Article
Horticulture
Lauren E. Kurtz, Lillian N. Borbas, Mark H. Brand, Jessica D. Lubell-Brand
Summary: This study evaluated the success of ex vitro rooting for micropropagated cannabis plants and found that rooting success declined with increasing culture age. Micropropagated 'Wife' plants exhibited similar growth characteristics to stem cuttings, while micropropagated 'Abacus' plants performed equally as well when given an extra week of vegetative growth. Retip cuttings showed potential as a propagation method, with high rooting rates comparable to stem cuttings treated with rooting hormone. Retip cuttings also possessed desirable characteristics similar to micropropagated plants.
Article
Agronomy
Yaser Hassan Dewir, Ali Mohsen Al-Ali, Hail Z. Rihan, Thobayet Alshahrani, Mona S. Alwahibi, Khalid F. Almutairi, Yougasphree Naidoo, Michael P. Fuller
Summary: Different LED light conditions were tested for in vitro culture of axillary blackberry shoots, and the combination of blue and red light (2:1 spectral ratio) with 30 g L-1 of sucrose was found to be the most effective for rooting and vegetative growth. Sucrose supplementation was essential for root development, but high concentrations negatively affected leaf area and growth. LED treatments resulted in increased leaf pigments compared to fluorescent treatment.
Article
Horticulture
Konstantinos Bertsouklis, Konstantina-Panagiota Panagaki
Summary: This study aimed to establish a micropropagation protocol for the endangered plant Dyckia brevifolia. By investigating the germination of seeds and the effects of different cytokinin concentrations on seedlings, the researchers successfully achieved large-scale propagation of this species.
Review
Horticulture
Laxmi Chilukamarri, Seyedardalan Ashrafzadeh, David W. M. Leung
Summary: In-vitro grafting involves grafting a micro-scion onto a micro-rootstock under aseptic conditions, offering advantages over traditional grafting methods. It has been primarily used for producing virus-free plants and studying scion-rootstock interactions, long-distance signaling, and virus-indexing.
SCIENTIA HORTICULTURAE
(2021)
Article
Biotechnology & Applied Microbiology
Massimo Zacchini, Giorgio Gullotta, Gianluca D'Onofrio, Pierangelo Bertolotto, Lorenzo Massimi, Fabrizio Pietrini
Summary: This study demonstrated the potential of hemp plants to tolerate and accumulate lithium, although lithium showed toxic effects on plant growth and impacted photosynthetic machinery and element uptake.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Vera Pavese, Paola Ruffa, Silvia Abba, Rita Lourenco Costa, Elena Corredoira, Cristian Silvestri, Daniela Torello Marinoni, Roberto Botta
Summary: Castanea sativa cv. 'Garrone Rosso' and 'Marrone di Castel del Rio' are two prized varieties in Italy. This study presents an in vitro micropropagation protocol for these varieties to preserve and enhance the Italian germplasm, which is important due to constraints in plant propagation for orchard renewal and new planting.
Article
Multidisciplinary Sciences
Saleh Amiri, Reza Mohammadi
Summary: Efficient micropropagation protocol for Rhus coriaria using lateral buds as explants was established by optimizing plant growth regulators in MS medium, resulting in high rates of establishment, shoot proliferation, and rooting, which can be utilized for mass propagation.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Kathleen Terhaag, Juliana Klostermann Ziemmer, Clarissa Alves Stefanello, Felipe Astolpho de Almeida, Vanildo Silveira, Leila do Nascimento Vieira, Hugo Pacheco de Freitas Fraga
Summary: Araucaria angustifolia is an economically important conifer that plays a fundamental role in ecological and ethnoecological relationships. The forest of this species is highly fragmented and at a critical risk of extinction due to excessive exploitation. This study aimed to establish callus cultures of A. angustifolia using different parts of the plant as explants and investigate the embryogenic potential of these cultures through morphological and proteomic analyses. The results showed that callus cultures could be generated from both orthotropic and plagiotropic apexes, with different morphological characteristics and proteomic profiles.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Multidisciplinary Sciences
Oezhan Simsek, Dicle Donmez, Mehmet Ali Sarida, Emine Acar, Yildiz Aka Kacar, Sevgi Paydas Kargi, Tolga Izgu
Summary: This study evaluated and compared different propagation systems for myrtle genotypes and found that the Plantform system was highly successful in micropropagation and rooting. This contributes to year-round propagation and conservation of genetic resources.
Article
Forestry
Leandro Silva de Oliveira, Gilvano Ebling Brondani, Leticia Vaz Molinari, Rafaella Zanetti Dias, Gustavo Leal Teixeira, Antonio Natal Goncalves, Marcilio de Almeida
Summary: This study evaluated the effect of NAA, TDZ, and BAP on the in vitro regeneration of Eucalyptus cloeziana. The results showed that cotyledonary leaves were the most suitable tissue for regeneration, and TDZ-induced callus facilitated the formation of adventitious buds. In the differentiation stage, a concentration of 4.4 μM BAP promoted the regeneration of adventitious buds. This study establishes an efficient protocol for the in vitro regeneration of E. cloeziana.
JOURNAL OF FORESTRY RESEARCH
(2022)
Article
Plant Sciences
Luca Regni, Maurizio Micheli, Alberto Marco Del Pino, Carlo Alberto Palmerini, Roberto D'Amato, Simona Lucia Facchin, Franco Famiani, Alessandro Peruzzi, Hanene Mairech, Primo Proietti
Summary: The study investigated the effects of different selenium concentrations on olive explants and found that selenium can promote plant growth within a certain concentration range. Different olive varieties responded differently to selenium, and the effectiveness of selenium decreased with the number of subcultures.
Article
Agronomy
Duong Van Yen, Jing Li
Summary: This research article presents the results of in vitro growth experiments on Stahlianthus thorelii Gagnep, a plant belonging to the family Zingiberaceae, using tubers as explants and specific growth stimulant concentrations in MS media. The study successfully increased the number of shoots and the root/shoot ratio through a series of cultivation techniques, achieving 100% survival rate when acclimatized in soil conditions.
Article
Horticulture
Behzad Kaviani, Bahareh Deltalab, Dariusz Kulus, Alicja Tymoszuk, Hamideh Bagheri, Taha Azarinejad
Summary: This study presents an efficient in vitro propagation method for narrow-leaf firethorn, using different hormones and culture media for axillary shoot multiplication and root induction.
Article
Biology
Sadaf Rafiq, Z. A. Rather, Reyaz Ahmad Bhat, I. T. Nazki, Mohammad S. AL-Harbi, Neelofar Banday, Iqra Farooq, Bassem N. Samra, M. H. Khan, Atef F. Ahmed, Najmah Andrabi
Summary: In this study, a micropropagation protocol for Oriental Hybrid Lilium cv. Ravenna was developed using bulb scale segments as explants. Through a series of sterilization processes and the use of appropriate growth regulators, the culture establishment rate and explant survival rate were significantly improved, leading to efficient shoot proliferation and rooting effects.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Plant Sciences
Vikas Sharma, Shivika Ankita, Arun Karnwal, Shivika Sharma, Barkha Kamal, Vikash S. Jadon, Sanjay Gupta, Iyyakkannu Sivanasen
Summary: The genus Eucalyptus is a globally sought-after hardwood source known for its medicinal uses. Its hybrid and wild species are widely used in exotic plantations due to their adaptability and rapid propagation. Although biotechnological and tissue culture approaches have progressed, low efficiency in clonal propagation still limits plantation productivity.
Article
Agronomy
H. Nobrega, G. Freitas, M. A. Zavattieri, C. Ragonezi, L. Frese, M. A. A. Pinheiro de Carvalho
Summary: The research conducted on Beta patula species in Madeira Archipelago included a complete floristic survey on two islands to understand the population dynamics and ecological requirements, as well as calculating biodiversity indices to assess the vegetation. The demographic status of Beta patula populations on the islands was determined yearly between 2014 and 2018, providing data for the establishment of a protocol to monitor and manage a genetic reserve for Beta patula and other crop wild relatives. This work aims to contribute to the implementation of the European genetic reserve network.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Review
Green & Sustainable Science & Technology
Nuno Nunes, Carla Ragonezi, Carla S. S. Gouveia, Miguel A. A. Pinheiro de Carvalho
Summary: Sewage sludge, an underused biomass derived from wastewater treatment plants, can be utilized as biofertilizer after proper stabilization due to its high content of inorganic matter, nitrate, and phosphorus. However, a wide range of pollutants may be present in sewage sludge, which could have detrimental effects on the environment with long-term applications. Proper screening for heavy metals is essential when utilizing sewage sludge, along with adherence to guidelines and regulations for its application.
Article
Biodiversity Conservation
Humberto Nobrega, Gregorio Freitas, M. A. Zavattieri, Carla Ragonezi, Miguel A. A. Pinheiro de Carvalho
Summary: This study summarizes the in situ and ex situ conservation research on the critically endangered species Beta patula, focusing on the associated plant communities. Valuable data was obtained through vegetation census on uninhabited islets Desembarcadouro and Chao, which will contribute to the implementation of conservation actions and management protocols. This work is part of the EU LIFE Project LIFE RECOVER NATURA and was conducted by members of the ISOPlexis Center at the University of Madeira.
BIODIVERSITY DATA JOURNAL
(2021)
Article
Plant Sciences
Carlos G. Andrade, Emanuel M. da Silva, Carla Ragonezi, Miguel A. A. Pinheiro De Carvalho
Summary: The study aimed to screen Begomovirus, Potyvirus, and Carlavirus infections in 16 traditional sweet potato cultivars. The infection prevalence among cultivars was high, with some showing vulnerability to multiple viral infections. A new divergent strain of the SPCFV species was identified in one of the accessions.
NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
(2021)
Article
Agronomy
Lidiomar Soares da Costa, Paulo Henrique Grazziotti, Arley Jose Fonseca, Debora Cintia dos Santos Avelar, Marcio Jose Rossi, Enilson de Barros Silva, Eliane Cristine Soares da Costa, Danielle Cristina Fonseca Santos Grazziotti, Carla Ragonezi
Summary: Ectomycorrhizae are classified as a biotechnology to increase the sustainability of planted forests, with fieldwork needed to confirm effectiveness. Inoculation of Eucalyptus clones with ectomycorrhizal fungi in the nursery boosts early plant growth after planting in the field, depending on the specific fungus and clone. Ectomycorrhizal colonization of Eucalyptus occurs naturally and increases with tree development in the field.
Article
Agronomy
Carla Ragonezi, Nuno Nunes, Maria Cristina O. Oliveira, Jose G. R. de Freitas, Jose Filipe T. Gananca, Miguel A. A. Pinheiro de Carvalho
Summary: This study evaluated the application of sewage sludge on sweet potato production and soil improvement. The results showed that sewage sludge application effectively improved the agronomic parameters of sweet potato and enhanced soil features.
Article
Agronomy
Miguel A. A. Pinheiro de Carvalho, Carla Ragonezi, Maria Cristina O. Oliveira, Fabio Reis, Fabricio Lopes Macedo, Jose G. R. de Freitas, Humberto Nobrega, Jose Filipe T. Gananca
Summary: The Climate-Madeira Strategy (CMS) predicts climate scenarios for the Madeira region in 2050 and 2070, including an increase in average temperature and a decrease in precipitation. This study establishes a baseline for a typical agricultural system in Madeira and investigates the impacts of climate change on its components and productivity.
Article
Agronomy
Carla Ragonezi, Humberto Nobrega, Maria Ines Leite, Jose G. R. de Freitas, Fabricio Lopes Macedo, Miguel A. A. Pinheiro de Carvalho
Summary: This study presents the spatial distribution of genetic variability and diversity in B. patula populations using eight polymorphic SSR markers. Despite some genetic differences, the CI and DI populations have more similarities than differences. Analysis of the Molecular Variance showed that 9% of the diversity is between populations, 68% among individuals, and 23% within individuals.
Article
Agronomy
Fabricio Lopes Macedo, Humberto Nobrega, Jose G. R. de Freitas, Carla Ragonezi, Lino Pinto, Joana Rosa, Miguel A. A. Pinheiro de Carvalho
Summary: The advancement of technology in the field, especially the use of UAVs and multispectral cameras, allows real-time monitoring of crop conditions and contributes to machine learning. This study aimed to estimate corn crop productivity and above-ground biomass using different vegetation indices from high-resolution aerial imagery. Strong correlations were found between productivity and the NDVI (p < 0.05; 0.719), as well as the NDRE and GNDVI with crop productivity (p < 0.01; 0.809 and 0.859). The results for above-ground biomass were consistent with previous findings, with high correlations for GNDVI and NDRE (p < 0.05; 0.758 and 0.695). The GNDVI and NDRE indices showed good coefficients of determination for productivity and above-ground biomass estimation. Predicting crop productivity is crucial for producers, allowing them to make timely decisions and contribute to AI integration for drone optimization.
Article
Agronomy
Fabricio Lopes Macedo, Carla Ragonezi, Fabio Reis, Jose G. R. de Freitas, David Horta Lopes, Antonio Miguel Franquinho Aguiar, Delia Cravo, Miguel A. A. Pinheiro de Carvalho
Summary: This study investigated the potential distribution of Drosophila suzukii on Madeira Island using Maximum Entropy modeling and found that altitude was the dominant factor affecting the distribution, with elevations ranging from 0 to 500 m being the most suitable for the species.
Article
Agriculture, Multidisciplinary
F. Ascarini, H. G. M. Nobrega, I. S. Leite, G. Freitas, C. Ragonezi, M. Amely Zavattieri, M. A. A. Pinheiro de Carvalho
Summary: Sea beet, a morphologically and genetically variable species, is a valuable genetic resource for resistance improvement in beets. Characterization of Madeiran sea beet populations revealed high quantitative variation among populations, with plant height and inflorescence height parameters having the greatest influence on population separation. Molecular analysis using SSRs showed genetic variability between populations, with PCA and PCoA identifying three clusters based on morphological and genetic traits, highlighting the influence of specific environmental conditions on genetic variability and the potential for these populations to be gene sources for sugar beet breeding programs.
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Mario Rui da Costa Basilio e Castro, Carla Ragonezi, Paulo Guilherme Leandro de Oliveira, Maria Amely Zavattieri
METHODS AND PROTOCOLS
(2019)