Journal
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
Volume 103, Issue 7, Pages 698-702Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.trstmh.2009.02.018
Keywords
Plasmodium falciparum; Fagara zanthoxyloides; Pseudocedrela kotschyi; Schizonts; Trophozoites; 50% inhibitory concentration
Funding
- NIH [A144857-04, UHI-HLO367905]
- National Institute of Allergy and Infectious Diseases
- National Heart, Lung and Blood Institute
- Office of Research on Minority Health
- Howard University General Clinical Research Center [MOI-RR10284]
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Roots of Pseudocedrela kotschyi are commonly used as chewing sticks in West Africa. This study examined the effects of the plant extract on the in-vitro growth of Plasmodium falciparum. Ring-stage synchronised cultures of the malaria parasite were exposed to 30 and 60 mu g/ml of P kotschyi extract for 51 h. Aliquots were taken from the cultures every 3 h for preparation of Giemsa-stained thin films, which were evaluated by light microscopy for degree of parasitaemia and stage distribution of parasite development. The extracts did not show any inhibitory effects on the emergence of trophozoites in treated cultures. However, the results indicate that 80% of inhibition of the parasite transformation into schizont was obtained for both tested concentrations (30 and 60 mu g/ml). Experiments with (3)H-hypoxanthine incorporation showed an IC(50) of 16 mu g/mL for the Pseudocedrela extract. Pseudocedrela was combined with extract of Fagara zanthoxyloides in various concentrations to determine their interactive effects on the in-vitro cultures. Isobologram analysis of the results indicated a synergistic interaction between the two extracts at low concentrations, while interactions at higher concentrations showed antagonistic effects. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
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