Article
Biophysics
Yang Yang, Alastair Campbell Ritchie, Nicola M. Everitt
Summary: Recombinant human collagen polypeptide (RHC) and RHC-chitosan (RHC-CHI) porous scaffolds with high porosity and adjustable mechanical properties show excellent in vitro cytocompatibility. The porous scaffolds promote cell attachment, proliferation, and synthesis of extra cellular matrix-associated proteins in vitro. In vivo studies demonstrate that these scaffolds accelerate cell infiltration and collagen deposition, facilitating wound closure.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Materials Science, Biomaterials
Jamie A. Cyr, Maria Colzani, Semih Bayraktar, Maria Kohne, Daniel V. Bax, Vera Graup, Richard Farndale, Sanjay Sinha, Serena M. Best, Ruth E. Cameron
Summary: Regenerative cardiac tissue is a promising field for myocardial repair. Scaffold with similar structure to native myocardium improves tissue function. Our study isolates the influence of scaffold macrostructure on tissue function and provides insights for optimized cardiac tissue regeneration and medical system design.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
M. Ethan Brown, Jennifer L. L. Puetzer
Summary: This study investigates the effect of compressive mechanical boundary conditions and the addition of beta-tricalcium phosphate (beta TCP) on the development of zonal ligament-to-bone entheses. The results show that compressive boundary clamps and beta TCP can guide ligament fibroblasts to produce zonal gradients in collagen organization, mineralization, and matrix composition, closely resembling immature ligament-to-bone attachments. This culture system provides a promising platform for understanding and promoting enthesis regeneration in graft repair and engineered replacements.
ACTA BIOMATERIALIA
(2022)
Review
Chemistry, Medicinal
Jonathan Ardhianto Panggabean, Sya'ban Putra Adiguna, Mutia Hardhiyuna, Siti Irma Rahmawati, Nina Hermayani Sadi, Gunawan Pratama Yoga, Eva Nafisyah, Asep Bayu, Masteria Yunovilsa Putra
Summary: Aquatic-based collagens have great potential in biomedical applications, especially in tissue engineering, due to their superior characteristics compared to mammalian-based collagen. They are biocompatible, biodegradable, easy to extract, and pose a low immunological risk. The utilization of aquatic-based collagen also offers advantages such as fewer religious restrictions and lower production costs, while promoting environmental sustainability through aquatic waste utilization.
Review
Biochemistry & Molecular Biology
Jernej Vajda, Marko Milojevic, Uros Maver, Bostjan Vihar
Summary: The review highlights the current state of microvascular tissue engineering, outlining the challenges faced in developing microvasculature and proposing potential strategies for overcoming these obstacles in future research.
Article
Chemistry, Applied
Sihan Zhang, Guanglei Zhao, Wei Ma, Yanghui Song, Cheng Huang, Chong Xie, Kebing Chen, Xiaofeng Li
Summary: A porous chitosan nanofiber scaffold (CSNFS) with an extracellular matrix-like structure was prepared through a cross-linking reaction, showing high porosity and adequate micro-scale pores. CSNFS has good mechanical strength and biocompatibility, and can be used as a repair material for bone tissue engineering.
CARBOHYDRATE POLYMERS
(2022)
Article
Biotechnology & Applied Microbiology
Florian Helms, Sarah Zippusch, Jonathan Theilen, Axel Haverich, Mathias Wilhelmi, Ulrike Boeer
Summary: This study developed a bioartificial tissue construct with a fibrin matrix containing human umbilical vein endothelial cells and adipose tissue-derived stem cells, facilitating the formation of a capillary-like network. The construct provided biomechanical stability and could be used for tissue engineering with different cell types.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Engineering, Environmental
WonJin Kim, Hyeongjin Lee, Eun Ji Roh, Seong Bae An, In-Bo Han, Geun Hyung Kim
Summary: The bioprinted cell-laden construct consisting of collagen, beta-tricalcium phosphate, human adipose stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs) was successfully used for bone tissue regeneration in spinal fusion. The construct exhibited strong angiogenic and osteogenic activities due to the synergistic crosstalk of the two cell types, leading to significantly higher new bone formation and angiogenesis in a mouse model of posterolateral lumbar spinal fusion. These findings suggest that biomimetic cell-laden constructs could potentially encourage effective spinal fusion by promoting bone tissue regeneration.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Polymer Science
Kazuya Furusawa, Yuuki Kawahana, Ryoya Miyashita
Summary: Tissue engineering methods have been developed to mimic the hierarchical structure of skeletal muscle tissue for applications in biology, and a method is needed to reproduce the macroscopic morphology. In this study, an engineered muscle tissue (EMT) was created by culturing embryonic chicken myoblast-like cells in collagen hydrogels with micro-channels, resulting in unidirectionally aligned myotubes. The EMT exhibited anisotropic elasticity and relaxation strengths, attributed to differences in maturation and connectivity of myotubes.
Review
Biotechnology & Applied Microbiology
Britani N. Blackstone, Summer C. Gallentine, Heather M. Powell
Summary: This systematic review examines the use of electrospun collagen in tissue engineering applications, finding that different crosslinking methods can affect degradation resistance and strength of the scaffolds, which are capable of forming engineered tissues with high similarity to native structures.
BIOENGINEERING-BASEL
(2021)
Article
Engineering, Biomedical
Madison Jade Ainsworth, Nino Chirico, Mylene de Ruijter, Andrei Hrynevich, Inge Dokter, Joost P. G. Sluijter, Jos Malda, Alain van Mil, Miguel Castilho
Summary: In order to develop thick engineered cardiac tissue construct with a pre-vascularization pathway, a combination of extrusion-based bioprinting and melt electrowriting techniques was investigated. Gelatin methacryloyl (GelMA) was used as a base hydrogel and collagen, Matrigel, and fibrinogen were utilized as interpenetrating polymers to support myocardial functionality. An anatomically inspired vascular pathway was implemented in a dual extrusion-based bioprinting setup. The optimized bioinks effectively filled the microfiber mesh and maintained cell viability, and distinct cell populations were visualized in their respective patterns up to D14.
Review
Polymer Science
Hsiuying Wang
Summary: Collagen, with its various applications in pharmaceuticals, medicine, food, and cosmetics, has been shown to benefit human health, particularly for aging individuals. Clinical studies have demonstrated significant effects of collagen treatment across various diseases, suggesting a potential correlation between collagen deficiency and comorbid conditions, with collagen treatment being a valuable option for patients with multiple diseases, including COVID-19.
Article
Chemistry, Multidisciplinary
Dominic Rutsche, Monica Nanni, Simon Rudisser, Thomas Biedermann, Marcy Zenobi-Wong
Summary: This study introduces a new collagen derivative with multiple recognition peptides for enzyme-mediated crosslinking, enabling the co-engineering of human blood and lymphatic microcapillaries. The study further demonstrates the formation of luminal structures and the maturation markers of bioengineered capillaries both in vitro and in vivo. The versatile collagen derivative is also utilized for spatially defined polymer compositions with pro- and anti-angiogenic properties, as well as the formation of vascularization independent from fibroblasts.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
In-Kyu Lee, Ruosen Xie, Agustin Luz-Madrigal, Seunghwan Min, Jingcheng Zhu, Jiahe Jin, Kimberly L. Edwards, M. Joseph Phillips, Allison L. Ludwig, David M. Gamm, Shaoqin Gong, Zhenqiang Ma
Summary: Age-related macular degeneration (AMD) can lead to blindness by causing the loss of retinal pigment epithelium (RPE) and photoreceptors (PRs), which are the outermost layers of the retina. To reconstruct the outer retina, especially in later stage AMD and other macular dystrophies, scaffolds have been developed. Microfabricated, honeycomb-patterned, biodegradable poly(glycerol sebacate) (PGS) scaffolds were created to deliver organized layers of RPE and PRs to the subretinal space, and an optimized process was developed to shorten scaffold production time. These scaffolds successfully supported the seeding of human pluripotent stem cell-derived RPE and PRs, either separately or as a dual cell-layered construct. They have the potential to accelerate retinal cell transplantation efforts and benefit patients with AMD and other retinal degenerative diseases.
BIOACTIVE MATERIALS
(2023)
Article
Polymer Science
Kiran M. Ali, Yihan Huang, Alaowei Y. Amanah, Nasif Mahmood, Taylor C. Suh, Jessica M. Gluck
Summary: This study evaluates the potential use of traditional textile methods in the tissue engineering industry and proves the safety and efficacy of the material and fabrication method by creating collagen yarns using the traditional ring spinning method. The process is not only simple and efficient but also results in standardized products.
Article
Engineering, Biomedical
Bin Ma, Ming Li, Sabine Fuchs, Iris Bischoff, Alexander Hofmann, Ronald E. Unger, Charles J. Kirkpatrick
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Dentistry, Oral Surgery & Medicine
C. Herrera-Vizcaino, S. Al-Maawi, R. Sader, C. J. Kirkpatrick, J. Choukroun, Shahram Ghanaati
CLINICAL ORAL INVESTIGATIONS
(2020)
Article
Oncology
Laura Anspach, Roman Tsaryk, Larissa Seidmann, Ronald E. Unger, Caren Jayasinghe, Nektaria Simiantonaki, C. James Kirkpatrick, Felicitas Proels
JOURNAL OF PATHOLOGY
(2020)
Article
Orthopedics
David Grevenstein, Andreas Mamilos, Volker H. Schmitt, Tanja Niedermair, Willi Wagner, C. James Kirkpatrick, Christoph Brochhausen
Summary: The study analyzed histological and immunohistological findings from five patients who underwent spheroid-based autologous chondrocyte transplantation after traumatic lesions of the knee, showing excellent histological results in terms of hyaline articular cartilage regeneration. This indicates that spheroid-based autologous chondrocyte transplantation is a promising strategy for treating traumatic lesions of the knee's articular cartilage.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Anna Maria Tanneberger, Sarah Al-Maawi, Carlos Herrera-Vizcaino, Anna Orlowska, Alica Kubesch, Robert Sader, C. J. Kirkpatrick, Shahram Ghanaati
Summary: The study characterized the cellular reaction to a xenogeneic resorbable collagen membrane of porcine origin in Wistar rats over 30 days. It was found that the membrane induced a large number of mononuclear cells, including lymphocytes, macrophages, and fibroblasts, as well as multinucleated giant cells (MNGCs) expressing specific markers. The significant increase in MNGC number correlated with a high rate of vascularization compared to the control group, suggesting a potential role for MNGCs in biomaterial degradation.
CLINICAL ORAL INVESTIGATIONS
(2021)
Review
Immunology
Ignaz Wessler, Charles James Kirkpatrick
INTERNATIONAL IMMUNOPHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Susana G. Guerreiro, Ronald E. Unger, Nuno M. F. S. A. Cerqueira, Anne Sartoris, Maria J. Martins, Mario A. Barbosa, Raquel Soares, Pedro L. Granja, Charles J. Kirkpatrick
Summary: The study found that cocultures of fibroblasts and human endothelial cells stimulate collagen synthesis and growth factors production by fibroblasts, ultimately affecting the formation and distribution of microvessel-like structures in cell cultures. Areas with activated fibroblasts and high alkaline phosphatase (ALP) activity were also observed in the cocultures, indicating the impact of ALP on collagen proteins' aggregation, cell migration, and microvessel assembly. These findings suggest that bioinformatics and coculture systems are valuable tools for investigating the involvement of proteins like collagen and ALP in angiogenesis.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Chemistry, Physical
Samuel Ebele Udeabor, Carlos Herrera-Vizcaino, Robert Sader, C. James Kirkpatrick, Sarah Al-Maawi, Shahram Ghanaati
Article
Cell & Tissue Engineering
Eva Dohle, Andreas Scherrieble, Michael Doser, Sarah Al-Maawi, Martin Hoss, Martin Dauner, Robert Sader, Charles James Kirkpatrick, Shahram Ghanaati
TISSUE ENGINEERING PART C-METHODS
(2020)
Article
Oncology
Cristina L. Cotarelo, Annemarie Zschoeck-Manus, Marcus Schmidt, Arno Schad, Christoph Brochhausen, Charles James Kirkpatrick, Sonja Thaler
Summary: The intraoperative method combining touch imprint cytology (TIC) and subsequent frozen section (FS) analysis is highly reliable for detecting SLN macrometastases, allowing accurate identification of patients at high risk for extended axillary involvement during surgery and for accurate histological diagnosis of negative SLN.
CLINICAL & EXPERIMENTAL METASTASIS
(2021)
Article
Engineering, Biomedical
Sarah Al-Maawi, Mike Barbeck, Carlos Herrera Vizcaino, Rainer Egli, Robert Sader, Charles James Kirkpatrick, Marc Bohner, Shahram Ghanaati
Summary: The biological response of calcium phosphates is influenced by the presence of an alkaline layer on their surface during thermal treatment. Thermally treated alpha-TCP and beta-TCP granules stimulate fewer multinucleated giant cells and are more resistant to degradation compared to non-thermally treated granules. Heat treatment of alpha-TCP and beta-TCP granules shifts the reaction from foreign body response to physiological response, by reducing the number of induced multinucleated giant cells and enhancing degradation resistance.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Physical
Sarah Al-Maawi, Xuejiu Wang, Robert Sader, Werner Goetz, Antonella Motta, Claudio Migliaresi, Charles James Kirkpatrick, Shahram Ghanaati
Summary: This study analyzed the long-term polarization pattern of macrophages and multinucleated giant cells (MNGCs) in the implantation areas of biomaterials, and found sustained expression of pro-inflammatory markers in SF-induced MNGCs over 180 days. This highlights the importance of tailored physicochemical properties to avoid extensive inflammatory reactions for clinical success.
Article
Biochemistry & Molecular Biology
Thanuja D. K. Herath, Leonardo Saigo, Benoit Schaller, Anis Larbi, Swee Hin Teoh, Charles James Kirkpatrick, Bee Tin Goh
Summary: This study showed that autologous neutrophils may have a positive effect on promoting new bone formation, providing potential clinical benefits for bone regeneration in oral and maxillofacial surgery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Sarah Al-Maawi, Sandra Rother, Norbert Halfter, Karen M. Fiebig, Juliane Moritz, Stephanie Moeller, Matthias Schnabelrauch, Charles James Kirkpatrick, Robert Sader, Hans-Peter Wiesmann, Dieter Scharnweber, Vera Hintze, Shahram Ghanaati
Summary: This study aimed to functionalize a collagen-based scaffold with high-sulfated hyaluronan and investigate its properties in vitro and in vivo. The results showed that covalent binding of high-sulfated hyaluronan to collagen released only a marginal amount of the hyaluronan, while electrostatic binding released more. The covalent binding scaffolds showed reduced release of vascular endothelial growth factor and transforming growth factor beta 1, but increased release of interleukin-8 and epithelial growth factor compared to other scaffolds. In vivo, the covalent binding scaffolds reduced the proinflammatory macrophage response and did not induce multinucleated giant cell formation, while maintaining a stable structure and integration into the implantation region. This functionalized collagen scaffold has potential as a stable biomaterial for full-thickness skin regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Linda A. Liang, Thomas Einzmann, Arno Franzen, Katja Schwarzer, Gunther Schauberger, Dirk Schriefer, Kathrin Radde, Sylke R. Zeissig, Hans Ikenberg, Chris J. L. M. Meijer, Charles J. Kirkpatrick, Heinz Koelbl, Maria Blettner, Stefanie J. Klug
Summary: The study compared the strategies of stand-alone HPV testing and cotesting for cervical cancer screening in a population-based study in Germany. The results showed that cotesting had higher sensitivity but also resulted in more false positive results and colposcopy referrals compared to stand-alone HPV testing.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2021)