Article
Multidisciplinary Sciences
Gheyath K. Nasrallah, Raniya Al-Buainain, Nadin Younes, Soha R. Dargham, Duaa W. Al-Sadeq, Mohamed Elhassan, Ibrahim Al-Shaar, Hadi M. Yassine, Laith J. Abu-Raddad, Mohamed M. Emara, Ahmed Ismail
Summary: HIV and Syphilis are common STIs that pose a concern and burden on healthcare systems. This study evaluated the diagnostic efficiency of the protocols used by the Medical Commission Department (MC) in Qatar for screening these infections.
Article
Chemistry, Analytical
Kiara Lee, John Murphy, Anubhav Tripathi
Summary: This study developed a DBS extraction method using the synergistic action of electrophoretic and diffusive transport mechanisms to extract high-quality gDNA in just 5 minutes, without the need for heat, shaking, and DNA purification steps. The method is suitable for point of care and automation.
ANALYTICAL CHEMISTRY
(2022)
Article
Virology
Kosuke Kosai, Norihito Kaku, Michiko Horie, Hina Kodama, Norihiko Akamatsu, Yusuke Narita, Yasushi Matsumoto, Tetsuro Matsushita, Yohei Mizuta, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara
Summary: The cobas 6800 system and cobas SARS-CoV-2 & Influenza A/B demonstrated high accuracy in detecting influenza A virus and can be valuable for clinical laboratories that need to routinely test a large number of samples.
Article
Multidisciplinary Sciences
Parisa Shiri, Veronica Lai, Tara Zepel, Daniel Griffin, Jonathan Reifman, Sean Clark, Shad Grunert, Lars P. E. Yunker, Sebastian Steiner, Henry Situ, Fan Yang, Paloma L. Prieto, Jason E. Hein
Summary: Solubility screening is essential but labor intensive. By developing a flexible robotic system with computer vision and iterative feedback, researchers can successfully measure solubility in multiple solvents autonomously, saving time and resources.
Article
Virology
Fan Yang, Yixin Xiao, Fumin Liu, Hangping Yao, Nanping Wu, Haibo Wu
Summary: A rapid detection method for H7N9 avian influenza virus (AIV) was established using monoclonal antibodies against hemagglutinin (HA) to develop an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA). AC-ELISA showed high specificity and sensitivity, with a detection limit of 3.9 ng/mL for H7N9 HA protein and 2(-2)HA unit/100 mu L for live H7N9 AIV. The technique proved to be rapid and practical for H7N9 AIV detection, with a sensitivity of 94.96% and specificity of 88.24% compared to conventional virus isolation detection.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemical Research Methods
Miriam Klausberger, Nikolaus F. Kienzl, Gerhard Stadlmayr, Clemens Grunwald-Gruber, Elisabeth Laurent, Katharina Stadlbauer, Florian Stracke, Klemens Vierlinger, Manuela Hofner, Gabriele Manhart, Wilhelm Gerner, Florian Grebien, Andreas Weinhausel, Lukas Mach, Gordana Wozniak-Knopp
Summary: This study aimed to optimize the receptor binding domain (RBD) of the SARS-CoV-2 spike protein produced in HEK293-6E cells. By modifying the O-glycosylation site and removing the unpaired cysteine residue, the researchers achieved a more stable and monomeric form of RBD. These optimized RBD variants showed excellent performance in detecting SARS-CoV-2 antibodies in convalescent sera, indicating their potential for serological testing and vaccine development.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Lise Barbe, Stephanie Lam, Austin Holub, Zohreh Faghihmonzavi, Minnie Deng, Rajshri Iyer, Steven Finkbeiner
Summary: DNA damage is a common cellular feature in cancer and neurodegenerative disease, but there is a lack of fast and accurate methods for quantifying DNA damage. The comet assay is a biochemical tool that measures DNA damage based on the migration of broken DNA strands towards a positive electrode, creating a quantifiable 'tail' behind the cell. However, the analysis of comets using existing open-source algorithms is time-consuming and requires manual curation. To overcome these limitations, we developed AutoComet, an open-source algorithm that automates comet segmentation and quantification of tail parameters. AutoComet significantly improves segmentation accuracy and reduces analysis time over ten-fold, providing a fast and unbiased method for quantifying DNA damage.
Article
Multidisciplinary Sciences
Chengxi Li, Alex J. Callahan, Mark D. Simon, Kyle A. Totaro, Alexander J. Mijalis, Kruttika-Suhas Phadke, Genwei Zhang, Nina Hartrampf, Carly K. Schissel, Ming Zhou, Hong Zong, Gunnar J. Hanson, Andrei Loas, Nicola L. B. Pohl, David E. Verhoeven, Bradley L. Pentelute
Summary: The study introduces the development of an automated flow synthesis method that significantly reduces nucleobase coupling times from hours to minutes, enabling high-throughput production and eliminating human errors.
NATURE COMMUNICATIONS
(2021)
Article
Medical Laboratory Technology
Sumi Yoon, Hee-Won Moon, Hanah Kim, Mina Hur, Yeo-Min Yun
Summary: Two fully automated immunoassays for antinuclear antibody (ANA) screening, EliA CTD Screen and QUANTA Flash CTD Screen Plus, showed reliable performance compared with the indirect immunofluorescence assay (IIFA) and can be efficiently used in clinical immunology laboratories. Clinical cut-off values can be adjusted according to the workflow in each laboratory.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Agricultural Engineering
Jiafeng Yuan, Guanben Du, Hongxing Yang, Sichen Liu, Seongsu Park, Tongda Liu, Xin Ran, Byung-Dae Park, Wei Gao, Long Yang
Summary: Lignin was functionalized to obtain quinonoid lignin (QL) and epoxidized lignin (EL), and cellulose was modified to be aminated cellulose (AC). A novel fully bio-based adhesive of QL-AC-EL with multiple cross-linked networks was designed based on Schiff base and addition reaction. The bonding performance was significantly improved due to the covalent crosslinking between adhesive and activated wood interface, resulting in enhanced water resistance of the interfacial bonding stability. This work provides new inspiration for the design of bio-based adhesives with excellent performance and water resistance from the interfacial chemical bonding perspective.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Infectious Diseases
Sebastian Hin, Benjamin Lopez-Jimena, Mohammed Bakheit, Vanessa Klein, Seamus Stack, Cheikh Fall, Amadou Sall, Khalid Enan, Mohamed Mustafa, Liz Gillies, Viorel Rusu, Sven Goethel, Nils Paust, Roland Zengerle, Sieghard Frischmann, Manfred Weidmann, Konstantinos Mitsakakis
Summary: The study presents a LabDisk platform for differential diagnosis of acute fever caused by various pathogens, demonstrating promising results for detecting tropical diseases and precise identification of the cause of acute fever.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biophysics
Chunling Mao, Lei Wu, Yi Wen, Ximing Tang, Zheng Huang, Longshan Zhao
Summary: A photoelectrochemical immunosensor based on MIL-101(Cr) and CdTe-QDs composites was synthesized to detect the carcinoembryonic antigen (CEA) in human serum and urine samples. The sensor demonstrated altered photocurrent polarity, improved light utilization efficiency, and enhanced separation efficiency of electron-hole pairs. Under optimized conditions, the sensor showed high sensitivity and stability, with detection limits of 0.00018 ng mL-1 and 0.00023 ng mL-1 for anodic and cathodic detection of CEA, respectively.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Adane Adugna, Gebereselassie Demeke, Milkiyas Toru, Dareskedar Tsehay, Ahmed Esmael, Adane Mihret, Andargachew Mulu
Summary: This study evaluated the protective efficacy of hepatitis B vaccine and estimated the seroprevalence of hepatitis B virus infection among vaccinated children. The results showed an intermediate prevalence of childhood HBV infection despite vaccination, suggesting a low protective efficacy of the hepatitis B vaccine in the study area.
Article
Biochemistry & Molecular Biology
Katja F. Hellendahl, Maryke Fehlau, Sebastian Hans, Peter Neubauer, Anke Kurreck
Summary: The study introduces a luciferase-based assay for the detection of NK activity, which was validated in a 96-well plate format. The high-throughput assay showed comparable results to HPLC analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Agriculture, Dairy & Animal Science
Xun He, Shan Wang, Caoyuan Ma, Guang-Ri Xu, Jinyou Ma, Hongbing Xie, Wei Zhu, Hongyang Liu, Lei Wang, Yimin Wang
Summary: The detection of animal viruses is a challenging task with significant practical implications. Early diagnosis of animal virus infections is crucial for preventing disease spread and reducing economic losses. Electrochemical sensors have emerged as promising tools for rapid diagnosis, offering benefits such as sensitivity, affordability, usability, portability, and real-time analysis. This review focuses on the construction and advantages of electrochemical biosensors for animal virus detection, highlighting their potential as a research direction.