Article
Endocrinology & Metabolism
Wei Gao, Kornelius Schmidt, Soren Enge, Clemens Kirschbaum
Summary: The study measured hair endocannabinoid and N-acylethanolamine levels over a period of two and a half years in 100 female participants, with strong test-retest associations found and no correlations observed between perceived stress and the measured substances. The findings support the idea that endocannabinoids and N-acylethanolamines in hair are stable trait biomarkers over time.
PSYCHONEUROENDOCRINOLOGY
(2021)
Article
Biology
Csaba Varadi, Viktoria Hajdu, Flora Farkas, Ibolya Gilanyi, Csaba Olah, Bela Viskolcz
Summary: The study revealed significant differences in glycosylation patterns of patients with different brain tumors through quantitative analysis of serum N-glycosylation. Altered glycan structures could enhance the detection of malignancy.
Article
Pharmacology & Pharmacy
Amin Safdari, Farnaz Monajjemzadeh, Samin Hamidi
Summary: Famotidine, a commonly used medication for stomach acid-related disorders, has been found to contain carcinogenic impurities. The presence of NDMA in famotidine, regardless of brand or dosage, exceeds the permissible limit, highlighting the need for stricter control and monitoring to ensure drug safety.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dalma Dojcsak, Agnes Maria Ilosvai, Laszlo Vanyorek, Ibolya Gilanyi, Csaba Olah, Laszlo Horvath, Csaba Varadi
Summary: Glycosylation, essential for proper functioning of glycoproteins, is known to be altered in chronic inflammatory disorders such as multiple sclerosis. The use of magnetic nanoparticles in glycan sample preparation provides an easy-to-use solution for large-scale biomarker discovery studies. Analyzing serum samples from multiple sclerosis patients and healthy controls, significant glycosylation alterations were found, particularly in different gender groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Claudia Gimenez-Campillo, Marta Pastor-Belda, Natalia Campillo, Manuel Hernandez-Cordoba, Pilar Vinas
Summary: This study developed and validated an analytical method for the determination of nine NAs in ranitidine drug samples, highlighting the importance of developing analytical methods in detecting potential carcinogens in pharmaceutical products. The method showed quantification limits in the 0.21-21 ng g(-1) range, with satisfactory precision indicated by relative standard deviations lower than 12%.
Article
Biochemistry & Molecular Biology
Kazuhito Tsuboi, Tatsuya Tai, Ryouhei Yamashita, Hanif Ali, Takashi Watanabe, Toru Uyama, Yoko Okamoto, Keisuke Kitakaze, Yasuhiro Takenouchi, Shinji Go, Iffat Ara Sonia Rahman, Hitoshi Houchi, Tamotsu Tanaka, Yasuo Okamoto, Akira Tokumura, Junko Matsuda, Natsuo Ueda
Summary: This study demonstrates the ability of acid ceramidase (AC) to hydrolyze N-acylethanolamines (NAEs) and suggests its physiological role as a third NAE hydrolase.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemical Research Methods
Johannes B. Mueller-Reif, Fynn M. Hansen, Lisa Schweizer, Peter Treit, Philipp E. Geyer, Matthias Mann
Summary: Reversed-phase HPLC is commonly used in MS-based proteomics for peptide separation, with particle-packed capillary columns being predominantly used in nanoflow HPLC systems. Despite capillary columns being a standard for many years, they are expensive and have short lifetimes, leading many laboratories to produce their own in-house packed columns.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Chemistry, Analytical
Marina Bellot, Maria Espinosa-Velasco, Raul Lopez-Arnau, Elena Escubedo, Cristian Gomez-Canela
Summary: This study evaluated the performance of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the determination of multi-class monoamine neurotransmitters in mouse brain, and found significant differences in concentration levels of catecholamine neurotransmitters between brain regions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Multidisciplinary Sciences
Melissa Simard, Andrea Tremblay, Sophie Morin, Genevieve Rioux, Nicolas Flamand, Roxane Pouliot
Summary: This study aims to characterize the endocannabinoid system and evaluate the effects of n-3-derived NAEs on psoriatic keratinocytes. The study found that n-3 polyunsaturated fatty acids can alter the levels of related lipid mediators, reduce keratinocyte proliferation, and alleviate the inflammatory features of psoriasis.
SCIENTIFIC REPORTS
(2023)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Luyao Liu, Bin Zhu, Zheng Fang, Na Zhang, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Zhenzhen Yao, Mingliang Ye
Summary: Bottom-up proteomics is increasingly used in clinical research, but glycoproteomic analysis has been a bottleneck. An automated glycopeptide enrichment method was developed for site-specific N-glycoproteome analysis, showing higher efficiency and reliability.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
S. M. Khaledur Rahman, Zahir Hussain, Katsuya Morito, Naoko Takahashi, Mohammad Mamun Sikder, Tamotsu Tanaka, Ken-ichi Ohta, Masaki Ueno, Hiroo Takahashi, Tohru Yamamoto, Makoto Murakami, Toru Uyama, Natsuo Ueda
Summary: N-Acyl-phosphatidylethanolamines (NAPEs) and their bioactive metabolites (NAEs) accumulate during ischemia. The enzyme cPLA(2)epsilon is responsible for the formation of NAPEs during ischemia.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Agriculture, Multidisciplinary
Garret Couture, Devanand L. Luthria, Ye Chen, Nikita P. Bacalzo Jr, Fakir S. Tareq, James Harnly, Katherine M. Phillips, Pamela R. Pehrsson, Kyle McKillop, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: Dietary fiber is essential for a healthy diet and plays a significant role in the gut microbiome-health connection. Current analytical methods for food carbohydrate analysis do not provide structural information about fiber. To address this gap, researchers developed a new analytical pipeline using rapid-throughput LC-MS methods to determine the structural composition of fiber fractions in different foods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Xueshu Li, Marco M. Hefti, Rachel F. Marek, Keri C. Hornbuckle, Kai Wang, Hans-Joachim Lehmler
Summary: This study investigated the distribution of PCBs and OH-PCBs in the human brain, finding higher levels of PCBs in adult brains and significant contributions of lower chlorinated PCBs to neurotoxicity in some donors.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Malgorzata Tyszka-Czochara, Marzena Suder, Agnieszka Dolhanczuk-Srodka, Malgorzata Rajfur, Katarzyna Grata, Michal Starosta, Agnieszka Jagoda-Pasternak, Wiktor Kasprzyk, Anna K. Nowak, Saeid Ahmadzadeh, Dorota Kopec, Piotr Surylo, Tomasz Swiergosz, Katarzyna M. Stadnicka
Summary: Innovative engineering design for biologically active hydroxyapatites requires enhancing their mechanical and physical properties, as well as biocompatibility, by doping with appropriate chemical elements. This investigation evaluated the model of naturally occurring hydroxyapatite and the effects of doped trace elements on normal human fibroblasts. The results showed that a moderate amount of fluoride ions had a positive effect on cell proliferation, while chloride ions had an inhibitory effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Patryk Szymaszek, Pawel Fiedor, Anna Chachaj-Brekiesz, Malgorzata Tyszka-Czochara, Tomasz Swiergosz, Joanna Ortyl
Summary: This study investigated the interaction between selected pyridine derivatives and bovine serum albumin (BSA) using UV-Vis and fluorescence spectroscopy. The results showed that the probes led to a significant increase in emission intensity, and the calculated binding parameters indicated that the probes bind to BSA with moderate strength in a 1:1 stoichiometry. The analysis also revealed that the interaction between the pyridine derivatives and BSA primarily occurs through van der Waals forces and hydrogen bonding. Furthermore, the study demonstrated that the evaluated probes can be safely used for BSA detection under physiological conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Pawel Grochecki, Irena Smaga, Karolina Wydra, Marta Marszalek-Grabska, Tymoteusz Slowik, Ewa Kedzierska, Joanna Listos, Ewa Gibula-Tarlowska, Malgorzata Filip, Jolanta H. H. Kotlinska
Summary: This study examined the complex issue of treating Post-Traumatic Stress Disorder (PTSD) when it coexists with drug use disorder. The results showed that conditioned fear, a model for PTSD, can alter the rewarding effect of mephedrone, and repeated injections of mephedrone can impact trauma-related behaviors such as fear sensitization, extinction, and recall. The study also found changes in the expression of metalloproteinase-9 (MMP-9) and GluN2A and GluN2B subunits of the N-methyl-D-aspartate (NMDA) glutamate receptor in the hippocampus and basolateral amygdala, suggesting the involvement of these brain structures in fear memory impairment and drug seeking behavior.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Patryk Szymaszek, Patrycja Sroda, Malgorzata Tyszka-Czochara, Anna Chachaj-Brekiesz, Tomasz Swiergosz, Joanna Ortyl
Summary: Here we report the synthesis of a novel fluorescent probe P182 for quantitative measurement of H2O2. This fluorometric probe displays a fluorescence turn-on response in the presence of H2O2. It offers good performances in terms of sensitivity and response time, and shows non-toxic activity for in vivo studies. This study provides research on molecular fluorescence probe for the detection of H2O2.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Pharmacology & Pharmacy
Joanna Jastrzebska, Malgorzata Frankowska, Irena Smaga, Magdalena Hubalewska-Mazgaj, Agata Suder, Renata Pieniazek, Edmund Przegalinski, Malgorzata Filip
Summary: The study revealed the key role of 5-HT2C receptors in treating CUD and comorbid depression and CUD, supporting further research into pharmacological strategies targeting these receptors.
PHARMACOLOGICAL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Wirginia Krzysciak, Paulina Karcz, Beata Bystrowska, Marta Szwajca, Amira Bryll, Natalia Smierciak, Anna Ligezka, Aleksander Turek, Tamas Kozicz, Anna E. Skalniak, Pawel Jagielski, Tadeusz J. Popiela, Maciej Pilecki
Summary: The altered cerebral energy metabolism in schizophrenia may be linked to lactate accumulation from gastrointestinal bacteria, which can cross the blood-brain barrier and cause brain acidity. This study found that lactate-utilising bacteria may represent a compensatory mechanism in response to increased lactate production in the brain. Assessment of neuronal function mediated by ALA-LAC-dependent NMDA regulatory mechanisms may support new therapies for schizophrenia, where acidosis has become a distinguishing feature.
Article
Behavioral Sciences
Irena Smaga, Kinga Gawlinska, Dawid Gawlinski, Paulina Surowka, Malgorzata Filip
Summary: Maternal high-fat diet during pregnancy and lactation disrupts short-term memory in adolescent and young adult females, likely due to dysregulation of glutamatergic system in the hippocampus. Changes in glutamatergic transporters and NMDA receptor subunits, AMPA receptor subunits, as well as BDNF levels may contribute to the behavioral changes observed in offspring.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Malgorzata Frankowska, Karolina Wydra, Agata Suder, Magdalena Zaniewska, Dawid Gawlinski, Joanna Miszkiel, Anna Furgala-Wojas, Kinga Salat, Malgorzata Filip, Christa E. Mueller, Katarzyna Kiec-Kononowicz, Magdalena Kotanska
Summary: This study discovered three novel selective ligands for GPR18, including one agonist and two antagonists. The activation of GPR18 has similar effects to the CB receptor system, impacting emotional behavior, food intake, and pain activity. Therefore, GPR18 represents a potential therapeutic target for mood, pain, and/or eating disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Bartosz Pomierny, Weronika Krzyzanowska, Alicja Skorkowska, Jakub Jurczyk, Beata Bystrowska, Boguslawa Budziszewska, Joanna Pera
Summary: Brain ischemia is a major cause of death and disability worldwide, and the release of glutamate after ischemia induces neuronal excitotoxicity. VGLUT1 and VGLUT2 are involved in filling presynaptic vesicles with glutamate. This study explores the effect of cerebral ischemia on the expression of VGLUT1 and VGLUT2, and the impact of VGLUT inhibition on glutamate release and stroke outcome.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Nutrition & Dietetics
Edmund Przegalinski, Kacper Witek, Karolina Wydra, Jolanta H. Kotlinska, Malgorzata Filip
Summary: Obesity is a significant health and economic issue, and the serotonin system, specifically the 5-HT2C receptors, plays a crucial role in regulating body weight. This review focuses on the use of 5-HT2C receptor agonists as antiobesity medications, such as fenfluramines, sibutramine, and lorcaserin, and discusses the potential of 5-HT2C receptor positive allosteric modulators as safer alternatives. More research is needed to determine the efficacy of these drugs in obesity prevention and treatment.
Article
Environmental Sciences
Sebastian Rojek, Ewa Poljanska, Weronika Chaim, Martyna Maciow-Glab, Beata Bystrowska
Summary: Legal highs pose a significant health risk, especially among individuals experimenting with psychoactive substances. Limited knowledge about the biotransformation of these substances hinders effective treatment in cases of intoxication. A comprehensive approach, involving in silico assessment, in vitro studies, and animal models, was used to trace the biotransformation of U-47700.
Article
Reproductive Biology
Zaneta Broniowska, Igor Tomczyk, Pawel Grzmil, Beata Bystrowska, Alicja Skorkowska, Alicja Maciejska, Grzegorz Kazek, Boguslawa Budziszewska
Summary: The study aimed to determine the effect of BP-2 on testicular function and investigate the role of the immune system in its toxicity. BP-2 was found to reduce sperm count and motility, increase abnormal sperm morphology, and decrease testosterone levels. The results suggest that the immunoactivation in the testes caused by BP-2 may contribute to its effects on testicular function.
REPRODUCTIVE TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Anna Adach, Malgorzata Tyszka-Czochara, Marek Daszkiewicz
Summary: Molecular crystals of monomeric and dimeric pyrazole complexes with good biological activities against cancer cells were prepared via one-pot syntheses. These complexes exhibited significant cytotoxicity for different cancer cell lines and showed potent anti-proliferative activity with great selectivity towards cancer cells. These findings are important for anticancer drug discovery.
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY
(2023)