Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 30, Issue 5, Pages 703-716Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2011.01.014
Keywords
Biomolecular mechanism; Heavy metal; Mass spectrometry; Multi-dimensional protein-identification technology (MudPIT); Protein expression; Protein marker; Proteomics; Toxicity; Two-dimensional gel electrophoresis (2-DE); Toxicological biomarker
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Funding
- Comunidad de Madrid (Analisyc II)
- Spanish Ministry of Science and Innovation [CTQ2008-01031, CTQ2010-18644]
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Examining the toxic effects of heavy metals on protein expression can be useful for gaining insight into the biomolecular mechanisms of toxicity and for identifying potential candidate metal-specific protein markers of exposure and response. In this article, we present the state of the art of proteomics in metal-toxicity-related studies. We consider different methods used for sample preparation that depend on the nature of the sample (plants, microorganisms and animals). We also describe different proteomic strategies, both gel-based and gel-free technologies, including two-dimensional gel electrophoresis (2-DE) and multidimensional protein-identification technology (MudPIT). We critically review the advantages and the disadvantages of such techniques and discuss the main studies carried out so far. We also comment on future applications and potential research interests within this field. (C) 2011 Elsevier Ltd. All rights reserved.
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