Article
Chemistry, Analytical
Yulan Tian, Ping Zhu, Yating Chen, Xiaoyu Bai, Liping Du, Wei Chen, Chunsheng Wu, Ping Wang
Summary: In this study, a novel piezoelectric aptasensor was designed for the detection of OA, with aptamers and quartz crystal microbalance used for signal detection, and gold nanoparticles for signal amplification. The biosensor showed a wide linear detection range and low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biotechnology & Applied Microbiology
Tongtong Chen, Feifei Wu, Yang Li, Hussein E. Rozan, Xiguang Chen, Chao Feng
Summary: A label-free photoluminescence immune-detection platform based on functionalized diatom frustules was developed, showing high sensitivity and a wide detection range. The platform has the potential for sensitive quantitative analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Elena Alina Chiticaru, Celina Maria Damian, Luisa Pilan, Mariana Ionita
Summary: This study proposes a simple, label-free, and cost-effective DNA detection platform using screen-printed carbon electrodes modified with reduced graphene oxide and gold nanoparticles. The preparation process and electrochemical properties of the modified electrodes were characterized and confirmed. The introduction of gold nanoparticles onto the electrode surface enhanced surface conductivity and sensitivity in detection.
Article
Chemistry, Analytical
Shuzhou Guo, Yaping Lin, Ziqi Lian, Tao Zeng, Liangliang Wang, Rongkai Ye, Benlin Hong, Qianwei Liang, Qianqing Wu, Jianqiang Hu, Aiqing Li
Summary: In this study, a label-free ultrasensitive biosensor was successfully developed for instant detection of miRNA-21, which has wide applications in early diagnosis of diseases like cancer and acute kidney injury. The biosensor showed high sensitivity, wide linear range, low limit of detection, and demonstrated specificity, stability, and reproducibility in detecting miRNA-21 in real urine samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Applied
Xiaoxuan Li, Mingdi Jia, Licheng Yu, Yijun Li, Xiwen He, Langxing Chen, Yukui Zhang
Summary: In this study, a novel photonic crystal aptamer biosensor was successfully manufactured, which demonstrated label-free and highly sensitive detection of kanamycin. The biosensor utilized the localized surface plasmon resonance effect of Au NPs and optical response capability of PC to achieve excellent performance in the detection of KANA in complex conditions.
Article
Biophysics
Yangxi Zhang, Hao Wu, Han Wang, Bohan Yin, Siu Hong Dexter Wong, A. Ping Zhang, Hwa-Yaw Tam
Summary: This study presents an ultraminiature biosensor based on nanoparticles and optical fibers, capable of detecting antibodies and testing SARS-CoV-2 mimetic DNA. The biosensor offers a cost-effective solution for various applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Zedong Li, Shuang Wu, Jingcheng Ji, Yuemeng Bai, Pengpeng Jia, Yan Gong, Shangsheng Feng, Fei Li
Summary: Lateral flow assays (LFAs), a popular point-of-care testing platform, have limited detection sensitivity. This study developed a ball pen writing-without-ink method to amplify the detection signal, resulting in improved sensitivity.
Article
Biophysics
Mahdi Malmir, Jalal Arjomandi, Abolfazl Ghafouri Khosroshahi, Mohammadreza Moradi, Hu Shi
Summary: The study introduced a novel biosensor for detecting Y-chromosome DYS14 DNA sequence in the blood plasma of pregnant women, with high efficiency and sensitivity. The results demonstrated the suitability of the biosensor for fetal sex determination in pregnant women between the 7th and 9th weeks of gestation.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Chong Liu, Yuchen Xie, Yingjiao Hou, Ziyi Wang, Xiaoyu Pan, Hong Zhu, Hongna Li
Summary: A selective and sensitive electrochemical enzyme-free sensor for dopamine detection was prepared using carbon nanomaterials, gold nanoparticles, and room-temperature ionic liquid. The study showed that carbon nanotube materials play a key role in catalytic performance for dopamine detection, with single-walled carbon nanotubes exhibiting the highest catalytic effect. pH conditions also influenced the detection of dopamine, uric acid, and ascorbic acid.
Article
Biophysics
Hai-Hua Wang, Meng-Jie Li, Yu-Peng Tu, Hai-Jun Wang, Ya-Qin Chai, Zhao-Hui Li, Ruo Yuan
Summary: This study introduced a novel photoelectrochemical biosensor utilizing a fullerene derivative for amplification-free ultrasensitive detection of single-stranded DNA, demonstrating a wide linear range and low detection limit.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
Clara Perez-Gonzalez, Coral Salvo-Comino, Fernando Martin-Pedrosa, Cristina Garcia-Cabezon, Maria Luz Rodriguez-Mendez
Summary: An innovative bioelectronic tongue (bioET) for milk analysis has been developed, utilizing potentiometric biosensors based on specific enzymes and nanomaterials. By immobilizing enzymes on carboxylated polyvinyl chloride membranes combined with gold nanoparticles, the biosensors exhibit excellent sensitivity and reproducibility. The bioET can efficiently discriminate different classes of milk and simultaneously assess multiple physicochemical parameters, demonstrating its potential in the dairy industry.
Review
Biochemistry & Molecular Biology
Orest Kuntyi, Galyna Zozulya, Andriy Kytsya
Summary: The sonoelectrochemical method is a green synthesis technique for metallic nanoparticles, characterized by manufacturability and environmental friendliness, with the ability to control the geometry of nanoparticles. Ultrasound increases mass transfer and radical formation, accelerating the nucleation and growth of nanoparticles. Combining electrolysis and ultrasound, this hybrid method has attracted significant interest from researchers in the past two decades.
BIOINORGANIC CHEMISTRY AND APPLICATIONS
(2021)
Article
Chemistry, Analytical
Zihao Liu, Wen Zhang, Xin Zhang, Shijia Wang, Zhiwen Xia, Xiaoyan Guo, Yu Zhao, Pu Wang, Xiu-Hong Wang
Summary: This study presents a microstructured optical fiber sensor for rapid, sensitive, and accurate quantification of exosomes in blood samples of breast cancer patients. The sensor utilizes a AuNPs-dsDNA assembly containing gold nanoparticles, a recognizing DNA aptamer, and a fluorescent reporter DNA sequence, which is immobilized on the fiber wall to capture and quantify cancer-derived exosomes. The sensor exhibits enhanced sensitivity and specificity, enabling the detection of single digits of exosome particles at the nanoliter sample level. Additionally, the sensor can track exosome phenotypic changes for precise drug treatment monitoring. This work provides a robust platform for exosome-based biopsy for cancer diagnosis and prediction of therapeutic outcomes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lu Shi, Qiaorong Tang, Bing Yang, Wei Liu, Baoxin Li, Chaoyong Yang, Yan Jin
Summary: A dual-mode DNA logic gate based on gas pressure biosensing and lateral flow assay was developed for the intelligent detection of multiple microRNAs. The gate utilized platinum-coated gold nanoparticles as a signal reporter for dual-signal readout. The miRNAs initiated the cyclic strand displacement reaction to enrich a large amount of nanoparticles, which could be visually detected on a lateral flow strip. Additionally, the nanoparticles catalyzed the decomposition of H2O2 to generate gas pressure, which could be measured by a handheld gas pressure meter.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Fernanda Raquel Wust Schmitz, Karina Cesca, Alexsandra Valerio, Debora de Oliveira, Dachamir Hotza
Summary: Novel rapid methodologies involving colorimetric biosensors and gold nanoparticles have been developed for the detection of bacteria, specifically Pseudomonas aeruginosa. The biosensor relies on the color change of gold nanoparticles induced by NaCl after incubation with bacteria and aptamer binding. The optimized conditions for detection were found to be 5 μM aptamer concentration and 200 mM of NaCl. The detection limit for P. aeruginosa was 10(5) CFU mL(-1) by the naked eye and 10(4) CFU mL(-1) by UV-Vis spectrometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ziqi Xu, Yide Huang, Hao Yin, Xurong Zhu, Ye Tian, Qianhao Min
Summary: This article summarizes DNA origami-based protein manipulation systems, including the classification of DNA nanostructures and the category of assembled proteins. The article reviews the advances in the regulation of these systems' functions and discusses their applications in cellular behavior modulation and disease therapy. The limitations and potential directions of these systems are also presented.
Article
Chemistry, Multidisciplinary
Kai Li, Yanming Cai, Xiaohan Yang, Shuang Wang, Chao Teng, Ye Tian, Qianhao Min, Wenlei Zhu
Summary: In this study, CdS/TiO2:Cu hollow spheres with highly dispersed heterointerfaces were successfully constructed for photoreduction of CO2 in the presence of in situ generated H2S. The production rates of CO and H2 significantly increased due to the consumption of photo-generated holes and large production of protons by H2S decomposition. Moreover, the remediation of photocorroded CdS by H2S extended the service life of the heterojunction.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Analytical
Zehui Jin, Meng Liu, Xiaodan Huang, Xuemeng Zhang, Zexing Qu, Jun-Jie Zhu, Qianhao Min
Summary: This study presents a versatile top-down strategy for rational engineering of carbon material-based matrices to assist in the detection and imaging of small biomolecules using MALDI mass spectrometry imaging. Nitrogen-doped graphene quantum dots were found to have superior capability in assisting the detection of various small molecules, and the proposed negative-ion NGQDs-assisted LDI MSI achieved the imaging of low-MW lipids in rat brain tissue. This work offers a new route for customizing nanomaterial matrices and has important applications in biomedical and pathological research.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Hongmei Xu, Zhenzhen Zhang, Yihan Wang, Xuemeng Zhang, Jun-Jie Zhu, Qianhao Min
Summary: In this study, we proposed fluorophore/mass dual-encoded nanoprobes (FMNPs) that can enable in situ amplified imaging and follow-up mass spectrometric quantification of intracellular multiple miRNAs. The FMNPs were constructed by co-decorating gold nanoparticles with locked hairpin DNA probes and corresponding mass tags for fluorescent and mass spectrometric readout. The results showed that FMNPs can successfully perform miRNA imaging and quantification in different cell lines, highlighting their potential in cell subtyping and accurate clinical diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shan Huang, Yuexin Song, Huiqin Yao, Qianhao Min, Jian-Rong Zhang, Jun-Jie Zhu
Summary: This study presents a nanodevice for sensing and regulating the levels of Zn2+ in dysfunctional islet beta-cells, providing intervention and prognostic assessment for abnormal beta-cell function induced by Zn2+ deposition.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Qianying Zhang, Yihan Wang, Wenjing Wang, Qianhao Min, Jian-Rong Zhang, Jun-Jie Zhu
Summary: Researchers have developed a clever DNA nanomachine that can regenerate in living cells through the activation of telomerase (TE). The nanomachine moves along tracks regenerated by TE and generates amplified signals, allowing for the imaging of apurinic/apyrimidinic endonuclease 1 (APE1) in situ. The regeneration of the nanomachines also reveals differential expression of TE in different cell lines. This study demonstrates the potential of using biomarkers to assist in the regeneration of nanomachines in living cells and opens up new possibilities for the development of more applicable and efficient DNA nanomachines.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Hao Yin, Yanxin Chu, Wei Wang, Zhenzhen Zhang, Zhen Meng, Qianhao Min
Summary: Revealing multiple biomolecules simultaneously in biological and biomedical research is challenging due to their low abundance and poor ionization efficiency. Mass spectrometry (MS) offers unique advantages in multiplexed and label-free analyses, but direct MS profiling of these biomolecules remains difficult. A potential solution to this issue is the use of mass tag (MT)-encoded nanointerfaces, which convert the abundance or activity of biomolecules into amplified ion signals, enabling synchronous MS analysis of multiple targets. This review provides a comprehensive overview of recent advances in MT-encoded nanointerfaces for multiplexed MS bioanalysis and biomedical research, covering their structural characteristics, applications in biomarker assays, cell analysis, and tissue imaging, as well as future prospects.
Article
Chemistry, Multidisciplinary
Huitong Du, Li-Xia Liu, Pan Li, Qianhao Min, Shaojun Guo, Wenlei Zhu
Summary: This study presents an effective strategy for enhancing propanol electro-synthesis from carbon monoxide (CO) reduction through a structural design of Cu2O nanoparticles. The multishell structured catalyst enriches C1 and C2 intermediates by nanoconfinement space, allowing for further coupling and achieving a high Faraday efficiency of 22.22 +/- 0.38% toward propanol at a current density of 50 mA cm-2.
Article
Chemistry, Multidisciplinary
Shan Huang, Yuexin Song, Xiaojun Chen, Qianhao Min, Jian-Rong Zhang, Jun-Jie Zhu
Summary: By incorporating specific recognition, synergistic oxidative damage, and gene therapy, a multimodality therapeutic nanoplatform demonstrates the ability to inhibit the emergence of bacterial resistance and achieve broad-spectrum sterilization activity against multibacterial infections.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Zhenzhen Zhang, Hongmei Xu, Yinyin Fan, Xue Zhang, Wei Wang, Jun-Jie Zhu, Qianhao Min
Summary: In this study, a mass nanotags-enabled interfacial assembly system (MNTs-AS) was developed for multiplexed profiling of RNAs. This system allows simultaneous detection of multiple RNAs in biosystems using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The MNTs-AS-based MS assay shows high sensitivity and multiplexing capability, making it a promising tool for studying biological events and distinguishing cancer subtypes.
Article
Chemistry, Multidisciplinary
Di Gao, Zhuodong Tang, Xueqin Chen, Rong Wu, Ye Tian, Qianhao Min, Jian-Rong Zhang, Zixuan Chen, Jun-Jie Zhu
Summary: We prepared self-assembled DNA nanowires with lengths of 30-120 nm using structural DNA nanotechnology and measured the transport current in these nanowires by plugging individual gold nanoparticles into a circuit. Contrary to previous reports, we observed a significant current attenuation with increasing nanowire length, confirming the prediction of the incoherent hopping model. We also proposed a mechanism for the reversible regulation of charge transport in DNA nanowires involving dynamic transitions in steric conformation.
Article
Multidisciplinary Sciences
Lizhi Dai, Xiaoxue Hu, Min Ji, Ningning Ma, Hang Xing, Jun- Jie Zhu, Qianhao Min, Ye Tian
Summary: Harnessing the programmable nature of DNA origami, researchers have demonstrated the ability to control structural features in crystalline materials and achieve different equilibrium phases and shapes using a single DNA origami morphology. By modulating the binding coordination with an allosteric factor, the DNA origami crystals undergo phase transitions and yield diverse crystal systems. This approach opens up new possibilities for developing crystalline materials with tunable properties and accessing a rich phase space.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Xuemeng Zhang, Weifeng Lu, Cheng Ma, Tao Wang, Jun-Jie Zhu, Richard N. Zare, Qianhao Min
Summary: This article describes a real-time ECL mass spectrometry platform for monitoring dynamic electrical, luminescent, and mass spectrometric outputs during ECL events. It allows the study of ECL pathways and intermediates, and validates two ECL pathways of luminol and a catalytic ECL pathway of boron dipyrromethene (BODIPY).
Article
Chemistry, Multidisciplinary
Huitong Du, Li-Xia Liu, Yanming Cai, Ying Wang, Jian-Rong Zhang, Qianhao Min, Wenlei Zhu
Summary: This study demonstrates the high-performance catalytic ability of N-containing Cu nanoparticles formed in situ by the reconstruction of cuprous 7,7,8,8-tetracyanoquinodimethane in ECOR process, with high FE and current density. The N-containing Cu nanoparticle catalyst showed impressive FE of 81.31% towards multicarbon products with a high partial current density of 162.62 mA cm(-2), presenting promising potential as a Cu-based ECOR catalyst with enhanced artificial carbon fixation ability.
Article
Chemistry, Multidisciplinary
Yuxiang Dong, Zehui Jin, Xue Zhang, Yuanyuan Tang, Ye Tian, Jun-Jie Zhu, Qianhao Min
Summary: A DNA origami cipher disk has been developed to switch between six visually different patterns, forming a cryptographic protocol for accurate information encoding and transmission using DNA strands as carriers.
CHEMICAL COMMUNICATIONS
(2022)
