Review
Chemistry, Multidisciplinary
Fernando J. Aguilar-Ayala, Fernando J. Aguilar-Perez, Geovanny Nic-Can, Rafael Rojas-Herrera, Gabriela Chuc-Gamboa, David Aguilar-Perez, Beatriz A. Rodas-Junco
Summary: Biomaterials and stem cells are crucial in regenerative medicine, particularly in the development of autologous therapies. While progress has been made, challenges remain in understanding the characteristics of biomaterials and the molecular mechanisms regulating stem cell behavior.
APPLIED SCIENCES-BASEL
(2022)
Review
Biochemistry & Molecular Biology
David Muallah, Jan Matschke, Matthias Kappler, Lysann Michaela Kroschwald, Gunter Lauer, Alexander W. Eckert
Summary: Xerostomia, or dry mouth, is mainly caused by hypofunction of the salivary glands due to various factors. Current treatment methods are not very effective and regenerative medicine using dental pulp stem cells shows promise. Dental pulp stem cells can differentiate into different cell types and have immunomodulatory effects. However, more clinical studies are needed to fully understand their potential for salivary gland regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Raphael J. Crum, Hector Capella-Monsonis, Stephen F. Badylak, George S. Hussey
Summary: Tissue engineering and regenerative medicine focus on developing new techniques, devices, and materials to replace or repair injured or diseased tissue and organs. These approaches use cells, scaffolds, and signaling molecules to promote repair and regeneration. Extracellular vesicles have emerged as key mediators of tissue regeneration and repair.
APPLIED SCIENCES-BASEL
(2022)
Article
Engineering, Biomedical
Otto J. Juhl, Anna-Blessing Merife, Yue Zhang, Henry J. Donahue
Summary: This study investigates the mechanism by which substrate surface characteristics are transduced by osteoblastic cells. The results suggest that focal adhesion and beta-catenin play a role in this mechanism by liberating beta-catenin from focal adhesion complexes, which then facilitates upregulation of osteogenesis-associated genes. Inhibiting focal adhesion turnover mitigates the increase in beta-catenin translocation and osteoblastic differentiation induced by pro-osteogenic substrate.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Qiulin He, Junxin Lin, Fanghao Zhou, Dandan Cai, Yiyang Yan, Yejie Shan, Shufang Zhang, Tiefeng Li, Xudong Yao, Hongwei Ouyang
Summary: Nonuniform microstretching (NUMS), a natural phenomenon in bone tissues, promotes osteogenic differentiation and has potential positive effects on bone healing.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Charles M. Bowen, Frederick S. Ditmars, Ashim Gupta, Jo-Anna Reems, William Samuel Fagg
Summary: Amniotic fluid, specifically its cell-free form, has regenerative properties and can stimulate immunomodulatory and pro-growth characteristics in target cells or tissues. It has been shown to effectively treat cutaneous wounds and orthopedic degenerative conditions.
Article
Engineering, Biomedical
Bin Wang, Qinglai Tang, Qian Yang, Mengmeng Li, Shiying Zeng, Xinming Yang, Zian Xiao, Xinying Tong, Lanjie Lei, Shisheng Li
Summary: Due to their unique properties such as low immunogenicity, biomimetic internal environment, tissue-and organ-like physicochemical properties, and functionalization potential, decellularized extracellular matrix (dECM) materials have gained considerable attention in tissue engineering. This review provides an overview of the composition of extracellular matrices and their role in stem-cell differentiation, discusses the advantages and disadvantages of existing decellularization techniques, and presents methods for the functionalization and characterization of decellularized scaffolds. Additionally, the review discusses the progress and shortcomings of using dECMs in cartilage, skin, nerve, and muscle repair, as well as the transplantation or regeneration of different whole organs, and examines the future prospects of refunctionalization in tissue and organ repair. It aims to systematize the application of functionalized dECMs in tissue/organ transplantation and keep researchers informed about the latest progress in dECM usage.
MATERIALS TODAY BIO
(2023)
Review
Biochemistry & Molecular Biology
Victor J. Costela-Ruiz, Lucia Melguizo-Rodriguez, Chiara Bellotti, Rebeca Illescas-Montes, Deborah Stanco, Carla Renata Arciola, Enrico Lucarelli
Summary: The success of regenerative medicine depends on the appropriate selection of mesenchymal stem cell (MSC) sources, as they influence the regenerative milieu. MSCs from bone marrow, adipose tissue, and dental tissue are all potential candidates for oral and dental regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell & Tissue Engineering
Jing-Yao Yin, Xing-Hong Luo, Wei-Qing Feng, Sheng-Hong Miao, Ting-Ting Ning, Qian Lei, Tao Jiang, Dan-Dan Ma
Summary: Tooth-related diseases and tooth loss are common public health issues, leading to the emergence of tooth regeneration science. Dental mesenchymal stem cells (DMSCs) play a significant role in tooth regeneration due to their undifferentiated state and multipotency, offering potential for reconstructing complete tooth tissues with normal functions and vascularization. Researchers aim to utilize the differentiation potential of DMSCs towards various tissue types to improve regenerative medicine and tissue engineering applications.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Engineering, Biomedical
Vishnu Priya Murali, Christina A. Holmes
Summary: This review summarizes various biomaterial-based extracellular vesicle (EV) delivery systems used in preclinical tissue regeneration studies and other therapeutic applications. EV-based therapies have shown success in preclinical models for biomedical applications, but the challenge lies in prolonging EV retention at target sites for improved therapeutic efficacy through biomaterial-based systems.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Hu Qian, Li He, Zhimin Ye, Zairong Wei, Jun Ao
Summary: Intervertebral disc degeneration (IDD) is a common cause of low back pain and poses a burden on public health systems worldwide. Current treatment options, including conservative and surgical approaches, are ineffective in restoring intervertebral disc (IVD) function. Decellularized matrix (DCM), a tissue-engineered biomaterial, has shown promise in IVD regeneration due to its retention of extracellular matrix (ECM) components. Significant progress has been made in the preparation process, mechanistic insights, and application of DCM for IDD repair. This comprehensive review summarizes the roles and applications of DCM in IDD repair, providing novel insights for clinical treatment.
MATERIALS TODAY BIO
(2023)
Review
Biochemistry & Molecular Biology
Priti Gupta, Shilpa Sharma, Shagufta Jabin, Sapana Jadoun
Summary: Regenerative medicine and tissue engineering have great potential in addressing the limitations of traditional medical approaches. Chitosan, as a naturally derived biocompatible biomaterial, has played a significant role in tissue regeneration. It has unique properties and can be applied in various fields, such as agriculture, nutraceuticals, biomedicine, and food. Chitosan is also an important material for developing next-generation hydrogels and bio-scaffolds. Furthermore, chitosan can be easily modified to enhance its mechanical strength, biodegradability, and controlled release of bioactive molecules. Blending chitosan with other polymers or incorporating nanoparticles expands its possibilities in tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Cell Biology
Babak Arjmand, Mina Abedi, Maryam Arabi, Sepideh Alavi-Moghadam, Mostafa Rezaei-Tavirani, Mahdieh Hadavandkhani, Akram Tayanloo-Beik, Ramin Kordi, Peyvand Parhizkar Roudsari, Bagher Larijani
Summary: Cardiovascular disease is the leading cause of adult death worldwide, with myocardial infarction accounting for four fifths of cardiovascular disease deaths. Regenerative medicine, particularly using stem cells to repair the heart, has shown promising benefits as a treatment for ischemic heart disease. Despite the potential of stem cell therapy, more research is needed to fully understand its mechanism of action and its long-term effects on cardiovascular disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Burak Toprakhisar, Catherine M. Verfaillie, Manoj Kumar
Summary: Liver transplantation is currently the only curative therapy for acute or chronic liver failure, but the shortage of available liver grafts highlights the need for valid liver substitutes. Whole-organ engineering, a field of tissue engineering and regenerative medicine, aims to generate transplantable and functional organs for patients on transplantation waiting lists. This review focuses on recent advancements in organ decellularization and scaffold recellularization technologies for liver whole-organ engineering, including potential sources of liver cells, cell seeding strategies, graft modifications, and functional evaluation methods. Future directions based on transplantation studies are also summarized.
Article
Engineering, Biomedical
Junmin Lee, Aly Ung, Hanjun Kim, KangJu Lee, Hyun-Jong Cho, Praveen Bandaru, Samad Ahadian, Mehmet R. Dokmeci, Ali Khademhosseini
Summary: Increasing evidence suggests that fusion of cancer cells with different cell types in the tumor microenvironment may contribute to the generation of metastasis-initiating cells. The role of human mesenchymal stem cells (hMSCs) in fusion with cancer cells is still controversial. In this study, a liver-on-a-chip platform was used to investigate the fusion of liver hepatocellular cells (HepG2) with hMSCs and their invasive potential. It was found that hMSCs may play dual roles in HepG2 spheroids, preventing HepG2 growth while also leading to the generation of highly invasive HepG2-hMSC hybrid cells. These hybrid cells expressed markers associated with stemness, proliferation, epithelial to mesenchymal transition, and matrix deposition, and were responsible for collective invasion following HepG2.
Article
Engineering, Biomedical
Anant Bhusal, Elvan Dogan, Hai-Anh Nguyen, Olga Labutina, Daniel Nieto, Ali Khademhosseini, Amir K. Miri
Summary: This study developed a multi-material DLP-based bioprinter for rapid prototyping of hydrogel-based microfluidic chips. The optimized composite hydrogel bioink allows for a wide range of mechanical properties. The biofabrication approach offers a useful tool for integrating micro-tissue models into organs-on-chips and high-throughput drug screening platforms.
Review
Biotechnology & Applied Microbiology
Maryam Tavafoghi, Fatemeh Nasrollahi, Solmaz Karamikamkar, Mahboobeh Mahmoodi, Sara Nadine, Joao F. Mano, Mohammad A. Darabi, Jamileh Jahangiry, Samad Ahadian, Ali Khademhosseini
Summary: Microneedles (MNs) have been developed as minimally invasive tools for diagnostic and therapeutic applications. There is increasing interest in developing smart multifunctional MN devices for body fluid extraction, biosensing, and drug delivery. The main challenge is integrating multiple modules, such as drug carriers and biosensors, in one miniaturized MN device. Researchers have shown the feasibility of creating smart MNs using biomaterials and microscale technologies. Hydrogel-based MN devices show high potential for biomedical applications. The improvement of biomaterials knowledge and biofabrication techniques will allow the development of more effective personalized therapeutics.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Chemistry, Multidisciplinary
Hossein Montazerian, Elham Davoodi, Avijit Baidya, Sevana Baghdasarian, Einollah Sarikhani, Claire Elsa Meyer, Reihaneh Haghniaz, Maryam Badv, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Hemostatic biomaterials have great potential in wound control, particularly for uncontrolled bleeding associated with damaged tissues, traumatic wounds, and surgical incisions. Current research focuses on enhancing the hemostatic properties of bioactive materials through triggering the coagulation cascade, using biocompatible and biodegradable materials for rapid blood coagulation, and developing tough bioadhesives for efficient incision sealing.
Article
Chemistry, Multidisciplinary
Dayi Jeong, Jeong Wook Seo, Hong-Gu Lee, Woo Kyung Jung, Yong Ho Park, Hojae Bae
Summary: The interest in cultured meat is growing due to issues with conventional livestock industry. This study introduces 3D bioprinting for producing large cell aggregates for cultured meat production, and successfully creates scaffolds with living cells and large microchannels using bioinks.
Review
Pharmacology & Pharmacy
Serge Ostrovidov, Murugan Ramalingam, Hojae Bae, Gorka Orive, Toshinori Fujie, Xuetao Shi, Hirokazu Kaji
Summary: With the advances in skeletal muscle tissue engineering, new platforms have emerged for biology studies, disease modeling, and drug testing. The authors review the latest advances in in vitro models of engineered skeletal muscle tissues used for drug testing, focusing on four main cell culture techniques: well plate cultures, microfluidics, organoids, and bioprinted constructs. More developments are expected to increase the validation and use of these models in drug testing.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Materials Science, Biomaterials
Marvin Mecwan, Reihaneh Haghniaz, Alireza Hassani Najafabadi, Kalpana Mandal, Vadim Jucaud, Johnson V. John, Ali Khademhosseini
Summary: Researchers have successfully engineered a thermoresponsive shear-thinning hydrogel, composed of a thermoresponsive polymer and hemostatic silicate nanodisks, as an injectable hemostat. This hydrogel demonstrates rapid mechanical recovery and temperature-dependent blood coagulation, effectively preventing blood loss. It shows comparable efficacy to a commercially available hemostat and can be easily removed using a cold saline wash.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Gang Ge, Kalpana Mandal, Reihaneh Haghniaz, Mengchen Li, Xiao Xiao, Larry Carlson, Vadim Jucaud, Mehmet Remzi Dokmeci, Ghim Wei Ho, Ali Khademhosseini
Summary: Adhesive materials have been receiving increasing attention for their excellent sealing ability. However, existing adhesives typically have weak adhesion strength and deteriorate in harsh environments. In this study, a novel ionogel with tunable mechanical properties is developed using a deep eutectic solvent as the medium for photopolymerization. The ionogel demonstrates fast gelation and bonding without external pressure, high adhesion in various environments, and wide applications in packaging, marine engineering, medical adhesives, and electronic assembly.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell & Tissue Engineering
Pauline Cheng, Ahmad Rashad, Ankit Gangrade, Natan Roberto de Barros, Ali Khademhosseini, Jonathan Tam, Padmini Varadarajan, Devendra K. Agrawal, Finosh G. Thankam
Summary: Myocardial infarction leads to the loss of cardiomyocytes, but stem cell therapy shows promise in restoring cardiac function. This article discusses the application of different stem cell phenotypes and strategies to promote their differentiation into cardiomyocytes.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Engineering, Biomedical
Hossein Montazerian, Alireza Hassani Najafabadi, Elham Davoodi, Rasoul Seyedmahmoud, Reihaneh Haghniaz, Avijit Baidya, Wei Gao, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Mussel-inspired catechol-functionalization of degradable natural biomaterials is proposed as a potential approach to achieve bioadhesion for sutureless wound closure. This approach involves a simple oxidative polymerization step before conjugation of catechol-carrying molecules to amplify catechol function in bioadhesion. The modified gelatin with poly(l-DOPA) moieties shows improved wound control and enhanced cohesion, surpassing commercial sealants, and also possesses photothermal responsiveness and antibacterial activity, making it an effective biomaterial design strategy for wound closure applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Hossein Montazerian, Shameek Mitra, Alireza Hassani Najafabadi, Rasoul Seyedmahmoud, Yuting Zheng, Mehmet Remzi Dokmeci, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Inspired by mussel adhesion, catechol functionalization has been widely used to impart adhesion to biomaterials. However, the bioadhesion capacity of catechol motifs in hydrogels that crosslink through free-radical-based systems is debated. This study reevaluates the bioadhesion efficacy of catechol functionalized gelatin biomolecules in methacryloyl-modified photo-cross-linkable biomaterials, and investigates the effects of catechol content on adhesion strength and hydrogel integrity. The cytotoxic and immunostimulatory effects of catechol groups are also evaluated for clinical applications.
ACS MATERIALS LETTERS
(2023)
Article
Chemistry, Analytical
Safoora Khosravi, Saeid Soltanian, Amir Servati, Ali Khademhosseini, Yangzhi Zhu, Peyman Servati
Summary: Wearable sweat biosensors embedded in textile substrates have gained attention for noninvasive monitoring of health parameters. A flexible electrochemical glucose sensor that can be screen-printed onto a textile substrate is demonstrated, achieving a linear response in the range of 20-1000μM of glucose concentration with high sensitivity and stability. These textile-based biosensors show high selectivity towards glucose and have the potential to impact the next generation of wearable devices.
Review
Automation & Control Systems
Baishali Kanjilal, Yangzhi Zhu, Vaishali Krishnadoss, Janitha M. M. Unagolla, Parnian Saemian, Alessia Caci, Danial Cheraghali, Iman Dehzangi, Ali Khademhosseini, Iman Noshadi
Summary: Ionic liquids (ILs) have unique properties that make them promising candidates for biomedical applications, but their cytocompatibility limitations are enhanced by using bioionic liquids (BILs) derived from biological molecules. BILs can be synthesized and immobilized onto biopolymers, and their functionalization enables the design of responsive actuators and sensors. The cytocompatibility of BIL-functionalized polymers also makes them suitable for power storage and implantable devices. This review focuses on the recent advances of BILs in biomedical applications, specifically their use as functionalization agents for biopolymers and solvents for supermolecular ionic networks.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Chemistry, Multidisciplinary
Bruna Gregatti Carvalho, Aya Nakayama, Hiromi Miwa, Sang Won Han, Lucimara Gaziola de la Torre, Dino Di Carlo, Junmin Lee, Han-Jun Kim, Ali Khademhosseini, Natan Roberto de Barros
Summary: A novel mRNA-releasing matrix based on GelMA microporous annealed particle scaffolds is reported. The sustained release of mRNA complexes achieves indirect intracellular delivery, while direct intracellular delivery is achieved by cell adhesion on the mRNA-releasing scaffolds. This hybrid system demonstrates efficient protein expression, offering potential for mRNA-releasing biomaterials in tissue engineering.
Review
Chemistry, Multidisciplinary
Hossein Montazerian, Elham Davoodi, Avijit Baidya, Maryam Badv, Reihaneh Haghniaz, Arash Dalili, Abbas S. Milani, Mina Hoorfar, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: This review provides a biomacromolecular design roadmap for the development of tough adhesive surgical sealants. The intrinsic toughness and elasticity of polymers are achieved through the introduction of strong and dynamic inter- and intramolecular interactions, either through polymer chain design or the use of crosslink regulating additives. Efforts have also been made to promote underwater adhesion through covalent/noncovalent bonds and micro/macro-interlock mechanisms. The measurement and reporting requirements for fair comparisons of different materials and their properties are discussed.
CHEMICAL SOCIETY REVIEWS
(2022)
