Journal
TROPICAL ANIMAL HEALTH AND PRODUCTION
Volume 42, Issue 8, Pages 1597-1599Publisher
SPRINGER
DOI: 10.1007/s11250-010-9616-0
Keywords
Trypanosomiasis; Bovine; Polymerase chain reaction; T. evansi
Funding
- Government of Punjab
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The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENTA (R). The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.
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