Journal
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Volume 13, Issue 2, Pages 225-228Publisher
PHARMACOTHERAPY GROUP
DOI: 10.4314/tjpr.v13i2.9
Keywords
Cirsium japonicum; Anti-proliferation activity; Pectolinarigenin; Breast cancer; Apoptosis
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Purpose: To isolate high purity pectolinarigenin from Cirsium japonicum and evaluates its anti-proliferative potential and induction of apoptosis activity in MCF-7 breast cancer cell. Methods: Pectolinarigenin was purified by the combination of silica gel and Sephadex LH-20 column chromatography. The structure was identified by 1 H-nuclear magnetic resonance (NMR), C-13-NMR and electrospray ionization tandem mass spectrometry (ESI-MS) analyses. The anti-proliferation activity of pectolinarigenin was also evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and colony formation assays in MCF-7 breast cancer cell. Results: Pectolinarigenin showed potent anti-proliferation activity by inducing apoptosis and downregulation of Bcl2 expression in MCF-7 breast cancer cell. Protein expression of Bcl2 was almost completely inhibited upon the treatment with 25 m M pectolinarigenin. Conclusion: A rapid method to isolate high purity pectolinarigenin from C. japonicum and as well as evaluate its anti-proliferative potential and induction of apoptosis in MCF-7 breast cancer cells is presented for the first time. Induction of apoptosis of MCF-7 is via downregulation of Bcl2 expression.
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