Article
Optics
Hao Zhu, Zhen Liu, You Zhou, Zhan Ma, Xun Cao
Summary: Lensless imaging is a reliable method for observing microscopic scenes and has various applications. We propose a novel unsupervised Diffractive Neural Field (DNF) method that accurately characterizes the imaging process and achieves precise reconstruction of complex fields using limited measurements. Simulation and experimental results show the superior performance and potential of the proposed method.
Article
Optics
Cheng Xu, Hui Pang, Axiu Cao, Qiling Deng, Song Hu, Huajun Yang
Summary: In this paper, a novel lensless imaging approach based on ptychography and wavefront separation is proposed. This approach has the advantages of rapid convergence and high-quality imaging. By using an amplitude modulator to modulate the light wave, a sequence of modulated intensity images can be acquired for object recovery. Optical experiments were conducted to validate the feasibility of this approach.
Article
Optics
Liming Yang, Ruihai Wang, Qianhao Zhao, Pengming Song, Shaowei Jiang, Tianbo Wang, Xiaopeng Shao, Chengfei Guo, Rishikesh Pandey, Guoan Zheng
Summary: Polarimetric imaging is valuable for inferring birefringence properties of samples without labels. This study presents a novel polarimetric coded ptychography approach that enables high-resolution, high-throughput gigapixel birefringence imaging on a chip. The prototype device can quickly acquire large-scale birefringence images at a low cost.
PHOTONICS RESEARCH
(2023)
Article
Optics
Yue Huang, Shaowei Jiang, Ruihai Wang, Pengming Song, Jian Zhang, Guoan Zheng, Xiangyang Ji, Yongbing Zhang
Summary: This paper proposes the use of accelerated proximal gradient methods for blind ptychographic phase retrieval in lensless on-chip microscopy, dividing the phase retrieval problem into sub-problems involving the evaluation of proximal operator, stochastic gradient descent, and Wirtinger derivatives. The accelerated proximal gradient algorithm shows the best performance in terms of accuracy and convergence rate among the tested algorithms.
Article
Multidisciplinary Sciences
Igor A. Artyukov, Nikolay L. Popov, Alexander V. Vinogradov
Summary: Ptychography is a lensless imaging technology used in the spectral range from hard X-rays to terahertz. In reflection mode ptychography, efficiency can be improved if illumination angles do not exceed the critical angle of the object material. Changing the geometry of the experiment alters the physical and mathematical model of image formation.
Review
Optics
Vivek Boominathan, Jacob T. Robinson, Laura Waller, Ashok Veeraraghavan
Summary: This article reviews recent advances in lensless imaging technology and describes the design principles to be considered when developing and using lensless imaging systems.
Article
Multidisciplinary Sciences
Haozhi Sha, Jizhe Cui, Rong Yu
Summary: In this study, we propose an efficient and robust method to correct specimen misorientation in electron ptychography, enabling deep sub-angstrom resolution imaging for specimens with large misorientations. The method reduces experimental difficulties and paves the way for widespread applications of ptychographic deep sub-angstrom resolution imaging.
Article
Optics
Changchun Lu, You Zhou, Yanxun Guo, Shaowei Jiang, Zibang Zhang, Guoan Zheng, Jingang Zhong
Summary: The study presents a mask-modulated lensless imaging platform based on translated structured illumination for high-resolution complex-valued object image recovery without involving mechanical scanning. By using an LED array to provide angle-varied illumination, regularized ptychographic iterative engine is employed in the reconstruction process, demonstrating higher resolution and better quality recovery compared to previous implementations.
Article
Optics
Zhiming Tian, Ming Zhao, Sen Wang, Ning Zou, Jian Li, Junchao Feng
Summary: This work proposes a novel optimization algorithm and cost function for reconstructing undersampled data in meter-scale reflective Fourier ptychographic imaging. The proposed method achieves the same performance as the alternative-projection-based algorithm but with much less data.
Article
Physics, Applied
Yi Jiang, Junjing Deng, Yudong Yao, Jeffrey A. Klug, Sheikh Mashrafi, Christian Roehrig, Curt Preissner, Fabricio S. Marin, Zhonghou Cai, Barry Lai, Stefan Vogt
Summary: The study demonstrates the use of advanced fly scan ptychography to achieve high-resolution imaging on a large field-of-view at millimeter scale, with significantly reduced imaging time by eliminating stage overheads. The implementation of orthogonal probe relaxation technique helps to overcome illumination variations and local vibrations during the imaging process.
APPLIED PHYSICS LETTERS
(2021)
Article
Optics
Cheng Xu, Axiu Cao, Hui Pang, Qiling Deng, Song Hu, Huajun Yang
Summary: In this paper, a novel multi-height lensless imaging technique is proposed, which replaces the diffuser with a random binary amplitude mask to introduce speckle illumination. Multiple diffraction patterns are recorded by moving the mask axially, and the object and detector remain stationary. The extended ptychographic iterative engine algorithm is adopted to reconstruct the object and unknown mask simultaneously, achieving high-quality reconstruction with as few as 20 images.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Optics
Xiu Wen, Xuyang Zhou, Yutong Li, Yu Ji, Keya Zhou, Shutian Liu, Dong Jia, Wei Liu, Dazhao Chi, Zhengjun Liu
Summary: In this letter, a lensless diffraction imaging method by three-dimensional scanning is proposed, which obtains more diversity of diffraction information through scanning in different directions, achieving high resolution and contrast, large field of view, and simultaneous removal of background noise.
Article
Optics
Qiang Fu, Dong-ming Yan, Wolfgang Heidrich
Summary: This study presents a diffractive lensless camera utilizing spatially-coded Voronoi-Fresnel phase for superior image quality. The design principle focuses on maximizing acquired information in optics to facilitate computational reconstruction, with the optimization guided by an easy-to-optimize Fourier domain metric, MTFv. The proposed Voronoi-Fresnel phase outperforms existing lensless cameras and holds potential for compact imaging systems in extreme physical conditions.
Article
Optics
Xuyang Zhou, Xiu Wen, Yu Ji, Yong Geng, Shutian Liu, Zhengjun Liu
Summary: A fast autopositioning algorithm based on subdivision search is proposed in this paper to calculate the position of each image in coherent diffraction imaging. The method achieves fast and accurate diffraction distance calculation, improving the convergence of iterative phase retrieval and resulting in high-quality reconstructed images.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Biochemical Research Methods
Pengming Song, Chengfei Guo, Shaowei Jiang, Tianbo Wang, Patrick Hu, Derek Hu, Zibang Zhang, Bin Feng, Guoan Zheng
Summary: This study presents a fully on-chip, lensless microscopy technique called optofluidic ptychography, which allows high-resolution quantitative complex imaging in a lab-on-a-chip setup. By integrating ptychographic microscopy into microfluidic devices, the technology can achieve high-resolution ptychographic reconstruction at video framerate and address a wide range of biomedical needs.
Article
Physics, Applied
Ralf Schuster, Othmar Marti
Summary: Mechanical characterization of different cell types is crucial for physiological understanding. Simulations based on the finite element method help understand and improve deformation-based cell characterization methods. The model can track cell deformation in microfluidic channels and simulate various conditions effectively.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Multidisciplinary Sciences
Marios Georgiadis, Aileen Schroeter, Zirui Gao, Manuel Guizar-Sicairos, Marianne Liebi, Christoph Leuze, Jennifer A. McNab, Aleezah Balolia, Jelle Veraart, Benjamin Ades-Aron, Sunglyoung Kim, Timothy Shepherd, Choong H. Lee, Piotr Walczak, Shirish Chodankar, Phillip DiGiacomo, Gergely David, Mark Augath, Valerio Zerbi, Stefan Sommer, Ivan Rajkovic, Thomas Weiss, Oliver Bunk, Lin Yang, Jiangyang Zhang, Dmitry S. Novikov, Michael Zeineh, Els Fieremans, Markus Rudin
Summary: This study demonstrates the use of small-angle X-ray scattering tensor tomography (SAXS-TT) to quantify myelin levels, nanostructural integrity, and axon orientations in nervous tissue. The method was successfully applied to whole mouse brain, mouse spinal cord, and human white and gray matter samples, showcasing its potential in studying myelination in development, aging, disease, and treatment.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Cinzia Giannini, Liberato De Caro, Alberta Terzi, Luca Fusaro, Davide Altamura, Ana Diaz, Rocco Lassandro, Francesca Boccafoschi, Oliver Bunk
Summary: Diseases like diabetes can lead to high sugar concentrations in the body, promoting the formation of glycoconjugates such as glycated collagen. Glucose and galactose produce similar changes in collagen, while ribose is faster in glycation and has qualitatively different effects on collagen structure.
Article
Chemistry, Multidisciplinary
Liberato De Caro, Alberta Terzi, Luca Fusaro, Davide Altamura, Francesca Boccafoschi, Oliver Bunk, Cinzia Giannini
Summary: Glycosylation is a significant process in biochemistry, involving the combination of glucose or other monosaccharides with molecules of different natures. In conditions like diabetes and aging, non-enzymatic glycation of proteins can lead to structural changes in collagen and hinder extracellular matrix degradation.
Article
Instruments & Instrumentation
Juanjuan Huang, Benedikt Guenther, Klaus Achterhold, Martin Dierolf, Franz Pfeiffer
Summary: The paper proposes a setup for simultaneous two-color X-ray absorption spectroscopy at a laboratory-scale synchrotron facility, utilizing inverse Compton scattering technology, with the potential to study reaction mechanisms and synergistic effects of chemical systems containing multiple elements.
JOURNAL OF SYNCHROTRON RADIATION
(2021)
Article
Oncology
Mariele Romano, Alberto Bravin, Alberto Mittone, Alicia Eckhardt, Giacomo E. Barbone, Lucie Sancey, Julien Dinkel, Stefan Bartzsch, Jens Ricke, Marianna Alunni-Fabbroni, Heidrun Hirner-Eppeneder, Dmitry Karpov, Cinzia Giannini, Oliver Bunk, Audrey Bouchet, Viktoria Ruf, Armin Giese, Paola Coan
Summary: This study used a multi-technique approach to analyze the effects of different radiotherapy techniques on healthy and GBM brain tissues, providing a first-time 3D visualization and morphological analysis, as well as quantification and full-organ visualization of tumor volumes and calcium/iron deposits. The multi-scale and multi-technique approach enabled detailed visualization and classification in 3D of the radio-induced effects on brain tissues, bringing new essential information towards the clinical implementation of MRT and MB radiation therapy techniques.
Article
Chemistry, Multidisciplinary
Kirsten Taphorn, Madleen Busse, Johannes Brantl, Benedikt Guenther, Ana Diaz, Mirko Holler, Martin Dierolf, Doris Mayr, Franz Pfeiffer, Julia Herzen
Summary: Quantitative near-field ptychographic X-ray computed tomography is used to assess the location of X-ray stains within cells and identify intracellular structures. The method can be applied to study the distribution of different X-ray stains and compare them to unstained samples. The nanoscopic stain concentrations obtained quantitatively correspond well with dual-energy micro computed tomography measurements.
Article
Computer Science, Interdisciplinary Applications
M. Busse, S. Ferstl, M. A. Kimm, L. Hehn, K. Steiger, S. Allner, M. Mueller, E. Drecoll, T. Buerkner, M. Dierolf, B. Gleich, W. Weichert, F. Pfeiffer
Summary: This article introduces a laboratory-based X-ray method for precise micromorphologic investigation of tissue samples in three dimensions, with the advantages of versatility in multi-scale investigations and nondestructive imaging.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Chemistry, Physical
Alberta Terzi, Nunzia Gallo, Teresa Sibillano, Davide Altamura, Annalia Masi, Rocco Lassandro, Alessandro Sannino, Luca Salvatore, Oliver Bunk, Cinzia Giannini, Liberato De Caro
Summary: Type I collagen scaffold is widely used in tissue engineering and medical applications. The hierarchical structure and multiscale integrity of collagen from different animal sources were studied in relation to tissue-specific regeneration and scaffold design. The structural organization of collagen in tendons was investigated using X-ray scattering techniques and thermal and load-bearing tests.
Letter
Oncology
Carlo Morasso, Renzo Vanna, Francesca Piccotti, Lidia Frizzi, Marta Truffi, Sara Albasini, Camelia Borca, Thomas Huthwelker, Laura Villani, Oliver Bunk, Cinzia Giannini, Fabio Corsi
CANCER COMMUNICATIONS
(2023)
Article
Engineering, Multidisciplinary
Erdal Pekel, Florian Schaff, Martin Dierolf, Franz Pfeiffer, Tobias Lasser
Summary: We present a setup that integrates a seven degrees of freedom robotic arm as a sample holder within an existing laboratory x-ray computed tomography setup. The robotic arm is integrated with a unified software package that allows for path planning, collision detection, geometric calibration and reconstruction of the sample. Experimental results show that the robotic sample holder achieves identical visual quality compared to a conventional sample holder.
ENGINEERING RESEARCH EXPRESS
(2022)
Proceedings Paper
Engineering, Biomedical
Carolin Grandy, Jonas Pfeil, Fabian Port, Kay Gottschalk, Othmar Marti
Summary: Cells are highly sensitive to the mechanical and structural influences of their environment, and micropatterning allows precise regulation of cell forces by altering cell shape. The VELOMIR sensor is an ideal tool for recording the adaptation of cells to pattern shape and changes in viscoelastic properties over an extended period of time. Live cell measurements were taken at a rate of 3000 frames per second for 10 minutes, revealing differences in passive microrheology measurements of mouse embryonic fibroblasts cultured on differently patterned shapes of various sizes.
HIGH-SPEED BIOMEDICAL IMAGING AND SPECTROSCOPY VII
(2022)
Meeting Abstract
Pharmacology & Pharmacy
M. Braune-Yan, L. Kwapich, U. Rupp, H. Barth, O. Marti, C. Read, K. Ernst
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Juanjuan Huang, Fuli Deng, Benedikt Gunther, Klaus Achterhold, Yue Liu, Andreas Jentys, Johannes A. Lercher, Martin Dierolf, Franz Pfeiffer
Summary: X-ray absorption spectroscopy is a crucial technique for catalyst characterization and is typically carried out at synchrotron facilities. This study demonstrates in situ experiments conducted on a palladium-based catalyst at a compact synchrotron facility, showcasing improved catalytic decarboxylation activity.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2021)
Proceedings Paper
Materials Science, Biomaterials
Jonas Pfeil, Tobias Neckernuss, Daniel Geiger, Othmar Marti
Summary: Measuring the mechanical properties of living tissue is challenging due to small sizes and fragility of organisms. Passive microrheology is a promising method for small scales, observing tracer bead motion within the sample. Fast high-speed cameras are needed to resolve frequency-dependent viscoelastic properties, but limitations in data storage lead to short burst measurements. A solution combining a CMOS imaging sensor with an FPGA allows for virtually unlimited long high-speed tracking of multiple particles.
OPTICAL METHODS FOR INSPECTION, CHARACTERIZATION, AND IMAGING OF BIOMATERIALS V
(2021)
Article
Microscopy
C. Gao, C. Hofer, T. J. Pennycook
Summary: Ptychography provides high dose efficiency images that can reveal light elements next to heavy atoms. However, contrast reversals can occur when the projected potential becomes strong. Recent research has shown that these reversals can be counteracted by adapting the focus. This study explains why the best contrast is often found with the probe focused to the middle of the sample and highlights the importance of convergence angle in thin samples to remove contrast reversals.
Article
Microscopy
J. Lindner, U. Ross, T. Meyer, V. Boureau, M. Seibt, Ch. Jooss
Summary: Phase-shifting electron holography is an excellent method with high phase sensitivity to reveal electron wave phase information. An advanced drift correction scheme is proposed in this study, which exploits the interface of the TEM specimen and the vacuum area in the hologram. This method allows for obtaining reliable phase information.
Article
Microscopy
Ali Jaberi, Nicolas Brodusch, Jun Song, Raynald Gauvin
Summary: This study investigates knock-on damage in lithium-ion batteries (LIBs) by computing threshold displacement energies (TDEs) and performing Monte Carlo simulation. The results indicate that Li is most sensitive to knock-on damage at moderate electron energies, and TDE is the principal parameter for assessing Li sensitivity to knock-on damage across similar structures.
Article
Microscopy
Alexander Schroeder, Christopher Rathje, Leon van Velzen, Maurits Kelder, Sascha Schaefer
Summary: This study utilizes novel event-based electron detector platforms to extend the temporal resolution of electron microscopy. By training a neural network to predict electron arrival time, the researchers were able to improve the timing accuracy and achieve a promising solution for enhancing electron timing precision in various electron microscopy applications.
Article
Microscopy
Avi Auslender, Nivedita Pandey, Amit Kohn, Oswaldo Dieguez
Summary: This article describes a faster implementation based on DFT for computing the mean inner potential of crystals, providing quantum-mechanical calculations beyond the independent-atom approximation. The study also reveals the dependence of the mean inner potential on sample boundary conditions, mass density, and magnetic susceptibility, highlighting its correlation with various material properties.
Article
Microscopy
Zhidong Yang, Dawei Zang, Hongjia Li, Zhao Zhang, Fa Zhang, Renmin Han
Summary: In this work, we propose a self-supervised deep learning model for cryo-ET volumetric image denoising based on noise modeling and sparsity guidance. Experimental results demonstrate that our method can achieve reliable denoising by training on single noisy volume and outperform existing methods.
Article
Microscopy
J. Kuttruff, J. Holder, Y. Meng, P. Baum
Summary: In this study, a robust clustering algorithm is proposed that can find clusters in a continuous stream of raw data in real time. This algorithm converts pixel hits measured by hybrid pixel detectors to real single-electron events. By continuously comparing with previous hits, the algorithm efficiently identifies the merging of new and old events.
Article
Microscopy
D. G. Senturk, C. P. Yu, A. De Backer, S. Van Aert
Summary: This article presents a statistics-based method for accurately counting the number of atoms in nanostructures, especially for images acquired with low electron doses and multiple element structures.
Article
Microscopy
Mauricio J. Prieto, Lucas de Souza Caldas, Liviu C. Tanase, Thomas Schmidt, Oscar Rodriguez de la Fuente
Summary: This study presents a synchrotron-based investigation of the synthesis process of a magnetite/hematite bilayer. Ion bombardment gradually transforms hematite into magnetite, and the growth of magnetite leads to the formation of stable boundaries. These findings are significant for understanding novel oxide heterostructures.
Article
Microscopy
Emre Yoruk, Holger Klein, Stephanie Kodjikian
Summary: Beam sensitive nanomaterials pose challenges for crystallographic structure determination. A dose symmetric electron diffraction tomography (DS-EDT) method is developed to reduce beam damage and obtain complete data sets by merging individual datasets from multiple crystals.
Article
Microscopy
A. Pofelski, Y. Zhu, G. A. Botton
Summary: The precision and sensitivity of the GPA method for strain characterization is a widely discussed topic. This study introduces the concept of phase noise and analyzes the parameter of sampling to improve the precision of GPA. Experimental and theoretical results demonstrate that using a larger pixel spacing in STEM can enhance the precision and sensitivity of the GPA method.
Article
Microscopy
Sangjun Kang, Di Wang, Christian Kuebel, Xiaoke Mu
Summary: Transmission electron microscopy is a valuable tool for assessing strain fields within materials. However, using thin specimens in TEM analysis can affect atomic configuration and deformation structure.
