Article
Medicine, Research & Experimental
Aaditya Khatri, Jamie L. . Todd, Fran L. . Kelly, Andrew Nagler, Zhicheng Ji, Vaibhav Jain, Simon G. Gregory, Kent J. Weinhold, Scott M. Palmer
Summary: Chronic lung allograft dysfunction (CLAD), characterized by decline in pulmonary function and bronchiolitis obliterans, lacks approved therapies. Single-cell RNA-Seq and spatial transcriptomic analysis identified the important role of JAK-STAT signaling in airway epithelial cells in CLAD. Activation of JAK-STAT signaling leads to upregulation of MHC-I in airway basal cells, resulting in cytotoxic T cell-mediated basal cell death. This study provides mechanistic insight and potential therapeutic strategy for CLAD.
Article
Hematology
Yifan Pang, Ananth Charya, Michael B. Keller, Arlene Sirajuddin, Yi-Ping Fu, Noa G. Holtzman, Steven Z. Pavletic, Sean Agbor-Enoh
Summary: This study adapted the 2019 International Society for Heart and Lung Transplantation criteria to define novel phenotypes of pulmonary chronic graft-versus-host disease (PcGVHD) and compared its performance with the NIH criteria in identifying high-risk PcGVHD patients. The adapted criteria were found to be effective in identifying high-risk PcGVHD patients that were missed by the NIH criteria.
Article
Critical Care Medicine
Jarrod E. Dalton, Carli J. Lehr, Paul R. Gunsalus, Lyla Mourany, Maryam Valapour
Summary: This study examined the performance of models predicting waitlist and posttransplant survival in lung transplant allocation. The results showed that different models had similar performance in predicting waitlist and posttransplant survival, and using alternative modeling strategies and contemporary cohorts did not improve the models' performance in determining access to lung transplant.
Article
Medicine, General & Internal
Jesper Romhild Davidsen, Christian B. Laursen, Mikkel Hojlund, Thomas Kromann Lund, Klaus Nielsen Jeschke, Martin Iversen, Anna Kalhauge, Elisabeth Bendstrup, Jorn Carlsen, Michael Perch, Daniel Pilsgaard Henriksen, Hans Henrik Lawaetz Schultz
Summary: This study found that pleural thickening identified by lung ultrasound may serve as a potential surrogate marker to distinguish restrictive allograft syndrome (RAS) from bronchiolitis obliterans syndrome (BOS) in chronic lung allograft dysfunction (CLAD) patients after lung transplant. This discovery provides a new method for clinical diagnosis of CLAD patients post lung transplant.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Medicine, General & Internal
Christophe Pison, Adrien Tissot, Eric Bernasconi, Pierre-Joseph Royer, Antoine Roux, Angela Koutsokera, Benjamin Coiffard, Benjamin Renaud-Picard, Jerome Le Pavec, Pierre Mordant, Xavier Demant, Thomas Villeneuve, Jean-Francois Mornex, Simona Nemska, Nelly Frossard, Olivier Brugiere, Valerie J. Siroux, Benjamin Marsland, Aurore Foureau, Karine Botturi, Eugenie Durand, Johann Pellet, Richard Danger, Charles Auffray, Sophie Brouard, Laurent Nicod, Antoine Magnan
Summary: SysCLAD is a research aiming to predict chronic lung allograft dysfunction (CLAD), which involves investigating various factors including clinical data, assessment of airway microbiota, air pollution studies, activation of immune cells, secretion of matrix metalloproteinase-9 by T cells, subpopulations of T and B cells, genome polymorphisms, blood transcriptome, plasma proteome studies, and MSK1 expression to develop predictors of CLAD.
FRONTIERS IN MEDICINE
(2023)
Article
Respiratory System
Jesper M. Magnusson, Petrea Ericson, Sara Tengvall, Marit Stockfelt, Bettina Brundin, Anders Linden, Gerdt C. Riise
Summary: This pilot study revealed a significant increase in extracellular IL-26 protein concentration in BAL samples from patients with BOS, but not in samples from patients with AR. Macrophages and lymphocytes were identified as cellular sources of IL-26 protein in BAL cells obtained from BOS patients.
RESPIRATORY RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Wai Wong, Brandy Johnson, Pi Chun Cheng, Maureen B. Josephson, Katsuhide Maeda, Robert A. Berg, Steven M. Kawut, Michael O. Harhay, Samuel B. Goldfarb, Nadir Yehya, Adam S. Himebauch
Summary: This study aimed to investigate the impact of grade 3 PGD within 48 or 72 hours on CLAD-free survival following pediatric lung transplantation. The study found that the occurrence of grade 3 PGD within 48 or 72 hours was associated with shorter CLAD-free survival, and gender may be a modifier of this association.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Respiratory System
Micheal C. McInnis, Jin Ma, Gauri Rani Karur, Christian Houbois, Liran Levy, Jan Havlin, Eyal Fuchs, Jussi Tikkanen, Chung-Wai Chow, Ella Huszti, Tereza Martinu
Summary: In this study, a machine learning computed tomography (CT) lung texture analysis tool was used for phenotyping and prognostication of chronic lung allograft dysfunction (CLAD) in lung transplant recipients. The results showed that machine learning could effectively distinguish different CLAD phenotypes, and the associated parameters were able to predict the risk of graft failure.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Cell Biology
Laura Bergantini, Miriana d'Alessandro, Elda De Vita, Felice Perillo, Antonella Fossi, Luca Luzzi, Piero Paladini, Anna Perrone, Paola Rottoli, Piersante Sestini, Elena Bargagli, David Bennett
Summary: In acute rejection (AR) and chronic lung allograft dysfunction, the balance between immune cells is different, with a predominance of Th17 cells subtypes and depletion of Tregs observed in AR patients. Understanding the balance between regulatory and effector cells can effectively discriminate patients' immunological status.
Review
Respiratory System
Bahaa Bedair, Ramsey R. Hachem
Summary: CLAD is a chronic condition that limits outcomes after lung transplantation, with bronchiolitis obliterans syndrome being more extensively studied for diagnosis and treatment than restrictive allograft syndrome. The current evidence mainly supports the use of azithromycin in treating CLAD, while other therapies have limited data and are supported by case studies and observational research. Retransplantation may also be considered for CLAD management.
JOURNAL OF THORACIC DISEASE
(2021)
Article
Surgery
Naofumi Miyahara, Alberto Benazzo, Felicitas Oberndorfer, Akinori Iwasaki, Viktoria Laszlo, Balasz Dome, Mir Ali Hoda, Peter Jaksch, Walter Klepetko, Konrad Hoetzenecker
Summary: miR-21 expression was higher in restrictive allograft syndrome (RAS) than in bronchiolitis obliterans syndrome (BOS) specimens, with a significant increase in serum concentration over time in RAS patients. Vimentin, NICD, and p-SMAD 2/3 were positive in a higher proportion of BOS patients compared to RAS cases.
TRANSPLANT INTERNATIONAL
(2022)
Review
Medicine, Research & Experimental
Aparna C. Swaminathan, Jamie L. Todd, Scott M. Palmer
Summary: Lung transplantation has substantially increased in volume in recent decades, with advances being made in understanding and treating post-transplant complications like CLAD. Ongoing studies are exploring novel approaches to improve long-term outcomes for lung transplant recipients.
ANNUAL REVIEW OF MEDICINE, VOL 72, 2021
(2021)
Article
Immunology
Juan Margallo Iribarnegaray, Alicia De Pablo Gafas, Rodrigo Alonso Moralejo, Carlos Andres Quezada Loaiza, Fernando Revuelta Salgado, Isabel Pina Maiquez, Virginia Luz Perez Gonzalez
Summary: Among 13 CLAD patients treated with ATG, half of them showed a favorable positive response, with most responders being in CLAD stage 1 to 2. Lung function improved after treatment. The study also found a trend toward better survival in early CLAD patients.
TRANSPLANTATION PROCEEDINGS
(2021)
Review
Surgery
Rickey A. Evans, Krysta S. Walter, Leonard Jason Lobo, Raymond Coakley, Christina T. Doligalski
Summary: Chronic lung allograft dysfunction (CLAD) remains the leading cause of death in lung transplant recipients (LTRs) despite improvements in immunosuppression management. Treatments for CLAD are usually ineffective and there are currently no evidence-based guidelines.
CLINICAL TRANSPLANTATION
(2022)
Review
Respiratory System
Sara Assadiasl, Mohammad Hossein Nicknam
Summary: Post-transplant care and patients' survival in lung transplantation need improvement, and cytokine monitoring pre- and post-transplant could be used for prevention and early diagnosis of inflammatory events, providing new therapeutic options to enhance lung allograft survival.
Editorial Material
Cardiac & Cardiovascular Systems
Dirk Van Raemdonck, Laurens J. Ceulemans, Robin Vos, Geert M. Verleden
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Editorial Material
Surgery
Michaela Orlitova, Marc Gewillig, Jan Van Slambrouck, Dirk Vlasselaers, Bart Jacobs, Arne P. Neyrinck, Lieven Depypere, Laurent Godinas, Robin Vos, Geert M. Verleden, Dirk E. Van Raemdonck, Laurens J. Ceulemans
Summary: Pulmonary vein stenosis (PVS) and pulmonary vein occlusion (PVO) are rare complications after lung transplantation (LTx) with limited treatment options and a high risk of graft loss. We describe two cases of successful endovascular transatrial stenting following double LTx. Both patients, one with chronic obstructive pulmonary disease and the other with idiopathic pulmonary arterial hypertension, underwent endovascular transatrial dilatation and stenting to treat PVS and PVO respectively, avoiding the need for surgical reintervention. This treatment option appears to be effective and safe for these life-threatening complications after LTx.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Oncology
Tom Vandaele, Jan Van Slambrouck, Viktor Proesmans, Paul Clement, Maarten Lambrecht, Philippe Nafteux, Dirk Van Raemdonck, Laurens J. Ceulemans
Summary: This review provides an overview of the current literature on hyperthermic intrathoracic chemotherapy (HITHOC) for thymoma with pleural dissemination (TPD). The results suggest that combining cytoreductive surgery and HITHOC is feasible and safe for TPD, but there is significant heterogeneity in the literature, calling for further research to better understand the additional benefit of HITHOC in TPD treatment.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Editorial Material
Oncology
Tom Vandaele, Jan Van Slambrouck, Viktor Proesmans, Paul Clement, Maarten Lambrecht, Philippe Nafteux, Dirk Van Raemdonck, Laurens J. Ceulemans
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Laura P. M. H. de Rooij, Lisa M. Becker, Laure Anne Teuwen, Bram Boeckx, Sander Jansen, Simon Feys, Stijn Verleden, Laurens Liesenborghs, Anna K. Stalder, Sasha Libbrecht, Tina Van Buyten, Gino Philips, Abhishek Subramanian, Sebastien J. Dumas, Elda Meta, Mila Borri, Liliana Sokol, Amelie Dendooven, Anh Co K. Truong, Jan Gunst, Pierre Van Mol, Jasmin D. Haslbauer, Katerina Rohlenova, Thomas Menter, Robbert Boudewijns, Vincent Geldhof, Stefan Vinckier, Jacob Amersfoort, Wim Wuyts, Dirk Van Raemdonck, Werner Jacobs, Laurens J. Ceulemans, Birgit Weynand, Bernard Thienpont, Martin Lammens, Mark Kuehnel, Guy Eelen, Mieke Dewerchin, Luc Schoonjans, Danny Jonigk, Jo van Dorpe, Alexandar Tzankov, Els Wauters, Massimiliano Mazzone, Johan Neyts, Joost Wauters, Diether Lambrechts, Peter Carmeliet
Summary: This study provides new insights into the abundance, expression patterns, and interactomes of endothelial cell (EC) subtypes in COVID-19 and idiopathic pulmonary fibrosis (IPF). The findings suggest the importance of ECs in the pathophysiology of both conditions and may contribute to future investigations into the progression and treatment of COVID-19 and IPF.
CARDIOVASCULAR RESEARCH
(2023)
Article
Surgery
Cedric Vanluyten, Christelle M. Vandervelde, Robin Vos, Jan Van Slambrouck, Steffen Fieuws, Paul De Leyn, Philippe Nafteux, Herbert Decaluwe, Hans Van Veer, Lieven Depypere, Yanina Jansen, An-Lies Provoost, Arne P. Neyrinck, Catherine Ingels, Bart M. Vanaudenaerde, Laurent Godinas, Lieven J. Dupont, Geert M. Verleden, Dirk Van Raemdonck, Laurens J. Ceulemans
Summary: This study retrospectively analyzed single-center bilateral lung transplantations performed between 2010 and 2020. The results showed that lung transplantation from donors aged ≥70 years is feasible and safe, with comparable short and long-term outcomes to transplantation from younger donors.
Review
Pharmacology & Pharmacy
Saskia Bos, Pauline Pradere, Hanne Beeckmans, Andrea Zajacova, Bart M. Vanaudenaerde, Andrew J. Fisher, Robin Vos
Summary: Chronic lung rejection, also known as chronic lung allograft dysfunction (CLAD), is a major obstacle for long-term survival after lung transplantation. Limited therapeutic options are available, but lymphocyte depleting and immunomodulating therapies such as extracorporeal photopheresis, anti-thymocyte globulin, and total lymphoid irradiation appear to be the most effective treatments for progressive CLAD patients, considering both efficacy and risk of side effects.
PHARMACOLOGICAL REVIEWS
(2023)
Article
Respiratory System
Janne Goossens, Anne-Charlotte Jonckheere, Sven F. Seys, Ellen Dilissen, Tatjana Decaesteker, Camille Goossens, Koen Peers, Vincent Vanbelle, Jeroen Stappers, Sven Aertgeerts, Barbara De Wilde, Jasmine Leus, Sophie Verelst, Marc Raes, Lieven Dupont, Dominique M. Bullens
Summary: The study investigated the effects of intense exercise and air pollution exposure on the airway barrier and exercise-induced bronchoconstriction (EIB) in adolescent elite athletes. Results showed that athletes had increased markers of air pollution exposure, epithelial damage, and airway inflammation compared with controls. The incidence of EIB in athletes was also linked to prior exposure to PM10 and PM2.5.
Article
Physiology
Thibaux Van der Stede, Jan Spaas, Sarah de Jager, Jana De Brandt, Camilla Hansen, Jan Stautemas, Bjarne Vercammen, Siegrid De Baere, Siska Croubels, Charles-Henri Van Assche, Berta Cillero Pastor, Michiel Vandenbosch, Ruud Van Thienen, Kenneth Verboven, Dominique Hansen, Thierry Bove, Bruno Lapauw, Charles Van Praet, Karel Decaestecker, Bart Vanaudenaerde, Bert O. Eijnde, Lasse Gliemann, Ylva Hellsten, Wim Derave
Summary: This study systematically analyzed the distribution and metabolism of histidine-containing dipeptides (HCDs) in mouse, rat, and human tissues using a sensitive UHPLC-MS/MS approach. The results show that tissue HCD levels are uniquely produced by carnosine synthase (CARNS1) in fast-twitch skeletal muscle fibers and brain oligodendrocytes. Additionally, N-acetylcarnosine is a stable plasma HCD and is enriched in human skeletal muscles.
Article
Physiology
Michaela Orlitova, Tom Verbelen, Anna E. Frick, Arno Vanstapel, Dieter Van Beersel, Sofie Ordies, Jan Van Slambrouck, Janne Kaes, Xin Jin, Walter Coudyzer, Stijn E. Verleden, Geert M. Verleden, Bart M. Vanaudenaerde, Dirk E. Van Raemdonck, Robin Vos, Laurens J. Ceulemans, Piet Claus, Arne P. Neyrinck
Summary: Lung transplantation is a challenging procedure and can result in severe damage to the transplanted pulmonary graft. The interaction between right ventricular function and lung injury during the transplantation process is poorly understood. Through a translational porcine model, it was found that right ventricular failure can occur as a result of forcing the entire cardiac output through a lung suffering from ischemia-reperfusion injury. The study also revealed a delicate balance between the development of pulmonary ischemia-reperfusion injury and right ventricular function, highlighting the potential benefits of extracorporeal life support technology in these cases.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Seppe Cambier, Fabio Beretta, Noemie Portner, Mieke Metzemaekers, Ana Carolina de Carvalho, Erik Martens, Janne Kaes, Celine Aelbrecht, Cato Jacobs, Pierre Van Mol, Els Wauters, Philippe Meersseman, Greet Hermans, Rafael Elias Marques, Bart Vanaudenaerde, Robin Vos, Joost Wauters, Mieke Gouwy, Paul Proost
Summary: CXCL12, which is present in the lungs and circulation of COVID-19 patients, undergoes rapid proteolytic processing and degradation. The proteolysis is mainly mediated by neutrophil elastase in COVID-19 BAL fluids with high proteolytic activity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Respiratory System
Vincent Geudens, Jan Van Slambrouck, Gitte Aerts, Lynn Willems, Tinne Goos, Janne Kaes, Andrea Zajacova, Iwein Gyselinck, Celine Aelbrecht, Astrid Vermaut, Hanne Beeckmans, Marie Vermant, Charlotte De Fays, Annelore Sacreas, Lucia Aversa, Michaela Orlitova, Arno Vanstapel, Ivan Josipovic, Matthieu N. Boone, John E. McDonough, Birgit Weynand, Charles Pilette, Wim Janssens, Lieven Dupont, Wim A. Wuyts, Geert M. Verleden, Dirk E. van Raemdonck, Robin Vos, Ghislaine Gayan-Ramirez, Laurens J. Ceulemans, Bart M. Vanaudenaerde
Summary: This study assessed disease progression in COVID-19 patients using in vivo CT and ex vivo microCT, and described the changes in lung volume and density as well as airway morphology. Results showed that COVID-19 lung volumes decreased and densities increased over time, mainly in the lower lobes. Ex vivo microCT revealed a 60% reduction in lung volume compared to controls, and increased visibility of airways in COVID-19 lungs, possibly due to fibrosis and increased airway diameter. Small-partition microCT further showed deformations in airway morphology and heterogeneous fibrotic organization in severely affected regions, which was confirmed by histology.
JOURNAL OF THORACIC DISEASE
(2023)
Meeting Abstract
Critical Care Medicine
C. De Fays, H. Beeckmans, P. Kerckhof, V. Geudens, A. Vermaut, I. Gyselinck, T. Goos, M. Vermant, J. Kaes, J. Van Slambrouck, Y. Mohamady, L. Willems, L. Aversa, K. Maes, C. Aelbrecht, S. Everaerts, J. E. Mcdonough, L. J. De Sadeleer, S. Gohy, J. Ambroise, W. A. Wuyts, W. L. Janssens, L. J. Ceulemans, D. E. M. Van Raemdonck, R. Vos, T. Hackett, J. C. Hogg, N. Kaminski, C. Pilette, G. Gayan-Ramirez, B. Vanaudenaerde
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Annalisa Barbarossa, Dirk Van Raemdonck, Walter Weder, Laurens J. Ceulemans
Summary: Secondary pneumothorax due to emphysema is a life-threatening condition that often requires surgery. In this case, we used LVRS to not only remove part of the lung but also close the fistula. The patient experienced improved lung function and quality of life after urgent and elective LVRS surgeries, with resolution of the air leak.
INTERDISCIPLINARY CARDIOVASCULAR AND THORACIC SURGERY
(2023)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
