Article
Multidisciplinary Sciences
Bin Li, Jue Liang, Hamid R. Baniasadi, Margaret A. Phillips, Anthony J. Michael
Summary: Viruses manipulate host cells' metabolic and replication systems to produce more viruses. They acquire metabolic genes from ancestral hosts and use the encoded enzymes to subvert host metabolism. This study identified and characterized various phage and virus-encoded polyamine metabolic enzymes and pathways, supporting the important role of spermidine in virus biology on a global scale.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Seongjin Kim, Jeong Ho Chang
Summary: Spermidine is a polyamine molecule that performs various cellular functions and is generated from putrescine by spermidine synthase (SpdS). Although the molecular mechanism of SpdS function has been well-established, its structure-based evolutionary relationships remain to be fully understood. This study determined the crystal structure of an apo-form of SpdS from Kluyveromyces lactis (KlSpdS) and identified a conformational change that provides insights into the structural diversity of SpdS in fungal species.
Article
Marine & Freshwater Biology
Xinxin Lu, Kai Wang, Xiaozhen Mou
Summary: Short-chained aliphatic polyamines are important carbon, nitrogen, and energy sources for marine bacterioplankton. Metagenomic responses of bacterioplankton in the Gulf of Mexico to different PA compounds show that the transformations are carried out by a broad range of taxa and have diverse pathways. PA-transforming genes and taxa vary among different sites, but remain similar among individual compound-amended metagenomes.
MARINE LIFE SCIENCE & TECHNOLOGY
(2022)
Article
Agronomy
Waldemar Kiszczak, Urszula Kowalska, Maria Burian, Malgorzata Podwyszynska, Krystyna Gorecka
Summary: The influence of polyamines (PAs) Putrescine (Put) and Spermidine (Spd) on gynogenesis efficiency in ovule cultures of red beet (Beta vulgaris L. vulgaris) was studied. The response to PAs varied depending on the genotype. Using the PAs in the B5 medium increased the number of obtained embryos in the Czerwona Kula cultivar, with Spd showing stronger effects. Addition of Put to the regeneration medium increased the number of obtained plants. Homozygosity of gynogenetic plants also varied among genotypes. Scoring: 7/10.
Article
Biotechnology & Applied Microbiology
Yongxin Tao, Xiaofei Han, Ang Ren, Jian Li, Hanbing Song, Baogui Xie, Mingwen Zhao
Summary: Under heat stress, spermidine (Spd) plays a stimulatory role in the biosynthesis of ganoderic acids (GAs), with its absolute content being 10 times higher than putrescine (Put). Knockdown mutants experiments confirmed that Spd is a crucial factor leading to the accumulation of GAs under heat stress. This study provides new insights into how environmental stimuli regulate secondary metabolism through polyamines in fungi.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Sheng-Min Liang, Feng-Ling Zheng, Qiang-Sheng Wu
Summary: PAs play a regulatory role in plant physiological activities through beneficial AM fungi, affecting mycorrhizal development and root colonization. Existing research suggests the importance of PAs in the growth of AM fungi and plant root architecture. The presence of AM symbiosis alters endogenous PAs balance, influencing plant physiological activities and stress tolerance.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Piotr T. Bednarek, Renata Orlowska, Dariusz R. Mankowski, Janusz Zimny, Krzysztof Kowalczyk, Michal Nowak, Jacek Zebrowski
Summary: Plant tissue culture techniques are useful for obtaining unique plant materials. This study establishes the relationship between sequence variation, DNA methylation, copper ions, and glutathione in the context of plant regeneration efficiency. The results demonstrate the importance of glutathione in green plant regeneration and suggest that manipulating the concentration of copper ions in the induction medium can improve regeneration efficiency.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Anastasia Mantziari, Enni Mannila, Maria Carmen Collado, Seppo Salminen, Carlos Gomez-Gallego
Summary: The study evaluated the effects of exogenous polyamines on the adhesive properties of various bacterial strains to human intestinal mucus, finding that polyamines may modulate bacterial adhesion to mucus depending on the bacterial strain and the age at which the mucus is generated.
Article
Nutrition & Dietetics
Stefan Senekowitsch, Eliza Wietkamp, Michael Grimm, Franziska Schmelter, Philipp Schick, Anna Kordowski, Christian Sina, Hans Otzen, Werner Weitschies, Martin Smollich
Summary: This study investigated the pharmacokinetics of oral spermidine supplementation for the first time, and found that spermidine supplementation significantly increased plasma spermine levels, while it did not affect spermidine or putrescine levels. This suggests that dietary spermidine is converted into spermine and contributes to the in vitro and clinical effects of spermidine.
Article
Biochemistry & Molecular Biology
Orsolya Kinga Gondor, Judit Tajti, Kamiran Aron Hamow, Imre Majlath, Gabriella Szalai, Tibor Janda, Magda Pal
Summary: This study aimed to investigate the influence of polyamine metabolism under different light conditions. It was found that polyamines had varying effects on photosynthesis under different light regimes, suggesting a complex relationship between polyamines and plant adaptation to light conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Leandro Solmi, Hernan G. Rosli, Marina A. Pombo, Santiago Stalder, Franco R. Rossi, Fernando M. Romero, Oscar A. Ruiz, Andres Garriz
Summary: To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This study investigates how polyamine homeostasis is regulated in the plant environment by examining transcriptomic data of Pseudomonas syringae, an important phytopathogen. The analysis reveals that polyamine synthesis, gene expression activation, and energy production are induced in the early stages of disease, while synthesis is inhibited and transport is upregulated in later stages, correlating with the induction of virulence genes and nitrogen/carboxylic acid metabolism. It is also found that activation of plant defense mechanisms impacts bacterial polyamine synthesis, potentially reducing bacterial fitness in the plant environment. Furthermore, the data suggests that oxidative conditions require a decrease in polyamine production. The implications of these findings are discussed.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Nelly C. Munoz-Esparza, Oriol Comas-Baste, M. Luz Latorre-Moratalla, M. Teresa Veciana-Nogues, M. Carmen Vidal-Carou
Summary: Human milk is rich in polyamines, which are essential for intestinal maturation and immune system development, while infant formulas have significantly lower levels of polyamines compared to human milk. Spermidine and spermine levels in human milk decrease over lactation, while putrescine remains relatively constant.
Article
Biotechnology & Applied Microbiology
Paulami Pramanick, Anindita Chakraborty, Sarmistha Sen Raychaudhuri
Summary: In this study, we investigated the alleviation of zinc-induced stress in Plantago ovata through the exogenous addition of polyamine and its effect on the expression of Metallothionein. Polyamines demonstrated protective and antioxidant properties against zinc-induced stress, reducing lipid peroxidation and down-regulating the expression of Metallothionein. Different concentrations of putrescine and spermidine had varying effects on the total chlorophyll content and antioxidant activities of the plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Genetics & Heredity
Fernando Perez-Rodriguez, Laura Valdes-Santiago, Jorge Noe Garcia-Chavez, Luis Castro-Guillen, Jose Ruiz-Herrera
Summary: Polyamines play important roles in the fungal life cycle and dimorphism. This study investigated the effects of polyamine synthesis mutants on the growth and development of Ustilago maydis. The results showed that high concentrations of putrescine are required for dimorphic transition in yeast cells, while spermidine is essential for mycelium formation. Gene expression analysis identified differentially expressed genes related to pH, genotype, and polyamine concentration. These findings provide valuable insights into the factors involved in polyamine regulation and dimorphism.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Plant Sciences
Rakesh K. Upadhyay, Tahira Fatima, Avtar K. Handa, Autar K. Mattoo
Summary: Polyamines play a positive role in plant growth and development under drought and saline stress conditions. The levels of putrescine, spermidine, and spermine initially increase before decreasing during these stresses, with tomato plants showing survivability for up to 3 and 2 days, respectively. Different forms of polyamines show complex correlations with transcript levels and gene expression, with specific homologs of polyamine biosynthesis and catabolic genes contributing to their accumulation under different stress conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Horticulture
W. Kiszczak, U. Kowalska, M. Burian, K. Gorecka
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2018)
Article
Horticulture
Agnieszka Marasek-Ciolakowska, Justyna Goraj-Koniarska, Urszula Kowalska, Kensuke Miyamoto, Junichi Ueda, Marian Saniewski
SCIENTIA HORTICULTURAE
(2019)
Article
Agriculture, Multidisciplinary
Malgorzata Podwyszynska, Miroslaw Sitarek, Agnieszka Marasek-Ciolakowska, Urszula Kowalska
ZEMDIRBYSTE-AGRICULTURE
(2020)
Article
Biochemistry & Molecular Biology
Agnieszka Marasek-Ciolakowska, Marian Saniewski, Michal Dziurka, Urszula Kowalska, Justyna Goraj-Koniarska, Junichi Ueda, Kensuke Miyamoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Agronomy
Agnieszka Marasek-Ciolakowska, Grazyna Soika, Wojciech Warabieda, Urszula Kowalska, Dariusz Rybczynski
Summary: This study analyzed the morphological and anatomical characteristics of savoy cabbage and kale leaves in relation to their resistance to the cabbage whitefly. Promising sources of resistance to A. proletella were identified among cultivars of savoy cabbage and kale. Further research is needed to confirm the relationship between insect resistance and leaf surface and morphological characteristics in a wider range of Brassica spp.
Article
Biochemistry & Molecular Biology
Agnieszka Marasek-Ciolakowska, Michal Dziurka, Urszula Kowalska, Justyna Goraj-Koniarska, Marian Saniewski, Junichi Ueda, Kensuke Miyamoto
Summary: The study investigated the mode of action of 1-naphthylphthalamic acid (NPA) in inducing conspicuous local stem swelling in intact Bryophyllum calycinum. It was found that NPA mainly stimulated cell division and enlargement in the cambial zone, cortex, and pith. Additionally, comprehensive analyses of plant hormones showed that NPA increased levels of IAA, cytokinins, and jasmonic acid in the swelling area, indicating hormonal crosstalk leading to the observed effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Horticulture
Waldemar Kiszczak, Urszula Kowalska, Maria Burian, Slawa Glinska, Marcin Domaciuk, Krystyna Gorecka
Summary: Yacon micropropagation was optimized in this study. The most effective disinfection method was using 70% ethanol and 0.5% Tween 20. The optimal initial explants were apical and lateral buds cultured on Murashige and Skoog (MS) medium with 0.2 mg L-1 kinetin and 1 mg L-1 indole-3-acetic acid. The most intensive shoot regeneration occurred on MS medium with 1 mg L-1 thidiazuron and 1-naphthylacetic acid. Shoots obtained from in vitro cultures rooted easily on modified MS media with reduced macroelements and supplemented with sucrose and 1-naphthylacetic acid.
ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS
(2022)
Article
Agronomy
Waldemar Kiszczak, Urszula Kowalska, Maria Burian, Malgorzata Podwyszynska, Krystyna Gorecka
Summary: The influence of polyamines (PAs) Putrescine (Put) and Spermidine (Spd) on gynogenesis efficiency in ovule cultures of red beet (Beta vulgaris L. vulgaris) was studied. The response to PAs varied depending on the genotype. Using the PAs in the B5 medium increased the number of obtained embryos in the Czerwona Kula cultivar, with Spd showing stronger effects. Addition of Put to the regeneration medium increased the number of obtained plants. Homozygosity of gynogenetic plants also varied among genotypes. Scoring: 7/10.
Review
Agronomy
Agnieszka Kielkowska, Waldemar Kiszczak
Summary: This paper presents the historical background and current status of haploid induction in carrot, and discusses the potential of haploidization technology in carrot breeding. The methods of androgenesis, gynogenesis, and induced parthenogenesis, as well as the manipulation of the centromere-specific histone 3 variant (CENH3) in carrot, are also acknowledged.
Article
Plant Sciences
Waldemar Kiszczak, Urszula Kowalska, Agata Kapuscinska, Maria Burian, Krystyna Gorecka
ACTA SOCIETATIS BOTANICORUM POLONIAE
(2017)
Article
Agriculture, Dairy & Animal Science
Krystyna Gorecka, Dorota Krzyzanowska, Urszula Kowalska, Waldemar Kiszczak, Malgorzata Podwyszynska
JOURNAL OF CENTRAL EUROPEAN AGRICULTURE
(2017)