Article
Chemistry, Applied
Shaofeng Wu, Dan Zhou, Furong Geng, Jianyu Dong, Lebin Su, Yongbo Zhou, Shuang-Feng Yin
Summary: A simple and green method for the synthesis of benzoxazoles from catechols, aldehydes, and ammonium acetate using NaIO4 as an oxidant under metal- and additive-free conditions has been developed. The protocol results in high yields of benzoxazoles with excellent functional group tolerance, with mechanistic investigations revealing the involvement of a unique intermediate.
ADVANCED SYNTHESIS & CATALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Jin-Hee Kim, Jimin Lee, Hyesoo Jeong, Mi Seo Bang, Jin-Hyun Jeong, Minsun Chang
Summary: The study investigated the novel biological activities of NDGA in relation to COMT and how COMT inhibition by NDGA affects 4-OHE2-induced cyto- and genotoxicity in MCF-7 human breast cancer cells. The results showed that NDGA both inhibits COMT-mediated formation of 4-MeOE2 and increases 4-OHE2-induced DNA damage and cytotoxicity, potentially increasing catechol estrogen-induced genotoxicities in mammary tissues.
Article
Polymer Science
Matthew A. Bartucci, Alice M. Savage, David Flanagan, Brian F. Morgan, Frederick L. Beyer, Scott C. Radzinski, Joshua A. Orlicki, Joseph L. Lenhart
Summary: The study investigated the performance of a series of PAM copolymers in adhesive applications, finding little correlation between the polarity and thermal properties of the polymers and the observed adhesive strength. The study also highlighted the importance of processing conditions and conditioning in evaluating novel polymer compositions.
POLYMER INTERNATIONAL
(2021)
Article
Environmental Sciences
Manoswini Chakrabarti, Anita Mukherjee
Summary: This study evaluated the Cd-induced adaptive response in plants to mitigate the genotoxicity of EMS. Priming plants with Cd decreased the genotoxicity of EMS and the adaptive response was evident in cytotoxicity, genotoxicity, and biochemical parameters. The involvement of metabolic pathways and epigenetic modifications in AR was investigated, showing the contribution of DNA hypermethylation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Pharmacology & Pharmacy
Verena Krauth, Ferdinando Bruno, Simona Pace, Paul M. Jordan, Veronika Temml, Maria Preziosa Romano, Haroon Khan, Daniela Schuster, Antonietta Rossi, Rosanna Filosa, Oliver Werz
Summary: 5-Lipoxygenase (LO) is a key enzyme in the formation of pro-inflammatory leukotrienes (LT) that are important mediators in allergic reactions and inflammatory disorders. In this study, modifications were made to catechol derivatives to increase their effectiveness as selective 5-LO inhibitors. Two compounds, 3,4-dihydroxy-3 '-phenoxybiphenyl (6b) and 2-(3,4-dihydroxyphenyl)benzo[b]thiophene (6d), showed potent inhibition of human 5-LO in both cell-free and cell-based assays. Furthermore, these compounds were effective in inhibiting 5-LO in human blood and in a murine peritonitis model. These novel catechol derivatives have high selectivity and effectiveness as inhibitors of 5-LO under pathophysiological conditions.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuan Yue, Likun Ren, Chao Zhang, Kai Miao, Kun Tan, Qianying Yang, Yupei Hu, Guangyin Xi, Gang Luo, Mingyao Yang, Jingyu Zhang, Zhuocheng Hou, Lei An, Jianhui Tian
Summary: This study reveals the dynamic process of mtDNA methylation during the critical developmental window and its role in maintaining mitochondrial genome stability. The researchers also discovered that DNA methyltransferase 3A and DNMT3B catalyze de novo mtDNA methylation and bind to mtDNA through their unique mitochondrial targeting sequences. The results demonstrate that increased mtDNA methylation functions to protect the mitochondrial genome against damage induced by mitochondrial oxidative stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Sneha Damal Villivalam, Scott M. Ebert, Hee Woong Lim, Jinse Kim, Dongjoo You, Byung Chul Jung, Hector H. Palacios, Tabitha Tcheau, Christopher M. Adams, Sona Kang
Summary: Exercise can significantly increase the expression of DNMT3A in oxidative red muscle, playing a pivotal role in endurance exercise by controlling intracellular oxidative stress. Deficiency of DNMT3A leads to reduced tolerance to endurance exercise, which can be rescued by inhibiting the ALDH1L1 pathway.
Article
Environmental Sciences
Na Li, Jinhan Wang, Kejun Li, Ping Yang, Yan Wang, Chang Xu, Ningning He, Kaihua Ji, Huijuan Song, Manman Zhang, Liqing Du, Qiang Liu
Summary: This study found that long-term exposure to e-waste can lead to changes in DNA methylation levels, which can further impact health. By testing the blood samples of e-waste recycling workers and a control group, it was discovered that the concentration of 28 PCB congeners in the blood samples of e-waste recycling workers was higher, and DNA damage levels were significantly higher compared to the control group. Additionally, it was also found that exposure to e-waste potentially changes the methylation levels of 1233 gene loci, which could have negative effects on health.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Changjiang Fan, Xinyue Meng, Wei Yang, Peiyan Wang, Wenguang Chang, Peifeng Li, Jianxun Wang
Summary: A labeling chemistry-based method, APSC-8-oxoGua-seq, is developed for sequencing 8-oxoGua in the microRNA transcriptome. The method uses N-(3-azidopropyl)-spermine-5-carboxamide (APSC) for selective labeling of 8-oxoGua, allowing the conjugation of a cleavable linker via the click reaction for 8-oxoGua pull-down and sequencing. APSC-8-oxoGua-seq enables identification of 8-oxoGua at single-base resolution.
CHEMICAL COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Andrea Polli, Jolien Hendrix, Kelly Ickmans, Jelena Bakusic, Manosij Ghosh, Dora Monteyne, Brigitte Velkeniers, Bram Bekaert, Jo Nijs, Lode Godderis
Summary: The study found associations between COMT haplotypes and DNA methylation, cytokine expression, and symptoms in patients with CFS/FM, but not specific to one condition. Significant differences were observed in increased DNA methylation in the MB-COMT promoter and decreased IFN-gamma expression in patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Pei-Chi Chiang, Pei-Tzu Li, Ming-Jen Lee, Chin-Tin Chen
Summary: This study demonstrates that the reduced cellular invasion induced by photodynamic therapy (PDT) is associated with CLIC4 expression suppression through increased DNMT1 expression and promoter hypermethylation, involving the tumor suppressor p53. The findings further support that the regulation of CLIC4 expression in tumorigenesis is mediated by DNMT1-mediated hypermethylation and depends on the status of p53.
Article
Chemistry, Medicinal
Cijo George Vazhappilly, Rawad Hodeify, Shoib Sarwar Siddiqui, Amina Jamal Laham, Varsha Menon, Raafat El-Awady, Rachel Matar, Maxime Merheb, John Marton, Hussain Abdel Karim Al Zouabi, Rajan Radhakrishnan
Summary: The study found that catechol exhibited concentration-dependent cytotoxicity and anti-proliferative effects in breast cancer cells, while showing minimal effects on noncancerous cells. Furthermore, catechol activated the ATM/ATR pathways to induce DNA damage and cell death mechanisms through regulation of cell cycle proteins. Overall, catechol demonstrated a favorable safety profile for noncancerous cells and targeted multiple signaling cascades to inhibit breast cancer cell proliferation.
PHYTOTHERAPY RESEARCH
(2021)
Article
Endocrinology & Metabolism
Bing Song, Yujie Chen, Chao Wang, Guanjian Li, Zhaolian Wei, Xiaojin He, Yunxia Cao
Summary: This study suggests that abnormal DNA methylation of certain CpG sites of imprinted genes is associated with poor sperm quality and severe sperm DNA damage.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Michel Fasnacht, Norbert Polacek
Summary: Since the great oxidation event, Earth's cellular life forms have had to deal with reactive oxygen species (ROS) affecting biomolecules and cellular metabolism. Increased ROS levels in the biosphere represent growing stress and shape species evolution. Systems have evolved to remove ROS and repair damage, but oxidative stress occurs if ROS surpass the cell's capacity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Iwona Bukowska-Osko, Dorota Sulejczak, Katarzyna Kaczynska, Patrycja Kleczkowska, Karol Kramkowski, Marta Popiel, Ewa Wietrak, Pawel Kowalczyk
Summary: Structural abnormalities can cause DNA modifications, leading to various diseases including cancer. Cells defend and repair damaged genetic material through lactoferrin, an endogenous protein with antibacterial, antiviral, antifungal, and anticancer properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
