Article
Cell Biology
Peng Cheng, Shengjie Bao, Chengxiang Li, Jianhua Tong, Lisha Shen, Hao Yu
Summary: This study discovered the deposition mechanism of m6A RNA modification in rice, which stabilizes the OsYUCCA3 mRNA and promotes auxin synthesis during anther development. The OsFAP1-OsFIP37 mediated m6A modification is crucial for meiotic division and subsequent pollen development in rice.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Jian-Ping Lian, Yu-Wei Yang, Rui-Rui He, Lu Yang, Yan-Fei Zhou, Meng-Qi Lei, Zhi Zhang, Jia-Hui Huang, Yu Cheng, Yu-Wei Liu, Yu-Chan Zhang, Yue-Qin Chen
Summary: MEL1, a rice AGO protein, is ubiquitinated and degraded by a monocot-specific E3 ligase XBOS36 post-meiosis, leading to semi-sterility if abnormally accumulated. Inhibition of MEL1 degradation disrupts pollen formation at the microspore stage. This study provides insight into the interaction between a monocot-specific E3 ligase and an AGO protein during plant reproductive development.
Article
Multidisciplinary Sciences
Yafeng Zheng, Donghui Wang, Sida Ye, Wenqian Chen, Guilan Li, Zhihong Xu, Shunong Bai, Feng Zhao
Summary: This study reveals the crucial role of auxin in guiding GC specification in Arabidopsis anthers. Additionally, it demonstrates that the segregation of GC from somatic cells involves a dynamic feedback circuit among local auxin biosynthesis, transcription of GC determinant protein, and progressive GC specification.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Lini Sethi, Debasish Deb, Badrinath Khadanga, Nrisingha Dey
Summary: Researchers analyzed the synthetic full-length transcript promoter of Blueberry red ringspot virus (BRRV) and developed two chimeric promoters (MBR3 and FBR3) with transcriptional activities equivalent to that of the CaMV35S(2) promoter. These chimeric promoters showed high antimicrobial activities against several pathogens, making them potential useful substitutes for the CaMV35S(2) promoter in plant genetic engineering.
Article
Plant Sciences
Woo-Jong Hong, Su Kyoung Lee, Seok-Hui Kim, Yu-Jin Kim, Sunok Moon, Eui-Jung Kim, Jeniffer Silva, Ki-Hong Jung
Summary: In this study, we conducted transcriptome analysis to understand the regulatory mechanism for anther dehiscence in rice. Through functional classification, we identified hormone signaling and transcriptional regulation pathways as important factors in anther dehiscence and anther wall development. These findings provide valuable insights into the molecular mechanisms of anther dehiscence and anther wall development in rice for future research.
PLANT CELL REPORTS
(2022)
Article
Agronomy
Yao Deng, Yingchun Wan, Weichi Liu, Lisha Zhang, Kai Zhou, Ping Feng, Guanghua He, Nan Wang
Summary: The OsFLA1 gene plays a crucial role in the male reproductive development in rice, and its mutation may result in pollen abortion and male sterility.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Developmental Biology
Jekaterina Truskina, Sophy Boeuf, Joan Renard, Tonni Grube Andersen, Niko Geldner, Gwyneth Ingram
Summary: During flowering plant reproduction, the tapetum and developing pollen grains are symplastically isolated from each other, and from other sporophytic tissues, by a structure called the peritapetal strip. This strip prevents the diffusion of substances from outside the cells. The formation and barrier functions of the peritapetal strip depend on two NADPH oxidases.
Article
Biochemical Research Methods
Yang Li, Liping Yu, Qiushuang Wang, Xiangyu Zhao, Xinzheng Li, Baoxiu Qi
Summary: The study identified a 523bp DNA fragment in Arabidopsis that can replace the original 1653bp promoter to drive similar temporal and embryo-specific expression of GUS, which could be used in metabolic engineering for producing valuable pharmaceutical products in seeds.
PLANT MOLECULAR BIOLOGY REPORTER
(2021)
Article
Plant Sciences
Nan Wu, Junduo Li, Qiaoling Zheng, Jieping Wu, Weirong Xu
Summary: This study evaluated the expression differences of the HOS1 gene in different genotypes of cold-tolerant grapes, and found significant differences in the length and promoter sequences of the HOS1 gene between the two grape types. The deletion of a 313-bp region abolished the promoter activity of VvHOS1. Additionally, potential regulatory proteins were identified to interact with the 313-bp core promoter region and regulate GUS gene expression.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Yang Zhao, Lijuan Xu, Yuanxiang Huang, Hongying Wu, Xingen Zhang, Xiaolin Hu, Qing Ma
Summary: This study conducted a systematic analysis of the Di19 gene family in maize, revealing that the ZmDi19-5 gene exhibits significant drought stress responsive characteristics. The study identified the promoter region and upstream regulatory proteins associated with stress response, laying an important foundation for further understanding the role of this gene family in plant drought response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuta Hiragori, Hiro Takahashi, Taihei Karino, Atsushi Kaido, Noriya Hayashi, Shun Sasaki, Kodai Nakao, Taichiro Motomura, Yui Yamashita, Satoshi Naito, Hitoshi Onouchi
Summary: This study identified four novel non-AUG-initiated upstream open reading frames (uORFs) with evolutionarily conserved sequences in Arabidopsis and elucidated the mechanism by which a non-AUG-initiated uORF promotes the translation of the main open reading frame (mORF). Through a combination of bioinformatics and experimental analysis, regulatory non-AUG uORFs that are physiologically important were identified and their effects on mORF expression were examined. These findings provide insights into the mechanisms that enable non-AUG uORFs to play regulatory roles despite their low translation initiation efficiencies.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Sudthana Khlaimongkhon, Sriprapai Chakhonkaen, Keasinee Tongmark, Numphet Sangarwut, Natjaree Panyawut, Thiwawan Wasinanon, Kannika Sikaewtung, Samart Wanchana, Chareerat Mongkolsiriwatana, Julapark Chunwonges, Amorntip Muangprom
Summary: Rice is a crucial food crop for nearly half of the world's population, and its grain yields are affected by temperature changes. Thermosensitive genic male sterility (TGMS) rice is sterile at high temperatures and fertile at low temperatures, making it a good model for studying the effects of temperature on male development. Transcriptomic analysis revealed differential gene expression in TGMS rice panicles under sterile and fertile conditions, with some genes showing unique responses to temperature in TGMS plants compared to wild-type rice. These findings provide insights into the molecular mechanisms controlling TGMS and rice responses to temperature alterations.
Article
Agronomy
Cuihong Huang, Jian Zhang, Danhua Zhou, Yuting Huang, Ling Su, Guili Yang, Wenlong Luo, Zhiqiang Chen, Hui Wang, Tao Guo
Summary: A novel QTL qCIR9.1 controlling callus induction rate in anther culture was identified in rice, with Os09g0551600 being the most promising candidate gene based on RNA-seq data. This study mapped QTLs responsible for anther culturability and found a major QTL named qCIR9.1 on chromosome 9. RNA-seq analysis revealed potential candidate genes and provided a foundational step for further cloning qCIR9.1 to improve anther culturability in rice.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Plant Sciences
Jing-Shi Xue, Chi Yao, Qin-Lin Xu, Chang-Xu Sui, Xin-Lei Jia, Wen-Jing Hu, Yong-Lin Lv, Yi-Feng Feng, Yu-Jia Peng, Shi-Yi Shen, Nai-Ying Yang, Yu-Xia Lou, Zhong-Nan Yang
Summary: A live imaging technique for observing anther development was established in this study, revealing the initiation, development, and degradation of the middle layer in Arabidopsis through marker lines and staining methods.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Tsheten Sherpa, Deepak Kumar Jha, Khushbu Kumari, Jeky Chanwala, Nrisingha Dey
Summary: We characterized the efficient chimeric sub-genomic transcript promoter FHS4 from Horseradish Latent Virus, which is active in both dicot and monocot plants, and could be a potential tool for plant biotechnology.
Article
Plant Sciences
Naveen Malik, Rajeev Ranjan, Swarup K. Parida, Pinky Agarwal, Akhilesh K. Tyagi
Article
Multidisciplinary Sciences
Priyanka Deveshwar, Shivam Sharma, Ankita Prusty, Neha Sinha, Sajad Majeed Zargar, Divya Karwal, Vishal Parashar, Sanjeev Singh, Akhilesh Kumar Tyagi
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Jitender Giri, Swarup K. Parida, Saurabh Raghuvanshi, Akhilesh K. Tyagi
Summary: Rice production requires a significant amount of water and is vulnerable to drought. To ensure sustainable rice cultivation with limited water resources, research focuses on strategies and genetic manipulation to improve water use efficiency and drought tolerance.
Article
Genetics & Heredity
Ankit Verma, Geeta Prakash, Rajeev Ranjan, Akhilesh K. Tyagi, Pinky Agarwal
Summary: The study reveals that OsGW2 acts as a negative regulator of grain size in indica rice by affecting both cell number and cell size in the spikelet hull. This is achieved through downregulation of OsGW2 by RNAi technology, resulting in wider and heavier grains. Transcriptome analysis identified upregulated genes related to grain size regulation in an OsGW2_RNAi transgenic line among 1,426 differentially expressed genes.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Bipin K. Pandey, Lokesh Verma, Ankita Prusty, Ajit Pal Singh, Malcolm J. Bennett, Akhilesh K. Tyagi, Jitender Giri, Poonam Mehra
Summary: OsJAZ11 regulates phosphate homeostasis by suppressing jasmonic acid signaling and biosynthesis in rice roots, promoting root elongation and enhancing phosphate uptake. This study highlights the functional connections between JA and phosphate signaling, demonstrating JAZ repressors as a promising candidate for improving low phosphate tolerance in elite rice genotypes.
Article
Plant Sciences
Ridhi Khurana, Sanchi Bhimrajka, Gundra Sivakrishna Rao, Vibha Verma, Neelima Boora, Gautam Gawande, Meenu Kapoor, Khareedu Venkateswara Rao, Sanjay Kapoor
Summary: OsMADS29 is a seed-specific transcription factor involved in embryo and endosperm development and starch filling during seed development in rice. The upstream regulatory region of OsMADS29 contains both positive and negative regulatory elements, as well as auxin-responsive elements, which guide its development-dependent and spatial expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Poonam Mehra, Bipin K. Pandey, Lokesh Verma, Ankita Prusty, Ajit Pal Singh, Shivam Sharma, Naveen Malik, Malcolm J. Bennett, Swarup K. Parida, Jitender Giri, Akhilesh K. Tyagi
Summary: Seed size is an important determinant of seed weight and crop yield, and the jasmonic acid (JA) signaling repressor OsJAZ11 plays a key role in controlling rice seed width and weight. Overexpressing OsJAZ11 in transgenic rice lines resulted in wider and heavier seeds, as well as altered spikelet morphogenesis and expression of JA-related genes, OsGW7, and MADS genes.
Article
Biochemistry & Molecular Biology
Rajeev Ranjan, Naveen Malik, Shivam Sharma, Pinky Agarwal, Sanjay Kapoor, Akhilesh K. Tyagi
Summary: The study found that OsCPK29 is an important regulator of pollen development in rice. Silencing of OsCPK29 leads to reduced fertility, decreased seed set, and non-germinating collapsed pollen grains. OsCPK29 regulates intine development through its interaction with the MADS68 transcription factor. These findings are significant for understanding the pollen development mechanism in rice.
Article
Plant Sciences
Anurag Daware, Ankit Malik, Rishi Srivastava, Durdam Das, Ranjith K. K. Ellur, Ashok K. K. Singh, Akhilesh K. K. Tyagi, Swarup K. K. Parida
Summary: The advent of the pangenome era has uncovered previously unknown genetic variation in diverse crop plants, but conventional single-reference genotyping methods often fail to capture a large portion of this variation, leading to reference bias. To overcome this, a Rice Pangenome Genotyping Array (RPGA) was developed, enabling rapid genotyping based on the entire 3K rice pangenome. This array provides a cost-effective solution and has been successfully applied in various areas, such as genome-wide association studies, population structure analysis, and marker-assisted selection.
Article
Plant Sciences
Virevol Thakro, Naveen Malik, Udita Basu, Rishi Srivastava, Laxmi Narnoliya, Anurag Daware, Nidhi Varshney, Jitendra K. Mohanty, Deepak Bajaj, Vikas Dwivedi, Shailesh Tripathi, Uday Chand Jha, Girish Prasad Dixit, Ashok K. Singh, Akhilesh K. Tyagi, Hari D. Upadhyaya, Swarup K. Parida
Summary: Identifying a bHLH transcription factor, CabHLH10, and its related genes in chickpea provides a potential molecular approach for breeding drought-tolerant and high-yielding cultivars.
Article
Biology
Arunima Mahto, Antima Yadav, P. V. Aswathi, Swarup K. Parida, Akhilesh K. Tyagi, Pinky Agarwal
Summary: By analyzing the transcriptomes and miRNomes of small-grained and large-grained indica rice, this study identified the crucial role of the S3 stage in enhancing grain size and discovered several transcription factor and miRNA families that influence grain size. A Domino effect model was proposed to explain the regulation of grain size. Additionally, a rice grain development database was established for easy access to the data generated in this study.
Review
Plant Sciences
Ankita Prusty, Shivam Sharma, Naveen Malik, Sanjay Kapoor, Akhilesh Kumar Tyagi
Summary: The Mediator complex in plants, encoded by multiple paralogous genes, regulates various aspects of plant growth and development through a complex network. It plays critical roles in shaping plant architecture, responding to hormonal signals, maintaining metabolite homeostasis, and producing non-coding RNAs.
CRITICAL REVIEWS IN PLANT SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Vibha Verma, Neelima Boora, Saraswati Nayar, Gulshan Kumar, Somesh Thapliyal, Ankur, Ridhi Khurana, Gautam Gawande, Meenu Kapoor, Sanjay Kapoor
Summary: OsMADS29 (M29) is a crucial regulator of seed development in rice and its expression is tightly regulated at transcriptional and post-transcriptional levels. This study reveals that calmodulin (CaM) interacts with M29 in a Ca2+-dependent manner, leading to localization of M29 in the cytoplasm. The interaction of CaM with M29 may also facilitate dimerization of two M29 monomers, suggesting a general regulatory mechanism for oligomerization and nuclear transport of MADS proteins.
Review
Genetics & Heredity
Priyanka Deveshwar, Ankita Prusty, Shivam Sharma, Akhilesh K. Tyagi
FRONTIERS IN GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Shuang Li, Saraswati Nayar, HuiYuan Jia, Sanjay Kapoor, Juan Wu, Yasushi Yukawa
