Article
Multidisciplinary Sciences
Hankyu Lee, Alba E. Marin-Araujo, Fabio G. Aoki, Siba Haykal, Thomas K. Waddell, Cristina H. Amon, David A. Romero, Golnaz Karoubi
Summary: This study utilized computational fluid dynamics to redesign the fluid delivery system of a trachea bioreactor and improve the perfusion cell seeding protocol to promote uniform cell deposition. The research demonstrated that low rates of rotation provide more uniform cell deposition patterns and enhance re-epithelialization of long segment tracheal grafts. Lessons learned from this study can be applied to culturing of any tissue engineered tubular scaffold.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Pasqualina Scala, J. Lovecchio, E. P. Lamparelli, R. Vitolo, V Giudice, E. Giordano, C. Selleri, L. Rehak, N. Maffulli, G. Della Porta
Summary: An in-vitro model of human bone marrow mesenchymal stem cells (hBM-MSCs) myogenic commitment was developed using static co-seeding and perfused culture systems. Static co-seeding showed a significant increase in gene expression, while the myogenic induction ability of hSkMs on hBM-MSCs under perfusion conditions was mainly exerted through paracrine effect. The dynamic culture also resulted in a significant increase in anti-inflammatory cytokine gene expression, confirming the immunomodulatory activity of hBM-MSCs along with commitment events.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Article
Ophthalmology
Susannah Waxman, Alicja Strzalkowska, Chao Wang, Ralitsa Loewen, Yalong Dang, Nils A. Loewen
Summary: Transplanting TM cells into a decellularized, tissue-engineered anterior segment eye culture can restore outflow structure and function. The transplanted cells can localize to the TM and infiltrate the extracellular matrix, similar to normal eyes. The engineered eyes can reestablish a normal intraocular pressure and be used for mimicking glaucomatous pressure elevation.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Cell & Tissue Engineering
Jae Min Cha, Yu-Shik Hwang, Dong-Ku Kang, Jun Lee, Elana S. Cooper, Athanasios Mantalaris
Summary: This study developed a novel bioreactor for mass-production of stem cell-based 3D tissue constructs. The bioreactor successfully maintained optimal culture environments for stem cell proliferation, differentiation, and tissue formation, and demonstrated better outcomes in large bone defects.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Khaled Ramadan, Bruno Gomes, Mauricio Pipkin, Mariola Olkowicz, Barbara Bojko, Arnaud Romeo Mbadjeu Hondjeu, Shaf Keshavjee, Thomas Waddell, Janusz Pawliszyn, Marcelo Cypel
Summary: The study aimed to determine the dose-limiting toxicity of oxaliplatin chemotherapy delivered by in vivo lung perfusion (IVLP) and developed a 72-hour porcine IVLP survival model. Results showed clinically significant lung injury at 80 mg/L oxaliplatin, and mild and subclinical lung injury at 40 mg/L. These findings will guide the initiation of a clinical trial examining IVLP delivery of oxaliplatin.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2021)
Article
Cardiac & Cardiovascular Systems
Robert Qaqish, Yui Watanabe, Konrad Hoetzenecker, Jon Yeung, Manyin Chen, Andrew Pierre, Kazuhiro Yasufuku, Laura Donahoe, Marc de Perrot, Tom Waddell, Shaf Keshavjee, Marcelo Cypel
Summary: Short- and long-term outcomes are similar between recipients of lungs from donation after neurologic determination of death and donation after circulatory determination of death. Different intervals between withdrawal of life-sustaining therapies and cardiac arrest did not impact recipient outcomes, and the maximum acceptable duration of this interval remains to be established.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2021)
Article
Cardiac & Cardiovascular Systems
Biniam Kidane, Sean Higgins, Dhruvin H. Hirpara, Suha Kaaki, Yu Cindy Shen, Frances Allison, Thomas K. Waddell, Gail E. Darling
Summary: Although post-esophagectomy emergency department utilization is high, the majority of visits do not convert to inpatient admission. Anastomotic leak is independently associated with higher odds of conversion to admission. Patients living in regions further away tend to utilize closer local hospitals, resulting in lower conversion rates to admission at the index hospital.
ANNALS OF THORACIC SURGERY
(2021)
Article
Cell & Tissue Engineering
Mohammadali Ahmadipour, Pascal Duchesneau, Daisuke Taniguchi, Thomas K. Waddell, Golnaz Karoubi
Summary: The study showed that cell delivery via negative pressure improved the efficiency and cell surface coverage area of recellularization of bioengineered lungs, indicating that negative pressure cell delivery is a more effective approach.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Multidisciplinary Sciences
Hankyu Lee, Alba E. Marin-Araujo, Fabio G. Aoki, Siba Haykal, Thomas K. Waddell, Cristina H. Amon, David A. Romero, Golnaz Karoubi
Summary: This study utilized computational fluid dynamics to redesign the fluid delivery system of a trachea bioreactor and improve the perfusion cell seeding protocol to promote uniform cell deposition. The research demonstrated that low rates of rotation provide more uniform cell deposition patterns and enhance re-epithelialization of long segment tracheal grafts. Lessons learned from this study can be applied to culturing of any tissue engineered tubular scaffold.
SCIENTIFIC REPORTS
(2021)
Review
Clinical Neurology
Khashayar Hanjani, Mostafa Fatehi, Nis Schmidt, Yayha Aghakhani, Gary J. Redekop
Summary: Over the last 150 years, epilepsy surgery has seen significant advancements, from functional brain maps to the use of EEG and the development of the amobarbital test. Pioneers like Talairach and Bancaud also introduced invasive monitoring of deep brain activity before the era of CT and MRI. Looking ahead, the integration of AI and robotics shows promise for further enhancing surgical outcomes.
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Eric A. Chadwick, Takaya Suzuki, Michael G. George, David A. Romero, Cristina Amon, Thomas K. Waddell, Golnaz Karoubi, Aimy Bazylak
Summary: This work presents a methodology for non-invasive high-spatial resolution 3D X-ray micro-CT of healthy mouse lung vasculature, as well as novel techniques for removing spurious branch artefacts from the skeletonized 3D image. These methods accurately characterize the structure of mouse lung vasculature and aid in studying chronic pulmonary diseases.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Geriatrics & Gerontology
Raul Vicente, Michael Rizzuto, Can Sarica, Kazuaki Yamamoto, Mohammed Sadr, Tarun Khajuria, Mostafa Fatehi, Farzad Moien-Afshari, Charles S. Haw, Rodolfo R. Llinas, Andres M. Lozano, Joseph S. Neimat, Ajmal Zemmar
Summary: This study investigates brain activity after cardiac arrest and during near-death experience (NDE) by analyzing electroencephalography (EEG) recordings from a dying patient. The findings suggest increased gamma activity and decreased power in other frequency bands after cardiac arrest. Interestingly, the left-hemispheric gamma activity is found to be modulated by alpha and theta rhythms, even after cessation of cerebral blood flow.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Review
Rehabilitation
Chloe Haldane, Geoffrey Frost, Emmanuel Ogalo, Sean Bristol, Christopher Doherty, Michael Berger
Summary: The objective of this study was to determine the effect of nerve transfer surgery for brachial plexus injury on patient-reported outcomes. The results showed variable improvement in patient-reported outcomes following the surgery, but there were ongoing limitations and disability.
Article
Materials Science, Biomaterials
Ratna Varma, James Poon, Zhongfa Liao, J. Stewart Aitchison, Thomas K. Waddell, Golnaz Karoubi, Alison P. McGuigan
Summary: The study evaluated the effect of topographical cues on the organization of human tracheal epithelial cells and human induced pluripotent stem cell-derived airway progenitors, finding that cilia alignment in airway cells is influenced by topographical cues under ALI culture conditions.
BIOMATERIALS SCIENCE
(2022)
Article
Clinical Neurology
Saman Arfaie, Pedram Laghaei Farimani, Mohammad Sadegh Mashayekhi, Benyamin Hakak-Zargar, Mostafa Fatehi Hassanabad, Jenna Smith-Forrester, Nicholas Sader, Mojgan Hodaie, Rolando Del Maestro, Sunit Das
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
(2023)
Editorial Material
Clinical Neurology
Mostafa Fatehi, Leeor S. Yefet, Swetha Prakash, Brian D. Toyota, Peter A. Gooderham
Summary: There is considerable variability in the management of LGGs among Canadian neurosurgeons. Most respondents believe that further evaluation of patient selection and treatment strategy advocated by the RTOG9802 trial is needed. There is a trend towards more aggressive surgical resections and the need for more accurate patient risk stratification.
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
(2023)
Letter
Clinical Neurology
Vishwathsen Karthikeyan, Mostafa Fatehi Hassanabad, Brenden Joseph, Peter A. Gooderham
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
(2023)
Letter
Clinical Neurology
Gordon Jewett, Shahin Khayambashi, Geoffrey S. Frost, Benjamin Beland, Angela Lee, Victoria Hodgkinson, Lawrence Korngut, Sameer Chhibber
Article
Medicine, General & Internal
Pedram Laghaei Farimani, Mostafa Fatehi, Bradley M. Chaharyn, Ryojo Akagami
Summary: This case report highlights a rare case of subependymoma in the cerebellopontine angle, emphasizing the importance of surgical intervention and comprehensive research for understanding and treating such rare lesions. This discussion also contributes to the knowledge base regarding the clinical presentation and management strategies for CPA subependymomas.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)