Article
Cell & Tissue Engineering
Siddharth Shanbhag, Salwa Suliman, Samih Mohamed-Ahmed, Carina Kampleitner, Mohamed Nageeb Hassan, Patrick Heimel, Toni Dobsak, Stefan Tangl, Anne Isine Bolstad, Kamal Mustafa
Summary: Three-dimensional spheroid culture can promote osteogenic differentiation of bone marrow mesenchymal stromal cells, while 3D printing allows for customized scaffolds for complex bone defects. This study compared the potential of human BMSC cultured as 2D monolayers or 3D spheroids in constructs for bone regeneration. Results showed a trend for superior in vitro mineralization with 3D BMSC, but similar in vivo performance between 2D and 3D BMSC, indicating PLATMC-HPLG constructs as promising scaffolds for bone tissue engineering.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Siddharth Shanbhag, Carina Kampleitner, Samih Mohamed-Ahmed, Mohammed Ahmad Yassin, Harsh Dongre, Daniela Elena Costea, Stefan Tangl, Andreas Stavropoulos, Anne Isine Bolstad, Salwa Suliman, Kamal Mustafa
Summary: Three-dimensional spheroid culture can enhance osteogenic differentiation and bone regeneration capacity of MSC, showing potential for clinical applications of GPC and BMSC. Combination of HPLG and PLATMC as scaffolds is promising for bone tissue engineering applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
M. Teunissen, F. Verseijden, F. M. Riemers, G. J. V. M. van Osch, M. A. Tryfonidou
Summary: In this study, canine adipose tissue-derived MSCs (cAT-MSCs) were compared with bone marrow-derived MSCs (cBMMSCs) in terms of their in vitro chondrogenic potential with or without bone morphogenetic proteins (BMP). The results showed that cAT-MSCs had lower chondrogenic capacity compared to cBM-MSCs when exposed to BMP-2 or BMP-6, indicating further research is needed to explore their potential for treating osteoarthritis.
VETERINARY JOURNAL
(2021)
Article
Cell & Tissue Engineering
K. Futrega, E. Music, P. G. Robey, S. Gronthos, R. Crawford, S. Saifzadeh, T. J. Klein, M. R. Doran
Summary: The study characterized ovine BMSC and evaluated their ability to repair cartilage defects, concluding that micro-pellets manufactured from ovine articular chondrocytes were more effective than those from ovine BMSC. While ovine BMSC could form cartilage-like tissue in vitro, their effective application in cartilage repair will require successful mitigation of hypertrophy and tissue integration issues.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Yawei Gu, Sebastien Pigeot, Lucas Ahrens, Fabian Tribukait-Riemenschneider, Melika Sarem, Francine Wolf, Andres Garcia-Garcia, Andrea Barbero, Ivan Martin, V. Prasad Shastri
Summary: By developing a bioink system with appropriate rheological properties and using 3D bioprinting technology, it is possible to create high cell density structures of predefined geometry, which can then be implanted into nude mice to generate bone tissue with neovascularization. This method demonstrates the potential for fabricating complex bone tissue for clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Shi-Yong Wang, Ru-Bin Yao, Kai-Shun Yang, Huang-Chien Liang, Chen-Ying Su, Hsu-Wei Fang, Sher-Wei Lim
Summary: The study investigated the potential of vancomycin-loaded Bicera (TM) as a bone substitute to prevent implant-associated osteomyelitis and postoperative infection. Results showed that vancomycin-loaded Bicera (TM) effectively inhibited the growth of MRSA and facilitated new bone growth in bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Franciska Oberdiek, Carlos Ivan Vargas, Patrick Rider, Milijana Batinic, Oliver Goerke, Milena Radenkovic, Stevo Najman, Jose Manuel Baena, Ole Jung, Mike Barbeck
Summary: The study evaluated a composite material of PCL and BCP fabricated via fused deposition modeling in terms of its ex vivo and in vivo properties. Results showed no significant differences in mechanical properties between PCL and PCL + BCP, with good biocompatibility observed for both scaffolds. Additionally, all materials demonstrated osteoconductive properties, with the BCP group showing the highest new bone formation in the calvaria model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Shikha Chawla, Giovanna Desando, Elena Gabusi, Aarushi Sharma, Diego Trucco, Juhi Chakraborty, Cristina Manferdini, Mauro Petretta, Gina Lisignoli, Sourabh Ghosh
Summary: One major limitation of 3D bioprinting is the lack of understanding of the role of bioink in modulating molecular signaling pathways. The interaction between SF-G bioink and hMSCs enhanced several chondrogenic pathways, providing potential for further research in this area. This study represents the first detailed proteomics analysis to identify articular cartilage-specific pathways in SF-G-based 3D bioprinted constructs.
JOURNAL OF MATERIALS RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Hannah K. K. Heywood, Stephen D. D. Thorpe, Renos M. M. Jeropoulos, Paul W. W. Caton, David A. A. Lee
Summary: This study explores the role of sirtuins in the regenerative potential of articular chondrocytes. Sirtuins play a vital role in regulating cartilage homeostasis and can promote the expression of key matrix components while inhibiting pro-inflammatory pathways. Modulating sirtuin activity during the expansion phase of chondrocytes can significantly impact cartilage regeneration. Additionally, modulation of sirtuin activity during monolayer expansion can influence heterochromatin marks and have unexpected effects on matrix synthesis in 3D culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
George W. Fryhofer, Hannah M. Zlotnick, Brendan D. Stoeckl, Megan J. Farrell, David R. Steinberg, Robert L. Mauck
Summary: The study focused on developing a rapid fabrication method for patient-specific engineered cartilage tissues by coupling mesenchymal stromal cell-laden hydrogel with porous polycaprolactone bone integrating phase. Results showed that the fabrication process improved cell viability, biochemical content, and mechanical properties over time, with compressive properties peaking at 5 weeks and interfacial shear properties continuing to improve beyond this point. These methods were also used to create an anatomic implantable cell-seeded biologic joint surface for potential whole joint resurfacing in large animal models.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Cell & Tissue Engineering
Wendy E. Brown, Brian J. Huang, Jerry C. Hu, Kyriacos A. Athanasiou
Summary: This study developed a large, anatomically shaped osteochondral construct with robust mechanical properties for repairing cartilage lesions, showing that early assembly led to significantly improved interfacial properties compared to late assembly.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell Biology
Runfeng Cao, Anqi Zhan, Zheng Ci, Cheng Wang, Yunlang She, Yong Xu, Kaiyan Xiao, Huitang Xia, Li Shen, Depeng Meng, Chang Chen
Summary: The study developed a biomimetic biphasic scaffold for osteochondral defect repair, controlling the composition, stiffness, and pore size to promote adhesion, proliferation, chondrogenic, and osteogenic differentiation of bone marrow stem cells. In vitro and in vivo results showed that the biphasic scaffold significantly enhanced concomitant repair of superficial cartilage and subchondral bone, demonstrating its promising osteochondral repair effect.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Mansoor Chaaban, Adrien Moya, Andres Garcia-Garcia, Robert Paillaud, Romain Schaller, Thibaut Klein, Laura Power, Katarzyna Buczak, Alexander Schmidt, Elisabeth Kappos, Tarek Ismail, Dirk J. Schaefer, Ivan Martin, Arnaud Scherberich
Summary: This study investigates the effects of cell expansion and chondrogenic maturation degree on the process of endochondral ossification (ECO). The results show that the maturation degree of adipose-derived stromal cells (ASC) is closely related to their ability to form bone in vivo, with ECO achieved only for HCTs of intermediate maturation. Meanwhile, the expansion of ASC also results in the enrichment of the stromal fraction, and inducing quiescence can rescue their chondrogenic potential.
Article
Cell & Tissue Engineering
Cansu Gorgun, Maria Elisabetta Federica Palama, Daniele Reverberi, Maria Cristina Gagliani, Katia Cortese, Roberta Tasso, Chiara Gentili
Summary: The secretome of mesenchymal stromal cells (MSCs) derived from different tissue sources is considered an innovative therapeutic tool for regenerative medicine. The study compared middle- and small-sized extracellular vesicles (EVs) released by adipose tissue-derived MSCs (ADSCs) and bone marrow-derived MSCs (BMSCs), showing different protein content and functional effects, with ADSC-EVs overexpressing pro-angiogenic factors and BMSC-EVs containing a higher amount of pro-differentiation and chemotactic proteins. This study highlights the importance of selecting the appropriate cell source of EVs for targeted therapeutic applications.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Engineering, Biomedical
Yujie Hua, Yingying Huo, Baoshuai Bai, Junxiang Hao, Guanhuai Hu, Zheng Ci, Xiaodi Wu, Mengyuan Yu, Xin Wang, Hong Chen, Wenjie Ren, Yixin Zhang, Xiaoyun Wang, Guangdong Zhou
Summary: This study reports a novel strategy for the preparation of biomimetic cartilage-bone integrated scaffolds based on photo-crosslinkable cartilage/bone-derived dECM hydrogels, which are able to reconstruct biphasic cartilage-bone biomimetic microenvironment. The scaffolds provide a 3D microenvironment for osteochondral regeneration and efficiently regulate chondrogenesis of bone marrow mesenchymal stem cells (BMSCs).
MATERIALS TODAY BIO
(2022)
Review
Oncology
Xiaomei Yao, Michelle Ghert, Brendan C. Dickson, Snezana Popovic, Bibianna M. Purgina, Shailendra Verma, Joel Werier, Rita A. Kandel
CANCER TREATMENT REVIEWS
(2020)
Article
Orthopedics
Candace Flynn, Mark Hurtig, Alex zur Linden
Summary: This study investigated the feasibility of using CE-mu CT to evaluate the physical and biochemical properties of cartilage in species with thin cartilage. The results showed that disruption of the superficial zone contributes to an increase in apparent density, which is negatively correlated with the concentration of sulfated glycosaminoglycan (sGAG).
Review
Cell Biology
Lisa M. Julian, William L. Stanford
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell & Tissue Engineering
Vanessa J. Bianchi, Michael Parsons, David Backstein, Rita A. Kandel
Summary: The study shows that the severity of OA does not affect the ability of human chondrocytes to form cartilaginous tissue, but the level of ENG co-receptor in the presence of ALK5 does influence this capability. These findings have important clinical implications for cartilage repair therapies and suggest ENG levels as potential biomarkers for identifying cells that can produce cartilage.
TISSUE ENGINEERING PART A
(2021)
Article
Orthopedics
Rahul Gawri, Ryszard Bielecki, Eric W. Salter, Alena Zelinka, Toshikazu Shiba, Graham Collingridge, Andras Nagy, Rita A. Kandel
Summary: Inorganic polyphosphates (polyP) require calcium influx to regulate chondrocyte matrix production, likely through activating calcium signaling pathway. These findings help in the clinical translation of polyP into a therapeutic agent for cartilage repair.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Geriatrics & Gerontology
Felipe C. K. Duarte, Mark Hurtig, Andrea Clark, Stephen Brown, Jeremy Simpson, John Srbely
Summary: The study found a potential causal association between experimentally-induced spine osteoarthritis and neurogenic inflammatory responses in muscle diseases, suggesting that inflammation caused by spine osteoarthritis may lead to central sensitization and sensory changes.
EXPERIMENTAL GERONTOLOGY
(2021)
Article
Automation & Control Systems
Esmat Sheydaeian, Osezua Obehi Ibhadode, Eugene Hu, Robert Pilliar, Rita Kandel, Ehsan Toyserkani
Summary: This study successfully demonstrates a novel approach for preparing implants for small digit joint regeneration.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2021)
Article
Cell & Tissue Engineering
Frank A. Petrigliano, Nancy Q. Liu, Siyoung Lee, Jade Tassey, Arijita Sarkar, Yucheng Lin, Liangliang Li, Yifan Yu, Dawei Geng, Jiankang Zhang, Ruzanna Shkhyan, Jacob Bogdanov, Ben Van Handel, Gabriel B. Ferguson, Youngjoo Lee, Svenja Hinderer, Kuo-Chang Tseng, Aaron Kavanaugh, J. Gage Crump, April D. Pyle, Katja Schenke-Layland, Fabrizio Billi, Liming Wang, Jay Lieberman, Mark Hurtig, Denis Evseenko
Summary: Osteoarthritis affects millions worldwide, with injuries to the articular surface being a major risk factor. Current cartilage repair strategies are only moderately effective, but a newly developed stem cell-derived formulation shows potential for long-term functional repair.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell Biology
Adam Pietrobon, Julien Yockell-Lelievre, Trevor A. Flood, William L. Stanford
Summary: This study utilizes a genetically engineered human renal organoid model to recapitulate various features of tuberous sclerosis complex (TSC) kidney disease. Single-cell RNA sequencing analysis reveals that the loss of TSC1 or TSC2 affects multiple developmental processes in the renal epithelial, stromal, and glial compartments, including upregulation of stromal-associated genes, rapamycin-insensitive epithelial-to-mesenchymal transition, and the formation of a melanocytic population.
Article
Multidisciplinary Sciences
Mehdi Benlarbi, Genevieve Laroche, Corby Fink, Kathy Fu, Rory P. Mulloy, Alexandra Phan, Ardeshir Ariana, Corina M. Stewart, Jeremie Prevost, Guillaume Beaudoin-Bussieres, Redaet Daniel, Yuxia Bo, Omar El Ferri, Julien Yockell-Lelievre, William L. Stanford, Patrick M. Giguere, Samira Mubareka, Andres Finzi, Gregory A. Dekaban, Jimmy D. Dikeakos, Marceline Cote
Summary: The spike glycoprotein of SARS-CoV-2 can bind to ACE2 through different pathways and mediate membrane fusion. MMP-2 and MMP-9 play important roles in viral entry and infection.
Review
Urology & Nephrology
Adam Pietrobon, William L. L. Stanford
Summary: This review explores the question of how TSC kidney lesions arise, integrating findings from clinical studies, human specimens, and genetic animal models. It presents a new model of pathogenesis suggesting that lesions arise from second-hit events during development. This model can serve as a conceptual framework for investigating TSC lesion pathogenesis in various tissues.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Biochemical Research Methods
Eric Lian, Adam Pietrbon, William L. Stanford
Summary: This article presents a protocol for maintaining and differentiating human pluripotent stem cells into renal organoids. The protocol involves the use of readily made differentiation media, multiplexed sample single-cell RNA-seq analysis, quality control, and immunofluorescence validation of the organoids. It provides a rapid and reproducible model for studying human kidney development and renal diseases. Additionally, it details the use of CRISPR-Cas9 homology-directed repair for genome engineering in the generation of renal disease models.
Meeting Abstract
Orthopedics
A. A. Zelinka, R. A. Kandel
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Orthopedics
Xiangjiang Wang, Rahul Gawri, Changbin Lei, Joon Lee, Gwendolyn Sowa, Rita Kandel, Nam Vo
Summary: The study demonstrates that polyP can enhance synthesis of important substances and increase ATP production in intervertebral disc cells. These findings suggest that polyP may serve as a crucial energy source for disc cells.
Article
Orthopedics
Jasmine E. Chong, J. Paul Santerre, Rita A. Kandel