Journal
TOXICOLOGY IN VITRO
Volume 28, Issue 5, Pages 856-865Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2014.02.012
Keywords
Silicon carbide powders; Acheson process; Thermal post-treatment; Physico-chemical characterization; Biological toxicity; Occupational exposure
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Funding
- French Ministry of Industry
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Silicon carbide (SiC) an industrial-scale product manufactured through the Acheson process, is largely employed in various applications. Its toxicity has been poorly investigated. Our study aims at characterizing the physico-chemical features and the in vitro impact on biological activity of five manufactured SiC powders: two coarse powders (SiC C1/C2), two fine powders (SiC F1/F2) and a powder rich in iron impurities (SIC I). RAW 264.7 macrophages were exposed to the different SiC particles and the cellular responses were evaluated. Contrary to what happens with silica, no SiC cytotoxicity was observed but pro-oxidative and pro-inflammatory responses of variable intensity were evidenced. Oxidative stress (H2O2 production) appeared related to SiC particle size, while iron level regulated pro-inflammatory response (TNF alpha, production). To investigate the impact of surface reactivity on the biological responses, coarse SiC C1 and fine SiC F1 powders were submitted to different thermal treatments (650-1400 degrees C) in order to alter the oxidation state of the particle surface. At 1400 degrees C a decrease in TNF alpha production and an increase in HO center dot-, COO center dot- radicals production were observed in correlation with the formation of a surface layer of crystalline silica. Finally, a strong correlation was observed between surface oxidation state and in vitro toxicity. (C) 2014 Elsevier Ltd. All rights reserved.
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