4.2 Article

Sub-speciation of Mycobacterium tuberculosis complex from tuberculosis patients in Japan

Journal

TUBERCULOSIS
Volume 94, Issue 1, Pages 15-19

Publisher

CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tube.2013.09.006

Keywords

Mycobacterium tuberculosis complex; Mycobacterium africanum; Mycobacterium bovis; Multiplex-PCR; MTC differentiation

Funding

  1. Health Science Research Grant of Ministry of Health, Welfare and Labour [H20-Sinko-Saiko]

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Mycobacterium tuberculosis is the major causative agent of tuberculosis in humans. It is well known that Mycobacterium bovis and other species in the M. tuberculosis complex (MTC) can cause respiratory diseases as zoonosis. We analyzed the MTC isolates collected from tuberculosis patients from Japan in 2002 using a multiplex PCR system that detected cfp32, RD9 and RD12. A total of 970 MTC isolates that were representative of the tuberculosis cases throughout Japan, were examined using this method. As a result, 966 (99.6%) M. tuberculosis, two Mycobacterium africanum and two Mycobacterium canettii were identified using a multiplex PCR system, while no M. bovis was detected. Two isolates that lacked RD9 were initially considered to be M. canettii, but further analysis of the hsp65 sequence revealed them to be M. tuberculosis. Also two M. africanum were identified as M. tuberculosis using the -215 narG nucleotide polymorphism. Though PCR-linked methods have been used for a rapid differentiation of MTC and NTM, from our cases we suggest careful interpretation of RD based identification. (C) 2013 Elsevier Ltd. All rights reserved.

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