Article
Biotechnology & Applied Microbiology
Weian Du, Domenico Giosa, Junkang Wei, Letterio Giuffre, Ge Shi, Lamya El Aamri, Enrico D'Alessandro, Majida Hafidi, Sybren de Hoog, Orazio Romeo, Huaiqiu Huang
Summary: This study presents the annotated genome assemblies of four environmental Sporothrix species generated using PacBio SMRT sequencing data. The results show differences in genome size and gene content among these Sporothrix species, as well as differences in transposable elements and orthologous genes. Carbohydrate-active enzyme analysis reveals no significant differences between pathogenic and environmental species. The assembly of mitochondrial genomes is also reported. These findings provide a starting point for future studies on the diversification, ecological/host adaptation, and origin of pathogenic lineages within the genus Sporothrix.
Article
Microbiology
Remi Hatinguais, Ian Leaves, Gordon D. Brown, Alistair J. P. Brown, Matthias Brock, Roberta Peres da Silva
Summary: This study develops molecular tools for accurate genetic manipulation in Sporothrix species, addressing the paucity of reliable tools for stable genetic engineering. It is of great importance for studying the virulence determinants of sporotrichosis and their roles in disease establishment.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Mirelle Garcia Silva-Bailao, Patricia de Sousa Lima, Manoel Marques Evangelista Oliveira, Lua Cardoso Oliveira, Rodrigo Almeida-Paes, Clayton Luiz Borges, Alexandre Melo Bailao, Alexandre Siqueira Guedes Coelho, Celia Maria de Almeida Soares, Rosely Maria Zancope-Oliveira
Summary: This study used a nanoscale liquid chromatography coupled with tandem mass spectrometry approach to analyze the yeast proteomic profiles of S. brasiliensis, S. schenckii, and S. globosa, revealing differentially expressed proteins related to carbohydrate and amino acid metabolism. The findings suggest that S. brasiliensis has a distinct metabolism compared to S. schenckii and S. globosa, potentially explaining its more virulent character. The data provided new insights into Sporothrix species metabolism and may be useful for future functional studies.
MICROBES AND INFECTION
(2021)
Review
Microbiology
Jamile Ambrosio de Carvalho, Ruan Campos Monteiro, Ferry Hagen, Zoilo Pires de Camargo, Anderson Messias Rodrigues
Summary: Sporotrichosis, caused by thermodimorphic Sporothrix species, is a globally important mycosis with uneven distribution of etiological agents. Molecular diagnosis has gradually replaced traditional methods, and genotyping is crucial for epidemiological studies.
Article
Infectious Diseases
Paula Portella Della Terra, Fernanda Fidelis Gonsales, Jamile Ambrosio de Carvalho, Ferry Hagen, Rui Kano, Alexandro Bonifaz, Zoilo Pires de Camargo, Anderson Messias Rodrigues
Summary: Sporothrix schenckii and related species are causative agents of sporotrichosis, and traditional diagnostic methods may not be specific enough. The newly developed multiplex qPCR system can efficiently and directly detect Sporothrix DNA, which is essential for accelerating the diagnosis of sporotrichosis.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Dermatology
Li Lin, Junhao Huang, Junyue Fang, Jiahao Li, Wenying Cai, Jing Zhang, Sha Lu
Summary: This study classified 48 clinical isolates of sporothrix and tested their susceptibility to seven antifungal agents. The results showed that terbinafine and luliconazole were the most effective, followed by itraconazole and amphotericin B. Voriconazole, 5-flucytosine, and fluconazole had low efficacy. Overall, this study provides the first report on antifungal susceptibility and epidemiological correlation of sporothrix in southern China, and also the first evidence that sporothrix is sensitive to luliconazole.
Article
Infectious Diseases
Priscila Marques de Macedo, Aude Sturny-Leclere, Dayvison Francis Saraiva Freitas, Theo Ghelfenstein-Ferreira, Maria Clara Gutierrez-Galhardo, Marcos de Abreu Almeida, Anderson Messias Rodrigues, Thierry Pautet, Samia Hamane, Rodrigo Almeida-Paes, Rosely Maria Zancope-Oliveira, Alexandre Alanio
Summary: A new pan-Sporothrix quantitative reverse transcription PCR (RT-qPCR) assay was developed and validated on clinical samples from confirmed human sporotrichosis cases. This assay showed high sensitivity and specificity, providing a fast and reliable diagnostic tool for human sporotrichosis.
Article
Microbiology
Fernando Almeida-Silva, Marcos de Abreu Almeida, Vanessa Brito de Souza Rabello, Rosely Maria Zancope-Oliveira, Lilian Cristiane Baeza, Cristiane da Cruz Lamas, Marco Antonio Lima, Priscila Marques de Macedo, Maria Clara Gutierrez-Galhardo, Rodrigo Almeida-Paes, Dayvison Francis Saraiva Freitas
Summary: Sporotrichosis, a subcutaneous mycosis, can lead to several complications such as meningeal forms in immunocompromised individuals. However, the diagnosis of meningeal sporotrichosis is time-consuming due to the limitations of culture and the low fungal burden in cerebrospinal fluid (CSF) samples. This study evaluated five non-culture-based methods for detecting Sporothrix spp. in CSF and found that molecular and immunological tests had substantial sensitivity and specificity for indirect detection. Implementation of these methods in clinical practice could improve early detection, treatment optimization, and prognosis of affected individuals.
Review
Microbiology
Anderson Messias Rodrigues, Sarah Santos Goncalves, Jamile Ambrosio de Carvalho, Luana P. Borba-Santos, Sonia Rozental, Zoilo Pires de Camargo
Summary: Sporotrichosis, caused by Sporothrix species, is a significant implantation mycosis. Recent advancements in Sporothrix taxonomy have improved diagnosis, epidemiology, and treatment. Molecular epidemiology reveals the prevalence of S. brasiliensis in cat-transmitted outbreaks in South America, while S. globosa and S. schenckii cause major infections in Asia and the Americas, respectively. One-health approaches are necessary to address the spread of sporotrichosis. Speciating Sporothrix is vital in the 21st century, with PCR being the main method for molecular diagnosis. Treatment can be challenging due to resistance, but alternative drugs hold promise. This review explores these trends to mitigate the emerging and re-emerging disease.
Article
Veterinary Sciences
Raul Leal Faria Luiz, Rodrigo Caldas Menezes, Sandro Antonio Pereira, Raquel de Vasconcellos Carvalhaes de Oliveira, Manoel Marques Evangelista Oliveira
Summary: Sporotrichosis is a chronic fungal disease that affects humans and animals. This study aimed to evaluate and validate a nested PCR technique for detecting Sporothrix sp. DNA from formalin-fixed and paraffin-embedded tissues (FFPE). The results showed that the chemical extraction protocol with 5μm thick paraffin sections was the most effective in extracting DNA from Sporothrix sp. The nested PCR technique exhibited high sensitivity and specificity for detecting Sporothrix sp. in FFPE samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Danielly Correa-Moreira, Rodrigo C. Menezes, Orazio Romeo, Cintia M. Borba, Manoel M. E. Oliveira
Summary: By studying the pathological changes in mice infected with clinical and environmental isolates of different Sporothrix species, it was found that clinical S. schenckii isolate induced the most significant anatomopathological changes, suggesting that the pathogenicity of Sporothrix varies not only among species but also within different isolates of the same species.
Article
Microbiology
Fernando Almeida-Silva, Vanessa Brito de Souza Rabello, Bruno De Souza Scramignon-Costa, Rosely Maria Zancope-Oliveira, Priscila Marques de Macedo, Rodrigo Almeida-Paes
Summary: This study investigated 18 environmental samples collected from endemic areas of zoonotic sporotrichosis in Brazil, where domestic cats are scarce, and confirmed the presence of S. brasiliensis in the environment. The research highlights the challenges of nested-PCR in Sporothrix environmental studies, emphasizing the importance of sequencing to definitively identify the presence of Sporothrix spp. in environmental samples.
Review
Microbiology
Jorge A. Ortiz-Ramirez, Mayra Cuellar-Cruz, Julio C. Villagomez-Castro, Everardo Lopez-Romero
Summary: This article reviews the importance of fungal glycosidases in biological processes, with a particular focus on two human pathogens, Sporothrix species and C. albicans. The classification, substrates, mechanism of action, recent research advances, and potential biotechnological applications of glycosidases are discussed.
Article
Public, Environmental & Occupational Health
Max C. Ramirez-Soto, Elsa G. Aguilar-Ancori, Maria A. Quispe-Ricalde, Julia G. Muniz-Duran, Mercedes M. Quispe-Florez, Aldo Chinen
Summary: In this study, six clinical isolates of Sporothrix schenckii complex from Abancay, Peru were identified using PCR-sequencing of the calmodulin gene. The phylogenetic analysis revealed that these isolates clustered with sequences from other countries, indicating the presence of S. schenckii (sensu stricto) in Abancay, Peru.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Article
Immunology
Gabriela W. P. Neves, Sarah Sze Wah Wong, Vishukumar Aimanianda, Catherine Simenel, J. Inaki Guijarro, Catriona Walls, Janet A. Willment, Neil A. R. Gow, Carol A. Munro, Gordon D. Brown, Leila M. Lopes-Bezerra
Summary: This study investigated the immune response mechanisms of human against Sporothrix brasiliensis and Sporothrix schenckii. It was found that the interaction between these fungi and human cells relies on complement protein C3 and the peptidorhamnomannan (PRM) component of their cell walls. Blocking the CR3 receptor impacted the inflammatory response against these Sporothrix species.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biodiversity Conservation
Bruno Carneiro Rediguieri, Isabela da Cruz Bahiense, Jamile Ambrosio de Carvalho, Gustavo Rocha Leite, Aloisio Falqueto, Anderson Messias Rodrigues, Sarah Santos Goncalves
Summary: In this study, we investigated the clinical-epidemiological, microbiological, and molecular aspects of feline sporotrichosis in Espirito Santo, Brazil, and evaluated the reliability of cytopathological examination in diagnosis. The results showed that cytopathological examination demonstrated high agreement and accuracy, and there was an overwhelming occurrence of Sporothrix brasiliensis in the region.
Article
Mycology
Giannina Ricci, Emeline Boni Campanini, Angela Satie Nishikaku, Rosana Puccia, Mariangela Marques, Ralf Bialek, Anderson Messias Rodrigues, Wagner Luiz Batista
Summary: This study identified six phylogenetic lineages belonging to the P. brasiliensis complex, with two lineages not grouping with any of the four recognized species and a coinfection involving two lineages. Additionally, the study found five parsimony-informative sites with potential non-synonymous substitutions that could lead to changes in the protein.
Article
Infectious Diseases
Paula Portella Della Terra, Fernanda Fidelis Gonsales, Jamile Ambrosio de Carvalho, Ferry Hagen, Rui Kano, Alexandro Bonifaz, Zoilo Pires de Camargo, Anderson Messias Rodrigues
Summary: Sporothrix schenckii and related species are causative agents of sporotrichosis, and traditional diagnostic methods may not be specific enough. The newly developed multiplex qPCR system can efficiently and directly detect Sporothrix DNA, which is essential for accelerating the diagnosis of sporotrichosis.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Mycology
Anderson Messias Rodrigues, Ferry Hagen, Zoilo Pires de Camargo
Summary: Sporothrix is a genus of fungi with 53 species that can cause skin infections in humans and other mammals. Different species of Sporothrix follow different transmission strategies in epidemics, requiring distinct public health strategies to control the spread of sporotrichosis.
Article
Dermatology
Armando Guevara, Andreia Ferreira Nery, Marcia de Souza Carvalho Melhem, Lucas Bonfietti, Anderson Messias Rodrigues, Ferry Hagen, Jamile Ambrosio de Carvalho, Zoilo Pires de Camargo, Bruna Jacomel F. de Souza Lima, Vania Aparecida Vicente, Rosane Christine Hahn
Summary: Chromoblastomycosis primarily affects the poor population in Brazil, especially those involved in agricultural activities, with F. pedrosoi being the main etiological agent. Itraconazole, voriconazole, and terbinafine showed the highest antifungal activity.
Article
Mycology
Anderson Messias Rodrigues, Ferry Hagen, Rosana Puccia, Rosane Christine Hahn, Zoilo Pires de Camargo
Summary: Paracoccidioidomycosis (PCM) is a group of diseases caused by Paracoccidioides species. The genus has expanded to include seven species. Classic PCM affects the human lungs and may lead to systemic granulomatous disease, while PCM loboi and PCM ceti are observed as subcutaneous mycoses in humans and dolphins. Recognizing species boundaries in Paracoccidioides is crucial for understanding its ecology, evolution, clinical features, and strategies to mitigate PCM.
Article
Microbiology
Wdson Luis Lima Kruschewsky, Suzana Alves Patricio, Isabela Cruz Bahiense, Simone Bravim Maifrede, Ana Paula Possa, Zoilo Pires de Camargo, Anderson Messias Rodrigues, Paulo Mendes Pecanha, Aloisio Falqueto, Tania Regina Grao-Velloso, Sarah Santos Goncalves
Summary: This study evaluated the clinicopathological characteristics of ten patients diagnosed with PCM caused by P. lutzii. The study emphasizes the importance of using different antigens in testing patients with clinical manifestations of PCM and negative serological tests for P. brasiliensis.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Letter
Infectious Diseases
Bram Spruijtenburg, Amanda Bombassaro, Eelco F. J. Meijer, Anderson Messias Rodrigues, Maria Eduarda Grisolia, Vania Aparecida Vicente, Flavio de Queiroz-Telles, Jacques F. Meis, Theun de Groot
JOURNAL OF INFECTION
(2023)
Article
Microbiology
Luiza Chaves de Miranda Leonhardt Losada, Ruan Campos Monteiro, Jamile Ambrosio de Carvalho, Ferry Hagen, Matthew C. Fisher, Bram Spruijtenburg, Jacques F. Meis, Theun de Groot, Sarah Santos Goncalves, Ricardo Negroni, Rui Kano, Alexandro Bonifaz, Zoilo Pires de Camargo, Anderson Messias Rodrigues
Summary: This study developed a panel of highly polymorphic SSR markers suitable for genotyping Sporothrix species. PCR amplification revealed 240 alleles in 180 Sporothrix isolates, supporting the effectiveness of SSR markers in uncovering cryptic genetic diversity. The analysis of SSR diversity provided insights into the origins and transmission dynamics of cat-transmitted sporotrichosis.
Article
Microbiology
Breno Goncalves Pinheiro, Ana Paula Possa, Giannina Ricci, Angela Satie Nishikaku, Ferry Hagen, Rosane Christine Hahn, Zoilo Pires de Camargo, Anderson Messias Rodrigues
Summary: Classic paracoccidioidomycosis (PCM) is a potentially deadly tropical systemic mycosis caused by Paracoccidioides species. A new multiplex probe-based qPCR assay was developed for the detection and speciation of Paracoccidioides. The assay showed high specificity and sensitivity in detecting PCM in clinical samples. This study contributes to the improvement of early diagnosis and treatment of PCM.
Correction
Microbiology
Wdson Luis Lima Kruschewsky, Suzana Alves Patricio, Isabela Cruz Bahiense, Simone Bravim Maifrede, Ana Paula Possa, Zoilo Pires de Camargo, Anderson Messias Rodrigues, Paulo Mendes Pecanha, Aloisio Falqueto, Tania Regina Grao-Velloso, Sarah Santos Goncalves
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Infectious Diseases
Priscila Marques de Macedo, Aude Sturny-Leclere, Dayvison Francis Saraiva Freitas, Theo Ghelfenstein-Ferreira, Maria Clara Gutierrez-Galhardo, Marcos de Abreu Almeida, Anderson Messias Rodrigues, Thierry Pautet, Samia Hamane, Rodrigo Almeida-Paes, Rosely Maria Zancope-Oliveira, Alexandre Alanio
Summary: A new pan-Sporothrix quantitative reverse transcription PCR (RT-qPCR) assay was developed and validated on clinical samples from confirmed human sporotrichosis cases. This assay showed high sensitivity and specificity, providing a fast and reliable diagnostic tool for human sporotrichosis.
Article
Dermatology
Salene Angelini Colombo, Gustavo Canesso Bicalho, Camila Stefanie Fonseca de Oliveira, Danielle Ferreira de Magalhaes Soares, Lauranne Alves Salvato, Kelly Moura Keller, Camila de Valgas e Bastos, Maria Helena Franco Morais, Anderson Messias Rodrigues, Joao Luis Reis Cunha, Maria Isabel de Azevedo
Summary: This study aimed to identify the Sporothrix species involved in the ongoing outbreaks of animal sporotrichosis in the metropolitan region of Belo Horizonte, Brazil, and analyze their genetic relationships. The study found a significant increase in animal cases, with cats being the most affected host. Sporothrix brasiliensis was identified as the predominant agent, with evidence of circulation of different genotypes associated with animal sporotrichosis in the region.
Article
Microbiology
Sarah Santos Goncalves, Isabela da Cruz Bahiense Rocha, Bruno Carneiro Rediguieri, Jamile Ambrosio de Carvalho, Simone Bravim Maifrede, Wdson Luis Lima Kruschewsky, Aloisio Falqueto, Anderson Messias Rodrigues
Summary: This study investigates the genetic variations, molecular epidemiology significance, and transmission modes of Sporothrix species associated with sporotrichosis outbreaks in Espirito Santo, Brazil. The isolates were identified based on morphological and molecular characteristics, and antifungal susceptibility testing was performed. The predominant species during outbreaks was S. brasiliensis, with a significant role of domestic cats in its emergence. AFLP results showed intraspecific variability among S. brasiliensis isolates, suggesting multiple emergences. Terbinafine showed the best results in vitro, but itraconazole remains the primary treatment choice. Containing the disease and preventing future epidemics require one-health strategies.
Article
Mycology
Bert Gerrits van den Ende, Anderson Messias Rodrigues, Rosane Christine Hahn, Ferry Hagen
Summary: This article reports a case of chronic multifocal paracoccidioidomycosis in a Venezuelan patient. Whole genome sequencing revealed that the isolated strain was actually a Nannizziopsis species. This finding highlights the need for molecular characterization of cases that clinically mimic paracoccidioidomycosis but are serologically negative for Paracoccidioides.
REVISTA IBEROAMERICANA DE MICOLOGIA
(2023)