Journal
TOXINS
Volume 7, Issue 6, Pages 2024-2050Publisher
MDPI
DOI: 10.3390/toxins7062024
Keywords
ergot; LSD; ergotamine; lysergic acid; ergovaline; mycotoxins
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The principles and application of established and newer methods for the quantitative and semi-quantitative determination of ergot alkaloids in food, feed, plant materials and animal tissues are reviewed. The techniques of sampling, extraction, clean-up, detection, quantification and validation are described. The major procedures for ergot alkaloid analysis comprise liquid chromatography with tandem mass spectrometry (LC-MS/MS) and liquid chromatography with fluorescence detection (LC-FLD). Other methods based on immunoassays are under development and variations of these and minor techniques are available for specific purposes.
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