co(iii) ferrocene dithiocarbamate complexes with dppe as ligand have been synthesized and characterized. Single crystal X-ray diffraction revealed a distorted octahedral geometry around Co(iii) in the complexes. The compounds were used as photo-sensitizers in dye-sensitized solar cells and exhibited good photovoltaic performance.
NEW JOURNAL OF CHEMISTRY
(2023)
