Article
Biology
Anthony Santella, Irina Kolotuev, Caroline Kizilyaprak, Zhirong Bao
Summary: Analyzing brain development, structure, and function across imaging modalities can provide valuable information. This study combines electron microscopy and time-lapse fluorescence microscopy to study the emergence of the nervous system in Caenorhabditis elegans embryogenesis.
Article
Optics
Jiajia Chen, Zhenhong Du, Chenhao Xu, Xiao Xiao, Wei Gong, Ke Si
Summary: Here we report an ultrafast 3D histological imaging scheme based on optimized selective plane illumination microscopy (mSPIM), a minutes-time scale clearing method (FOCM), and a deep learning-based image enhancement algorithm (SRACNet) to realize histological preparation and imaging of clinical tissues. Our scheme enables fast imaging at micron-level resolution and demonstrates detailed 3D morphological connections that are difficult to identify in conventional 2D histology.
Article
Multidisciplinary Sciences
Ayana Sugizaki, Keisuke Sato, Kazuyoshi Chiba, Kenta Saito, Masahiko Kawagishi, Yuri Tomabechi, Shalin B. Mehta, Hirokazu Ishii, Naoki Sakai, Mikako Shirouzu, Tomomi Tani, Sumio Terada
Summary: Biomolecular assemblies play a crucial role in cell physiology, with their function depending on the changes in molecular positions and orientations. Fluorescence polarization microscopy offers a promising solution by reporting the orientation of emission dipoles, providing insights into monitoring molecular orientation in real time within living cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Claudia Matthaeus, Kem A. Sochacki, Andrea M. Dickey, Dmytro Puchkov, Volker Haucke, Martin Lehmann, Justin W. Taraska
Summary: This study investigates the key proteins that regulate the curvature of caveolae using correlative super-resolution light and electron microscopy. The authors discover that caveolins, cavins, and EHD2 form a cohesive structural unit, regulated by intermittent associations with Pacsin2 and EHBP1, to control the curvature of caveolae.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Alexandre Chappard, Craig Leighton, Rebecca S. S. Saleeb, Kiani Jeacock, Sarah R. R. Ball, Katie Morris, Owen Kantelberg, Ji-Eun Lee, Elsa Zacco, Annalisa Pastore, Margaret Sunde, David J. J. Clarke, Patrick Downey, Tilo Kunath, Mathew H. H. Horrocks
Summary: Protein misfolding and aggregation are common features of neurogenerative disorders. Single-molecule techniques have allowed the characterization of these protein aggregates, previously inaccessible with traditional methods. In this study, we employed a high affinity antibody labeled with orthogonal fluorophores, along with fast-flow microfluidics and single-molecule confocal microscopy, to specifically detect alpha-synuclein in biologically relevant samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Optics
Maryam Baker, Euan McLeod
Summary: Fluorescence and photoluminescence can be used for high contrast imaging through the use of specific probes, but bulky microscopes require mechanical scanning and stitching for high space-bandwidth product images. Lensfree imaging can achieve a high space-bandwidth product through computational reconstruction from diffraction patterns. In this study, a time-gated lensfree photoluminescent imaging system with high resolution was developed using europium chelate fluorophores and temporal filtering.
Article
Multidisciplinary Sciences
Chenfei Hu, Shenghua He, Young Jae Lee, Yuchen He, Edward M. Kong, Hua Li, Mark A. Anastasio, Gabriel Popescu
Summary: The study introduces a method for assessing cell viability without the need for labeling, utilizing deep learning techniques combined with quantitative phase imaging to accurately determine cell survival status without causing damage to the cells.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Thomas Kellerer, Janko Janusch, Christian Freymueller, Adrian Ruehm, Ronald Sroka, Thomas Hellerer
Summary: This study utilized two different fluorescence lifetime measurement techniques to investigate the factors affecting fluorescence lifetime results, showing consistent results and revealing the strongest influencing factor on fluorescence lifetime. It also provides quantitative guidance on how to choose the most suitable technique and perform experiments correctly to obtain consistent fluorescence lifetime results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Bolong Zhang, Kieran D. Richards, Beatrice E. Jones, Abigail R. Collins, Rosie Sanders, Sarah R. Needham, Pu Qian, Amoghavarsha Mahadevegowda, Caterina Ducati, Stanley W. Botchway, Rachel C. Evans
Summary: This study demonstrates the first example of triplet-triplet annihilation upconversion (TTA-UC) based lifetime imaging microscopy. Ultra-small nanoparticle (NP) probes were used to overcome background autofluorescence and achieve excellent image contrast and oxygen mapping in living cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Nanoscience & Nanotechnology
Yuan Luo, Ming Lun Tseng, Sunil Vyas, Ting-Yu Hsieh, Jui-Ching Wu, Shang-Yang Chen, Hsiao-Fang Peng, Vin-Cent Su, Tzu-Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia-Wern Chen, Yu-Chun Chen, Kuang-Yuh Huang, Chieh-Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai
Summary: Light-sheet fluorescent microscopy (LSFM) is the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. This study demonstrates the successful integration of a nanophotonic meta-lens as the illumination component for LSFM, addressing the challenges of high image resolution and optical sectioning. With the meta-lens, the LSFM system complexity is significantly reduced, enabling multicolor fluorescent imaging of live biological specimens with cellular resolution.
Article
Cell Biology
Sandeep M. Nalluri, Chinmay S. Sankhe, Joseph W. O'Connor, Paul L. Blanchard, Joelle N. Khouri, Steven H. Phan, Gage Virgi, Esther W. Gomez
Summary: Epithelial-mesenchymal transition (EMT) is a physiological process essential for embryogenesis and wound healing, but also implicated in pathologies like fibrosis and cancer. Regulation of phosphorylation status can impact cell physiology and gene expression changes during TGFβ1-induced EMT, highlighting the crucial role of intricate crosstalk between signaling cascades in regulating EMT processes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Optics
Rui Jin, Yalan Yu, Dan Shen, Qingming Luo, Hui Gong, Jing Yuan
Summary: A flexible, video-rate, and defocus-aberration-compensated axial dual-line scanning imaging method is proposed to simultaneously observe biological structures and activities in different views of interest. The method allows flexible adjustment of the positions of two fields of view and the optical sectioning effects according to specific experimental requirements.
PHOTONICS RESEARCH
(2021)
Article
Cell Biology
Eriko Hamasaki, Natsuki Wakita, Hiroki Yasuoka, Hikaru Nagaoka, Masayuki Morita, Eizo Takashima, Takayuki Uchihashi, Tetsuya Takeda, Tadashi Abe, Ji-Won Lee, Tadahiro Iimura, Moin A. Saleem, Naohisa Ogo, Akira Asai, Akihiro Narita, Kohji Takei, Hiroshi Yamada
Summary: This study investigates the molecular mechanisms underlying the regulation of actin by dynamin in human podocytes. The results suggest that the affinity of the K562E mutant for lipid membranes and its atypical self-assembling properties lead to actin disorganization in podocytes. Furthermore, the interaction between actin and membranes mediated by dynamin is critical for actin bundle formation and podocyte morphology and functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Shan Gao, Mengzhou Li, Jason T. Smith, Xavier Intes
Summary: We report on a novel time-domain mesoscopic fluorescence molecular tomography (TD-MFMT) system designed for multiplexed molecular imaging in turbid media, demonstrating high sensitivity and specificity. Phantom studies and ex vivo tumor imaging validate the system's potential for imaging deep targets and resolving intra-tumoral heterogeneity.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Multidisciplinary
Cheng Ma, Zejing Xing, Xiaodan Gou, Li-Ping Jiang, Jun-Jie Zhu
Summary: This study develops a temperature-tuned ECL layer to image single cell topography at different heights by regulating the thickness of the ECL layer with electrode temperature. The ECL layer in two routes shows different regulation ranges with elevated temperature, providing a flexible approach to adjust the imaging scope within specific heights. Moreover, a heated electrode significantly improves the image quality of cell adhesion in heterogeneous electrochemical rate-determined situations. This work offers a new approach to regulate the ECL layer and has promising applications in monitoring transient heat generation from biological entities.
Article
Biochemistry & Molecular Biology
Mario Schiffer, Beina Teng, Changkyu Gu, Valentina A. Shchedrina, Marina Kasaikina, Vincent A. Pham, Nils Hanke, Song Rong, Faikah Gueler, Patricia Schroder, Irini Tossidou, Joon-Keun Park, Lynne Staggs, Hermann Haller, Sergej Erschow, Denise Hilfiker-Kleiner, Changli Wei, Chuang Chen, Nicholas Tardi, Samy Hakroush, Martin K. Selig, Aleksandr Vasilyev, Sandra Merscher, Jochen Reiser, Sanja Sever
Review
Biochemistry & Molecular Biology
Valentina A. Shchedrina, Yan Zhang, Vyacheslav M. Labunskyy, Dolph L. Hatfield, Vadim N. Gladyshev
ANTIOXIDANTS & REDOX SIGNALING
(2010)
Article
Biochemistry & Molecular Biology
Anton A. Turanov, Valentina A. Shchedrina, Robert A. Everley, Alexei V. Lobanov, Sun Hee Yim, Stefano M. Marino, Steven P. Gygi, Dolph L. Hatfield, Vadim N. Gladyshev
BIOCHEMICAL JOURNAL
(2014)
Article
Biochemistry & Molecular Biology
Valentina A. Shchedrina, Hadise Kabil, Gerd Vorbruggen, Byung Cheon Lee, Anton A. Turanov, Mitsuko Hirosawa-Takamori, Hwa-Young Kim, Lawrence G. Harshman, Dolph L. Hatfield, Vadim N. Gladyshev
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Valentina A. Shchedrina, Robert A. Everley, Yan Zhang, Steven P. Gygi, Dolph L. Hatfield, Vadim N. Gladyshev
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Cell Biology
Valentina A. Shchedrina, Gerd Vorbrueggen, Byung Cheon Lee, Hwa-Young Kim, Hadise Kabil, Lawrence G. Harshman, Vadirn N. Gladyshev
MECHANISMS OF AGEING AND DEVELOPMENT
(2009)
Article
Biochemical Research Methods
Ikram Khan, Anil Prabhakar, Chloe Delepine, Hayley Tsang, Vincent Pham, Mriganka Sur
Summary: Organoids are biological systems grown in vitro that self-organize into 3D cellular tissues of specific organs, with brain organoids being valuable models for studying human brain development. Researchers are developing improved tools, such as microfluidic chips and bioreactors, to track and image brain organoids in real-time on a chip, enabling long-term live-imaging of the growing organoids with high resolution.
Article
Multidisciplinary Sciences
Chloe Delepine, Vincent A. Pham, Hayley W. S. Tsang, Mriganka Sur
Summary: Cerebral organoids generated from human pluripotent stem cells have the ability to recapitulate human-specific neurodevelopmental events, with the serine/threonine kinase GSK3 beta playing a critical role in these processes. Inhibition of GSK3 beta with low doses of CHIR 99021 can increase organoid size, but higher doses have the opposite effect, reducing organoid size and arresting growth. The effects of GSK3 beta inhibition on organoid development involve changes in cell survival, proliferation, differentiation, and migration, revealing new mechanisms for improving organoid production and understanding embryonic brain development.
Article
Biology
Murat Yildirim, Chloe Delepine, Danielle Feldman, Vincent A. Pham, Stephanie Chou, Jacque Ip, Alexi Nott, Li-Huei Tsai, Guo-Li Ming, Peter T. C. So, Mriganka Sur
Summary: This study demonstrates label-free three-photon imaging technology for assessing early events of early human brain development in whole, uncleared intact organoids. By imaging cerebral organoids generated from Rett Syndrome patients, the researchers found defects in the volumetric structure of the ventricular zone in mutant organoids compared to control organoids. Live imaging of organoids also revealed that mutant radially migrating neurons had shorter migration distances and slower migration speeds.
Article
Multidisciplinary Sciences
Valentina A. Shchedrina, Sergey V. Novoselov, Mikalai Yu. Malinouski, Vadim N. Gladyshev
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2007)
Article
Biochemistry & Molecular Biology
NL Klyachko, VA Shchedrina, AV Efimov, SV Kazakov, IG Gazaryan, BS Kristal, AM Brown
JOURNAL OF BIOLOGICAL CHEMISTRY
(2005)
Article
Cell Biology
JR Morgan, G Di Paolo, H Werner, VA Shckedrina, M Pypaert, VA Pieribone, P De Camilli
JOURNAL OF CELL BIOLOGY
(2004)
