Article
Cell Biology
Fawzia Bardag Gorce, Mais Al Dahan, Kavita Narwani, Jesus Terrazas, Monica Ferrini, Colonya C. Calhoun, Jettie Uyanne, Jun Royce-Flores, Eric Crum, Yutaka Niihara
Summary: This study reports on the isolation and expansion of neural crest stem cells (NCSCs) from the epithelium of oral mucosa (OM) using reagents that are GMP-certified and FDA-approved for clinical use. The findings suggest that NCSCs can be obtained from OM epithelium using clinical-grade reagents, which is of great significance for clinical applications in regenerative medicine.
Article
Biochemistry & Molecular Biology
Sonia Lopez, Lia Hoz, Eda Patricia Tenorio, Beatriz Buentello, Fatima Sofia Magana, Ana Wintergerst, Alejandro Navas, Yonathan Garfias, Higinio Arzate
Summary: hOMSCs derived from neural crest can self-renew, proliferate, and differentiate to multiple cell lines, showing potential for tissue engineering applications. Through culture over human amniotic membrane, hOMSCs demonstrated the capacity to differentiate toward an epithelial-like phenotype and express corneal specific markers, offering a therapeutic alternative for corneal replacement in ophthalmology field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Begona M. Bosch, Enrique Salero, Raquel Nunez-Toldra, Alfonso L. Sabater, F. J. Gil, Roman A. Perez
Summary: The failure of corneal endothelium cell monolayer is a major reason for corneal transplantation. This study proposes the use of dental pulp stem cells as an autologous cell source to reconstruct the corneal endothelium cell monolayer, bypassing ethical issues and limited clinical applications. Through a two-step differentiation protocol, dental pulp stem cells were successfully converted into corneal endothelial-like cells, demonstrating the potential for patient-derived cell therapies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Rosanna Di Tinco, Ugo Consolo, Alessandra Pisciotta, Giulia Orlandi, Giulia Bertani, Milena Nasi, Jessika Bertacchini, Gianluca Carnevale
Summary: This study evaluated the effects of three different bone substitute biomaterials (BSBs) on human dental pulp stem cells (hDPSCs). The results showed that the BSBs did not alter cell proliferation, stemness markers expression, or induce any inflammatory responses. Furthermore, the formulation of the BSBs also influenced the biological properties of the stem cells.
Article
Biology
Alexander J. Tarashansky, Jacob M. Musser, Margarita Khariton, Pengyang Li, Detlev Arendt, Stephen R. Quake, Bo Wang
Summary: SAMap is a new method that can map cell atlas manifolds across species within phyla, identifying homologous cell types with shared expression programs, even in complex examples where homologous tissues emerge from distinct germ layers.
Article
Cell & Tissue Engineering
Qunzhou Zhang, Phuong Nguyen, Justin C. Burrell, Jincheng Zeng, Shihong Shi, Rabie M. Shanti, Grace Kulischak, D. Kacy Cullen, Anh D. Le
Summary: This study identified a rapid biofabrication approach for functionalized NGCs by directing GMSCs conversion into GiSCs in 3D collagen hydrogel. The implantation of functionalized NGCs laden with GiSCs significantly improved functional recovery and axonal regeneration in a segmental facial nerve defect model in rats.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Multidisciplinary Sciences
Zachary T. Olmsted, Janet L. Paluh
Summary: Researchers have successfully studied the co-development of the central and peripheral nervous systems with trunk mesendoderm in elongating multi-lineage organized (EMLO) gastruloids. They identified NCCs that differentiate to form peripheral neurons integrated with an upstream spinal cord region after day 8.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Arseniy Lobov, Polina Kuchur, Aigul Khizhina, Anastasia Kotova, Andrei Ivashkin, Daria Kostina, Polina Klausen, Evgeniia Khokhlova, Egor Repkin, Kseniia Postnikova, Daria Perepletchikova, Evgeny Denisov, Tatiana Gerashchenko, Rashid Tikhilov, Svetlana Bozhkova, Andrey Sereda, Vitaliy Karelkin, Natella Enukashvily, Anna Malashicheva
Summary: In this study, we conducted a comparative analysis of mesenchymal cell primary cultures from various sources and found differences in their ability to undergo osteogenic differentiation and produce extracellular matrix. Based on the analysis of secretome, proteome, and transcriptome, we classified them into dental stem cells, mesenchymal stem cells, and fetal stem cells. Although they have differences, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. These findings shed light on the distinctive characteristics of mesenchymal stem cells and provide insights into their potential applications in regenerative medicine and the inherent diversity of mesenchymal cells in vivo.
Article
Biochemistry & Molecular Biology
Leah C. Ott, Christopher Y. Han, Jessica L. Mueller, Ahmed A. Rahman, Ryo Hotta, Allan M. Goldstein, Rhian Stavely
Summary: Neurons and glia of the peripheral nervous system are derived from progenitor cell populations originating from embryonic neural crest, and these cells have previously been shown to possess neural stem cell qualities. In this study, a population of neural crest-derived Schwann cells residing in the bone marrow, in association with nerve fibers, were found to exhibit plasticity and generate neural stem cells with neurogenic potential. These cells can form neural networks within the enteric nervous system following transplantation, making them a novel source of autologous neural stem cells for the treatment of neurointestinal disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Sarah Janice Hoerner, Nathalie Couturier, Daniele Caroline Gueiber, Mathias Hafner, Ruediger Rudolf
Summary: This article provides an overview of the current resources and methods for the differentiation of Schwann cells, supports the comparison and refinement of protocols, and aids the choice of suitable methods for specific applications.
Review
Cell Biology
Anna L. Hoeving, Beatrice A. Windmoeller, Cornelius Knabbe, Barbara Kaltschmidt, Christian Kaltschmidt, Johannes F. W. Greiner
Summary: The review highlights the cellular heterogeneity within neural crest-derived stem cell populations and emphasizes this heterogeneity as a crucial parameter for understanding their role in tissue homeostasis and regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Retraction
Biochemistry & Molecular Biology
Sabine Wislet-Gendebien, Emerence Laudet, Virginie Neirinckx, Philippe Alix, Pierre Leprince, Aneta Glejzer, Christophe Poulet, Benoit Hennuy, Lukas Sommer, Olga Shakhova, Bernard Rogister
Summary: A correction to this paper has been published.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Shoichiro Tani, Hiroyuki Okada, Ung-il Chung, Shinsuke Ohba, Hironori Hojo
Summary: Skeletal disorders, such as osteoarthritis and bone fractures, can greatly impact the quality of life for elderly individuals. Regenerative therapies using skeletal cells are a promising treatment option, with two main strategies for cell sources: induced pluripotent stem cells or extraction from skeletal tissues. These cells have the potential for differentiation into various skeletal cell types and offer applications for skeletal regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Kyung-Joo Seong, Seungho Choi, Hyun-Gwan Lee, Joon Haeng Rhee, Jin Ho Lee, Jeong-Tae Koh, Sun-Hun Kim, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Summary: Toll-like receptor 5 plays a crucial role in regulating neurogenesis from mouse embryonic stem cells and adult hippocampal neural stem cells, promoting neural differentiation and inhibiting cell proliferation. TLR5 deficiency impairs fear memory performance in mice.
Article
Surgery
Chihiro Yoshida, Satoshi Yamaguchi, Shigehiro Abe, Kiyoshi Harada
JOURNAL OF CRANIOFACIAL SURGERY
(2016)
Article
Dentistry, Oral Surgery & Medicine
S. Ishii, S. Abe, A. Moro, N. Yokomizo, Y. Kobayashi
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
(2017)
Article
Biochemistry & Molecular Biology
Shigehiro Abe, Satoshi Yamaguchi, Akihiko Watanabe, Keiichi Hamada, Teruo Amagasa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2008)
Article
Cell Biology
Shigehiro Abe, Keiichi Hamada, Masahiko Miura, Satoshi Yamaguchi
CELL BIOLOGY INTERNATIONAL
(2012)
Article
Cell & Tissue Engineering
Shigehiro Abe, Keiichi Hamada, Satoshi Yamaguchi, Teruo Amagasa, Masahiko Miura
STEM CELL RESEARCH & THERAPY
(2011)
Article
Cell Biology
Shigehiro Abe, Atsushi Kaida, Kazunori Kanemaru, Keiichiro Nakazato, Naoko Yokomizo, Yutaka Kobayashi, Masahiko Miura, Toshio Miki, Chiaki Hidai, Hisataka Kitano, Tetsuya Yoda
Summary: Although multilineage cells derived from different oral tissues share similar properties, they exhibit differences in the expression of certain markers and differentiation abilities.
CELL PROLIFERATION
(2022)
Article
Dentistry, Oral Surgery & Medicine
Tatsuro Kobayashi, Shigehiro Abe, Naoko Yokomizo, Hisataka Kitano, Takashi Ono, Toshiaki Takeda, Yutaka Kobayashi
Summary: The study utilized 3D-CT images to evaluate mandibular condyle fractures and found that morphometric measurements could help predict malocclusion after closed treatment.
JOURNAL OF ORAL SCIENCE
(2021)
Correction
Multidisciplinary Sciences
Ayumi Moro, Shigehiro Abe, Naoko Yokomizo, Yutaka Kobayashi, Takashi Ono, Toshiaki Takeda
Article
Surgery
Shigehiro Abe, Naoko Yokomizo, Yutaka Kobayashi, Kouhei Yamamoto
INTERNATIONAL JOURNAL OF SURGERY CASE REPORTS
(2015)
Article
Dentistry, Oral Surgery & Medicine
Shigehiro Abe, Satoshi Yamaguchi, Teruo Amagasa
ORAL SCIENCE INTERNATIONAL
(2007)
Article
Multidisciplinary Sciences
Ayumi Moro, Shigehiro Abe, Naoko Yokomizo, Yutaka Kobayashi, Takashi Ono, Toshiaki Takeda