Article
Biochemistry & Molecular Biology
Hanno Maassen, Leonie H. Venema, Marc G. Weiss, Tobias M. Huijink, H. Sijbrand Hofker, Anna K. Keller, Tom E. Mollnes, Marco Eijken, Soren E. Pischke, Bente Jespersen, Harry van Goor, Henri G. D. Leuvenink
Summary: Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aimed to improve the flush-out and decrease ischemic injury by adding hydrogen sulphide (H2S) to the flush medium. The addition of H2S during flush out and static cold storage resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
Review
Gastroenterology & Hepatology
Riccardo De Carlis, Paolo Muiesan, Burcin Taner
Summary: DCD grafts pose risks of complications and graft loss after liver transplantation due to prolonged functional donor warm ischaemia time. Improved outcomes have been achieved through stringent donor selection criteria and utilization of organ perfusion technologies. These technologies also enable liver function assessment and precise graft-recipient selection.
JOURNAL OF HEPATOLOGY
(2023)
Article
Immunology
Biao Lei, M. Mahdi Sleiman, Qi Cheng, Zhenxiao Tu, Peng Zhu, Martin Goddard, Paulo N. Martins, Logan Langerude, Satish Nadig, Stephen Tomlinson, Carl Atkinson
Summary: This study found that donor brain death exacerbates ischemia-reperfusion injury in vascularized composite allograft transplantation and leads to earlier rejection of the graft. Treatment of donor VCAs with CR2-Crry significantly reduced complement deposition, inflammation, and injury, providing localized protection without significant immunosuppression effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Maximilian J. Roesel, Nirmal S. Sharma, Andreas Schroeter, Tomohisa Matsunaga, Yao Xiao, Hao Zhou, Stefan G. Tullius
Summary: Transplant centers use older donors to meet the demand for lung transplantation, but this is associated with a higher rate of lung ischemia reperfusion injury. Understanding and improving the condition of older lungs is critical to optimize transplant outcomes. Ex vivo lung perfusion can recondition ischemic lungs through ex-vivo perfusing and ventilation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Medicine, Research & Experimental
Aparna C. Swaminathan, Jamie L. Todd, Scott M. Palmer
Summary: Lung transplantation has substantially increased in volume in recent decades, with advances being made in understanding and treating post-transplant complications like CLAD. Ongoing studies are exploring novel approaches to improve long-term outcomes for lung transplant recipients.
ANNUAL REVIEW OF MEDICINE, VOL 72, 2021
(2021)
Article
Biotechnology & Applied Microbiology
Valentina Veloso-Gimenez, Rosalba Escamilla, David Necunir, Rocio Corrales-Orovio, Sergio Riveros, Carlo Marino, Carolina Ehrenfeld, Christian Dani Guzman, Mauricio P. Boric, Rolando Rebolledo, Jose Tomas Egana
Summary: This study aimed to develop a perfusable photosynthetic solution with microalgae embedded to supply oxygen and support ex vivo organ preservation. The research found that the photosynthetic solution did not harm animal cells and provided sufficient oxygen to meet metabolic demands. Experimental validation showed that during organ perfusion, the photosynthetic microorganisms could reach the renal vasculature without causing tissue damage, surviving the process successfully.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ophelie Pasini-Chabot, Julia Vincent, Sylvain Le Pape, Maryne Lepoittevin, Wassim Kaaki, Jean-Baptiste Woillard, Sebastien Giraud, Nicolas Bourmeyster, Thierry Hauet, Raphael Thuillier
Summary: The study indicates that significant changes in protein expression occur in key pathways during organ preservation, with time-sensitive protein expression potentially serving as quality biomarkers for organs and targets for new strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Andrew T. Sage, Laura L. Donahoe, Alaa A. Shamandy, S. Hossein Mousavi, Bonnie T. Chao, Xuanzi Zhou, Jerome Valero, Sharaniyaa Balachandran, Aadil Ali, Tereza Martinu, George Tomlinson, Lorenzo Del Sorbo, Jonathan C. Yeung, Mingyao Liu, Marcelo Cypel, Bo Wang, Shaf Keshavjee
Summary: In this study, authors developed a machine learning model called InsighTx, which accurately predicts transplant outcomes and increases organ utilization rates. Training and test datasets showed that InsighTx model achieved a high AUROC in predicting transplant outcomes. Implementation study also demonstrated that InsighTx increased the likelihood of transplanting suitable donor lungs and decreased the likelihood of transplanting unsuitable donor lungs.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Luciana Da Silveira Cavalcante, Manuela Lopera Higuita, Juan Manuel Gonzalez-Rosa, Beatriz Marques, Samantha To, Casie A. Pendexter, Stephanie E. J. Cronin, Kaustav Gopinathan, Reinier J. de Vries, Felix Ellett, Korkut Uygun, David M. Langenau, Mehmet Toner, Shannon N. Tessier
Summary: This study demonstrates the value of zebrafish for solid organ preservation and transplantation research, especially in experimental transplantation efforts. Customized and validated methods were used to perform a large-scale screen of cryoprotectant agents, resulting in successful preservation of adult zebrafish cardiac function.
Review
Gastroenterology & Hepatology
Mauricio Flores Carvalho, Yuri L. Boteon, James V. Guarrera, Pranjal R. Modi, Laura Llado, Georg Lurje, Mureo Kasahara, Philipp Dutkowski, Andrea Schlegel
Summary: This article discusses the challenges of implementing machine perfusion of solid organs in practice, including the roles of clinical practitioners, hospitals, regulatory bodies, and industry stakeholders worldwide. It emphasizes the need for strong collaboration between these stakeholders and presents integrated roadmaps to achieve wider implementation. The importance of research development, clear regulatory pathways, and flexible reimbursement schemes is highlighted, along with potential solutions to address relevant hurdles. The article provides an overview of the current landscape of liver perfusion and underscores the role of clinical, regulatory, and financial stakeholders globally.
Article
Multidisciplinary Sciences
Laetitia Bodenes, Quang-Thang N'guyen, Raphael Le Mao, Nicolas Ferriere, Victoire Pateau, Francois Lellouche, Erwan L'Her
Summary: Heart rate variability can be used to evaluate the cardiac effects of autonomic nervous system activity, and can predict the survival of ICU patients. Machine learning can be utilized to determine a simple decision tree for predicting a composite outcome index for patients.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Joris Blondeel, Nicholas Gilbo, Veerle Heedfeld, Tine Wylin, Louis Libbrecht, Ina Jochmans, Jacques Pirenne, Hannelie Korf, Diethard Monbaliu
Summary: During normothermic machine perfusion (NMP), livers procured after warm ischemia (WI) exhibit a distinct innate immune response compared to directly procured (DP) livers. The cytokine profile in WI livers shifts towards an anti-inflammatory phenotype during NMP and whole blood (WB) reperfusion, while pro-apoptotic signaling is stronger during NMP. WB reperfusion results in a blunted cytokine release in livers, suggesting a reconditioning effect of NMP, regardless of ischemic damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
C. Corbin Frye, Amit I. Bery, Daniel Kreisel, Hrishikesh S. Kulkarni
Summary: Sterile inflammation after organ transplantation, caused by necrotic cell death and release of endogenous ligands, including DAMPs and alarmins, is a significant contributor to graft damage. Understanding the mechanistic basis of sterile inflammation is critical for identifying personalized therapies to prevent or interrupt this vicious cycle and mitigate allograft dysfunction.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anna Maria Tolomeo, Assunta Fabozzo, Ricardo Malvicini, Giada De Lazzari, Paola Bisaccia, Gianluca Gaburro, Diletta Arcidiacono, Denni Notarangelo, Federico Caicci, Fabio Zanella, Massimo Marchesan, Gustavo Yannarelli, Gianfranco Santovito, Maurizio Muraca, Gino Gerosa
Summary: In this study, it was found that using normothermic cardioplegia after warm ischemia is more effective in heart preservation, preventing oxidative stress and the subsequent inflammatory response. Specifically, it can reduce mitochondria swelling and maintain mitochondrial morphology, showing better outcomes compared to cold cardioplegia.
Article
Surgery
Marit E. van der Sande, Nuno Figueiredo, Geerard L. Beets
Summary: The study evaluated the management and outcomes of rectal cancer patients with local regrowth in a watch-and-wait program. Most patients with regrowth were able to undergo surgical treatment with curative intent, and uncontrolled pelvic disease was very rare.
Article
Immunology
Raffaele Brustia, Antoine Monsel, Stefano Skurzak, Eduardo Schiffer, Francois Martin Carrier, Damiano Patrono, Abdourahamane Kaba, Olivier Detry, Luiz Malbouisson, Wellington Andraus, Franck Vandenbroucke-Menu, Gianni Biancofiore, Toshimi Kaido, Philippe Compagnon, Shinji Uemoto, Gonzalo Rodriguez Laiz, Marieke De Boer, Susan Orloff, Paola Melgar, Carlijn Buis, Miriam Zeillemaker-Hoekstra, Helen Usher, Koen Reyntjens, Emily Baird, Nicolas Demartines, Stephen Wigmore, Olivier Scatton
Summary: This study presents the specific ERAS Society recommendations for liver transplantation (LT) based on the best available evidence and expert consensus. Screening and treatment for malnutrition in patients are recommended. Prophylactic nasogastric intubation and abdominal drainage may be omitted, and early extubation should be considered. Early oral intake, mobilization, and multimodal-balanced analgesia are also recommended.
Letter
Surgery
Olivier Detry, Frederik Berrevoet, Filip Muysoms
UPDATES IN SURGERY
(2022)
Article
Medical Laboratory Technology
Pierre Delanaye, Francois Gaillard, Jessica van der Weijden, Geir Mjoen, Ingela Ferhman-Ekholm, Laurence Dubourg, Natalie Ebert, Elke Schaeffner, Torbjorn Akerfeldt, Karolien Goffin, Lionel Couzi, Cyril Garrouste, Lionel Rostaing, Marie Courbebaisse, Christophe Legendre, Maryvonne Hourmant, Nassim Kamar, Etienne Cavalier, Laurent Weekers, Antoine Bouquegneau, Martin H. de Borst, Christophe Mariat, Hans Pottel, Marco van Londen
Summary: This study calculated GFR percentiles from donors under 65 years old and successfully extrapolated and validated them in donors and healthy subjects over 65 years old, showing that younger donors have higher mGFR than elderly donors. This model may aid in decision-making for elderly donors.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Gastroenterology & Hepatology
Andrea Schlegel, Marjolein van Reeven, Kristopher Croome, Alessandro Parente, Annalisa Dolcet, Jeannette Widmer, Nicolas Meurisse, Riccardo De Carlis, Amelia Hessheimer, Ina Jochmans, Matteo Mueller, Otto B. van Leeuwen, Amit Nair, Koji Tomiyama, Ahmed Sherif, Mohamed Elsharif, Philipp Kron, Danny van der Helm, Daniel Borja-Cacho, Humberto Bohorquez, Desislava Germanova, Daniele Dondossola, Tiziana Olivieri, Stefania Camagni, Andre Gorgen, Damiano Patrono, Matteo Cescon, Sarah Croome, Rebecca Panconesi, Mauricio Flores Carvalho, Matteo Ravaioli, Juan Carlos Caicedo, George Loss, Valerio Lucidi, Gonzalo Sapisochin, Renato Romagnoli, Wayel Jassem, Michele Colledan, Luciano De Carlis, Giorgio Rossi, Fabrizio Di Benedetto, Charles M. Miller, Bart van Hoek, Magdy Attia, Peter Lodge, Roberto Hernandez-Alejandro, Olivier Detry, Cristiano Quintini, Gabriel C. Oniscu, Constantino Fondevila, Massimo Malago, Jacques Pirenne, Jan N. M. IJzermans, Robert J. Porte, Philipp Dutkowski, C. Burcin Taner, Nigel Heaton, Pierre-Alain Clavien, Wojciech G. Polak, Paolo Muiesan
Summary: The study established the best possible outcomes and reference values for liver transplantation using grafts donated after circulatory death (DCD) through benchmarking. The benchmark cases showed excellent 1-year retransplant and mortality rates, but had high rates of major complications. The use of organ perfusion technology reduced complications and improved outcomes in high-risk patients.
JOURNAL OF HEPATOLOGY
(2022)
Editorial Material
Urology & Nephrology
Pauline Erpicum, Stephanie Grosch, Catherine Bonvoisin, Antoine Bouquegneau, Laurent Weekers, Luc Radermacher, Francois Jouret, Marie Lejeune, Olivier Hougrand, Christophe Bovy
KIDNEY INTERNATIONAL
(2022)
Article
Gastroenterology & Hepatology
Morgan Vandermeulen, Maleyko Mohamed-Wais, Pauline Erpicum, Marie-Helene Delbouille, Chantal Lechanteur, Alexandra Briquet, Gianni Maggipinto, Francois Jouret, Yves Beguin, Olivier Detry
Summary: The study confirmed the safety of a single MSC infusion after LT with no significant increase in infection or cancer risk, but a higher incidence of liver DSAs in the MSC group. Further evaluation of anti-HLA antibodies against MSC donors is warranted.
LIVER TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Barbara Chiavarina, Roberto Ronca, Yukihiro Otaka, Roger Bryan Sutton, Sara Rezzola, Takehiko Yokobori, Paola Chiodelli, Regis Souche, Didier Pourquier, Antonio Maraver, Gavino Faa, Lakhdar Khellaf, Evgenia Turtoi, Tetsunari Oyama, Stephanie Gofflot, Akeila Bellahcene, Olivier Detry, Philippe Delvenne, Vincent Castronovo, Masahiko Nishiyama, Andrei Turtoi
Summary: Cancer-associated fibroblasts (CAF) play important roles in tumor microenvironment and tumorigenesis. In hepatocellular carcinoma (HCC), there are three major CAF populations, one of which expresses prolargin protein. Prolargin acts as an angiogenesis modulator and tumor suppressor in HCC, inhibiting the activity of pro-angiogenic proteins. Stabilizing prolargin levels in the CAF_Port subpopulation may reverse their tumor-antagonizing properties.
Article
Immunology
Beatriz Dominguez-Gil, Kerstin Moench, Christopher Watson, M. Trinidad Serrano, Taizo Hibi, Jose M. Asencio, Marieke Van Rosmalen, Olivier Detry, Julie Heimbach, Francois Durand
Summary: Liver transplantation carries the risk of donor-transmitted cancers (DTCs), with most cases being unidentified prior to transplantation. The International Liver Transplantation Society has provided guidance on minimizing DTC occurrence and the classification of malignancies based on transmission risk. Immediate notification and multidisciplinary approaches are recommended for managing DTC events.
Article
Medicine, General & Internal
Louis Firket, Antoine Bouquegneau, Laurence Seidel, Catherine Bonvoisin, Stephanie Grosch, Marie-Pierre Hayette, Francois Jouret, Laurent Weekers
Summary: This study provides insights into the occurrence and humoral immune response of COVID-19 in kidney transplant recipients. The results show a significant incidence of COVID-19 in this population, but a considerable proportion of patients develop persistent humoral immunity post-COVID-19.
ACTA CLINICA BELGICA
(2023)
Article
Surgery
Vincent Tchana-Sato, Gregory Hans, Janne Brouckaert, Olivier Detry, Johan Van Cleemput, Steffen Rex, Oceane Jaquet, Erwin De Troy, Mai-Linh Nguyen Trung, Arnaud Ancion, Raf Van den Eynde, Isabelle Lievens, Marc Gilbert Lagny, Marie-Helene Delbouille, Jean Olivier Defraigne, Didier Ledoux, Filip Rega
Summary: This study reports the first case of heart donation after euthanasia, demonstrating the feasibility of using specific repair measures and preservation methods to successfully perform a heart transplant.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Editorial Material
Surgery
Maud Neuberg, Olivier Detry
ACTA CHIRURGICA BELGICA
(2023)
Article
Surgery
Clara Bihain, Jean Delwaide, Paul Meunier, Laurent Gerard, Alexandre Jadoul, Olivier Detry
Summary: The case report describes a large hepatocellular carcinoma (HCC) found in a non-cirrhotic liver. The patient underwent selective internal radiation therapy and surgery, showing no signs of HCC recurrence after 30 months. The discussion highlights the better prognosis of HCC in non-cirrhotic liver and the challenges of late diagnosis and inadequate screening.
ACTA CHIRURGICA BELGICA
(2023)
Article
Medicine, General & Internal
Petra Hruba, Jiri Klema, Anh Vu Le, Eva Girmanova, Petra Mrazova, Annick Massart, Dita Maixnerova, Ludek Voska, Gian Benedetto Piredda, Luigi Biancone, Ana Ramirez Puga, Nurhan Seyahi, Mehmet Sukru Sever, Laurent Weekers, Anja Muhfeld, Klemens Budde, Bruno Watschinger, Marius Miglinas, Ivan Zahradka, Marc Abramowicz, Daniel Abramowicz, Ondrej Viklicky
Summary: This study used RNA sequencing to identify transcripts that can distinguish operational tolerance from chronic active antibody-mediated rejection, leading to the construction of models for predicting kidney allograft loss.
Article
Urology & Nephrology
Gregoire Masson, Tommaso Viva, Justine Huart, Laurent Weekers, Catherine Bonvoisin, Antoine Bouquegneau, Sylvie Maweja, Etienne Hamoir, Laurence Seidel, Hans Pottel, Patrizio Lancellotti, Francois Jouret
Summary: Surgical closure of mature AVF in kidney transplant recipients leads to increased diastolic blood pressure, decreased serum NT-proBNP levels, reduction in left ventricular and atrial dimensions, and stable eGFR slope.
Meeting Abstract
Transplantation
Antoine Bouquegneau, Olivier Malaise, Laurence Seidel, Catherine Bonvoisin, Laurent Weekers, Francois Jouret, Etienne Cavalier, Pierre Delanaye
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
