Article
Multidisciplinary Sciences
Ivan Menendez-Montes, Salim Abdisalaam, Feng Xiao, Nicholas T. Lam, Shibani Mukherjee, Luke I. Szweda, Aroumougame Asaithamby, Hesham A. Sadek
Summary: Mitochondrial utilization of fatty acids induces a significant increase in ROS detection at the chromatin level, indicating that mitochondrial metabolic perturbations directly alter the nuclear redox status, with chromatin being particularly sensitive to the prooxidant effect of FA utilization by the mitochondria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Environmental
Tin Yan Wong, Neng Yan, Kenneth Kin Leung Kwan, Yanrong Pan, Jingjing Liu, Yao Xiao, Long Wu, Henry Lam
Summary: Silver nanoparticles (AgNPs) have raised concerns due to their potential harmful effects on human beings. This study investigated the hepatotoxic effects of AgNPs using a comparative proteomics approach. The results showed that cancerous liver cells and normal cells respond to AgNPs exposure through different pathways, providing new insights into mechanisms underlying AgNPs-induced hepatotoxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
James H. Schofield, Zachary T. Schafer
Summary: The relationship between mitophagy and ROS production is complex and not fully understood. This review discusses mtROS generation and their detrimental effects on cellular viability, along with the cellular defense mechanisms against oxidative stress. Furthermore, the prominent mechanisms governing mitophagy induction that bear on oxidative stress are explored.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Engineering, Environmental
Maria Carbo, Palak Chaturvedi, Ana Alvarez, Daniela Pineda-Cevallos, Arindam Ghatak, Pablo Rodriguez Gonzalez, Maria Jesus Canal, Wolfram Weckwerth, Luis Valledor
Summary: Microplastics, including Bisphenol A (BPA), are major pollutants in aquatic environments. BPA, one of the most abundant and dangerous components, can lead to endocrine disorders and cancer in mammals. This study aimed to understand the molecular-level effects of BPA on plants and microalgae, specifically Chlamydomonas reinhardtii. The findings revealed that BPA disrupts iron and redox homeostasis, leading to cell dysfunction and ferroptosis. Interestingly, the microalgae showed recovery at both molecular and physiological levels, with starch accumulation after 72 hours of BPA exposure. This study provides valuable insights into BPA toxicology, ferroptosis in microalgae, and potential target genes for microplastic bioremediation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Cardiac & Cardiovascular Systems
Jonas Peper, Daniel Kownatzki-Danger, Gunnar Weninger, Fitzwilliam Seibertz, Julius Ryan D. Pronto, Henry Sutanto, David Pacheu-Grau, Robin Hindmarsh, Soeren Brandenburg, Tobias Kohl, Gerd Hasenfuss, Michael Gotthardt, Eva A. Rog-Zielinska, Bernd Wollnik, Peter Rehling, Henning Urlaub, Joerg Wegener, Jordi Heijman, Niels Voigt, Lukas Cyganek, Christof Lenz, Stephan E. Lehnart
Summary: Utilizing proteomic techniques, CAV1 and CAV3 isoform-specific protein interactions were analyzed in cardiomyocytes, revealing McT1 as a metabolically relevant CAV3-specific interactor. CRISPR/Cas9-mediated CAV3 knockout demonstrated the functional stabilization role of McT1 in human cardiomyocytes, leading to action potential prolongation and instability. These findings suggest potential therapeutic targets for muscle-specific assembly function related to lactate metabolism.
CIRCULATION RESEARCH
(2021)
Review
Immunology
Miranda D. Chavez, Hubert M. Tse
Summary: Mitochondrial dysfunction and oxidative stress are associated with T cell-mediated autoimmune diseases, and targeting metabolic pathways can inhibit autoreactive T cell activation. Increasing the requirements of Tregs for ROS and oxidative phosphorylation can promote self-tolerance and inhibit the activity of autoreactive T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrew P. Bischer, Timothy M. Baran, Andrew P. Wojtovich
Summary: Environmental surveillance-mediated behavior in Caenorhabditis elegans integrates multiple cues and is coordinated by neurons through signaling cascades. Despite lacking eyes, C. elegans is able to perceive and react to the color blue. This study explains this color perception by showing that internally-generated reactive oxygen species (ROS), in response to light, add to exogenous sources of ROS. Multiple sub-threshold sources of ROS are integrated to coordinate behavioral responses with internal cues. Blue light affects C. elegans behavior through ROS generation by endogenous flavins, mediated by the neuronal gustatory photoreceptor like protein, LITE-1. Overall, this study demonstrates that ROS and LITE-1 play central roles in C. elegans foraging behavior through integration of multiple inputs, including light.
Article
Immunology
Nan-Nan Liang, Ying Zhao, Yue-Yue Guo, Zhi-Hui Zhang, Lan Gao, De-Xin Yu, De-Xiang Xu, Shen Xu
Summary: This study found that mitochondria-derived reactive oxygen species (ROS) contribute to renal cell ferroptosis during lipopolysaccharide (LPS)-induced acute kidney injury (AKI). Inhibition of ferroptosis and scavenging of mitochondrial ROS with specific inhibitors, ferrostatin-1 and MitoQ, respectively, attenuated renal lipid peroxidation, ferroptosis-characteristic mitochondrial damage, and renal cell death. These findings suggest that mitochondria-targeted antioxidants may be potential therapeutic agents for sepsis-induced AKI.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ola Karmi, Linda Rowland, Skylar D. King, Camila Manrique-Acevedo, Ioav Z. Cabantchik, Rachel Nechushtai, Ron Mittler
Summary: The lack of CISD2 in cardiomyocytes leads to high levels of iron accumulation and iron-related proteins, potentially impacting the aging process in the heart. However, not all aging features are induced by the absence of CISD2.
Article
Biochemistry & Molecular Biology
Hiromu Ito, Hiromi Kurokawa, Hirofumi Matsui
Summary: Mitochondria, essential organelles for energy production in eukaryotes, generate reactive oxygen species during the process which play pivotal roles in cell signaling and iron homeostasis regulation. The regulation of iron transportation, involving proteins like HCP1, DMT1, and mitoferrin, is increasingly understood in relation to mitochondrial reactive oxygen species and diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Andrey Y. Vinokurov, Olga A. Stelmashuk, Polina A. Ukolova, Evgeny A. Zherebtsov, Andrey Y. Abramov
Summary: The brain produces various reactive oxygen species at different rates in different brain regions, with the brain stem and cerebellum being more sensitive to oxidative stress. Mitochondrial ROS has minor implications to total ROS production, and levels of lipid peroxidation and GSH vary across brain regions.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Engineering, Biomedical
Raymond M. Wang, Joshua M. Mesfin, Jervaughn Hunter, Paola Cattaneo, Nuno Guimaraes-Camboa, Rebecca L. Braden, Colin Luo, Ryan C. Hill, Monika Dzieciatkowska, Kirk C. Hansen, Sylvia Evans, Karen L. Christman
Summary: This study demonstrates that myocardial matrix hydrogel exhibits the ability to scavenge reactive oxygen species and protect cells from oxidative stress, as well as enhance cell proliferation. In in vivo experiments using a rat myocardial infarction model, animals treated with matrix injection showed increased thymidine analog uptake in cardiomyocytes, reduced oxidative damage, and improved preservation of tissue free thiol content compared to saline controls. Overall, this study provides evidence that the properties of the myocardial matrix material create a supportive microenvironment for cardiomyocytes undergoing DNA synthesis, potentially facilitating DNA repair or cell cycle activation.
ACTA BIOMATERIALIA
(2022)
Article
Toxicology
Dohee Ahn, Cho-Won Kim, Ryeo-Eun Go, Kyung-Chul Choi
Summary: This study aimed to determine a mitochondrial biomarker that measures the toxicity of mitochondria and propose an efficient evaluation system. The results show that MitoSOXTM red fluorescence dye can be used as a reliable method to measure mitochondrial oxidative toxicity.
TOXICOLOGY IN VITRO
(2022)
Article
Biochemistry & Molecular Biology
Naihui Guo, Shengjia Tang, Jiayu Wang, Shikai Hu, Shaoqing Tang, Xiangjin Wei, Gaoneng Shao, Guiai Jiao, Zhonghua Sheng, Peisong Hu
Summary: This study investigated the mechanism of hormone and reactive oxygen species (ROS) crosstalk in regulating rice dormancy using Zhonghua11 (ZH11) and Introgression line (IL) varieties. Transcriptome and proteome analysis identified differentially expressed genes (DEGs) and proteins (DEPs) between IL and ZH11. Hormone content detection revealed significant differences in abscisic acid (ABA) and jasmonic acid (JA) levels between IL and ZH11. ROS content measurement showed variations in hydrogen peroxide (H2O2) and superoxide anion (O-2(.-)) levels between IL and ZH11. These findings suggest that hormones and ROS play a role in rice dormancy regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Michael O. Isei, Don Stevens, Collins Kamunde
Summary: The research found that heart mitochondria show different responses to copper and thermal stress, and the consumption capacity of H2O2 is influenced by the type of substrate, temperature, and copper. The NADPH-dependent peroxidase system is the centerpiece of ROS scavenging in heart mitochondria, and copper has the ability to inhibit specific mitochondrial ROS scavenging pathways.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Biophysics
Alejandra N. Torres, Ludgero Tavares, Maria J. Pereira, Jan W. Eriksson, John G. Jones
Summary: This study characterized the fatty acid composition and positional distribution of triglycerides in human subcutaneous adipose tissue using C-13 NMR spectroscopy. The study found that the sn2 position of triglycerides is enriched with monounsaturated fatty acids, while the sn1,3 position is enriched with saturated fatty acids. Furthermore, the study revealed a significant positive correlation between palmitoleic acid levels and both BMI and insulin sensitivity.
NMR IN BIOMEDICINE
(2023)
Article
Toxicology
Ana Reis-Mendes, Felix Carvalho, Fernando Remiao, Emilia Sousa, Maria de Lourdes Bastos, Vera Marisa Costa
Summary: This study compares the cardiotoxicity of MTX and its metabolite NAPHT in an in vitro cardiac model and explores the role of metabolism in the cardiotoxic effects. The results show that MTX causes concentration-dependent cytotoxicity, affecting mitochondrial function and lysosome uptake, while NAPHT only exhibits concentration-dependent cytotoxicity. Inhibition or induction of metabolism has minimal impact on MTX-induced cytotoxicity, suggesting that metabolism is not a major contributor to MTX toxicity. However, autophagy is involved in MTX-induced cytotoxicity, potentially through the involvement of p62/LC3-II.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Chemistry, Medicinal
Sofia Benfeito, Carlos Fernandes, Daniel Chavarria, Sandra Barreiro, Fernando Cagide, Lisa Sequeira, Jose Teixeira, Renata Silva, Fernando Remiao, Paulo J. Oliveira, Eugenio Uriarte, Fernanda Borges
Summary: Although TPP+ cation is commonly used to target antioxidants to mitochondria, it has shown cytotoxicity in vitro. This study explored nitrogen-derived cationic carriers as alternative compounds to TPP. The results showed that these nitrogen-based cationic carriers were not cytotoxic and exhibited neuroprotective effects against oxidative insults.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Tania Lima, Joao Eduardo Rodrigues, Bruno Manadas, Rui Henrique, Margarida Fardilha, Rui Vitorino
Summary: This study reanalyzed a urine proteome dataset from prostate cancer patients and controls using a peptide-centric approach. Compared to the conventional protein-centric approach, the peptide analysis revealed more relevant information related to prostate cancer, highlighting the importance of peptide analysis in proteomics research.
JOURNAL OF PROTEOMICS
(2023)
Article
Environmental Sciences
Alexandra Martins, Diana Dias da Silva, Renata Silva, Felix Carvalho, Lucia Guilhermino
Summary: This study investigated the long-term toxicity of lithium (Li) and lithium microplastics (Li-MPs mixtures) under high light intensity and warmer water temperature. The results showed that both high light intensity and warmer temperature increased the toxicity of Li and Li-MPs mixtures, with higher concentrations leading to population extinction.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Catia R. Lopes, Andreia Oliveira, Ingride Gaspar, Matilde S. Rodrigues, Joana Santos, Eszter Szabo, Henrique B. Silva, Aangelo R. Tome, Paula M. Canas, Paula Agostinho, Rui A. Carvalho, Rodrigo A. Cunha, Ana Patricia Simoes, Joao Pedro Lopes, Samira G. Ferreira
Summary: Moderate caffeine intake can antagonize adenosine receptors and protect neurons without affecting behavior and synaptic plasticity. It increases the metabolic competence of synapses and enhances their antioxidant capacity.
Article
Biochemistry & Molecular Biology
Ana Sofia Almeida, Barbara Silva, Joao Pedro Silva, Jose Augusto Pereira, Fernando Remiao, Carla Fernandes
Summary: Synthetic cathinones, such as MDPV, are widely abused for their psychostimulant effects. In this study, the stereochemical stability of MDPV was investigated, and the enantiomers were separated and identified. The racemization of MDPV was found to be affected by temperature. The enantioselectivity of MDPV in cytotoxicity and protein expression was also evaluated, but no enantioselectivity was observed.
Review
Pharmacology & Pharmacy
Ines Costa, Daniel Jose Barbosa, Sofia Benfeito, Vera Silva, Daniel Chavarria, Fernanda Borges, Fernando Remiao, Renata Silva
Summary: Ferroptosis is a regulated cell death process involving the accumulation of iron and reactive oxygen species, as well as inhibition of system Xc-, glutathione depletion, and lipid peroxidation. It is implicated in various brain diseases and has potential therapeutic applications in cancer treatment. Understanding the mechanisms and role of ferroptosis is crucial for developing new treatment strategies and targets.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Oncology
Rui Vitorino, Antonio S. Barros, Sofia Guedes, Douglas C. Caixeta, Robinson Sabino-Silva
Summary: NIR spectroscopy is a valuable tool for cancer diagnosis and treatment. It reveals inherent differences between tumor and normal tissue and provides insights into cancer patterns and physiology. The integration of NIR spectroscopy with advanced algorithms makes it a cutting-edge technology with great potential in medical applications.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Biochemical Research Methods
Maria J. Cardoso Ferreira, Tania Soares Martins, Steven R. Alves, Ilka Martins Rosa, Jonathan Vogelgsang, Niels Hansen, Jens Wiltfang, Odete A. B. da Cruz e Silva, Rui Vitorino, Ana Gabriela Henriques
Summary: Aging is the main risk factor for age-related neurodegenerative diseases, such as Alzheimer's disease (AD), which is characterized by the presence of senile plaques (SPs) and neurofibrillary tangles (NFTs). This study conducted a literature review to identify the proteomes of SPs and NFTs, followed by a bioinformatic analysis and validation of potential biomarkers.
Article
Polymer Science
Ana Isabel Ribeiro, Barbara Vieira, Catia Alves, Barbara Silva, Eugenia Pinto, Fatima Cerqueira, Renata Silva, Fernando Remiao, Vasyl Shvalya, Uros Cvelbar, Jorge Padrao, Alice Maria Dias, Andrea Zille
Summary: This article mainly introduces a new type of halochromic textile that can change color with variations in acidity and alkalinity. The properties and potential applications of this halochromic textile are detailed in the article, which shows promising prospects in various fields such as biomedicine, packaging, filtration, agriculture, protective apparel, sports, camouflage, architecture, and design.
Article
Biochemical Research Methods
Rita Ferreira, Francisco Amado, Rui Vitorino
Summary: Bioinformatics plays a critical role in advancing peptidomics by providing powerful tools for accelerating peptide discovery, understanding their functions, and gaining insights into complex molecular interactions.
Article
Pharmacology & Pharmacy
Vera Marisa Costa, Joao Paulo Capela, Maria Lourdes Bastos, Fernando Remiao, Kurt James Varner, Jose Alberto Duarte, Felix Carvalho
Summary: Prolonged overexposure to catecholamines causes toxicity due to continuous adrenoceptor stimulation, autoxidation, and reactive pro-oxidant species formation. This study investigated the contribution of oxidative stress in adrenaline-induced neurotoxicity using non-differentiated SH-SY5Y cells. Results showed that Adrenaline caused concentration- and time-dependent cytotoxicity in the cells, and N-acetyl-cysteine prevented the toxicity while the antioxidant Tiron was non-protective. Further analysis is needed to understand the involvement of these processes in catecholamine-induced peripheral neuropathy.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Jessica Veiga-Matos, Ana I. Morales, Marta Prieto, Fernando Remiao, Renata Silva
Summary: This comprehensive review explores various models and techniques used to assess drug-induced P-gp modulation, highlighting the therapeutic implications of these modulators. Researchers present different approaches, including in vitro and in vivo experiments, and discuss their strengths and limitations. Additionally, the review focuses on the therapeutic significance of P-gp-mediated renal and intestinal elimination.