Journal
TOXICOLOGY MECHANISMS AND METHODS
Volume 24, Issue 8, Pages 552-559Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/15376516.2014.951814
Keywords
Apoptosis; DNA damage; oxidative stress; p53; PM2.5
Categories
Funding
- Public Sector Program of National Environmental Protection of the People's Republic of China [201009008, 201409081]
- Capital Medical University Natural Science Foundation [2014ZR09]
- Guangxi Natural Science Foundation [2011GXNSFA018187]
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Epidemiological studies have shown that air pollution particulate matter (PM) is associated with increased respiratory morbidity and mortality. However, the mechanisms are not fully understood. Oxidative stress-mediated apoptosis plays an important role in the occurrence of respiratory diseases. In this study, human bronchial epithelial (16-HBE) cells were exposed to different concentrations (16-128 mg/ml) of PM2.5 for 24 h to investigate the apoptosis induced by PM2.5. The results showed that PM2.5 exposure significantly induced apoptosis, DNA strand breaks, and oxidative damage in a dose-dependent manner in 16-HBE cells. The expression of p53 and p73 increased significantly along with the dose of PM2.5 in 16-HBE cells, whereas the expression of p21(Cip1/WAF1) decreased; the expression of mdm2 increased and then decreased, but not significantly. Taken together, these observations indicate that PM2.5 may lead to oxidative damage and induce apoptosis through the p53-dependent pathway in 16-HBE cells. p53-dependent apoptosis mediated by DNA strand breaks may be an important mechanism of PM2.5-induced apoptosis in 16-HBE cells.
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