Journal
ULTRAMICROSCOPY
Volume 145, Issue -, Pages 22-27Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2013.11.012
Keywords
Low-energy electrons; Imaging; Biomolecules; Holography; Reconstruction
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Funding
- Swiss National Science Foundation
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Low-energy electrons (30-250 eV) have been successfully employed for imaging individual biomolecules. The most simple and elegant design of a low-energy electron microscope for imaging biomolecules is a lensless setup that operates in the holographic mode. In this work we address the problem associated with the reconstruction from the recorded holograms. We discuss the twin image problem intrinsic to inline holography and the problem of the so-called biprism-like effect specific to low-energy electrons. We demonstrate how the presence of the biprism-like effect can be efficiently identified and circumvented. The presented sideband filtering reconstruction method eliminates the twin image and allows for reconstruction despite the biprism-like effect, which we demonstrate on both, simulated and experimental examples. (C) 2013 Elsevier B.V. All rights reserved.
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