Article
Cell Biology
Yuhua Chen, Kai Gong, Limin Guo, Bingchang Zhang, Sifang Chen, Zhangyu Li, Xu Quanhua, Wei Liu, Zhanxiang Wang
Summary: Research has shown that overexpression of Pgam5 post-TBI can worsen physiological and neurological outcomes, while depletion of Pgam5 can improve microglia activation, neuron damage, tissue lesion, and neurological dysfunction. Pgam5 may regulate inflammatory responses and affect protein expression of related genes in TBI.
CELL DEATH DISCOVERY
(2021)
Review
Chemistry, Inorganic & Nuclear
Yudong Wu, Wujun Chen, Chao Wang, Dongming Xing
Summary: Alkaline phosphatase (ALP) is a zinc-containing metalloprotein widely distributed in mammalian tissues that plays a vital role in clinical diagnosis. It is widely used for signal amplification in enzyme-linked assays and is a promising biomarker for disease diagnosis. Assays using L-ascorbic acid 2-phosphate (AAP) as a substrate have been developed to detect ALP activity, providing new insights into the construction of advanced sensing platforms for clinical diagnosis.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Jing Lu, Daoxiang Li, Lele Ma, Mingsan Miao, Yanju Liu, Jinming Kong
Summary: A novel fluorescence assay for alkaline phosphatase (ALP) activity detection is proposed using the activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) strategy. The method exhibited excellent performance in the detection of fluorescence signals.
Article
Chemistry, Analytical
Lei Deng, Feng Huang, Aomei Zhang, Tingting Wang, Minghui Yang, Xiaoqing Li, Xiang Chen
Summary: A fluorescent sensor for alkaline phosphatase determination based on CsPbBr3/BSA NCs perovskite was successfully developed by using bovine serum albumin (BSA) as a zwitterion ligand. The sensor showed a linear concentration range of 50 to 500 μM for L-ascorbic acid (AA) and a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, as well as a linear concentration range of 40 to 500 U/L for alkaline phosphatase (ALP) and a detection limit of 15.5 U/L (signal-to-noise ratio of 3) for ALP. Furthermore, the proposed biosensor exhibited good selectivity and recovery in the determination of ALP in human serum.
Article
Spectroscopy
Min Huang, Fenghua Geng, Yongxiang Wang, Congying Shao, Gen Liu, Maotian Xu
Summary: The photometric method is widely used in clinical tests due to its simplicity and cost-effectiveness. Novel colorimetric and ratiometric assays are important for accurately quantifying targets, with the challenge lying in designing chromophores with significant spectral shifts before and after binding. The established sensing system has a low limit of detection for ALP and can be applied for detecting ALP in complex clinical samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Fanghui Ma, Qing Zhou, Minghui Yang, Jianglin Zhang, Xiang Chen
Summary: Sulfur quantum dots with a size of approximately 2 nm were synthesized and their fluorescence properties and applications were studied. The fluorescence intensity of the dots could be restored in the presence of alkaline phosphatase, making it a sensitive and selective method for detecting alkaline phosphatase in serum samples.
Article
Cell Biology
Lingmei Jin, Jiangping He, Huijian Feng, Sa Li, He Liu, Hongzhi Dong, MingLi Hu, Junju Huang, Haoyu Wu, Jiekai Chen, Ling Qi, Kaixin Wu
Summary: Deficiency of the histone H3K9 methyltransferase SETDB1 induces RIPK3-dependent necroptosis in mouse embryonic stem cells (mESCs). Reactivation of transposable elements (TEs) upon SETDB1 knockout is responsible for RIPK3 regulation through both cis and trans mechanisms. IAPLTR2_Mm and MMERVK10c-int, suppressed by SETDB1-dependent H3K9me3, act as enhancer-like cis-regulatory elements and their RIPK3 nearby members enhance RIPK3 expression when SETDB1 is knockout. Moreover, reactivated endogenous retroviruses generate excessive viral mimicry, promoting necroptosis mainly through Z-DNA-binding protein 1 (ZBP1). These results indicate the importance of TEs in regulating necroptosis.
CELL DEATH & DISEASE
(2023)
Article
Chemistry, Physical
Zhenyu Ding, Zhe Li, Xiaoxue Zhao, Yanrong Miao, Zhenfeng Yuan, Yuanyuan Jiang, Yizhong Lu
Summary: This study successfully embeds highly dispersed ultrasmall Ru nanoparticles onto carbon nitride (C3N4) nanosheets through a spontaneous redox reaction, forming Ru-C3N4 nanocomposites. The Ru-C3N4 exhibits excellent peroxidase-like activity, which can be regulated by adjusting the loading of Ru nanoparticles. A colorimetric sensing method for alkaline phosphatase (ALP) detection is developed based on the inhibitory effect of ascorbic acid on the color development reaction of Ru-C3N4. The sensor shows a wide linear range and low detection limit for ALP sensing, and satisfactory results are obtained in detecting ALP in human serum.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Cell Biology
Luciano Ferrada, Maria Jose Barahona, Matias Vera, Brent R. Stockwell, Francisco Nualart
Summary: The combination therapy of imidazole ketone erastin (IKE) and oxidized form of vitamin C (DHAA) can restore sensitivity to ferroptosis in previously resistant tumor cells and induce lipid droplet peroxidation, leading to ferroptosis.
CELL DEATH & DISEASE
(2023)
Article
Chemistry, Analytical
Jialin Wen, Yuxuan Hu, Na Li, Dan Li, Guangfan Zheng, Yongjian Zou, Minmin Zhang, Lingling Shui
Summary: In this study, a novel ratiometric fluorescent probe based on in situ fluorogenic reaction was developed for alkaline phosphatase (ALP) activity sensing. The probe showed high sensitivity for ALP activity with a detection limit of 0.08 U/L.
ANALYTICA CHIMICA ACTA
(2022)
Article
Endocrinology & Metabolism
Freja Pontan, Helena Hauta-alus, Saara Valkama, Jenni Rosendahl, Maria Enlund-Cerullo, Sture Andersson, Outi Makitie, Elisa Holmlund-Suila
Summary: This study investigated the concentrations of alkaline phosphatase (ALP) and the prevalence of hyperphosphatasemia in healthy children, and their association with vitamin D, growth, infections, and bone parameters. It was found that boys had higher ALP levels than girls, and hyperphosphatasemia was more common at 12 months. Hyperphosphatasemia was associated with body size, growth rate, and bone mineralization, but not with infections.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Chemistry, Analytical
Xiao-Xue Jiang, Pan Li, Meng-Ya Zhao, Rui-Cong Chen, Zhen-Guang Wang, Jia-Xiu Xie, Yun-Kai Lv
Summary: Sulfur quantum dots (SQDs) were successfully encapsulated into metal-organic frameworks (ZIF-8) using a surface bound zinc ion-induced growth strategy, resulting in SQDs@ZIF-8 for alkaline phosphatase (ALP) detection. This new synthesis procedure significantly improved the luminous efficiency of SQDs. The detection of ALP was achieved by pH-triggered fluorescence quenching of SQDs@ZIF-8, and an extended detection range was obtained by complexing free SQDs with Fe2+. SQDs@ZIF-8 exhibited excellent stability in water and showed potential for fluorescence and colorimetric detection of ALP in human serum.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Kanglin Pei, Di Li, Wenjing Qi, Di Wu
Summary: Fluorescent silver coordination polymer nanoparticles (Ag-TPA CPNs) were synthesized via an ultrarapid microwave-assisted strategy using terephthalic acid (TPA) and silver nitrate. The Ag-TPA CPNs showed high fluorescent quantum yield and large Stokes shift, and were used for the detection of alkaline phosphatase (ALP) activity. The method displayed a linear relationship between fluorescence intensity and ALP concentration, with high selectivity and accuracy in biosample analysis.
Article
Chemistry, Physical
Agustina Reynoso, David Possetto, Eduardo De Geronimo, Virginia C. Aparicio, Jose Natera, Walter Massad
Summary: The kinetic and mechanistic aspects of Riboflavin-sensitized degradation of Triclosan have been studied, showing that the reaction rate between TCS and singlet molecular oxygen is faster under alkaline conditions, and the photosensitized degradation of TCS by Riboflavin is faster than that of phenol.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qi Xiao, Pingping Mu, Gan Ning, Wenqian Zhang, Bo Li, Shan Huang
Summary: Efficient red carbon dots (R-CDs) were synthesized and it was found that polydopamine and L-ascorbic acid affect their fluorescence properties. The R-CDs/polydopamine nano-composite can efficiently detect L-ascorbic acid and alkaline phosphatase in human serum samples.
Article
Toxicology
Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
Summary: This study developed a new method to assess the skin irritation of antimicrobial cleaning products. The method utilized a more human-like model and demonstrated its effectiveness through comparison with in vivo rabbit skin irritation data.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Arupam Patra, Thirukumaran Kandasamy, Siddhartha Sankar Ghosh, Gurvinder Kaur Saini
Summary: This study successfully produced recombinant anisoplin and demonstrated its significant anti-cancer effect and ability to induce apoptosis in breast cancer cells. The activation of related signaling pathways may be the key to cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Summary: This study used live cell imaging to observe the accumulation of amiodarone in primary rat alveolar type II cells, and found that it specifically accumulates in lamellar bodies. The uptake is rapid, while storage is persistent. The main mechanisms for intracellular bioaccumulation of amiodarone are proposed to be passive diffusion, ion-trapping, and lipophilic interactions.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Filiz Bakar-Ates, Erva Ozkan
Summary: This study investigated the ferroptotic effect of CuB in breast cancer cells and evaluated its combination with erastin, a ferroptosis inducer. The results showed that the combination treatment significantly activated the ferroptotic pathways and altered the expression of iron-related proteins in breast cancer cells.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Rachel Z. Bennie, Ian C. Shaw
Summary: Estrogen mimics are synthetic and naturally occurring compounds that can interact with estrogen receptors in animals. In vitro transactivation reporter gene assay and in silico molecular modelling can be used to predict the mimicry of these compounds, reducing reliance on animal studies.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Sevda Isik, Semra Cicek
Summary: This study found that high doses of riboflavin can cause cytotoxicity in rainbow trout gonad cells and affect the transcriptional expressions of antioxidant enzymes and growth and reproductive genes, potentially leading to DNA damage and cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Ying Wang, Jie Wu, Mengqi Wan, Dou Yang, Fasheng Liu, Kehao Li, Manxin Hu, Yuanyuan Tang, Huiqiang Lu, Shouhua Zhang, Yuanzhen Xiong
Summary: m-Cresol is commonly used as an intermediate for pesticides and other industrial applications. This study investigated the hepatotoxicity of m-cresol using zebrafish larvae and explored its molecular mechanisms. The results suggest that m-cresol may induce liver damage in zebrafish larvae through oxidative stress and cell apoptosis pathways.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Vincent J. Manna, Shannon Dwyer, Vanessa Pizutelli, Salvatore J. Caradonna
Summary: The widespread use of electronic cigarettes and the emergence of a new illness have raised concerns about the effects of e-cigarette vapor on respiratory tissues. Researchers have developed a simple device to mimic the response of human airway tissue after long-term exposure to e-cigarette vapor, and have identified differences in the effects of different vapor compositions on airway tissue.
TOXICOLOGY IN VITRO
(2024)
