Article
Biochemistry & Molecular Biology
Hua Zhang, Vamsi Krishna Polavarapu, Pengwei Xing, Miao Zhao, Lucy Mathot, Linxuan Zhao, Gabriela Rosen, Fredrik J. Swartling, Tobias Sjoblom, Xingqi Chen
Summary: Archived FFPE samples are widely used for cancer research, and we developed a highly sensitive method (FFPE-ATAC) to decode chromatin accessibility in FFPE tissues. This method generates high-quality chromatin accessibility profiles and allows analysis of chromatin profiles in regions of interest from a single FFPE tissue section.
Article
Biology
Masachika Ikegami, Shinji Kohsaka, Takeshi Hirose, Toshihide Ueno, Satoshi Inoue, Naoki Kanomata, Hideko Yamauchi, Taisuke Mori, Shigeki Sekine, Yoshihiro Inamoto, Yasushi Yatabe, Hiroshi Kobayashi, Sakae Tanaka, Hiroyuki Mano
Summary: The researchers developed MicroSEC, a filtering pipeline to remove sequencing artifacts from archival formalin-fixed and paraffin-embedded samples. The sensitivity and specificity of MicroSEC were confirmed to be 97% and 96% respectively using amplicon-based sequencing with 97 mutations. This tool may improve the reliability of clinical sequencing and advance cancer research using formalin-fixed, paraffin-embedded samples, which are of great interest for genomic analysis.
COMMUNICATIONS BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Norifusa Iwahashi, Hironobu Umakoshi, Masatoshi Ogata, Tazuru Fukumoto, Hiroki Kaneko, Eriko Terada, Shunsuke Katsuhara, Naohiro Uchida, Katsuhiko Sasaki, Maki Yokomoto-Umakoshi, Yayoi Matsuda, Ryuichi Sakamoto, Yoshihiro Ogawa
Summary: This study evaluated the utility of transcriptome data from formalin-fixed paraffin-embedded (FFPE) samples in adrenocortical tumor research. The results demonstrated that FFPE samples can serve as a reliable alternative to fresh frozen samples for whole transcriptome profiling of adrenocortical tumors, enabling distinction of tumor types and detection of marker genes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Linxuan Zhao, Pengwei Xing, Vamsi Krishna Polavarapu, Miao Zhao, Blanca Valero-Martinez, Yonglong Dang, Nagaprathyusha Maturi, Lucy Mathot, Ines Neves, Irem Yildirim, Fredrik Johansson Swartling, Tobias Sjoblom, Lene Uhrbom, Xingqi Chen
Summary: The study introduces a new method (FACT-seq) for efficient analysis of histone modifications in archived FFPE tissues, significantly improving resolution. High-quality chromatin profiles can be generated using a small number of FFPE nuclei, offering insights into epigenetic regulation in cancer and human disease.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Allison K. Miller, Cara L. Brosnahan, Anjali Pande, Cindy F. Baker, Jemma L. Geoghegan, Jane Kitson, Neil J. Gemmell, Edwina J. Dowle
Summary: Infectious diseases have a significant impact on various organisms, and understanding the interactions between hosts and pathogens is crucial for their conservation and management. The use of genomic approaches has made it easier to obtain this knowledge quickly, however, many species still face challenges in accessing appropriate samples and data. Archival materials, such as formalin-fixed paraffin-embedded tissue samples, may provide a valuable resource for studying pathogen emergence and host responses over long periods of time.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Genetics & Heredity
Shen Yin, Xiaowei Zhan, Bo Yao, Guanghua Xiao, Xinlei Wang, Yang Xie
Summary: RNA-seq provides comprehensive quantification of transcriptomic activities in biological samples. This study introduces a new normalization method, SMIXnorm, for FFPE RNA-seq data, which simplifies the computational process, improves efficiency, and maintains superior performance compared to existing methods. Furthermore, a web-based tool, RSeqNorm, is developed to compute normalized RNA-seq data for both FFPE and FF samples, offering users a simple workflow for downstream analysis.
FRONTIERS IN GENETICS
(2021)
Review
Genetics & Heredity
Gerardo Cazzato, Concetta Caporusso, Francesca Arezzo, Antonietta Cimmino, Anna Colagrande, Vera Loizzi, Gennaro Cormio, Teresa Lettini, Eugenio Maiorano, Vincenza Sara Scarcella, Paola Tarantino, Maricla Marrone, Alessandra Stellacci, Paola Parente, Paolo Romita, Aurora De Marco, Vincenzo Venerito, Caterina Foti, Giuseppe Ingravallo, Roberta Rossi, Leonardo Resta
Summary: Advancements in pathology have shifted towards molecular and genetic studies, allowing for the implementation of targeted therapies; however, issues may arise in NGS due to damage to genetic material in fixed samples.
Article
Biochemistry & Molecular Biology
Omar Garcia-Perez, Leticia Melgar-Vilaplana, Elizabeth Cordoba-Lanus, Ricardo Fernandez-de-Misa
Summary: This study quantified the expression of reference genes in cutaneous malignant melanoma and cutaneous squamous cell carcinoma samples, determining that HPRT1 and ACTB genes are the most stable choice in MM samples, while HPRT1 and TFRC genes are the best option in CSCC samples for normalization in gene expression studies.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Analytical
Kenneth Weke, Sachin Kote, Jakub Faktor, Sofian Al Shboul, Naomi Uwugiaren, Paul M. Brennan, David R. Goodlett, Ted R. Hupp, Irena Dapic
Summary: Developments in quantitative proteomics and DIA methodology have enabled protein quantification in biological samples, including formalin-fixed paraffin-embedded (FFPE) tissues. In this study, a simple and precise DIA-MS workflow was established to detect and quantify immune- and glioblastoma (GBM)-relevant proteins in FFPE patient materials. The results showed successful detection and quantification of more than 1700 proteins, including GBM-relevant proteins and immune-related proteins. This strategy has great potential for routine protein quantification in FFPE tissue samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Liang Zong, Yabing Zhu, Yuan Jiang, Ying Xia, Qun Liu, Sanjie Jiang
Summary: RNA-Seq analysis of FFPE samples is a highly effective approach but degradation of RNAs limits its application. A new RNA-Seq method has been developed to overcome this limitation. This study compares and evaluates three commercially available exome capture kits, providing valuable guidance for obtaining high-quality data from FFPE samples.
Review
Biochemistry & Molecular Biology
Ekenedirichukwu N. Obi, Daniel A. Tellock, Gabriel J. Thomas, Timothy D. Veenstra
Summary: The recent developments in mass spectrometry (MS) have provided new opportunities in biomarker discovery and diagnostics, particularly in analyzing clinical samples at a deeper level. While most developments have focused on blood, there is potential in utilizing archived formalin-fixed paraffin-embedded (FFPE) tissues, which were previously considered difficult to analyze using proteomics techniques. Researchers have developed methods to extract proteins from FFPE tissues, allowing for their analysis using state-of-the-art technologies like MS and protein arrays. This review discusses the history of these developments and their current applications in identifying biomarkers and diagnosing diseases such as cancer.
Article
Chemistry, Analytical
Yunxia Guo, Junjie Ma, Zhengyue Li, Kaitong Dang, Qinyu Ge, Yan Huang, Guang-zhong Wang, Xiangwei Zhao
Summary: This study investigates the effects of fixation and storage time on the nuclear transcriptome signatures of the brain. The fixation time has a greater impact on the transcriptomic profiles than the retention time of FFPE samples. The findings will guide the preparation of FFPE samples for single-nucleus RNA-seq, especially in transcriptomic studies of brain diseases.
ANALYTICA CHIMICA ACTA
(2023)
Article
Medicine, Research & Experimental
Philippe Decruyenaere, Kimberly Verniers, Franco Poma-Soto, Jo Van Dorpe, Fritz Offner, Jo Vandesompele
Summary: This study compared the impact of three RNA extraction methods on yield, quality, and sequencing-based gene expression results in FFPE samples. The results showed that methods B and C outperformed method A, resulting in higher fractions of uniquely mapped reads, an increased number of detectable genes, a lower fraction of duplicated reads, and better representation of the B-cell receptor repertoire. Caution should be applied when comparing and interpreting results obtained using different methods.
LABORATORY INVESTIGATION
(2023)
Article
Chemistry, Analytical
Hao Chen, Yuefei Zhang, Haichao Zhou, Weiran Chen, Jiayi Peng, Yang Feng, Linyuan Fan, Jun Li, Jin Zi, Yan Ren, Qidan Li, Siqi Liu
Summary: Spatial proteomics plays an important role in the era of single-cell biology. In this study, a new routine workflow was developed for proteomic analysis of micro-FFPE tissues, allowing the construction of a spatial proteomic atlas of mouse brain, which exhibited typical characteristics of spatial-dependent protein abundance and functional enrichment.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Katarina Vitosevic, Danijela Todorovic, Zivana Slovic, Tatjana Varljen, Ivana Radakovic, Dusan Radojevic, Vanja Canovic, Milos Todorovic
Summary: This study compared the quality of DNA isolated from fixed tissues and tissues embedded in paraffin blocks after fixation, finding that the fixation time and embedding time have an impact on DNA yield and integrity.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Eleni Kanouta, Jacob Graversen Johansen, Gustavo Kertzscher, Mateusz Krzysztof Sitarz, Brita Singers Sorensen, Per Rugaard Poulsen
Summary: A fast scintillator detector system was developed to measure the spot and transition duration in FLASH treatments. The detector system was tested in both phantom and in vivo experiments, showing good agreement with log files and demonstrating its feasibility for preclinical studies.
Article
Genetics & Heredity
Pernille A. Gregersen, Mikkel Funding, Jan Alsner, Maja H. Olsen, Jens Overgaard, Steen F. Urbak, Sandra E. Staffieri, Stina Lou
Summary: Survivors of heritable retinoblastoma face complex and difficult reproductive decision-making, with a strong desire to avoid passing on the disease to their children. They consider various medical, practical, emotional, and moral issues in their family planning process, and express feelings of guilt. Healthcare professionals should provide necessary information and tools to support their unique decision-making process.
JOURNAL OF GENETIC COUNSELING
(2023)
Article
Oncology
Birgitte Sandfeld-Paulsen, Ninna Aggerholm-Pedersen, Anne Winther-Larsen
Summary: This study evaluated the prognostic significance of platelet count in lung cancer patients. The results showed that low and high platelet count were significantly correlated with inferior overall survival in non-small-cell lung cancer (NSCLC) patients, while low platelet count was significantly associated with inferior overall survival in small-cell lung cancer (SCLC) patients.
CLINICAL LUNG CANCER
(2023)
Article
Oncology
Mette Abildgaard Pedersen, Thomas Baad-Hansen, Lars C. Gormsen, Steen Baerentzen, Birgitte Sandfeld-Paulsen, Ninna Aggerholm-Pedersen, Mikkel Holm Vendelbo
Summary: This study aimed to evaluate the prognostic accuracy of combining PET parameters and circulating biomarkers in sarcoma patients. The results showed that adding PET parameters to existing prognostic models improved the prognostic value in all models. A new prognostic model, SBSpib, which includes albumin, lymphocytes, and metabolic tumor volume (MTV) as PET parameter, was developed. The study suggests that combining PET parameters and circulating biomarkers can enhance prognostic evaluation, but further validation is needed for SBSpib.
Article
Oncology
Anneli Dowler Nygaard, Ninna Aggerholm Pedersen, Gitte Aaroe Dam, Marianne Marquard Knap, Elizaveta Mitkina Tabaksblat
Summary: This study suggests that SBRT may be a feasible and effective treatment for neuroendocrine neoplasms in selected cases. SBRT provides long-term local stability and may be useful in treating patients with localized disease not fit for surgery.
Article
Public, Environmental & Occupational Health
Anne-Mette Iversen, Marco Bo Hansen, Jan Alsner, Brian Kristensen, Svend Ellermann-Eriksen
Summary: Nudging with sensor lights can improve and sustain the hand hygiene compliance of physicians and nurses, reducing hospital-acquired infections.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Oncology
Judit Kisistok, Ditte Sigaard Christensen, Mads Heilskov Rasmussen, Lone Duval, Ninna Aggerholm-Pedersen, Adam Andrzej Luczak, Boe Sandahl Sorensen, Martin Roelsgaard Jakobsen, Trine Heide Oellegaard, Nicolai Juul Birkbak
Summary: Immunotherapy has dramatically improved the treatment outcomes for metastatic melanoma patients, but it also brings immune-related adverse events. Identifying nonresponding patients early is crucial, and this study explores the use of circulating tumor DNA (ctDNA) analysis as a minimally invasive tool. In a cohort of 24 melanoma patients, the presence of ctDNA was associated with poor prognosis, suggesting its potential to be used in clinical practice.
Article
Oncology
Marie Louise Holm Milo, Ditte Slot Moller, Tine Bisballe Nyeng, Lone Hoffmann, Henrik Dahl Nissen, Ingelise Jensen, Ebbe Laugaard Lorenzen, Lise Bech Jellesmark Thorsen, Kirsten Melgaard Nielsen, Soren Paaske Johnsen, Jan Brink Valentin, Jan Alsner, Birgitte Vrou Offersen
Summary: This study aimed to report the radiation therapy (RT) doses to the heart and cardiac substructures in breast cancer patients treated with CT-based RT and investigate the potential dose-response relationship between RT dose and coronary artery disease (CAD). The results showed that CAD tended to occur in the part of the heart with the highest left-minus-right dose difference, but no significant risk of CAD was observed at a median follow-up of 7 years.
RADIOTHERAPY AND ONCOLOGY
(2023)
Article
Oncology
Ninna Aggerholm-Pedersen, Henriette Nymark Friis, Thomas Baad-Hansen, Holger Jon Moller, Birgitte Sandfeld-Paulsen
Summary: About 40 percent of patients with primary sarcoma experience relapse after treatment, leading to a low survival rate. Previous studies have failed to improve survival through adjuvant chemotherapy. This study provides a prognostic profile for sarcoma patients, identifying those who may not require additional treatment and those who would benefit from adjuvant treatment. The study also highlights the importance of the innate immune system in the prognosis of sarcoma patients.
Review
Health Care Sciences & Services
Anne Iren Riskjell, Vivi-Nelli Makinen, Birgitte Sandfeld-Paulsen, Ninna Aggerholm-Pedersen
Summary: This study provides an overview of the clinical experiences with targeted treatments in patients with soft-tissue sarcoma (STS). MDM2 inhibitors were the most commonly studied drug, with promising results. However, more evidence is needed to determine the efficacy of other targeted agents for specific genetic alterations in STS.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Oncology
Cathrine Bang Overgaard, Fardous Reaz, Mateusz Sitarz, Per Poulsen, Jens Overgaard, Niels Bassler, Cai Grau, Brita Singers Sorensen
Summary: This study introduced an experimental radiobiological setup for in vivo irradiation of a mouse leg target along a proton beam path. The setup was used to investigate normal tissue and tumor models with varying linear energy transfer. The results showed that acute skin damage was reversible, while radiation-induced late damage was irreversible.
Article
Oncology
Ninna Aggerholm-Pedersen, Thomas Baad-Hansen, Holger Jon Moller, Birgitte Sandfeld-Paulsen
Summary: This study investigated the prognostic value of high-sensitivity C-reactive protein (hs-CRP) in patients with sarcoma and found that elevated levels of hs-CRP were associated with increased overall mortality and decreased progression-free survival.
Article
Oncology
Sofie Tilbaek, Ludvig Paul Muren, Anne Vestergaard, Liliana Stolarczyk, Heidi S. Ronde, Tanja S. Johansen, Jimmi Sondergaard, Morten HOyer, Jan Alsner, Lise Norgaard Bentzen, Stine Elleberg Petersen
Summary: The Danish Prostate Cancer Group is conducting a randomized trial comparing photons and protons for the treatment of high-risk prostate cancer. The aim of this study was to establish a feasible and robust strategy for conventionally fractionated proton therapy of the prostate and pelvic lymph nodes in preparation for this trial. Proton treatments are guided by imaging systems and optimized to minimize range uncertainties and reduce gastrointestinal toxicity.
CLINICAL AND TRANSLATIONAL RADIATION ONCOLOGY
(2023)
Article
Oncology
Elnaz Naderi, Miguel E. Aguado-Barrera, Line M. H. Schack, Leila Dorling, Tim Rattay, Laura Fachal, Holly Summersgill, Laura Martinez-Calvo, Ceilidh Welsh, Tom Dudding, Yasmin Odding, Ana Varela-Pazos, Rajesh Jena, David J. Thomson, Roel J. H. M. Steenbakkers, Joe Dennis, Ramon Lobato-Busto, Jan Alsner, Andy Ness, Chris Nutting, Antonio Gomez-Caamano, Jesper G. Eriksen, Steve J. Thomas, Amy M. Bates, Adam J. Webb, Ananya Choudhury, Barry S. Rosenstein, Begona Taboada-Valladares, Carsten Herskind, David Azria, David P. Dearnaley, Dirk de Ruysscher, Elena Sperk, Emma Hall, Hilary Stobart, Jenny Chang-Claude, Kim De Ruyck, Liv Veldeman, Manuel Altabas, Maria Carmen De Santis, Marie-Pierre Farcy-Jacquet, Marlon R. Veldwijk, Matthew R. Sydes, Matthew Parliament, Nawaid Usmani, Neil G. Burnet, Petra Seibold, R. Paul Symonds, Rebecca M. Elliott, Renee Bultijnck, Sara Gutierrez-Enriquez, Meritxell Molla, Sarah L. Gulliford, Sheryl Green, Tiziana Rancati, Victoria Reyes, Ana Carballo, Paula Peleteiro, Paloma Sosa-Fajardo, Chris Parker, Valerie Fonteyne, Kerstie Johnson, Maarten Lambrecht, Ben Vanneste, Riccardo Valdagni, Alexandra Giraldo, Monica Ramos, Brenda Diergaarde, Geoffrey Liu, Suzanne M. Leal, Melvin L. K. Chua, Miranda Pring, Jens Overgaard, Luis M. Cascallar-Caneda, Frederic Duprez, Christopher J. Talbot, Gillian C. Barnett, Alison M. Dunning, Ana Vega, Christian Nicolaj Andreassen, Johannes A. Langendijk, Catharine M. L. West, Behrooz Z. Alizadeh, Sarah L. Kerns
Summary: This study identified shared genetic susceptibility for acute radiation-induced toxicity across different cancer types, with a shared SNV-based heritability estimated at around 10%. Higher heritability rates were found for prostate, head and neck, and breast cancers. Additionally, several potentially associated SNVs and gene sets related to acute toxicity were discovered.
JNCI CANCER SPECTRUM
(2023)
Meeting Abstract
Oncology
T. Tramm, P. S. Nielsen, J. B. Georgsen, J. Overgaard, J. Alsner
RADIOTHERAPY AND ONCOLOGY
(2022)
Article
Oncology
Xiaofan Pu, Chaolei Zhang, Guoping Ding, Hongpeng Gu, Yang Lv, Tao Shen, Tianshu Pang, Liping Cao, Shengnan Jia
Summary: This study demonstrated the potential utility of the sEV-miRNA d-signature in the diagnosis of PDAC via machine learning methods. A novel sEV biomarker, miR-664a-3p, was identified for the diagnosis of PDAC. It can also potentially promote angiogenesis and metastasis, provide insight into PDAC pathogenesis, and reveal novel regulators of this disease.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Jiaping Wang, Zhijuan Xu, Yanli Lai, Yanli Zhang, Ping Zhang, Qitian Mu, Shujun Yang, Yongcheng Sun, Lixia Sheng, Guifang Ouyang
Summary: This study demonstrates the significance of PD-1 in EBV-infected lymphoma cells. Silencing PD-1 enhances the tumor targeting effect of EBV-specific killer T cells on B lymphocytes and attenuates the immune escape effect.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Qiliang Peng, Jialong Tao, Yingjie Xu, Yi Shen, Yong Wang, Yang Jiao, Yiheng Mao, Yaqun Zhu, Yulong Liu, Ye Tian
Summary: This study investigates the potential role of lipid metabolism-associated genes (LMAGs) in neoadjuvant chemoradiotherapy (nCRT) and immunotherapy for rectal cancer. The results suggest that the SREBF2 gene is a highly predictive factor for nCRT in rectal cancer and is associated with favorable prognosis. SREBF2 is also closely associated with immune cell infiltration and immunotherapy-related genes.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Shiquan Li, Nan Zhang, Yongping Yang, Tongjun Liu
Summary: This study investigated the potential molecular mechanism of SPDEF in immune evasion of colorectal cancer (CRC) and found that it suppresses immune evasion by activating CCL28 through the modulation of M2 polarization of macrophages. These findings provide a new research direction and potential therapeutic target for immunotherapy in CRC.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Manas Sehgal, Soundharya Ramu, Joel Markus Vaz, Yogheshwer Raja Ganapathy, Srinath Muralidharan, Sankalpa Venkatraghavan, Mohit Kumar Jolly
Summary: This study investigates the relationship between gene expression patterns and phenotypic plasticity and heterogeneity in colorectal cancer (CRC). The results demonstrate the interconnectedness between different Consensus Molecular Subtypes (CMS) of CRC and specific phenotypes such as epithelial and mesenchymal characteristics. Additionally, the study reveals correlations between metabolic pathways and phenotypic scores, as well as between PD-L1 activity and mesenchymal phenotype. Single-cell RNA sequencing analysis further confirms the heterogeneity of different CMS subtypes. These findings have important implications for understanding CRC heterogeneity and developing targeted therapies.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Yutong Zou, Siyao Guo, Yan Liao, Weidong Chen, Ziyun Chen, Junkai Chen, Lili Wen, Xianbiao Xie
Summary: This study found that ceramide metabolism is associated with the progression and clinical outcome of osteosarcoma by analyzing data from osteosarcoma patients. The gene ST3GAL1 plays an important role in osteosarcoma, regulating the tumor immune microenvironment and affecting T cell function. It may become a new target for the treatment of osteosarcoma.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Chuanhui Chen, Mengzhi Wan, Xiong Peng, Qing Zhang, Yu Liu
Summary: This study examines the function and mechanism of the ceRNA network centered around GPR37 in LUAD. The findings show that high expression of GPR37 in LUAD tissue samples is associated with poor prognosis, and it may regulate the expression of downstream target genes by competitively binding to lncRNA DLEU1 and miR-4458.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Junping Li, Hong Hu, Jinping He, Yuling Hu, Manting Liu, Bihui Cao, Dongni Chen, Xiaodie Ye, Jian Zhang, Zhiru Zhang, Wen Long, Hui Lian, Deji Chen, Likun Chen, Lili Yang, Zhenfeng Zhang
Summary: Sequential administration of CDC7 inhibitor XL413 after carboplatin enhances the chemotherapeutic effect of carboplatin on ovarian cancer cells, possibly by inhibiting homologous recombination repair activity and increasing the accumulation of chemotherapy-induced DNA damage.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Madison Catalanotto, Joel Markus Vaz, Camille Abshire, Reneau Youngblood, Min Chu, Herbert Levine, Mohit Kumar Jolly, Ana -Maria Dragoi
Summary: The study demonstrates that loss of FLASH in cancer cells leads to a hybrid E/M phenotype with high epithelial scores, suggesting FLASH acts as a repressor of the epithelial phenotype. Additionally, FLASH expression is inversely correlated with the epithelial score and subsets of mesenchymal markers are distinctly up-regulated in FLASH, NPAT, or SLBP-depleted cells.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Xiaorui Wang, Na Li, Minying Zheng, Yongjun Yu, Shiwu Zhang
Summary: Adipocytes are derived from pluripotent mesenchymal stem cells and histone modifications play a key role in their differentiation. Recent studies have shown that cancer stem cells can differentiate into adipocytes, reducing the malignancy of cancer cells.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Hana Q. Sadida, Alanoud Abdulla, Sara Al Marzooqi, Sheema Hashem, Muzafar A. Macha, Ammira S. Al-Shabeeb Akil, Ajaz A. Bhat
Summary: Cancer heterogeneity and drug resistance are major obstacles to effective cancer treatment, and epigenetic modifications play a pivotal role in these processes. This review explores essential epigenetic modifications, including DNA methylation, histone modifications, and chromatin remodeling, and discusses their complex contributions to cancer biology. However, the interplay of epigenetic and genetic changes in cancer cells presents unique challenges that must be addressed to fully exploit the potential of epigenetic modifications.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Pedro De Marchi, Leticia Ferro Leal, Luciane Sussuchi da Silva, Rodrigo de Oliveira Cavagna, Flavio Augusto Ferreira da Silva, Vinicius Duval da Silva, Eduardo C. A. da Silva, Augusto O. Saito, Vladmir C. Cordeiro de Lima, Rui Manuel Reis
Summary: The TIS and IFN-gamma signatures are predictive biomarkers for identifying NSCLC patients who could potentially benefit from immune checkpoint inhibitor therapies.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Giovanni Marchi, Anna Rajavuori, Mai T. N. Nguyen, Kaisa Huhtinen, Sinikka Oksa, Sakari Hietanen, Sampsa Hautaniemi, Johanna Hynninen, Jaana Oikkonen
Summary: The study shows that ctDNA can adequately represent high-grade serous ovarian carcinoma (HGSC), and the mutations observed at relapse suggest personalized therapy options.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Yuncang Yuan, Jiawei Fan, Dandan Liang, Shijie Wang, Xu Luo, Yongjie Zhu, Nan Liu, Tingxiu Xiang, Xudong Zhao
Summary: This study demonstrates that csGRP78-directed CAR-T cells can selectively kill pancreatic cancer cells, and the combination with chemotherapy enhances cytotoxicity.
TRANSLATIONAL ONCOLOGY
(2024)
Article
Oncology
Niyati Piplani, Tanusri Roy, Neha Saxena, Shamik Sen
Summary: The glycocalyx, a protective barrier surrounding cells, has been found to play a role in cancer cell proliferation, survival, and metastasis. However, its function in maintaining DNA/nuclear integrity during migration through dense matrices has not been explored. This study shows that the bulkiness of the glycocalyx is inversely associated with nuclear stresses, and highlights its mechanical role in shielding migration-associated stresses.
TRANSLATIONAL ONCOLOGY
(2024)
