Article
Biotechnology & Applied Microbiology
Axel J. Delakowski, Jared D. Posselt, Christopher T. Wagner
Summary: This study evaluates the use of an acellular 3D extracellular matrix (ECM) scaffold for tendon/ligament tissue engineering. The study also designs and verifies a novel bioreactor system to support long-term culture with customizable mechanical strain regimens. The results show unique gene regulation patterns and highlight the need for a robust bioreactor system that minimizes process variation.
BIOENGINEERING-BASEL
(2022)
Review
Cell & Tissue Engineering
Wangwang Luo, Yang Wang, Qing Han, Zhonghan Wang, Jianhang Jiao, Xuqiang Gong, Yang Liu, Aobo Zhang, Han Zhang, Hao Chen, Jincheng Wang, Minfei Wu
Summary: This review discusses recent advances in enthesis tissue engineering, particularly the use of biological scaffolds, and provides perspectives on future directions in this field.
JOURNAL OF TISSUE ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Shabnam Anjum, Ting Li, Mohammad Saeed, Qiang Ao
Summary: Tissue engineering has emerged as a primary research topic for treating diseased or damaged tendon/ligament tissue, and decellularized extracellular matrix (dECM) scaffolds show promise for restoring tissue function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Engineering, Biomedical
Chiara Rinoldi, Ewa Kijenska-Gawronska, Ali Khademhosseini, Ali Tamayol, Wojciech Swieszkowski
Summary: Injuries to tendons and ligaments are common and have a significant impact on daily life. Current treatment strategies are sometimes inadequate, leading to the development of tissue engineering and regenerative medicine approaches aiming to stimulate regeneration. The design of artificial scaffolds with tailored mechanical properties is crucial for restoring functionality in such cases.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Darshan Tagadur Govindaraju, Chih-Hao Chen, K. T. Shalumon, Hao-Hsi Kao, Jyh-Ping Chen
Summary: The limited natural healing capacity of tendons and ligaments presents a challenge for effective therapeutic strategies. Tissue engineering, using biomaterials, cells, and biochemical signals, has shown promising results in restoring the physiological functions of these tissues. This paper reviews the structure and healing mechanisms of tendons and ligaments, and discusses the use of bioactive nanostructured scaffolds, with a focus on electrospun fibrous scaffolds, in tissue engineering.
Article
Chemistry, Multidisciplinary
Frederik Schlottmann, Sarah Strauss, Christian Plaass, Bastian Welke, Peter M. Vogt, Joern W. Kuhbier
Summary: The study focused on using spider silk as a carrier matrix to construct tendon-like tissue with high cell vitality and morphology comparable to tenocytes. Although promising, further research is needed to validate its potential in patient-specific tendon reconstruction.
APPLIED SCIENCES-BASEL
(2021)
Review
Engineering, Biomedical
Tingyun Lei, Tao Zhang, Wei Ju, Xiao Chen, Boon Chin Heng, Weiliang Shen, Zi Yin
Summary: This review discusses the challenges and strategies for tendon/ligament-to-bone healing, including biomimetic materials, growth factors, and stem cells. The importance of structure-function relationship, growth factor concentration, and stem cell application in the healing process is highlighted. Unmet needs and emerging strategies in tendon/ligament-to-bone regeneration are also discussed.
BIOACTIVE MATERIALS
(2021)
Review
Engineering, Biomedical
Yexin Li, Ting Deng, Dilihumaer Aili, Yang Chen, Weihong Zhu, Qian Liu
Summary: This review summarizes the application of cell sheet technology in the treatment of tendon and ligament injuries, focusing on the effects of stem cell-derived sheets in mitigating inflammation and promoting healing. It also discusses the prerequisites for future clinical translation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Review
Cell & Tissue Engineering
David L. Butler
Summary: This paper reviews the history of research leading up to the introduction of functional tissue engineering (FTE) for tendon and ligament repair, highlighting the importance of collaborations among surgeons, biologists, and engineers.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Chih-Hao Chen, Dai-Ling Li, Andy Deng-Chi Chuang, Banendu Sunder Dash, Jyh-Ping Chen
Summary: The study prepared core-sheath nanofibers with different structures for tendon tissue engineering, and demonstrated that the combined effects of aligned fibers and PRP could enhance cell proliferation rates and gene expression in tenocytes. Additionally, dynamic cell culture in a bioreactor with cyclic tension stimulation could accelerate tendon maturation in vitro, leading to improved cell proliferation rates and maintenance of tenogenic phenotype compared to static culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Riccardo Gottardi, Kim Moeller, Roberto Di Gesu, Rocky S. Tuan, Martijn van Griensven, Elizabeth R. Balmayor
Summary: The study utilized hyperelastic biphasic 3D printed PLGA scaffolds to mimic the native insertion side, and cultured constructs in dual fluidic bioreactors to achieve regional differentiation towards tenogenic or chondrogenic fate. This system has been validated as a potential platform to form the tendon enthesis and will be further optimized for the fabrication of distinctly biphasic constructs in future studies.
FRONTIERS IN MATERIALS
(2021)
Article
Engineering, Biomedical
Jennifer L. Puetzer, Tianchi Ma, Ignacio Sallent, Amy Gelmi, Molly M. Stevens
Summary: The study successfully demonstrated the development of hierarchical collagen fibers in musculoskeletal tissues using restrained cell-seeded high density collagen gels. The fibers produced were similar to native tissues and showed significant improvement in tensile properties over time. This system holds promise for better understanding cellular regulation of fiber formation and stimulating tissue regeneration after injury.
Article
Biotechnology & Applied Microbiology
Margherita Montorsi, Giada G. Genchi, Daniele De Pasquale, Giorgio De Simoni, Edoardo Sinibaldi, Gianni Ciofani
Summary: Traditional bone tissue engineering has used various types of bioreactors to study osteogenic differentiation, but single-mode bioreactors often fail to mimic physiological conditions effectively. This study developed a multi-modal perfusion bioreactor that induced differentiation of SaOS-2 bone-derived cells and achieved a faster bone matrix deposition rate.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Engineering, Biomedical
Diana Lim, Eric S. Renteria, Drake S. Sime, Young Min Ju, Ji Hyun Kim, Tracy Criswell, Thomas D. Shupe, Anthony Atala, Frank C. Marini, Metin N. Gurcan, Shay Soker, Joshua Hunsberger, James J. Yoo
Summary: Regenerative medicine and tissue engineering provide new therapeutic options for restoring, maintaining, or improving tissue function. To optimize the biological function of tissue-engineered clinical products, specific conditions must be maintained in a bioreactor to allow product maturation and mimic the in vivo environment. Real-time monitoring of product functional capacity is critical for quality management during manufacturing.
BIO-DESIGN AND MANUFACTURING
(2022)
Review
Engineering, Biomedical
Mine Altunbek, Ferdows Afghah, Ozum Sehnaz Caliskan, James J. Yoo, Bahattin Koc
Summary: Tissue interfaces are formed by transitioning of biochemical and mechanical properties in micro-scale, allowing communication and functioning of adjacent tissues. Conventional tissue engineering methods struggle to restore the function of these complex structures, but 3D bioprinting technology provides a precise approach to mimic natural tissue interfaces. This paper reviews and highlights the design of various tissue interfaces using 3D bioprinting, including cartilage-bone, muscle-tendon, tendon/ligament-bone, skin, and neuro-vascular/muscular interfaces. Future directions and translational challenges are also discussed.
Article
Medicine, Research & Experimental
Tianyu Xie, Wenyu Feng, Mingwei He, Xinli Zhan, Shijie Liao, Juliang He, Zhaojie Qin, Feicui Li, Jiake Xu, Yun Liu, Qingjun Wei
Summary: Osteosarcoma is a common malignant bone tumor in children and adolescents, and the five-year survival rate is estimated to be around 70%. This study found that high expression of EVI2B gene is associated with good prognosis in osteosarcoma patients.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Zheng Wang, Chi Ma, Diane Chen, Caitlin Haslett, Chunmei Xu, Changchun Dong, Xiaofang Wang, Minghao Zheng, Yan Jing, Jian Q. Feng
Summary: This study reveals that tendon cells directly form fibrocartilage through cell transdifferentiation, shedding light on the mechanisms underlying enthesopathy and sporting injuries.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Minhao Gao, Bin Zhu, Jing Fan, Youshui Gao, Feng Xue, Guangyi Li, Alysia Hubbard, Xiangrong Gao, Jing Sun, Jing Ling, Longxiang Cao, Delin Liu, Jun Yuan, Qing Jiang, John Papadimitriou, Weiguo Zou, Jian Q. Feng, Liu Yang, Changqing Zhang, Junjie Gao, Minghao Zheng
Summary: In this study, the morphological and transcriptomic expression of osteocytes from murine calvaria and mid-shaft femoral cortical bone were characterized using confocal structured illumination microscopy and mRNA-sequencing analysis. The results showed that calvarial and cortical osteocytes exhibited differences in morphology and gene expression, potentially due to their distinct pathways of ossification.
Article
Medicine, Research & Experimental
Fan Yang, Yuangang Su, Jiamin Liang, Keyi Wang, Haoyu Lian, Junchun Chen, Jiake Xu, Jinmin Zhao, Qian Liu
Summary: Postmenopausal osteoporosis is a chronic metabolic disease characterized by low bone mineral density and bone fragility. In this study, it was found that Casticin inhibits osteoclast activation and differentiation induced by the receptor activator of nuclear factor-κB ligand. Casticin also prevents bone loss and reduces osteoclast activity in vivo, suggesting its potential as a preventive treatment for osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2023)
Article
Biophysics
Trevor N. Savage, David J. Saxby, David G. Lloyd, Claudio Pizzolato
Summary: The purpose of this study was to determine whether calibration of an NMS model resulted in consistent joint contact forces, regardless of EMD value or personalisation of Fo. Hip, knee, and ankle contact forces were predicted for 28 participants using EMG-informed NMS models. The results showed that calibrated models with EMD set between 35 and 70 ms produced similar magnitude and timing of peak joint contact forces.
JOURNAL OF BIOMECHANICS
(2023)
Article
Cell Biology
Shangfu Li, Dian Teguh, Depeng Wu, Lesong Liu, Chaofeng Hu, Jinbo Yuan, Charles A. Inderjeeth, Jiake Xu
Summary: This study investigated the effects of acetylcholinesterase inhibitors (AChEIs) on osteoclast differentiation and function. The findings showed that AChEIs, particularly Donepezil and Rivastigmine, can inhibit osteoclastogenesis and bone resorption by regulating MAPK and NFATc1 signaling pathways through downregulating AChE. These results have important clinical implications for elderly patients with dementia and osteoporosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Review
Cell Biology
Emel Rothzerg, Wendy N. Erber, Christopher L. M. H. Gibbons, David Wood, Jiake Xu
Summary: Osteohematology is an emerging field studying the interaction between hematopoietic and bone stromal cells to understand hematological and skeletal malignancies and diseases. The Notch signaling pathway plays critical roles in embryonic development and cancer progression, including osteosarcoma, leukemia, and multiple myeloma. Dysregulation of Notch-mediated malignant cells disrupts normal bone and bone marrow function, leading to disorders such as osteoporosis and bone marrow dysfunction. This mini-review summarizes the interplay between cells in bone and bone marrow under the Notch signaling pathway in both physiological and tumor microenvironments.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Materials Science, Biomaterials
M. Natividad Gomez-Cerezo, Nataliya Perevoshchikova, Rui Ruan, Kevin M. Moerman, Randy Bindra, David G. Lloyd, Ming Hao Zheng, David J. Saxby, Cedryck Vaquette
Summary: This study proposes the use of a Bone-Ligament-Bone (BLB) 3D-printed polyethylene terephthalate (PET) scaffold for the treatment of ruptured scapholunate interosseous ligament (SLIL). The scaffold mimics the native tissue architecture and exhibits adequate mechanical properties to support physiological loading. Biofunctionalization of the scaffold enhances cell viability and protein secretion, promoting SLIL regeneration.
BIOMATERIALS ADVANCES
(2023)
Article
Multidisciplinary Sciences
Pei Ying Ng, Amy B. P. Ribet, Qiang Guo, Benjamin H. Mullin, Jamie W. Y. Tan, Euphemie Landao-Bassonga, Sebastien Stephens, Kai Chen, Jinbo Yuan, Laila Abudulai, Maike Bollen, Edward T. T. T. Nguyen, Jasreen Kular, John M. Papadimitriou, Kent Soe, Rohan D. Teasdale, Jiake Xu, Robert G. Parton, Hiroshi Takayanagi, Nathan J. Pavlos
Summary: Using organelle-resolution proteomics, the authors identified Slc37a2 as a sugar transporter in osteoclast secretory lysosomes. They found that Slc37a2 is required for the maintenance of skeletal bone mass and that its deficiency leads to high bone mass in mice. This study provides new insights into the molecular composition and spatiotemporal organization of secretory lysosomes in osteoclasts.
NATURE COMMUNICATIONS
(2023)
Review
Pharmacology & Pharmacy
Sipin Zhu, Ping Sun, Samuel Bennett, Oscar Charlesworth, Renxiang Tan, Xing Peng, Qiang Gu, Omar Kujan, Jiake Xu
Summary: This review summarizes the recent research progress of Parthenolide (PTL) as a medicinal compound, including its therapeutic effects on skeletal diseases, cancers, and inflammation-induced cytokine storm. Mechanistic investigations reveal that PTL predominantly inhibits NF-kappa B activation and other signaling pathways. It is also proposed that PTL could inhibit cytokine storms triggered by COVID-19 via blocking the activation of NF-kappa B signaling. Understanding the pharmacologic properties of PTL will assist in developing its therapeutic application for medical conditions such as arthritis, osteolysis, periodontal disease, cancers, and COVID-19-related disease.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Sarah Rashid, Scott G. Wilson, Kun Zhu, John P. Walsh, Jiake Xu, Benjamin H. Mullin
Summary: This study analyzed the transcriptome of human peripheral blood mononuclear cells and their differentiated osteoclast-like cells in culture, identifying differentially expressed genes and associated biological pathways in the progression of osteoporosis. Several well-established osteoclast genes were significantly upregulated in osteoporosis, and the upregulated genes were involved in cell division, migration, adhesion, as well as oxidative phosphorylation, glycolysis, lysosome, and focal adhesion pathways.
Article
Engineering, Biomedical
Kirsten Veerkamp, Christopher P. Carty, Niels F. J. Waterval, Thomas Geijtenbeek, Annemieke I. Buizer, David G. Lloyd, Jaap Harlaar, Marjolein M. van der Krogt
Summary: Spasticity contributes to gait deviations in children with neuromusculoskeletal disorders. This study used predictive simulations to evaluate the pathophysiological mechanisms underlying these deviations. The analysis extracted distinct gait patterns, and the simulation framework predicted gait with velocity- or force-based hyperreflexia. The study found that velocity-based hyperreflexia from specific muscles contributes to different spastic gait patterns, providing insights for targeted treatment.
JOURNAL OF APPLIED BIOMECHANICS
(2023)
Review
Orthopedics
Mengyuan Li, Duanyong Chen, Yuanchen Ma, Minghao Zheng, Qiujian Zheng
Summary: In this study, a systematic review and meta-analysis were conducted to evaluate the effectiveness of stem cell therapy combined with core decompression for avascular necrosis of the femoral head. The results showed that adding stem cell therapy to core decompression improved clinical outcomes.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Engineering, Biomedical
Behzad Shiroud Heidari, Emma Muinos Lopez, Emma Harrington, Rui Ruan, Peilin Chen, Seyed Mohammad Davachi, Benjamin Allardyce, Rangam Rajkhowa, Rodney Dilley, Froilan Granero-Molto, Elena M. De-Juan-Pardo, Minghao Zheng, Barry Doyle
Summary: The addition of 5% silk increases the miscibility between PDO and LCL phases, as well as the surface roughness and hydrophilicity. In vitro experiments show that the silk improves the attachment and proliferation of tendon-derived stem cells, while in vivo studies indicate that it can reduce the expression of pro-inflammatory cytokines. A promising biocomposite was selected and a prototype TL graft was created using extruded fibers, which show suitable tensile properties for ACL repair applications.
BIOACTIVE MATERIALS
(2023)
Article
Geriatrics & Gerontology
Jun Yuan, Steve Pedrini, Rohith Thota, James Doecke, Pratishtha Chatterjee, Hamid R. Sohrabi, Charlotte E. Teunissen, Inge M. W. Verberk, Erik Stoops, Hugo Vanderstichele, Bruno P. Meloni, Christopher Mitchell, Stephanie Rainey-Smith, Kathryn Goozee, Andrew Chi Pang Tai, Nicholas Ashton, Henrik Zetterberg, Kaj Blennow, Junjie Gao, Delin Liu, Frank Mastaglia, Charles Inderjeeth, Minghao Zheng, Ralph N. Martins
Summary: This study investigated the association between plasma SOST levels and brain amyloid-beta load in older individuals. The results showed that plasma SOST levels were higher in individuals with brain amyloid-beta load, and the combination of plasma SOST and Aβ 42/Aβ40 ratio improved the predictive accuracy for preclinical Alzheimer's disease.
