Article
Biochemistry & Molecular Biology
Wolfgang Winnicki, Andreas Fichtenbaum, Harald Herkner, Florina Regele, Michael Baier, Sieglinde Zelzer, Ludwig Wagner, Guerkan Sengoelge
Summary: This study aimed to investigate individualized approaches to mycophenolic acid (MPA) therapy to improve clinical outcomes in renal transplant patients. The results showed that pharmacodynamic monitoring of IMPDH activity in peripheral blood cells may be an effective method for drug dose adjustment.
Letter
Clinical Neurology
Pin-Shiuan Chen, Meng-Chen Wu, Chun-Hwei Tai, Yung-Yee Chang, Min-Yu Lan, Ying-Fa Chen, Han- Lin, Ni-Chung Lee, Chin-Hsien Lin
Summary: We investigated 245 Taiwanese patients with dystonia and found no evidence to suggest a link between the inosine monophosphate dehydrogenase gene (IMPDH2) and the disease, suggesting that IMPDH2 may not play a major role in dystonia.
PARKINSONISM & RELATED DISORDERS
(2023)
Review
Biochemistry & Molecular Biology
David W. Wolff, Anna Bianchi-Smiraglia, Mikhail A. Nikiforov
Summary: Inhibition of enzymes involved in GTP biosynthesis has significant effects on biological processes. However, our understanding of the regulation of GTP metabolism and its role in cellular processes is incomplete. Novel methodologies have provided insights into the distribution and function of GTP molecules. This review analyzes recent discoveries in GTP metabolism and evaluates key enzymes as molecular targets.
TRENDS IN MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Takao Morimune, Ayami Tano, Yuya Tanaka, Haruka Yukiue, Takefumi Yamamoto, Ikuo Tooyama, Yoshihiro Maruo, Masaki Nishimura, Masaki Mori
Summary: Research has shown that the protein Tbc1d24 forms unique cellular structures called cytoophidia in neuronal cells, which are different from other types of cytoophidia. The formation of Tbc1d24 cytoophidia is influenced by cellular juvenescence and affects its enzymatic activity.
Article
Chemistry, Organic
Xin Liu, Yan Fu, Qiqi Zhou, Su Wang, Lu Gao, Jiali Lei, Aibing Ke, Yeying Li, Xuexia Zhang, Changhong Huo, Xinhua Lu
Summary: Five unprecedented chromone derivatives were obtained from the fungus Aspergillus deflectus NCC0415, and their structures were identified using various techniques. The hexacyclic derivatives showed potent enzyme inhibition activity.
Article
Agriculture, Multidisciplinary
Yun Wang, Tianqing Xia, Chenhao Li, Duwen Zeng, Lili Xu, Liyun Song, Hengsong Yu, Shichao Chen, Jianzhi Zhao, Xiaoming Bao
Summary: Saccharomyces cerevisiae has been identified as a preferred source for industrial production of ribonucleic acids (RNAs) and their derivatives. In this study, a modified RNA polymerase I-mediated green fluorescent protein expression system was used to screen and identify an industrial S. cerevisiae strain with an 18.2% increase in RNA content. Transcriptome analysis showed enhanced activity of genes and pathways associated with rRNA transcription, purine metabolism, and phosphate transport. The study provides valuable insights into RNA synthesis and its application in the food industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Anika L. Burrell, Justin M. Kollman
Summary: Inosine-5'-monophosphate dehydrogenase (IMPDH) is an important enzyme in purine metabolism, regulating flux at the branch point between adenine and guanine nucleotide synthesis and participating in transcription regulation. Mutations in IMPDH1 and IMPDH2 are associated with different human diseases, but their effects at the enzyme level are still unclear. Mutations in IMPDH1 lead to retinal degeneration and recent studies have revealed the molecular mechanisms behind the functional defects. IMPDH1 splice variants in the retina up-regulate guanine nucleotide synthesis by reducing sensitivity to feedback inhibition by downstream products. Understanding the role of IMPDH1 in the retina and characterizing an animal disease model will provide insights into the molecular mechanism of IMPDH1-associated blindness.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Chemistry, Medicinal
Ling-Jie Gao, Yuan Lin, Steven De Jonghe, Mark Waer, Piet Herdewijn
Summary: Researchers prepared three different types of prodrugs of Mizoribine, and found that the ester derivatives effectively inhibited the secretion of specific immune molecules and prolonged graft survival. The combination therapy of these prodrugs with tacrolimus also showed a synergistic effect in vivo.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Marianna Lovaszi, Zoltan H. Nemeth, William C. Gause, Jennet Beesley, Pal Pacher, Gyorgy Hasko
Summary: The study demonstrates that IMP can regulate inflammation through metabolism to inosine, which may be beneficial for treating certain diseases clinically.
Article
Oncology
Shiyu Wang, Fan Chao, Cong Zhang, Dunsheng Han, Guoxiong Xu, Gang Chen
Summary: The study found that circPFKP was significantly increased in prostate cancer tissues compared to noncancerous tissues, and was correlated with PCa risk classification, N stage, and prognostic group. CircPFKP promotes PCa cell proliferation by interacting with IMPDH2, and its production may be facilitated by hnRNPF binding.
Article
Food Science & Technology
Yusa Nakamura, Takanori Sato, Muneshige Takatori, Toshihiko Hirama, Kazuhito Oshima, Kigen Takahashi
Summary: This study provides the first report on the effective utilization of the deep sea for improving food quality. The results suggest that deep-sea aging can promote proteolysis and enhance the quality of fish such as greater amberjack, while its effectiveness may vary depending on fish species and depths.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Medical Laboratory Technology
Xiaoyuan Jia, Yao Liu, Yan Cheng, Yin Wang, Hui Kang, Zhongren Ma, Kan Chen
Summary: This study reveals the role of IMPDH1 in promoting HCC growth and progression, and its association with patient cumulative survival.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Physiology
Baojun Yu, Zhengyun Cai, Jiamin Liu, Wei Zhao, Xi Fu, Yaling Gu, Juan Zhang
Summary: Through transcriptome analysis, this study identified several genes, such as PDHA2, ACSS2, PGAM1, GAPDH, PGM1, GPI, and TPI1, that may be involved in the deposition of inosine monophosphate (IMP) in muscle. Functional enrichment analysis revealed the regulatory pathways of PYCR1, SMOX, and ACSS2 in glycolysis/gluconeogenesis, arginine and proline metabolism, and pyruvate metabolism. Additionally, TGIF1 and THBS1 were identified as potential candidate genes affecting IMP deposition in muscle.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anika L. Burrell, Chuankai Nie, Meerit Said, Jacqueline C. Simonet, David Fernandez-Justel, Matthew C. Johnson, Joel Quispe, Ruben M. Buey, Jeffrey R. Peterson, Justin M. Kollman
Summary: A series of cryo-EM structures of human IMPDH1 variants reveal polymorphic filaments. Blindness-associated mutations in IMPDH1 are characterized and half disrupt feedback inhibition. These findings provide a foundation for understanding the role of IMPDH1 in retinal function and disease.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Biochemical Research Methods
Tobias Tekath, Martin Dugas
Summary: DTUrtle is a software package for analyzing bulk and single-cell RNA-seq datasets, allowing for transcript-level differential analysis with various result aggregation, visualization options, and a novel detection probability score. Successfully applied to human and mouse data, potential applications include identifying cell-type specific transcript isoforms.
Letter
Surgery
Ashwin Rammohan, Mettu S. Reddy, Pal-Dag Line, Mohamed Rela
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Pediatrics
Anniken B. Ostensen, Anne-Britt Skarbo, Truls Sanengen, Pal-Dag Line, Runar Almaas
Summary: This study assessed the longitudinal neurocognitive development after liver transplantation and identified factors associated with neurocognitive performance. The study found that young age at transplantation and large blood transfusions during transplantation were risk factors for poor neurocognitive performance later in life. Cognitive performance did not improve over time after transplantation.
JOURNAL OF PEDIATRICS
(2022)
Article
Gastroenterology & Hepatology
Gisle Kjosen, Rune Horneland, Espen Nordheim, Einar Martin Aandahl, Pal-Dag Line, Kristina Rydenfelt, Trond Geir Jenssen, Tor Inge Tonnessen, Hakon Haugaa
Summary: This study validated the Pancreas Donor Risk Index (PDRI) in a Norwegian population and found that it was unable to predict 1-year graft survival. Significant differences were also observed between the Norwegian and US populations.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Immunology
Magdalena Salcedo, Carmen Vinaixa, Milind Javle, Maria Trapero-Marugan, Javier Bustamante, Pal-Dag Line
Summary: This international consensus document discusses the evaluation, impact of treatment, and surveillance of posttransplant cancer recurrence in patients with prior nonhepatic cancer undergoing liver transplantation. It emphasizes the importance of further research and consensus guidelines.
Review
Immunology
Margot Fodor, Heinz Zoller, Rupert Oberhuber, Robert Sucher, Daniel Seehofer, Umberto Cillo, Pal Dag Line, Herbert Tilg, Stefan Schneeberger
Summary: In recent years, improvements in liver transplant survival rates have increased access to transplantation for higher-risk patients and spurred strategies to rescue high-risk donor organs, prompting a reevaluation of assessment endpoints for liver transplant therapies.
Article
Gastroenterology & Hepatology
Harald Grut, Pal-Dag Line, Knut J. Labori, Anselm Schulz, Svein Dueland
Summary: This study compared the survival outcomes of resection and liver transplantation in patients with moderate metabolic tumor volume (MTV) in colorectal liver metastases. The results showed that liver transplantation had significantly higher overall survival and post-recurrence survival compared to resection.
Article
Gastroenterology & Hepatology
Espen Lindholm, Gisli Bjorn Bergmann, Hakon Haugaa, Knut Jorgen Labori, Sheraz Yaqub, Bjorn Atle Bjornbeth, Pal-Dag Line, Guro Grindheim, Gisle Kjosen, Soren Erik Pischke, Tor Inge Tonnessen
Summary: This study found that placing microdialysis catheters near the pancreaticojejunostomy can detect the occurrence of pancreatic fistulas earlier. The elevated glycerol levels in the microdialysate during the first postoperative samples had high sensitivity and specificity in detecting pancreatic fistulas. Subsequent inflammation was detected by increased lactate levels, lactate-to-pyruvate ratio, and decreased glucose levels.
Article
Gastroenterology & Hepatology
Ernesto Sparrelid, Trygve Thorsen, Christina Sauter, Carl Jorns, Per Stal, Arno Nordin, Marieke T. de Boer, Carlijn Buis, Sheraz Yaqub, Nicolai A. Schultz, Peter N. Larsen, Ville Sallinen, Pal-Dag Line, Stefan Gilg
Summary: This article describes the clinical experience of five northern European tertiary centers in using liver transplantation (LTX) to treat patients with severe, intractable post-hepatectomy liver failure (PHLF). The study found that LTX can be a life-saving procedure with low short-term risk in selected patients with PHLF.
Letter
Surgery
Svein Dueland, Sheraz Yaqub, Pal-Dag Line
Article
Gastroenterology & Hepatology
Svanhildur Haflidadottir, Anniken Bjornstad Ostensen, Iren Lindbak Matthews, Pal-Dag Line, Runar Almaas
Summary: The risk factors for food allergies in children following liver transplantation are still unknown. Use of MMF after transplantation was associated with lower rates of food allergy and sensitization against food allergens, and this effect was not due to reduced trough levels of tacrolimus.
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2022)
Article
Multidisciplinary Sciences
Jie Hou, Olav Magnus Ivar Liavag, Ida Hoy Faerden, Orjan Grottem Martinsen, Tor Inge Tonnessen, Pal-Dag Line, Morten Hagness, Jan Olav Hogetveit, Soren Erik Pischke, Runar Strand-Amundsen
Summary: There is a global shortage of donor livers, leading to the death of patients on waiting lists. Researchers have assessed the condition of porcine livers by monitoring their microwave dielectric properties, aiming to expand the pool of available donor livers. The study found that dielectric relaxation spectroscopy can be used to detect and differentiate liver injuries, providing a standardized and reliable evaluation method for livers prior to transplantation.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Svein Dueland, Tor Magnus Smedman, Harald Grut, Trygve Syversveen, Lars Hilmar Jorgensen, Pal-Dag Line
Summary: Many colorectal cancer patients with liver-only metastases who receive liver transplantation develop pulmonary metastases after the transplantation. The pre-transplant PET liver uptake determines the overall survival in patients treated by post-transplant resection of pulmonary metastases. The study aims to determine the impact of PET uptake on liver metastases on overall survival after resection of pulmonary metastases in patients who had received liver transplantation due to unresectable colorectal liver-only metastases.
Editorial Material
Gastroenterology & Hepatology
Ina Marie Andersen, Henrik M. Reims, Krzyztof Grzyb, Borre Fevang, Espen Melum, Pal Aukrust, Kristine Wiencke, Ingvild Nordoy, Pal-Dag Line, Silje Fjellgard Jorgensen
LIVER TRANSPLANTATION
(2023)
Article
Surgery
Tor Magnus Smedman, Tormod Kyrre Guren, Kjell Magne Tveit, Maria Thomsen, Marit Helen Andersen, Pal-Dag Line, Svein Dueland
Summary: This study describes the HRQoL in patients with non-resectable CRLM receiving LT and investigates the impact of baseline HRQL factors on overall survival. The study found that appetite loss and pain at baseline had a negative impact on survival, but the overall health-related quality of life remained good after transplantation.
TRANSPLANT INTERNATIONAL
(2022)
Letter
Surgery
Svein Dueland, Sheraz Yaqub, Pal-Dag Line