Article
Biochemistry & Molecular Biology
Irena Krueger, Friedrich Reusswig, Kim Juergen Krott, Celina Fabienne Lersch, Martina Spelleken, Margitta Elvers
Summary: The study demonstrates that the mT/mG Cre reporter mouse is a suitable model for real-time visualization of platelets, analysis of cell morphology, and identification of non-recombined platelets. However, a small proportion of leukocytes exhibit mG fluorescence in PF4-Cre positive mice. Therefore, careful evaluation of platelet analysis beyond hemostasis and thrombosis should be conducted when increasing recombination of immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yuto Kuwasaki, Kazushi Suzuki, Gaigai Yu, Shota Yamamoto, Takahiro Otabe, Yuki Kakihara, Michiru Nishiwaki, Keita Miyake, Keiji Fushimi, Ramsey Bekdash, Yoshihiro Shimizu, Rei Narikawa, Takahiro Nakajima, Masayuki Yazawa, Moritoshi Sato
Summary: This study developed a red light-activatable photoswitch called MagRed, which incorporates a red light-absorbing bacterial phytochrome, a mammalian endogenous chromophore, biliverdin, and a photo-state-specific binder. Using MagRed, a red light-activatable Cre recombinase and red light-inducible transcriptional regulators based on CRISPR-Cas9 were developed, enabling optical control of genetic manipulations in deep mammalian tissues.
NATURE BIOTECHNOLOGY
(2022)
Article
Immunology
Thao Vo, Yogesh Saini
Summary: The Cre-LoxP system is commonly used for recombination of floxed genes, but current macrophage-specific Cre recombinase mouse strains have limitations. This study tested the Mafb-Cre strain, which labels macrophages in various organs. The results show that Mafb-Cre only targets around 40% of alveolar macrophages, regardless of age. Mafb+ macrophages exhibit higher expression of surface markers. Bone marrow-derived macrophages are highly amenable to Cre-LoxP recombination, and Th2 stimulation and ozone exposure promote Mafb-Cre-mediated recombination in alveolar macrophages.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jin Young Jeong, Ki Ryang Na, Jin Ah Shin, Kwang-Sun Suh, Jwa-Jin Kim, Kang Wook Lee, Dae Eun Choi
Summary: This study revealed that collecting duct-specific mitochondrial injury may exacerbate inflammation and fibrosis in chronic kidney disease, and could worsen renal injury induced by unilateral ureteral obstruction (UUO). Mitochondrial damage associated oxidative stress may play a role in worsening UUO-induced renal injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Ruochen Liu, Qin Long, Xiuping Zou, You Wang, Yan Pei
Summary: The low DNA recombination efficiency of site-specific recombinase systems in plants leads to gene silencing rather than gene deletion, mainly due to hypermethylation of the floxed region which prevents gene deletion and silences gene expression.
Editorial Material
Cell & Tissue Engineering
John P. Woods, R. Sathish Srinivasan, Lorin E. Olson
Summary: The study demonstrates how combining two recombinases can improve cell specificity in mice, enhancing lineage tracing and gene inactivation.
Article
Biology
Katherine A. Kiwimagi, Justin H. Letendre, Benjamin H. Weinberg, Junmin Wang, Mingzhe Chen, Leandro Watanabe, Chris J. Myers, Jacob Beal, Wilson W. Wong, Ron Weiss
Summary: Kiwimagi & Letendre et al. present a workflow to quantitatively define recombinase-based digitizer and predict responses to different input signals. With a mechanistic/phenotypic model that can predict circuit performance, they generate a synthetic cell-cell communication device that amplifies a synNotch output signal.
COMMUNICATIONS BIOLOGY
(2021)
Article
Virology
Nannan Liu, Chenjian Gu, Yang Yang, Zixiang Gao, Ning Kang, Jing Liu, Youhua Xie
Summary: This study reports a method for the generation of circular viral genomes through DNA recombination in cells. It allows for simpler manipulation of the BKV genome and results in higher virus yield.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biology
Helene Duplus-Bottin, Martin Spichty, Gerard Triqueneaux, Christophe Place, Philippe Emmanuel Mangeot, Theophile Ohlmann, Franck Vittoz, Gael Yvert
Summary: LiCre is a novel light-inducible Cre recombinase, capable of being activated within minutes by blue light with faster and stronger activation effects and lower residual activity in the dark. It shows high efficiency in both yeast and human cells, making it suitable for fundamental research, biomedical research, and industrial bioprocess control.
Article
Immunology
Agata N. Rumianek, Ben Davies, Keith M. Channon, David R. Greaves, Gareth S. D. Purvis
Summary: In this study, a novel transgenic mouse (hCD68-CreERT2) was successfully generated to target macrophages in vivo under continuous tamoxifen induction, without affecting the expression of other myeloid cells. Through mating with the tdTomato variant mouse, the efficiency and specificity of this transgenic mouse were validated.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Haiyan Ren, Anqi Yin, Pingping Wu, Huanyu Zhou, Jungang Zhou, Yao Yu, Hong Lu
Summary: In this study, the positions and sequences of centromeres in Kluyveromyces marxianus were determined for the first time, and a Cre-loxP system was successfully established in this species. A K. marxianus strain containing 16 loxPsym sites was constructed, and large-scale chromosomal rearrangements were achieved with a high recombination frequency. These findings provide valuable information and tools for studying chromosomal structures and functions in K. marxianus.
Article
Biochemistry & Molecular Biology
Sumiyo Morita, Takuro Horii, Mika Kimura, Ryosuke Kobayashi, Hiroki Tanaka, Hidetaka Akita, Izuho Hatada
Summary: Knockout mice are important tools for studying the normal function of genes. Liver-specific conditional knockout mice can be generated using lipid nanoparticles, which offers a more convenient method compared to traditional approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ruben Dario Castro-Torres, Jordi Olloquequi, Miren Etchetto, Pablo Caruana, Luke Steele, Kyra-Mae Leighton, Jesus Urena, Carlos Beas-Zarate, Antoni Camins, Ester Verdaguer, Carme Auladell
Summary: This study investigated the impact of dual genetic deletion of Mkk4/Mkk7 on hippocampal neurogenesis in adult mice. It was found that the levels of MKK4/MKK7 were not completely eliminated in the CNS after gene deletion, leading to alterations in hippocampal structure and maturation process. These data support the role of the MKK4/MKK7-JNK pathway in adult neurogenic activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Rahul Kumar, Yun Mao, Sonika Patial, Yogesh Saini
Summary: This study investigated the effect of different Tamoxifen (TAM) regimens on whole-body gene deletion in mice using Tamoxifen-inducible Cre recombinase. The results demonstrated that a combination of TAM-diet with either TAM-injections or TAM-oral gavage showed the most efficient deletion of genes in the whole body.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Peng-Yang Liu, Gang Li, Chu-Bin Lin, Jun-Jie Wu, Shan Jiang, Feng-Hong Huang, Xia Wan
Summary: By establishing a seamless genome editing system, we successfully enhanced the production of astaxanthin in Schizochytrium sp., broadening the application of this strain.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
R. Akbari Moghaddam Kakhki, D. W. L. Ma, K. R. Price, J. Moats, N. A. Karrow, E. G. Kiarie
Summary: The study examined the effects of feeding sources of n-3 fatty acids to ISA Brown and Shaver White breeders and their offspring on antibody titres and plasma FA profile. Results showed that feeding breeders FFF increased plasma concentration of antibody titres and n-3 FA, while DMA reduced plasma concentration of Sigma n-6 FA in offspring. Overall, alpha linolenic acid (ALA) and docosahexaenoic acid (DHA) sources demonstrated varied responses regardless of breeder strain.
BRITISH POULTRY SCIENCE
(2021)
Article
Public, Environmental & Occupational Health
Lisa Tang, Julia Broad, Rebecca Lewis, David W. L. Ma, Jess Haines
Summary: This study examined the experiences and strategies of Health Educators as they transitioned from a home-based model to remote delivery during COVID-19. Key learnings included using online video platforms, providing necessary information and technical support, building rapport, and asking probing questions to elicit deeper reflection.
PATIENT EDUCATION AND COUNSELING
(2021)
Article
Nutrition & Dietetics
Cody A. C. Lust, Xinyan Bi, Christiani Jeyakumar Henry, David W. L. Ma
Summary: The study analyzed individual fatty acid reference ranges in 476 middle-aged Singaporean males and females, finding that females had significantly higher concentrations in four types of fatty acids. Participants with fatty acid concentrations above the 95th percentile also showed high risk levels for triglycerides, HDL, LDL, and total cholesterol, indicating potential cardiovascular disease risk.
Article
Nutrition & Dietetics
Ennaliza Salazar, Sultan Alenezi, Katherine J. P. Schwenger, Sarah Casselman, Nicha Somlaw, Patricia Kim, Daniela Adjemian, Zihang Lu, Wendy Lou, David Ma, Johane P. Allard
Summary: In this study, it was found that olive oil-based intravenous lipid emulsion (IVLE) was not inferior to soybean oil-based IVLE in maintaining or increasing prealbumin levels in patients receiving parenteral nutrition in the acute-care setting. Additionally, improvements in C-reactive protein levels and other clinical outcomes were not significantly different between the two groups.
Article
Nutrition & Dietetics
Valerie Hruska, Nitin Shivappa, James R. Hebert, Alison M. Duncan, Jess Haines, David W. L. Ma
Summary: This study investigated the link between family-based stress and dietary patterns that promote inflammation. It found that chaotic and dysfunctional family environments were associated with more proinflammatory dietary profiles in parents, indicating an increased risk of chronic disease. No direct association was found between children's dietary inflammatory profiles and family stress, suggesting the need for further research to explore possible indirect connections through family food-related behaviors.
Article
Nutrition & Dietetics
Melissa Gonzalez-Soto, Salma A. Abdelmagid, David W. L. Ma, Ahmed El-Sohemy, David M. Mutch
Summary: Previous research demonstrated that soy consumption, compared to dairy consumption, is associated with reduced overall desaturation pathway activity and specifically suppresses D6D activity. Grouping participants based on soy and dairy intake habits revealed that individuals consuming soy (with or without dairy) had significantly lower levels of omega-6 LC-PUFAs and various estimates of desaturase pathway activity compared to those consuming only fluid milk and dairy products. Soy consumption appears to have a stronger impact on desaturase pathway activity than dairy consumption in this study.
Article
Multidisciplinary Sciences
Cody A. C. Lust, Xinjie Lin, Erin M. Rock, Cheryl L. Limebeer, Linda A. Parker, David W. L. Ma
Summary: This pilot study examined the distribution of cannabinoids in different tissues of Sprague-Dawley rats and found significant differences in concentration. THC was most concentrated in the jejunum and adipose tissue, while the brain had the lowest concentration. The liver contained the highest concentration of THC metabolites. These findings emphasize the need to consider the bioactive effects of cannabinoids in peripheral tissues in future research.
Article
Education & Educational Research
Hannah J. Coyle-Asbil, David W. L. Ma, Lori Ann Vallis
Summary: This study examined the impact of applying an autocalibration algorithm on accelerometer data using different signal processing techniques and how it altered the signal composition. The results showed that calibration correction had a significant effect on ENMO, VM lowpass, and VM data, but minimal impact on VM highpass and bandpass data. When comparing across the techniques, ENMO yielded the lowest outputs, followed by VM highpass/VM bandpass, and then VM lowpass/VM which produced very similar results.
MEASUREMENT IN PHYSICAL EDUCATION AND EXERCISE SCIENCE
(2022)
Article
Nutrition & Dietetics
Anisha Mahajan, Jess Haines, Alex Carriero, Jaimie L. Hogan, Jessica Yu, Andrea C. Buchholz, Alison M. Duncan, Gerarda Darlington, David W. L. Ma
Summary: There is limited research on the intake of non-nutritive sweeteners (NNS) among preschool-aged children. This study aimed to investigate the frequency and type of NNS intake in preschool-aged children and found that a portion of the children consumed NNS through foods and beverages, with sources including freezies and oral nutritional supplements.
Article
Nutrition & Dietetics
Jamie L. A. Martin, Nadia M. Cartwright, Amber L. Hutchinson, Lindsay E. Robinson, David W. L. Ma, Jennifer M. Monk
Summary: Short-chain fatty acids (SCFA) produced from dietary fermentation have metabolic effects in skeletal muscle, but their effect on inflammatory mediator production is unclear. This study found that SCFA have differential effects on inflammatory mediator expression in response to different stimulations, which has implications for their individual impacts on inflammation-mediated skeletal muscle dysfunction.
Article
Nutrition & Dietetics
Flora Zhang, Amar Laila, Ana Carolina Barco Leme, David W. L. Ma, Jess Haines
Summary: This study aimed to explore the knowledge and perceptions of the 2019 Canada's Food Guide (CFG) among children ages 9-12. The findings revealed that children lacked knowledge about certain foods emphasized in the CFG and expressed confusion about food groups. However, they generally had positive perceptions about the CFG and its eating habit recommendations, suggesting strategies to improve adherence to these recommendations.
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
(2022)
Review
Nutrition & Dietetics
Sandhya Sahye-Pudaruth, David W. L. Ma
Summary: There has been increased interest in the health benefits of n-3 polyunsaturated fatty acids (PUFA) in reducing chronic diseases. However, inconsistent findings in the literature call for a review of the evidence from randomized controlled trials (RCTs) in n-3 PUFA research. Factors such as varying doses and sources of n-3 PUFA, trial duration, and population characteristics may contribute to the inconsistency in the literature.
Article
Nutrition & Dietetics
Alyssa V. Ramuscak, Alison M. Duncan, David W. L. Ma, Guelph Family Hlth Study
Summary: This study found that Canadian parents have a general understanding of the recommendations in the 2019 Canada's Food Guide, but lack specific knowledge about the details, such as the names of the food groups and foods containing unhealthy fats.
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
(2023)
Article
Nutrition & Dietetics
Patricia F. C. Acosta, Olivia A. Landon, Zachary J. Ribau, Jess Haines, David W. L. Ma, Alison M. Duncan, Guelph Family Hlth Study
Summary: This study examined the plant-based food intake in preschool-aged children using the plant-based dietary index (PDI) metrics and found that higher PDI scores were associated with higher intakes of nutrients and food groups to encourage and lower intakes of those to limit. These results can inform dietetic practice for dietary guidance that promotes plant-based foods in children.
Article
Pediatrics
Anisha Mahajan, Jess Haines, Jessica Yu, Gerarda Darlington, Andrea C. Buchholz, Alison M. Duncan, David W. L. Ma
Summary: This study aimed to investigate the associations between sociodemographic characteristics and total, free, and added sugar intakes in young children. The results showed that child age, child ethnicity, and annual household income played crucial roles in shaping sugar intake in young children. This research provides valuable insights for public health policy and nutrition interventions, emphasizing the need for early behavior interventions to reduce sugar intake in young children while considering sociodemographic factors.