Review
Biochemistry & Molecular Biology
Nattaya Damkham, Surapol Issaragrisil, Chanchao Lorthongpanich
Summary: This review provides an update on the regulation of YAP and TAZ, transcriptional coactivators in the Hippo signaling pathway, in stem cell behaviors and differentiation. It highlights their roles in mechanosensing and mechanotransduction, as well as the importance of understanding how cells perceive and respond to their microenvironment for improving the efficiency and efficacy of stem cell products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Toxicology
Aafke W. F. Janssen, Loes P. M. Duivenvoorde, Deborah Rijkers, Rosalie Nijssen, Ad A. C. M. Peijnenburg, Meike van der Zande, Jochem Louisse
Summary: The study evaluated the expression of CYP enzymes and chemical-induced changes in a hiPSC-derived HIO model, showing higher gene expression levels of important intestinal CYP enzymes in HIOs compared to other models. CYP3A4 activity was induced by phenobarbital and rifampicin in HIOs, indicating the potential of hiPSC-based HIOs as a useful in vitro intestinal model for studying the biotransformation of chemicals in the intestine.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Hematology
Haiyun Pei, Huilin Li, Lei Xu, Bowen Zhang, Heng Zhang, Yali Jia, Liqing Liang, Xiaoyan Xie, Zeng Fan, Zhou Yang, Xiaoling Wang, Feiling Song, Lijuan He, Wen Yue, Xuetao Pei
Summary: The major challenge in using human induced pluripotent stem cells (hiPSCs) is their efficient and controlled differentiation into specific cell types. This study focused on understanding the characteristics of hiPSCs derived from human umbilical arterial endothelial cells (HuA-iPSCs) and their differentiation potential. It was found that HuA-iPSCs retained a transcriptional memory and DNA methylation signature similar to their parental cells, and they showed the highest efficiency in differentiating into hematopoietic cells compared to other hiPSCs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Roshina Thapa, Erez Elfassy, Leonid Olender, Omri Sharabi, Roi Gazit
Summary: Adult hematopoietic stem cells (HSCs) in the bone marrow (BM) are quiescent, but can be activated by perturbations such as blood loss or infection. The earliest stages of HSC activation are not well understood. Surface markers CD69 and CD317 show a response as early as 2 h after stimulation, with varying expression patterns depending on the type of immune stimuli.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Chiyuan Ma, Yue Xiong, Pei Han, Xueying Zhang, Yujing Cao, Baobei Wang, Huashan Zhao, Enkui Duan, Jian V. Zhang, Xiaohua Lei
Summary: This study demonstrates for the first time the significant impact of microgravity on hematopoietic differentiation of human pluripotent stem cells (hPSCs), and reveals the underlying mechanism for the effect of simulated microgravity on hematopoiesis development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Yifan Wang, Tengfei Zhao, Yiren Jiao, Hanxu Huang, Yongxiang Zhang, Ao Fang, Xuhua Wang, Yanling Zhou, Haochen Gu, Qionghua Wu, Jiang Chang, Fangcai Li, Kan Xu
Summary: This study investigates the effect of Laponite nanoplatelets on stem cell therapy. The results show that Laponite nanoplatelets can induce the neuronal differentiation of neural stem cells within five days in vitro, and the NF-kappa B pathway is involved in this process. Moreover, Laponite nanoplatelets can increase the survival rate of transplanted neural stem cells and promote their differentiation into mature neurons. The formation of connections between transplanted cells and host cells is confirmed by axon tracing. Therefore, Laponite nanoplatelets can be considered a convenient and practical biomaterial to enhance the efficacy of neural stem cell transplantation and promote repair of the injured spinal cord.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Biochemistry & Molecular Biology
Sajan George, Michael R. Hamblin, Heidi Abrahamse
Summary: Adipose stem cells have the potential to differentiate into neurons, and autologous differentiated stem cells can be used in nerve replacement therapies. Genetically modified immortalized stem cells have the advantage of accessibility and ease of propagation. Photobiomodulation, a therapy using laser or light, can modulate stem cell proliferation and differentiation. This study compared the neuronal differentiation potential of primary and immortalized stem cells using photobiomodulation and found that primary stem cells demonstrated higher differentiation potential.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mehdi Farokhi, Fatemeh Mottaghitalab, Mohammad Reza Saeb, Shahrokh Shojaei, Negin Khaneh Zarrin, Sabu Thomas, Seeram Ramakrishna
Summary: Currently, there are no clinically available methods to enhance neural structural regeneration and functional recovery of nerve injuries. Conductive materials, such as polypyrrole and graphene, have been considered as one of the best choices for nerve regeneration due to their capacity to control the growth and differentiation of neural stem cells.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Oncology
Bo Li, Can Can, Wancheng Liu, Xiaodong Guo, Hanyang Wu, Yihong Wei, Jinting Liu, Xinyu Yang, Wenbo Jia, Daoxin Ma
Summary: This study aimed to investigate the role of hematopoietic stem cells (HSCs) in acute leukemia treatment. The results showed that HSCs and their lytic extracts can inhibit the proliferation of leukemia cells and promote the differentiation of acute myelogenous leukemia (AML) cells, which may have significant implications for the differentiation therapy of leukemia.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Review
Cell Biology
Bai Ling, Yunyang Xu, Siyuan Qian, Ze Xiang, Shihai Xuan, Jian Wu
Summary: Hematopoietic stem cells (HSCs) play a crucial role in the hematopoietic system as they can self-renew and differentiate into various blood cells. The mTOR signaling pathway is an important regulator of HSCs' differentiation, self-renewal, and quiescence, and several molecules can modulate these potentials by influencing this pathway. This review discusses the regulation of HSCs' three potentials by the mTOR signaling pathway and highlights potential regulators. Additionally, the clinical significance of studying this regulation and future predictions are also outlined.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jacqueline Feyen, Zhen Ping, Lanpeng Chen, Claire van Dijk, Tim V. D. van Tienhoven, Paulina M. H. van Strien, Remco M. Hoogenboezem, Michiel J. W. Wevers, Mathijs A. Sanders, Ivo P. Touw, Marc H. G. P. Raaijmakers
Summary: Myeloid cells reduce the function of HSCs through interferon signaling via a neutrophil-NK cell dependent process.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Weiwei Xue, Caixia Fan, Bing Chen, Yannan Zhao, Zhifeng Xiao, Jianwu Dai
Summary: Transplantation of neural stem cells shows promise for restoring communication in spinal cord injury, but the inhibitory microenvironment often leads to glial differentiation rather than neuronal differentiation. Functional biomaterials can mitigate the adverse effects of the SCI microenvironment and promote neuronal differentiation of NSCs.
Article
Biotechnology & Applied Microbiology
Sonali Rawat, Krishan Gopal Jain, Deepika Gupta, Pawan Kumar Raghav, Rituparna Chaudhuri, Pinky, Adeeba Shakeel, Varun Arora, Harshita Sharma, Debika Debnath, Ankarao Kalluri, Ashwini K. Agrawal, Manjeet Jassal, Amit K. Dinda, Prabir Patra, Sujata Mohanty
Summary: The study successfully differentiated mesenchymal stem cells into dopaminergic neurons using PCL-G nanocomposites, showing promising potential. The newly differentiated neurons exhibited unique features, including enhanced neuronal arborization, intracellular Ca2+ influx, and dopamine secretion.
Article
Microbiology
Mitesh Patel, Arif Jamal Siddiqui, Syed Amir Ashraf, Malvi Surti, Amir Mahgoub Awadelkareem, Mejdi Snoussi, Walid Sabri Hamadou, Fevzi Bardakci, Arshad Jamal, Sadaf Jahan, Manojkumar Sachidanandan, Mohd Adnan
Summary: This study investigated the effectiveness of a biosurfactant derived from Lactiplantibacillus plantarum on quorum sensing (QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Chromobacterium violaceum. The biosurfactant showed promising interference with QS and biofilm formation in both bacteria, indicating its potential as an anti-biofilm agent.
Article
Chemistry, Medicinal
Mohd Kamil Hussain, Shaista Ahmed, Andleeb Khan, Arif Jamal Siddiqui, Shahnaaz Khatoon, Sadaf Jahan
Summary: Mucormycosis is a fungal infection that worsens over time if left undiagnosed and untreated. It is specifically associated with the current COVID-19 pandemic. Immuno-compromised patients are particularly vulnerable to both COVID-19 and mucormycosis. Risk factors for this infection include diabetes, corticosteroid medicines, and a weakened immune system. Treatments for mucormycosis are available but less effective, so researchers are focusing on promising agents such as liposomal amphotericin B, manogepix, echino-candins, isavuconazole, and posacanazole.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Arif Jamal Siddiqui, Mitesh Patel, Mohd Adnan, Sadaf Jahan, Juhi Saxena, Mohammed Merae Alshahrani, Abdelmushin Abdelgadir, Fevzi Bardakci, Manojkumar Sachidanandan, Riadh Badraoui, Mejdi Snoussi, Allal Ouhtit
Summary: The objective of this study was to synthesize bacteriocin-derived silver nanoparticles (Bac10307-AgNPs) from Lactobacillus acidophilus (L. acidophilus) to enhance the bioactivity of bacteriocins and overcome their commercial limitations. The synthesized Bac10307-AgNPs showed strong antibacterial activity, promising antioxidant activity, and cytotoxicity against human liver cancer cells.
Article
Biology
Arif Jamal Siddiqui, Vikash Kumar, Sadaf Jahan, Mohammed Merae Alshahrani, Ahmed Abdullah Al Awadh, Maqsood Ahmed Siddiqui, Walid Sabri Hamadou, Abdelmushin Abdelgadir, Juhi Saxena, Riadh Badraoui, Mejdi Snoussi, Mohd Adnan
Summary: Two drugs, C22 and C27, have been found to selectively inhibit human ELOVL1 enzyme, blocking the binding of long-chain fatty acids. This study provides important insights for the discovery and design of novel, selective, and potent ELOVL1 inhibitors.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Arif Jamal Siddiqui, Sadaf Jahan, Mitesh Patel, Abdelmushin Abdelgadir, Wael Alturaiki, Fevzi Bardakci, Manojkumar Sachidanandan, Riadh Badraoui, Mejdi Snoussi, Mohd Adnan
Summary: This study identifies six new inhibitors against the EGFR protein using computational approaches. These molecules show high docking scores and FEC values, as well as stable RMSD plots and strong hydrogen interactions. These findings have potential implications for developing new drugs to treat breast cancer.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biology
Alam Jamal, Mahmood Hassan Dalhat, Sadaf Jahan, Hani Choudhry, Mohammad Imran Khan
Summary: This study evaluated the effect of BTYNB on leukemic cells and found that BTYNB inhibited the function of IGF2BP1, promoting cell differentiation in leukemia.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Review
Immunology
Arif Jamal Siddiqui, Jyoti Bhardwaj, Juhi Saxena, Sadaf Jahan, Mejdi Snoussi, Fevzi Bardakci, Riadh Badraoui, Mohd Adnan
Summary: Malaria and schistosomiasis are two leading causes of morbidity and mortality worldwide. Co-infections are common in the tropics, and clinical consequences are influenced by various factors. While effective drugs exist, genetic mutations can lead to drug resistance, and vaccines are currently lacking. Therefore, highlighting current vaccine candidates and progress is crucial.
Article
Chemistry, Multidisciplinary
Arif Jamal Siddiqui, Sadaf Jahan, Swati Chaturvedi, Maqsood Ahmed Siddiqui, Mohammed Merae Alshahrani, Abdelmushin Abdelgadir, Walid Sabri Hamadou, Juhi Saxena, Bharath K. Sundararaj, Mejdi Snoussi, Riadh Badraoui, Mohd Adnan
Summary: Fatty acids play a crucial role in energy balance regulation in mammals, with both exogenous ingestion and endogenous de novo lipogenesis contributing to their production. Enzymatic elongation of fatty acids beyond 16 carbons can lead to the formation of very long chain fatty acids (VLCFA), regulated by rate-limiting condensation. Among the seven elongases identified in mammals, ELOVL1 has garnered recent interest as a potential therapeutic target for various diseases, including neurological disorders associated with the elongation of specific monounsaturated and saturated acyl-CoAs. ELOVL1 and VLCFAs have direct implications for neurological diseases, and other symptoms such as ichthyotic keratoderma, spasticity, and hypomyelination are also linked to the key enzyme. This review provides unique insights into the therapeutic potential of ELOVL1 and its role in neurological disorders.
Article
Mathematics
Arif Jamal Siddiqui, Sadaf Jahan, Maqsood Ahmed Siddiqui, Andleeb Khan, Mohammed Merae Alshahrani, Riadh Badraoui, Mohd Adnan
Summary: Neurological disorders are characterized by neuron loss and disability. Neurotransmitters are regulated by monoamine oxidase (MAO), and treating disorders like Alzheimer's and Parkinson's involves MAOIs. This study used computational tools to find new MAO-B inhibitors, identifying five molecules with high binding affinity.
Article
Biochemistry & Molecular Biology
Mohammad Imran Khan, Hani Choudhry, Sadaf Jahan, Irfan A. A. Rather
Summary: With the increasing prevalence of multi-drug-resistant fungal infections, especially in developing countries, the development of new strategies and drugs to overcome drug resistance in fungal pathogens is crucial. This study investigated the potential of human neutrophil peptide (HNP) as a treatment for Candida infections. Results showed that HNP alone and in combination with fluconazole (FLC) exhibited antifungal activity against both susceptible and resistant C. albicans isolates. Furthermore, HNP inhibited biofilm formation and the activity of drug efflux pumps and their genes. These findings suggest that HNP could be used as a monotherapy or in combination with FLC to treat azole-resistant C. albicans.
Article
Biology
Ahmed Abdel-Hadi, Danish Iqbal, Raed Alharbi, Sadaf Jahan, Omar Darwish, Bader Alshehri, Saeed Banawas, Manikanadan Palanisamy, Ahmed Ismail, Sahar Aldosari, Mohammed Alsaweed, Yahya Madkhali, Mehnaz Kamal, Faria Fatima
Summary: This study demonstrated the environmentally friendly synthesis of silver nanoparticles using Fusarium oxysporum and their potent antimicrobial activity against various pathogenic microorganisms. The nanoparticles showed significant inhibition zones against a number of bacterial and fungal strains, possibly due to the presence of fungal proteins.
Review
Geriatrics & Gerontology
Apoorv Sharma, Abhishek Kumar Singh
Summary: Aging-induced neurodegenerative diseases (NDs) are a growing health problem worldwide, and oxidative stress is considered a potential cause. Currently, there are no drugs available for the treatment of NDs, so it is necessary to develop strategies/treatments to prevent or cure these age-related diseases. Caloric restriction and intermittent fasting have shown positive effects on healthspan and lifespan, but strict adherence to these routines is difficult, leading to the development of calorie restriction mimetics (CRMs). CRMs are natural compounds that mimic the effects of caloric restriction and activate autophagy. They have been found to regulate redox signaling, enhance antioxidant defense systems, and inhibit ROS generation. Additionally, CRMs also promote neuronal cell survival by regulating redox-sensitive signaling pathways. This article discusses the neuroprotective effects of various CRMs at the molecular and cellular levels during brain aging, and highlights their potential as a cornerstone in the pharmaceutical arsenal against aging and age-related pathologies.
Article
Biochemistry & Molecular Biology
Danish Iqbal, Mohammed Alsaweed, Qazi Mohammad Sajid Jamal, Mohammad Rehan Asad, Syed Mohd Danish Rizvi, Moattar Raza Rizvi, Hind Muteb Albadrani, Munerah Hamed, Sadaf Jahan, Hadeel Alyenbaawi
Summary: Neurodegenerative disorders, such as Alzheimer's disease, have a negative impact on the economy and psychological well-being. This study uses computational screening to identify potential multi-target inhibitors for Alzheimer's disease by analyzing fungal metabolites. The compound Bisacremine-C shows promising activity against GSK-3 beta, the NMDA receptor, and BACE-1, making it a potential candidate for further experimental validation and clinical trials.
Meeting Abstract
Dermatology
M. P. Kashyap, R. Sinha, K. Goliwas, L. Jin, J. Deshane, C. A. Elmets, C. Raman, M. Athar
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Meeting Abstract
Dermatology
C. Raman, M. P. Kashyap, B. Mishra, J. Deshane, S. M. Mukhtar, M. Athar
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
