Journal
THROMBOSIS RESEARCH
Volume 122, Issue 6, Pages 854-860Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.thromres.2008.02.010
Keywords
Endometriosis; Fibrinolysis; Plasminogen activator inhibitor-1; (PAI-1); PAI-1 4G/5G polymorphism; PAI-1mRNA
Categories
Funding
- Plan Nacional de Investigacion Cientifica
- Desarrollo e Innovacion Tecnologica
- Instituto de Salud Carlos III
- Fondo de Investigacion Sanitaria, Madrid [FIS PI050799, PI050844]
- Red RECAVA [RD06/0014/0004]
- Beca Fundacion Espanola de Trombosis y Hemostasia and Beca Fundacion Salud 2000 (Serono), Spain
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Introduction: Endometriosis is a benign gynecologic disease with a high prevalence. It is a multifactorial. and polygenic entity in which the fibrinolytic system may be implicated. The objective of this study was to evaluate the plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism in a group of women with and without endometriosis and to analyze the influence of this polymorphism in PAI-1 expression in endometrial tissue and peritoneal. fluid. Material and methods: In 389 women (170 patients with endometriosis and 219 controls) PAI-1 4G/5G polymorphism was determined by PCR amplification using allele-specific primers. Quantitative real-time RT-PCR assay was used to quantify PAI-1 mRNA and PAI-1 antigen (ag) levels were quantified by ELISA. Results: The genotype and allele frequencies of PAI-1 4G/5G polymorphism did not differ significantly between patients and controls. Control women with the 4G/4G genotype had higher endometrial PAI-1ag (P=0.026) and mRNA (P=0.014) levels than those with the 5G/5G genotype. Control carrying the 4G/4G genotype tended to have higher peritoneal fluid PAI-1ag levels than those carrying the 5G/5G genotype. Moreover, PAI-1ag levels in peritoneal fluid were higher in patients than in controls (P=0.003). Conclusions: The PAI-1 genotype distribution was similar in patients and controls. PAI-1 levels in endometrial tissue and peritonea( fluid seem to be associated With PAI-1 4G/5G polymorphism in controls. The increased PAI-1ag levels observed in peritoneal fluid from patients could contribute to increase the peritoneat adhesions observed in endometriosis. (C) 2008 Elsevier Ltd. All rights reserved.
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