Article
Biochemistry & Molecular Biology
Ai Sato, Kiyoshi Takagi, Ayano Yoshimura, Wakana Tsukamoto, Mio Yamaguchi-Tanaka, Yasuhiro Miki, Akiko Ebata, Minoru Miyashita, Takashi Suzuki
Summary: This study explores the clinical significance of KLK12 in breast cancer. The results show that KLK12 is strongly associated with low-grade malignancy and has favorable prognostic effects. Knockdown of KLK12 enhances proliferation and migration of breast cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hayet Bouzid, Feryel Soualmia, Katerina Oikonomopoulou, Antoninus Soosaipillai, Francine Walker, Khaoula Louati, Rea Lo Dico, Marc Pocard, Chahrazade El Amri, Natalia A. Ignatenko, Dalila Darmoul
Summary: KLK6 overexpression in colon cancer is associated with poor prognosis and aggressive behavior. KLK6 activates PAR2, leading to calcium flux and ERK1/2 phosphorylation, promoting tumor formation and invasion. Furthermore, KLK6 abnormal expression can be used to differentiate between malignant and benign ascites.
Article
Multidisciplinary Sciences
Priscilia Lianto, Samantha A. Hutchinson, J. Bernadette Moore, Thomas A. Hughes, James L. Thorne
Summary: A study revealed seven LXR splice variants, five of which were prognostic for disease-free survival in breast cancer patients; while full-length LXR alpha splice variants were associated with poor prognosis, truncated LXR alpha splice variants or any LXR beta splice variant were linked to longer survival.
Article
Biochemistry & Molecular Biology
Layal El-Hajjar, Jessica Saliba, Mario Karam, Abdullah Shaito, Hiba El Hajj, Marwan El-Sabban
Summary: This study investigated the interaction between URM-1 protein and Cx43 and its impact on breast cancer cell progression. It was found that downregulation of URM-1 significantly decreased Cx43 expression and enhanced the migration and invasion potential of breast cancer cells. The results suggest that URMylation of Cx43 regulates its tumor suppression properties and contributes to breast cancer malignancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Jean-Philippe Villemin, Claudio Lorenzi, Marie-Sarah Cabrillac, Andrew Oldfield, William Ritchie, Reini F. Luco
Summary: Breast cancer is a leading cause of death in women globally, with misdiagnosis leading to early metastasis and treatment resistance. While current clinical and gene expression profiles categorize breast tumors, they fail to further subclassify into more subtypes. Analyzing alternative splicing events in breast cancer cell lines can help identify highly aggressive subtypes for further classification.
Article
Oncology
Piotr Ziolkowski, Marta Wozniak, Ahmad Mansour, Yu An, Georg F. Weber
Summary: This study found that in papillomas, fewer than 5% of patients with a pathology score of 0-1 for OPN-c intensity later developed cancer, while almost 18% of patients with a score of 2-3 did. Developing a combined risk score from intensity and positivity for OPN-c showed that patients with low, intermediate, and high scores had progression risks of 3.2%, 5.7%, and 18.8% respectively. Papillomas in patients who later developed cancer displayed stronger staining positivity than non-progressors.
BREAST CANCER RESEARCH
(2022)
Article
Food Science & Technology
Carolina Yukiko Kisaki, Stephanie Santos Suehiro Arcos, Fabio Montoni, Wellington da Silva Santos, Hamida Macedo Calacina, Ismael Feitosa Lima, Daniela Cajado-Carvalho, Emer Suavinho Ferro, Milton Yutaka Nishiyama-Jr, Leo Kei Iwai
Summary: This study investigated quantitative proteomic changes in breast tumor cell lines following treatment with Bothrops jararaca snake venom, showing differential expression of proteins related to cancer cell metabolism, immune response, and inflammation. The data suggest that sub-toxic doses of B. jararaca venom have the potential to modulate cancer-related protein targets in cancer cells, illustrating a novel biochemical strategy to identify therapeutic targets for cancer cell growth and survival.
Review
Cell Biology
Cristina Nuevo-Tapioles, Mark R. Philips
Summary: The three mammalian RAS genes (HRAS, NRAS and KRAS) encode proteins important in cancer biology, with KRAS being the most frequently mutated oncogene. The pre-mRNA of KRAS produces two splice variants, KRAS4A and KRAS4B, which have different membrane targeting sequences. Recent studies have demonstrated the expression of KRAS4A in cancer and identified non-overlapping functions of the splice variants, including direct regulation of hexokinase 1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Hivin Al Kawas, Inas Saaid, Paul Jank, Christina C. Westhoff, Carsten Denkert, Therese Pross, Karoline Barbara Stephanie Weiler, Maria Margarete Karsten
Summary: Altered expression levels and structural variations in VEGF have been found to be important in cancer development and therapy response. Specifically, VEGF splice variants, especially VEGF-A, have been shown to impact physiological and pathological angiogenic processes, with potential implications for targeted antibody-based cancer therapies.
Article
Multidisciplinary Sciences
Marian Kalocsay, Matthew J. Berberich, Robert A. Everley, Maulik K. Nariya, Mirra Chung, Benjamin Gaudio, Chiara Victor, Gary A. Bradshaw, Robyn J. Eisert, Marc Hafner, Peter K. Sorger, Caitlin E. Mills, Kartik Subramanian
Summary: Quantitative proteomics was performed on 60 human-derived breast cancer cell line models, resulting in the identification and characterization of subtypes of breast cancer. The datasets were found to conform to known transcriptional subtypes and are freely available for public use. These datasets can be used in combination with other measurements to predict drug response, provide context in signaling pathway models, and identify markers of sensitivity or resistance to therapeutics.
Article
Genetics & Heredity
Jorge Hermilo Vega Avalos, Luis Enrique Hernandez, Laura Yareni Zuniga, Maria Guadalupe Sanchez-Parada, Ana Elizabeth Gonzalez Santiago, Luis Miguel Roman Pintos, Rolando Castaneda Arellano, Luis Daniel Hernandez-Ortega, Arieh Roldan Mercado-Sesma, Felipe de Jesus Orozco-Luna, Raul C. Baptista-Rosas
Summary: The study found differences in mitochondrial sequences between breast cancer cases and control samples, with certain polymorphic changes significantly more frequent in breast cancer cases compared to controls. These changes are associated with the haplogroup H of Indo-European and Euro-Asiatic origins.
Article
Oncology
Juliana B. Candido, Oscar Maiques, Melanie Boxberg, Verena Kast, Eleonora Peerani, Elena Tomas-Bort, Wilko Weichert, Amiram Sananes, Niv Papo, Viktor Magdolen, Victoria Sanz-Moreno, Daniela Loessner
Summary: Kallikrein-related peptidases are upregulated in pancreatic cancer and associated with poor prognosis. Inhibiting KLK may be a potential therapeutic strategy for precision medicine.
Article
Oncology
Yu An, Gulimirerouzi Fnu, Changchun Xie, Georg F. F. Weber
Summary: This study conducted a comprehensive analysis of splice variants of Osteopontin and found that they have different expression patterns in various cancer patients, which are related to tumor grade, stage, and patient survival. However, there are still some discrepancies that need further investigation to clarify the diagnostic, prognostic, and potentially predictive potential of Osteopontin splice variants.
Article
Biochemistry & Molecular Biology
Woodys Lenga Ma Bonda, Marion Lavergne, Virginie Vasseur, Lucie Brisson, Sebastien Roger, Antoine Legras, Antoine Guillon, Serge Guyetant, Pieter S. Hiemstra, Mustapha Si-Tahar, Sophie Iochmann, Pascale Reverdiau
Summary: KLK5 has been identified as a crucial protease in extracellular matrix remodeling and bronchial epithelial repair after injury. It promotes epithelial-to-mesenchymal transition (EMT) and cell migration by activating the mitogen-activated protein kinase signaling pathway, while also delaying wound healing repair.
Article
Chemistry, Multidisciplinary
Leran Zhang, Scott Lovell, Elena De Vita, Pravin Kumar Ankush Jagtap, Daniel Lucy, Andrea Goya Grocin, Svend Kjaer, Annabel Borg, Janosch Hennig, Aubry K. Miller, Edward W. Tate
Summary: Pancreatic cancer has the lowest survival rate among common cancers due to late diagnosis and limited treatment options. The kallikreinrelated peptidase (KLK) family of secreted serine proteases, specifically KLK6, has emerged as a potential target for pancreatic ductal adenocarcinoma (PDAC). However, the lack of reliable activity-based probes (ABPs) has hindered the validation of these enzymes as targets or biomarkers. In this study, potent and selective ABPs for KLK6 were developed, enabling the detection of KLK6 activity in PDAC cell lines and providing insights into KLK6-mediated invasion pathways.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Panagiotis Tsiakanikas, Andreas Scorilas
Summary: CDK4, an important protein kinase, is implicated in human cancers. Utilizing a novel nanopore sequencing approach, researchers identified previously unknown CDK4 transcripts with potential implications for new diagnostic or therapeutic targets.
Review
Cell Biology
Maria-Alexandra Papadimitriou, Konstantina Panoutsopoulou, Katerina-Marina Pilala, Andreas Scorilas, Margaritis Avgeris
Summary: Methylation of DNA/RNA and proteins is crucial for cellular homeostasis and development, and disrupted methylation imprinting is strongly associated with human pathologies, especially cancer. Recent evidence has shown that miRNAs play a significant role in regulating cellular methylation profiles during cancer onset and progression. Epi-miRNAs are a novel subclass of miRNAs that have potential clinical utility in cancer diagnostics and personalized therapeutics.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Biochemistry & Molecular Biology
Aimilia D. Sklirou, Despoina D. Gianniou, Paraskevi Karousi, Christina Cheimonidi, Georgia Papachristopoulou, Christos K. Kontos, Andreas Scorilas, Ioannis P. Trougakos
Summary: The expression of HSPB2 is significantly downregulated in breast tumors, and high HSPB2 mRNA expression is associated with poor disease-free survival and overall survival in breast cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Maria-Alexandra Papadimitriou, Panagiotis Levis, Georgios Kotronopoulos, Konstantinos Stravodimos, Margaritis Avgeris, Andreas Scorilas
Summary: This study evaluated the clinical utility of preoperative serum cell-free DNA (cfDNA) in risk stratification and prognosis of bladder cancer patients. The results showed that elevated preoperative cfDNA levels were associated with disease progression and metastasis, and were strongly correlated with short-term metastasis and poor prognosis of muscle-invasive bladder cancer (MIBC) patients.
CLINICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Despoina D. Gianniou, Aimilia D. Sklirou, Maria-Alexandra Papadimitriou, Katerina-Marina Pilala, Konstantinos Stravodimos, Margaritis Avgeris, Andreas Scorilas, Ioannis P. Trougakos
Summary: This study investigated the expression and role of HSPB2 and HSPB3 genes in bladder cancer patients and bladder cancer cells. The results showed increased expression levels of HSPB2 and HSPB3 genes and proteins in high-grade bladder cancer cells. In clinical samples, the expression levels of HSPB2 and HSPB3 genes were significantly downregulated in bladder tumors but showed an upregulation trend in muscle-invasive and high-grade tumors. These findings suggest that HSPB2 and HSPB3 have a pro-survival role in advanced bladder cancer and can be targeted as novel molecular markers for treatment optimization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Michaela A. A. Boti, Konstantina Athanasopoulou, Panagiotis G. G. Adamopoulos, Diamantis C. C. Sideris, Andreas Scorilas
Summary: In October 2020, Emmanuelle Charpentier and Jennifer A. Doudna were awarded the chemistry Nobel Prize for their discovery of CRISPR-Cas9, a promising genome-editing tool. The identification of CRISPR arrays and cas genes has led to the development of strategies for precise DNA editing, which have been widely used in biological and medical research.
Review
Biology
Konstantina Athanasopoulou, Glykeria N. Daneva, Michaela A. Boti, Georgios Dimitroulis, Panagiotis G. Adamopoulos, Andreas Scorilas
Summary: Deciphering the etiopathogenesis of cancer is a complex task that requires a multidimensional approach. The introduction of next- and third-generation sequencing technologies has provided powerful tools for cancer research and has expanded the scope of personalized cancer medicine and pharmacogenomics.
Article
Biochemistry & Molecular Biology
Maria Papasavva, Styliana Amvrosiou, Katerina-Marina Pilala, Konstantinos Soureas, Panayiota Christodoulou, Yuan Ji, Konstantinos Stravodimos, Damo Xu, Andreas Scorilas, Margaritis Avgeris, Maria-Ioanna Christodoulou
Summary: Cellular and molecular immune components, including interleukin-37 (IL-37) and its receptor SIGIRR, play a crucial role in the development and progression of bladder urothelial carcinoma (BLCA). IL-37 levels are associated with tumor development and longer overall survival in BLCA patients. Mutations in SIGIRR are related to increased infiltration of regulatory T cells and dendritic cells in the tumor. These findings suggest that IL-37 and SIGIRR could be potential prognostic and diagnostic markers for BLCA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Konstantina Athanasopoulou, Panagiotis G. G. Adamopoulos, Andreas Scorilas
Summary: Over 10(14) symbiotic microorganisms in a healthy human body are responsible for important synthesis and support of the immune system. However, microbial deregulation can lead to various diseases. The human gastrointestinal tract is a favorable environment for diverse microbes. The gut microbiome, the largest microbiome community in the human body, has been extensively studied for its medical significance. Advances in sequencing techniques have enabled the detection of microbiomes and opened new avenues in studying human microbiota. The present review emphasizes the complexity of the gastrointestinal tract microbiome and its implications in cellular homeostasis and human diseases.
Article
Genetics & Heredity
Paraskevi Karousi, Christos K. Kontos, Panagiota Papakotsi, Ioannis K. Kostakis, Alexios-Leandros Skaltsounis, Andreas Scorilas
Summary: This study found that oleuropein and oleocanthal can inhibit the proliferation of triple-negative breast cancer (TNBC) cells and significantly alter the expression profile of TNBC cells. These compounds may have therapeutic benefits for TNBC through their effects on multiple pathways associated with TNBC.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Paraskevi Karousi, Pinelopi I. Artemaki, Ahmed Abdelgawad, Aspasia Dimitriadou, Nikolaos P. Machairas, Diamantis C. Sideris, Vasiliki Pappa, Andreas Scorilas, Mona Batish, Sotirios G. Papageorgiou
Summary: In this study, we identified several novel circRNAs produced by BAX and BCL2L12, characterized by diverse exon structures. Interestingly, visualization of the most abundant circRNAs showed distinct intracellular localization. Additionally, a complex expression pattern of BAX and BCL2L12 circRNAs was revealed in CLL patients and non-leukemic blood donors, suggesting a multifaceted role in B-cell CLL.
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Despina Hadjichambi, Maria Papatsirou, Paraskevi Karousi, Spyridon Christodoulou, Diamantis C. Sideris, Andreas Scorilas
Summary: This study identifies 23 novel BOK circRNAs, which consist of both exonic and intronic regions of the BOK gene. These circRNAs could play a role in the molecular landscape and clinical investigation of ovarian and prostate cancer.
Review
Biochemistry & Molecular Biology
Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Glykeria N. Daneva, Andreas Scorilas
Summary: Recent advances in molecular biology have allowed for the identification of post-transcriptional marks on RNAs, leading to the emerging field of epitranscriptomics. Over 170 RNA modifications have been reported, playing crucial roles in RNA biogenesis, stability, and accuracy of transcription and translation. Dysfunctions in the enzymes responsible for RNA modifications can lead to human diseases and cancer. This review focuses on the landscape of epitranscriptomics in human RNAs, describing the most common modifications and their functional and regulatory roles, as well as their involvement in cellular homeostasis and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Katerina Katsaraki, Christos K. Kontos, Gerasimos Ardavanis-Loukeris, Alexandros A. Tzovaras, Diamantis C. Sideris, Andreas Scorilas
Summary: This study investigates the regulatory effect of proteasome inhibitors (PIs) on miRNA expression in breast cancer (BrCa) cell lines. The results show a high heterogeneity in miRNA levels among different cell lines and different treatment durations. Three miRNAs (miR-1-3p, miR-421-3p, and miR-765-3p) are identified as potential key players in BrCa. Various biological processes, molecular functions, and pathways are predicted to be affected.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Genetics & Heredity
Paraskevi Karousi, Martina Samiotaki, Manousos Makridakis, Jerome Zoidakis, Diamantis C. Sideris, Andreas Scorilas, Thomas Carell, Christos K. Kontos
Summary: This study investigates the role of 3'-tRF-Cys(GCA) in gene expression regulation in HEK-293 cells and finds that it can regulate gene expression by affecting multiple cellular pathways. Additionally, the study identifies the direct interaction between 3'-tRF-Cys(GCA) and thymopoietin and its modulation of the protein's levels.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
