Article
Multidisciplinary Sciences
Nicolas Pavillon, Nicholas I. Smith
Summary: The monitoring of dynamic cellular behaviors is difficult due to the limitations of current techniques for single-cell analysis. In this study, we use label-free optical techniques to non-invasively monitor the changes in murine naive T cells during activation and differentiation. By analyzing spontaneous Raman single-cell spectra, we develop statistical models to detect activation and use non-linear projection methods to track changes during early differentiation. Our label-free results show high correlation with known surface markers and provide spectral models to identify the underlying molecular species involved in the biological process under study.
SCIENTIFIC REPORTS
(2023)
Review
Biochemical Research Methods
Kai Pang, Sihan Dong, Yuxi Zhu, Xi Zhu, Quanyu Zhou, Bobo Gu, Wei Jin, Rui Zhang, Yuting Fu, Bingchen Yu, Da Sun, Duanmu Zheng, Xunbin Wei
Summary: Flow cytometry is a versatile tool for detecting and measuring characteristics of cells or particles. Recent advancements in techniques such as in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC have expanded the applications of FC in basic research and clinical practice.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Cell Biology
Lorenz Adlung, Paul Stapor, Christian Toensing, Leonard Schmiester, Luisa E. Schwarzmueller, Lena Postawa, Dantong Wang, Jens Timmer, Ursula Klingmueller, Jan Hasenauer, Marcel Schilling
Summary: Survival or apoptosis is a binary decision at the individual cell level, but at the cell-population level, a graded increase in survival can be observed. The variability in membrane-associated processes, such as Epo receptor complexes and cell volume, can convert switch-like behavior at the single-cell level to a graded population-level response. Variability in nuclear phosphorylated STAT5 levels plays a key role in individual cell survival.
Article
Biochemical Research Methods
Jessie E. Arce, Joshua A. Welsh, Sean Cook, John Tigges, Ionita Ghiran, Jennifer C. Jones, Andrew Jackson, Matthew Roth, Aleksandar Milosavljevic
Summary: The NanoFlow Repository, the first implementation of the MIFlowCyt-EV framework, has been developed to meet the need for data sharing and minimum reporting standards for extracellular particles (EPs).
Article
Biochemical Research Methods
Paul H. Dannenberg, Jisoo Kang, Nicola Martino, Anokhi Kashiparekh, Sarah Forward, Jiamin Wu, Andreas C. Liapis, Jie Wang, Seok-Hyun Yun
Summary: This article demonstrates a cell barcode technology using laser particles, which can be used for cell sorting and routing through a microfluidic platform integrated with a high-speed spectrometer. By utilizing the spectral emission of laser particles, individual cells labeled with different laser colors can be distinguished and sorted.
Article
Cardiac & Cardiovascular Systems
David J. Schneider, Heidi S. Taatjes-Sommer, Jayne Prats, Efthymios N. Deliargyris
Summary: The study showed that the pharmaceutical lipid excipients used in a novel oral formulation with aspirin do not impair the pharmacodynamic effects of aspirin or alter platelet function. Additionally, when the inhibition of platelet aggregation by aspirin was limited, the excipients tended to enhance its pharmacodynamic effects.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2021)
Review
Pharmacology & Pharmacy
Chelsea B. Pratt, Matt Alexander
Summary: The development of CAR-T cells is crucial for the success of adoptive T-cell immunotherapy. While the process of producing autologous CAR-T cells is time-consuming and expensive, the exploration of cost-effective allogeneic CAR-T cells is essential. High-throughput tools are used to monitor the entire process of CAR-T cell design, testing, manufacturing, and patient monitoring to ensure safety and efficacy.
DRUG DISCOVERY TODAY
(2022)
Article
Immunology
Ulrich Blache, Ronald Weiss, Andreas Boldt, Michael Kapinsky, Andre-Rene Blaudszun, Andrea Quaiser, Annabelle Pohl, Tewfik Miloud, Megane Burgaud, Vladan Vucinic, Uwe Platzbecker, Ulrich Sack, Stephan Fricke, Ulrike Koehl
Summary: The adoptive immunotherapy using CAR-T cells has shown successful remissions in refractory B-cell leukemia and B-cell lymphomas, but it is important to monitor both success and potential severe side effects of this therapy. Research has been conducted to establish standardized flow cytometry assays to characterize immune cell subpopulations in the peripheral blood during CAR-T cell treatment, with the possibility of adapting these assays for quality controls in the CAR-T cell manufacturing process in the future.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Jana H. Badrani, Allyssa N. Strohm, Yung -An Haung, Taylor A. Doherty
Summary: Innate lymphoid cells (ILCs) are a rare cell population that can be divided into ILC1s, ILC2s, and ILC3s based on gene expression and cytokine production. In models of lung inflammation, ILC2s are activated by alarmins released from the epithelium, leading to increased production and migration of ILC2s within the lung. Flow cytometry is the most reliable method for identifying and characterizing ILCs due to their low abundance and lack of specific surface markers. This protocol utilizes multicolour flow cytometry to identify Lineage-Thy1.2+ ILCs and intracellular cytokine staining to specifically identify ILC2s in the lung.
Article
Biotechnology & Applied Microbiology
Iris Haberkorn, Cosima L. Off, Michael D. Besmer, Leandro Buchmann, Alexander Mathys
Summary: Microalgae are increasingly utilized in biotechnological production systems, with automated online flow cytometry (FCM) emerging as a valuable tool for optimizing microalgal bioprocesses. This technology allows real-time monitoring and assessment of key features in microbial processes, facilitating improvements in microalgal biomass production feasibility.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Oncology
Giovanni Riva, Vincenzo Nasillo, Anna Maria Ottomano, Giuliano Bergonzini, Ambra Paolini, Fabio Forghieri, Beatrice Lusenti, Patrizia Barozzi, Ivana Lagreca, Stefania Fiorcari, Silvia Martinelli, Rossana Maffei, Roberto Marasca, Leonardo Potenza, Patrizia Comoli, Rossella Manfredini, Enrico Tagliafico, Tommaso Trenti, Mario Luppi
Summary: MRD monitoring plays an increasingly important role in the clinical management of hematologic malignancies, providing valuable information for treatment selection. However, further research is needed to determine the specific prognostic value of MRD in different hematologic settings, as well as its appropriate clinical applications in order to improve standardization and harmonization of MRD monitoring protocols.
Article
Medical Laboratory Technology
Wouter H. G. Hubens, Tiago Maie, Matthis Schnitker, Ledio Bocova, Deepika Puri, Martina Wessiepe, Jan Kramer, Lothar Rink, Steffen Koschmieder, Ivan G. Costa, Wolfgang Wagner
Summary: Cell-type specific DNA methylation can be used to accurately count different leukocyte subsets in blood. In this study, the authors developed targeted DNAm assays and used digital droplet PCR to measure DNAm levels in venous blood and dried blood samples. The results showed good correlations between epigenetic estimates and conventional cell counting methods, and dried blood samples facilitated self-sampling for easier testing accessibility.
CLINICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Carolus Vitalis, Tobias Wenzel
Summary: Microfluidic droplet screens are an innovative platform for high-throughput biotechnology, contributing to advancements in discovery, product optimization, and analysis. This review focuses on the emerging trends of interaction assays in microfluidic droplets, highlighting their unique suitability. These assays encompass various biological entities and demonstrate versatility and scope. Recent advancements have expanded the scale of bioanalysis and biotechnological product design. Additionally, the review discusses pioneering breakthroughs in extending droplet-based screens into new domains, such as cargo delivery within human bodies and the development of responsive droplet structures.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Michael Bitar, Marcus Boettcher, Andreas Boldt, Fabian Hauck, Ulrike Koehl, Uwe G. Liebert, Thomas Magg, Marian S. Schulz, Ulrich Sack
Summary: The study introduced a flow cytometric-based pSTAT5A assay to quickly identify CMV-specific T cell proliferation, showing that CMV-seropositive individuals had a significant increase in expanded T cells after stimulation, unlike CMV-seronegative individuals. The correlation between pSTAT5A(+) T cells and other factors was analyzed, and immunodeficient patients with CARMIL-2 mutation showed complete deficiency in CMV-specific pSTAT5A and T cell proliferation, suggesting the potential utility of this assay in clinical practice and further validation in larger studies.
Article
Chemistry, Analytical
Chengxin Wu, Xing Wei, Xue Men, Xuan Zhang, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: CytoLM is a two-dimensional cytometry platform developed for single-particle and single-cell sampling and analysis, featuring high sensitivity and high temporal resolution. It combines VCCS-LIF and ICP-MS technologies to enable high-throughput, multi-dimensional, and multi-parameter characterization of particles and cells, showing vast potential in life science analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Jan-Stephan F. Sanders, Frederike J. Bemelman, A. Lianne Messchendorp, Carla C. Baan, Debbie van Baarle, Rob van Binnendijk, Dimitri A. Diavatopoulos, Sophie C. Frolke, Daryl Geers, Corine H. GeurtsvanKessel, Gerco den Hartog, Marieke van der Heiden, Celine Imhof, Marcia M. L. Kho, Marion P. G. Koopmans, S. Reshwan K. Malahe, Wouter B. Mattheussens, Renate van der Molen, Djenolan van Mourik, Ester B. M. Remmerswaal, Nynke Rots, Priya Vart, Rory D. de Vries, Ron T. Gansevoort, Luuk B. Hilbrands, Marlies E. J. Reinders
Summary: In kidney patients, the immunogenicity, tolerability, and safety of COVID-19 vaccination vary. Kidney transplant recipients have a poor immune response, while patients with CKD G4/5 and those on dialysis have a comparable response to the control group. Dialysis patients and transplant recipients experience fewer adverse events after vaccination.
Article
Medicine, Research & Experimental
Regina Van Zanten, Monique Van Dijk, Joost Van Rosmalen, Denise Beck, Robert Zietse, Ann Van Hecke, AnneLoes Van Staa, Emma K. Massey
Summary: The study aims to implement and test the effectiveness of the ZENN intervention in promoting self-management skills among heart, kidney, liver, and lung transplant recipients. The study will also assess the self-management support skills of nurse practitioners who will provide the intervention. This multi-centre stepped-wedge randomized controlled trial will take place from September 2020 until May 2023.
Article
Urology & Nephrology
P. Bouwmans, A. L. Messchendorp, J. S. Sanders, L. Hilbrands, M. E. J. Reinders, P. Vart, F. J. Bemelman, A. C. Abrahams, M. A. van den Dorpel, M. A. Ten Dam, A. P. J. de Vries, T. Rispens, M. Steenhuis, R. T. Gansevoort, M. H. Hemmelder
Summary: This study aims to assess the efficacy and safety of SARS-CoV-2 vaccinations in patients with CKD stages G4-G5 or on KRT. The study will observe the incidence of COVID-19 and antibody response levels after vaccination, and compare the results with a control group of non-vaccinated patients. The findings from this study will provide valuable knowledge on the effectiveness and safety of vaccination in these vulnerable patient populations.
Article
Urology & Nephrology
Jessica van der Weijden, Shekar V. K. Mahesh, Marco van Londen, Stephan J. L. Bakker, Jan-Stephan Sanders, Gerjan Navis, Robert A. Pol, Joke Roodnat, Marcia M. L. Kho, Derya Yakar, Thomas C. Kwee, Ilja M. Nolte, Stefan P. Berger, Martin H. De Borst
Summary: After living kidney donation, the glomerular filtration rate (GFR) of the remaining single kidney increases, with significant inter-individual variability. Predonation GFR, age, and kidney volume are the main determinants of the early increase in single-kidney GFR. Individuals with a greater increase in single-kidney GFR have better long-term kidney function after donation.
KIDNEY INTERNATIONAL
(2022)
Letter
Urology & Nephrology
Zainab Al Fatly, Michiel G. H. Betjes, A. Lianne Messchendorp, Jan-Stephan F. Sanders, Marlies E. J. Reinders, Marcia Mu Lan Kho, Annelies E. de Weerd
KIDNEY INTERNATIONAL REPORTS
(2022)
Article
Immunology
S. Reshwan K. Malahe, Rogier A. S. Hoek, Virgil A. S. H. Dalm, Annoek E. C. Broers, Caroline M. den Hoed, Olivier C. Manintveld, Carla C. Baan, Charlotte M. van Deuzen, Grigorios Papageorgiou, Hannelore Bax, Jeroen J. Van Kampen, Merel E. Hellemons, Marcia M. L. Kho, Rory D. de Vries, Richard Molenkamp, Marlies E. J. Reinders, Bart J. A. Rijnders
Summary: The Omicron variant of the SARS-CoV-2 virus causes less severe illness in immunocompromised patients compared to previous variants. However, hospitalization rates are still high and symptoms tend to persist for a longer duration. Additional interventions are needed to reduce the disease burden in these vulnerable individuals.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Jan Stephan F. Sanders, A. Lianne Messchendorp, Rory D. de Vries, Carla C. Baan, Debbie van Baarle, Rob van Binnendijk, Dimitri A. Diavatopoulos, Daryl Geers, Katharina S. Schmitz, Corine H. GeurtsvanKessel, Gerco den Hartog, Marcia M. L. Kho, Marion P. G. Koopmans, Renate G. van der Molen, Ester B. M. Remmerswaal, Nynke Rots, Ron T. Gansevoort, Frederike J. Bemelman, Luuk B. Hilbrands, Marlies E. J. Reinders
Summary: The immune response to COVID-19 vaccination is inferior in kidney transplant recipients and to a lesser extent in patients on dialysis or with chronic kidney disease. The antibody levels and T-cell responses significantly decrease after 6 months of vaccination, and neutralization of the Omicron variant is barely detectable.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Marcia M. L. Kho, Willem Weimar, S. Reshwan K. Malahe, Joke M. Zuijderwijk, Ronella de Kuiper, Marieken J. Boer-Verschragen, Annemiek A. van der Eijk, Dennis A. Hesselink, Marlies E. J. Reinders, Nicole M. van Besouw
Summary: This study aimed to evaluate the effect of a live attenuated VZV booster vaccine on VZV-specific immune responses in ESRD patients and healthy controls. The results showed that the VZV-specific IgG titers significantly increased after vaccination, and the percentage of memory B and T cells also increased.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Marcia M. L. Kho, A. Lianne Messchendorp, Sophie C. Froelke, Celine Imhof, Vera J. C. H. Koomen, S. Reshwan K. Malahe, Priya Vart, Daryl Geers, Rory D. de Vries, Corine H. Geurtsvan Kessel, Carla C. Baan, Renate G. van der Molen, Dimitri A. Diavatopoulos, Ester B. M. Remmerswaal, Debbie van Baarle, Rob van Binnendijk, Gerco den Hartog, Aiko P. J. de Vries, Ron T. Gansevoort, Frederike J. Bemelman, Marlies E. J. Reinders, Jan-Stephan F. Sanders, Luuk B. Hilbrands
Summary: This study aimed to compare three alternative strategies for improving the COVID-19 vaccine response in kidney transplant recipients: double vaccine dose, heterologous vaccination, and temporary discontinuation of mycophenolate mofetil or mycophenolic acid. The results showed that repeated vaccination increased SARS-CoV-2-specific antibodies in kidney transplant recipients, but higher dose, heterologous vaccine, or discontinuation of immunosuppressants did not further enhance the response.
LANCET INFECTIOUS DISEASES
(2023)
Article
Immunology
T. Tramper, D. L. Roelen, S. H. Brand-Schaaf, J. A. Kal-van Gestel, M. M. L. Kho, M. E. J. Reinders, J. I. Roodnat, J. van de Wetering, M. G. H. Betjes, A. E. de Weerd
Summary: In this retrospective analysis, the impact of donor-specific antibodies (DSA) in HLA-incompatible living donor kidney transplant recipients was studied. The results showed that recipients with DSA had inferior graft survival compared to those without DSA, and this effect was independent of the flowcytometric crossmatch, MFI value, HLA class, and origin of DSA.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Surgery
S. Reshwan K. Malahe, Yvette den Hartog, Wim J. R. Rietdijk, Debbie van Baarle, Ronella de Kuiper, Derek Reijerkerk, Alicia M. Ras, Daryl Geers, Dimitri A. Diavatopoulos, A. Lianne Messchendorp, Renate G. van der Molen, Ester B. M. Remmerswaal, Frederike J. Bemelman, Ron T. Gansevoort, Luuk B. Hilbrands, Jan-Stephan Sanders, Corine H. Geurtsvankessel, Marcia M. L. Kho, Rory D. de Vries, Marlies E. J. Reinders, Carla C. Baan
Summary: This study found that IL-21, an important regulatory factor in B cell-mediated immune response, plays a role in patients with kidney disease and kidney transplant recipients. Kidney transplant recipients showed lower levels of SARS-CoV-2-specific IL-21 producing T cells and IgG antibody levels compared to controls. The T-cell IL-21 response was positively correlated with the B cell response and IgG antibody levels. Additionally, the SARS-CoV-2-specific B cell response was shown to be dependent on IL-21.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Immunology
Yvette den Hartog, S. Reshwan K. Malahe, Wim J. R. Rietdijk, Marjolein Dieterich, Lennert Gommers, Daryl Geers, Susanne Bogers, Debbie A. van Baarle, Dimitri Diavatopoulos, A. Lianne G. Messchendorp, Renate van der Molen, Ester B. M. J. Remmerswaal, Frederike T. Bemelman, Ron B. Gansevoort, Luuk Hilbrands, Jan-Stephan H. Sanders, Corine GeurtsvanKessel, Marcia M. L. Kho, Marlies E. J. D. Reinders, Rory C. de Vries, Carla Baan
Summary: Cytokines secreted by memory T cells play a role in the immune response against SARS-CoV-2, and their expression patterns are associated with vaccine-induced immunity. A balanced production of cytokines by memory T cells is related to seroconversion and the production of antibodies.
Review
Virology
S. Reshwan K. Malahe, Jeroen J. A. van Kampen, Olivier C. C. Manintveld, Rogier A. S. Hoek, Caroline M. M. den Hoed, Carla C. C. Baan, Marcia M. L. Kho, Georges M. G. M. Verjans
Summary: Solid organ transplant recipients (SOTRs) face a high risk of human herpesvirus (HHV)-related morbidity and mortality due to immunosuppressive therapy. We aim to increase awareness of the HHV disease burden in SOTRs by providing an overview of current prevention and management strategies from the literature and guidelines. We discuss challenges in prevention, treatment, and future perspectives.
Article
Medicine, General & Internal
Sophie C. Froelke, Pim Bouwmans, A. Lianne Messchendorp, Johanna P. M. Vervoort, Alferso C. Abrahams, Aiko P. J. de Vries, Pythia T. Nieuwkerk, Marc H. Hemmelder, Ron T. Gansevoort, Luuk B. Hilbrands, Marlies E. J. Reinders, Jan-Stephan F. Sanders, Frederike J. Bemelman, Suzanne E. Geerlings, RECOVAC Collaborators
Summary: This study aimed to analyze the change in adherence to preventive measures after vaccination and awareness of antibody response in kidney transplant recipients (KTRs). The findings showed that adherence decreased after SARS-CoV-2 vaccination and in KTRs who were aware of a subsequent antibody response. However, preventive measures were effective in this vulnerable group, regardless of vaccination status.
Article
Surgery
Marry de Klerk, Judith A. Kal-van Gestel, Dave Roelen, Michiel G. H. Betjes, Annelies E. de Weerd, Marlies E. J. Reinders, Jacqueline Van de Wetering, Marcia M. L. Kho, Kristiaan Glorie, Joke I. Roodnat
Summary: Computerized integration of alternative transplantation programs (CIAT) is a kidney-exchange program that increases the chances of difficult-to-match patients by allowing AB0- and/or HLA-incompatible allocation and prioritizing highly-immunized and long waiting candidates. In a pilot study, CIAT transplanted the highest number of incompatible couples (35%), as well as a significant number of sHI patients (18%) and LW patients (20%). CIAT is a powerful addition to the limited number of programs available for difficult-to-match patients.
TRANSPLANT INTERNATIONAL
(2023)