Review
Biotechnology & Applied Microbiology
Chunyan Duan, Mingjia Yu, Changji Hu, Hongying Xia, Ranjith Kumar Kankala
Summary: Tissue engineering aims to restore tissue defects using 3D biomimetic pre-formed scaffolds. Micro-sized injectable modularized units have gained interest as ideal delivery vehicles for cells and growth factors due to their minimally-invasive nature and improved cell retention efficacy. This review discusses the progress of these carriers in tissue engineering, highlighting their design and ability to deliver cells and promote tissue growth.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Lingjiao Zou, Weiqing Zhou, Xiangming Na, Naoki Asakawa, Hua Yue, Guanghui Ma
Summary: 3D cell carriers with porous structures have the potential to investigate drug treatments for tumors. The fabrication of gigantic highly-porous microcarriers allows for high-efficiency 3D cell culture and the construction of artificial tumor tissue models.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Biophysics
Elham Mohseni-Vadeghani, Reza Karimi-Soflou, Sajedeh Khorshidi, Akbar Karkhaneh
Summary: In this study, researchers successfully prepared PLLA microspheres loaded with oxygen-generating biomaterials, preventing hypoxia and protecting cells from oxidative damage. Modified microsphere structures and catalase treatment stably released oxygen and calcium ions, preventing cytotoxicity, making it a promising injectable cell carrier system for bone tissue engineering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Shuntaro Yamada, Mohammed A. A. Yassin, Thomas Schwarz, Kamal Mustafa, Jan Hansmann
Summary: This study investigated the optimal experimental conditions for a perfusion bioreactor system and emphasized the importance of experimental variable optimization for bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Sheng-Long Ding, Xin Liu, Xi-Yuan Zhao, Ke-Tao Wang, Wei Xiong, Zi-Li Gao, Cheng-Yi Sun, Min-Xuan Jia, Cheng Li, Qi Gu, Ming-Zhu Zhang
Summary: The successful regeneration of cartilage tissue at a clinical scale has been a significant challenge. Microcarrier systems offer an attractive approach to regulate cell phenotype and tissue maturation, and have shown promise in cartilage regeneration. This review provides comprehensive guidelines for the rational design and applications of microcarriers in cartilage tissue engineering.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Xin Chen, Baoyu Tan, Shang Wang, Rongze Tang, Zhiteng Bao, Gaoyang Chen, Shuai Chen, Wanze Tang, Zhenmin Wang, Canling Long, William W. Lu, Dazhi Yang, Liming Bian, Songlin Peng
Summary: This study reported a smart hydrogel delivery system for bone repair, utilizing a T4 lysozyme mutant to mediate localized release of Mg2+ and Zn2+. The hydrogel showed excellent mechanical properties, antibacterial activity, and unique bioactivities, contributing to tissue regeneration.
Review
Orthopedics
Sida Liao, Haoye Meng, Junkang Li, Jun Zhao, Yichi Xu, Aiyuan Wang, Wenjing Xu, Jiang Peng, Shibi Lu
Summary: The application of microcarriers in cartilage regeneration has advanced significantly in recent years, providing a conducive microenvironment for cell expansion and enabling the delivery of proteins, factors, and drugs. They offer advantages like injectability and targeted delivery, overcoming limitations of traditional methods and enhancing efficiency and mechanical properties in combination with other stents.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Chemistry, Multidisciplinary
Sheng-Long Ding, Xi-Yuan Zhao, Wei Xiong, Lin-Feng Ji, Min-Xuan Jia, Yan-Yan Liu, Hai-Tao Guo, Feng Qu, Wenguo Cui, Qi Gu, Ming-Zhu Zhang
Summary: Cartilage equivalents from hydrogels containing chondrocytes show great potential in hyaline cartilage regeneration. However, current methods struggle to recreate the original architecture and culture non-differentiated chondrocytes in vitro. This study introduces specially designed microcarriers that use mechanotransductive conditions to form stable hyaluronic acid, contributing to the regeneration of hyaline cartilaginous microtissue and preventing chondrocyte dedifferentiation.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Mattia Vitale, Cosimo Ligorio, Bethan McAvan, Nigel W. Hodson, Chris Allan, Stephen M. Richardson, Judith A. Hoyland, Jordi Bella
Summary: Hydrogels have great potential for tissue engineering applications, but their use in bone regeneration is often hindered by poor mineralization and mechanical properties. Osteoclasts (OCs), which play a pivotal role in bone homeostasis, are often overlooked. This study aims to develop customized, reinforced hydrogels as a platform for studying OC function and differentiation.
ACTA BIOMATERIALIA
(2022)
Review
Materials Science, Multidisciplinary
Zhihua Zhou, Wei Wu, Jianjun Fang, Jingbo Yin
Summary: This article reviews important issues related to polymeric porous microcarriers for tissue engineering, including their properties, fabrication techniques, biomaterials, and applications in bone and cartilage tissue engineering.
INTERNATIONAL MATERIALS REVIEWS
(2021)
Article
Orthopedics
Spencer E. Szczesny, David T. Corr
Summary: A breakout session was held at the 2022 ORS Tendon Section Meeting to establish guidelines for studying tendon cell and tissue cultures. This paper summarizes the outcomes of the discussion and provides recommendations for future studies.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Review
Food Science & Technology
Nima Moslemy, Emad Sharifi, Mitra Asadi-Eydivand, Nabiollah Abolfathi
Summary: Cultured meat, produced by culturing animal cells in vitro, offers a promising solution to the challenges of traditional meat production. However, the industry still needs to overcome challenges such as scaling up production and ensuring product safety.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Review
Cell Biology
Venkata Suresh Venkataiah, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Yusuke Kakiuchi, Masato Nakano, Shigeto Suzuki, Keisuke Handa, Masahiro Saito
Summary: Bone tissue engineering involves combining MSCs with a biocompatible scaffold to aid in bone regeneration and recovery. While promising results have been seen in clinical trials, regenerating large bone defects remains a challenge.
Article
Veterinary Sciences
Zihang Ma, Xiaoying Guo, Jun Zhang, Qifeng Jiang, Wuying Liang, Wenxin Meng, Shuaijiang Chen, Yufan Zhu, Cundong Ye, Kun Jia
Summary: This study aimed to evaluate the therapeutic effect of combining canine bone marrow mesenchymal stem cells (cBMSCs) with gelatin-nano-hydroxyapatite (Gel-nHAP) on bone defect diseases in dogs. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In animal experiments, significant cortical bone growth was observed in the Gel-nHAP group at week 8 and in the cBMSCs-Gel-nHAP group at week 4.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Polymer Science
Lixia Huang, Yifei Jiang, Xinying Chen, Wenqi Zhang, Qiuchen Luo, Siyan Chen, Shuhan Wang, Fangqing Weng, Lin Xiao
Summary: Supramolecular responsive microcarriers based on chitosan microspheres were developed for nonenzymatic cell detachment. These microcarriers showed excellent biocompatibility and gelatin niches, allowing for effective cell attachment and expansion. Cells could be detached from the microcarriers through competitive binding.
Article
Chemistry, Physical
Javier Gil, Roman Perez, Mariano Herrero-Climent, Maria Rizo-Gorrita, Daniel Torres-Lagares, Jose Luis Gutierrez
Summary: This study compared three different surfaces of titanium dental implants and found that surfaces with residual alumina had lower surface free energy, accelerating bone tissue growth and exhibiting bactericidal effects.
Article
Materials Science, Multidisciplinary
Juan Carlos Vara, Juan Delgado, Alejandro Estrada-Martinez, Esteban Perez-Pevida, Aritza Brizuela, Begona Bosch, Roman Perez, Javier Gil
Summary: This study analyzed the detachment of particles in surrounding tissues caused by the friction between bone-level dental implants and cortical bone. The results showed that the Ti6Al4V alloy particles released a higher level of ions and had poorer cytocompatibility compared to commercially pure titanium, indicating a higher risk of inflammation.
Article
Medicine, General & Internal
Javier Gil, Clara Sandino, Miguel Cerrolaza, Roman Perez, Mariano Herrero-Climent, Blanca Rios-Carrasco, Jose Vicente Rios-Santos, Aritza Brizuela
Summary: The purpose of this study was to investigate the biomechanical response of dental implants in different positions and cortical bone thicknesses. Numerical simulations and in vivo experiments showed that implants placed 0.5 mm below the bone level with smaller thickness had the best performance in terms of osseointegration.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Elia Bosch-Rue, Leire Diez-Tercero, Luis M. Delgado, Roman A. Perez
Summary: Cardiovascular diseases, particularly atherosclerosis, are prevalent worldwide and often lead to death. Traditional treatment involves bypass surgery, but autologous sources are not always available. Tissue-engineered blood vessels (TEBVs) are emerging as potential alternative sources. This study presents a method for developing support-free collagen TEBVs through co-axial extrusion. The results demonstrate that the collagen TEBVs produced through this one-step procedure have sufficient mechanical properties to withstand perfusion and maintain cell viability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Leire Diez-Tercero, Luis M. Delgado, Roman A. Perez
Summary: This study investigated the synergistic effect of copper and magnesium ions combined with the anti-inflammatory drug dexamethasone (dex) on macrophages. It found that the combination of copper and dex significantly decreased the expression of pro-inflammatory markers, while the combination of magnesium and dex upregulated the expression of the anti-inflammatory cytokine IL-10. However, the combination of copper and dex in the presence of a pro-inflammatory stimulus induced a strong TNF-alpha response, suggesting an impairment of the anti-inflammatory actions of dex.
Article
Biochemistry & Molecular Biology
Xavier Marimon, Miguel Cerrolaza, Miquel Ferrer, Oriol Canto-Naves, Josep Cabratosa-Termes, Roman Perez
Summary: This study aimed to determine the best mechanical characteristics of restorative materials for manufacturing implant crowns subjected to impact loading. Different combinations of external and internal crown restorative materials were analyzed, and the dynamic stresses at eight different zones of a dental implant were analyzed and compared. A 3D model was created and analyzed using finite element analysis, and the results allowed for evaluating the effect of different materials and mechanical properties on the stress distribution. The study emphasized the importance of selecting the appropriate restorative material for preserving the integrity of osseointegration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Ceramics
Raquel Rodriguez-Gonzalez, Elia Bosch-Rue, Leire Diez-Tercero, Luis M. Delgado, Roman A. Perez
Summary: Sol-gel silica-based hybrids can be tailored for tissue regeneration by adjusting their properties. Factors such as water content, TEOS:GPTMS volume ratio, and gelatin:sol volume ratio during the sol-gel preparation process influence the material's performance. Higher water content reduces degradation rate and mechanical properties, while decreasing water uptake. Increasing the amount of cross-linker in the TEOS:GPTMS volume ratio slightly decreases water uptake and degradation rate, and improves mechanical properties. Lastly, the gelatin:sol volume ratio affects water uptake, degradation rate, and mechanical properties.
CERAMICS INTERNATIONAL
(2022)
Article
Chemistry, Physical
Barbara Giordano-Kelhoffer, Raquel Rodriguez-Gonzalez, Marina Perpinan-Blasco, Jenifer O. Buitrago, Begona M. Bosch, Roman A. Perez
Summary: Bone grafting is a commonly used treatment for bone healing or repair. Autografts are frequently used because they can provide osteogenic cells and growth factors with reduced risk of rejection or disease transfer. However, autografts have limitations such as pain and limited availability. Synthetic bone grafts are proposed as an alternative to overcome these drawbacks.
Review
Engineering, Biomedical
Elia Bosch-Rue, Leire Diez-Tercero, Jenifer Olmos Buitrago, Emilio Castro, Roman A. Perez
Summary: Bone has the ability to self-regenerate, but extensive damage can hinder the natural regeneration process. Bone tissue engineering aims to mimic the extracellular matrix and promote the physiological regeneration process by stimulating osteogenesis and angiogenesis.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Maria Merino-Gomez, Javier Gil, Roman A. Perez, Maria Godoy-Gallardo
Summary: Tissue engineering aims to develop materials that can be used to regenerate, repair, or replace damaged tissues. 3D printing has shown promise for producing tailored implants, leading to an increased demand for new inks and bioinks. Supramolecular hydrogels based on nucleosides, particularly guanosine, have gained attention due to their biocompatibility and self-healing capabilities. However, existing formulations lack stability, biological activity, or printability. To overcome these limitations, we incorporated polydopamine into guanosine-borate hydrogels, resulting in a PGB hydrogel with improved properties. The PGB hydrogel exhibited good mechanical characteristics, increased osteogenic activity, and antimicrobial properties. This study suggests that the PGB hydrogel is a promising candidate for 3D-printed scaffolds that can support living cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Esteban Padulles-Gaspar, Esteban Padulles-Roig, Guillermo Cabanes, Roman A. Perez, Javier Gil, Begona M. Bosch
Summary: One of the main problems in oral implantology is peri-implantitis, which affects almost 20% of dental implants placed in patients. This study evaluates the use of chemical treatments using hypochlorous acid (HClO) and hydrogen peroxide (H2O2) for decontamination of titanium surfaces. The results show that while the treatment can effectively remove contaminants, it also generates roughness that may negatively impact long-term performance.
Article
Chemistry, Physical
Raquel Rodriguez-Gonzalez, Loreto Monsalve-Guil, Alvaro Jimenez-Guerra, Eugenio Velasco-Ortega, Jesus Moreno-Munoz, Enrique Nunez-Marquez, Roman A. Perez, Javier Gil, Ivan Ortiz-Garcia
Summary: The influence of surface topography on dental implants was studied to improve osseointegration. Different treatments were applied to titanium samples, and the roughness, wettability, surface energy, cell adhesion, and bacterial proliferation were examined. The results showed that rough surfaces had higher cell adhesion and osteocalcin levels, while bacterial proliferation was not significantly affected.
Article
Medicine, General & Internal
Andoni Carrasco-Uribarren, Xavier Marimon, Alejandro Portela, Sara Cabanillas-Barea, Pere Ramon Rodriguez-Rubio, Roman A. Perez
Summary: This study describes a novel device, called Spinetrack, designed to assess the cervical spine and evaluates its test-retest reliability. The device measures the strength of deep cervical flexor muscles and the chin-in and chin-out movement of the upper cervical spine. Results demonstrate excellent test-retest reliability for measuring cervical flexor muscle strength and chin-in and chin-out movements.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Maximilian Jergitsch, Zoe Allu Allue-Mengual, Roman A. Perez, Miguel A. Mateos-Timoneda
Summary: This study explores the effect of different salt, sucrose, and MC concentrations on the printability of methylcellulose hydrogels. Increasing G' improves shape fidelity, while lowering tan(delta) improves shape fidelity as well. Lowering MC concentration, extrusion rate, and nozzle gauge can increase cell viability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Materials Science, Biomaterials
Begona M. Bosch, Elia Bosch-Rue, Marina Perpinan-Blasco, Roman A. Perez
Summary: Corneal endothelium defects are a major cause of blindness worldwide. The current treatment is transplantation, but there is a global shortage of donors. Cell therapy, combined with extracellular mimicking materials, has shown promising results in tissue regeneration. In this review, different methods to obtain corneal endothelial cells from various sources are discussed, along with substrates to promote successful implantation and enhanced cell behavior. Future directions include 3D printing for patient-specific substrates.
REGENERATIVE BIOMATERIALS
(2022)
